Porphyromonas gingivalis is a major pathogenic oral bacterium that is responsible for periodontal disease. It is linked to chronic periodontitis, gingivitis and aggressive periodontitis. P. gingivalis exerts its pathogenic effects through mechanisms such as immune evasion and tissue destruction, primarily by secreting various factors, including cysteine proteases such as gingipain K (Kgp), gingipain R (RgpA and RgpB) and PrtH (UniProtKB ID P46071). Virulence proteins comprise multiple domains, including the pro-peptide region, catalytic domain, K domain, R domain and DUF2436 domain. While there is a growing database of knowledge on virulence proteins and domains, there was no prior evidence or information regarding the structure and biological function of the well conserved DUF2436 domain. In this study, the DUF2436 domain of PrtH from P. gingivalis (PgDUF2436) was determined at 2.21 Å resolution, revealing a noncanonical β-jelly-roll sandwich topology with two antiparallel β-sheets and one short α-helix. Although the structure of PgDUF2436 was determined by the molecular-replacement method using an AlphaFold model structure as a template, there were significant differences in the positions of β1 between the AlphaFold model and the experimentally determined PgDUF2436 structure. The Basic Local Alignment Search Tool sequence-similarity search program showed no sequentially similar proteins in the Protein Data Bank. However, DaliLite search results using structure-based alignment revealed that the PgDUF2436 structure has structural similarity Z-scores of 5.9–5.4 with the C-terminal domain of AlgF, the D4 domain of cytolysin, IglE and the extracellular domain structure of PepT2. This study has elucidated the structure of the DUF2436 domain for the first time and a comparative analysis with similar structures has been performed.

Structures of tetra­pyridine­silver(I) hexa­fluoro­phosphate and tetra­pyridine silver(I) hexa­fluoro­anti­monate are reported from data collected at 300 K and 100 K.

Light–oxygen–voltage (LOV) domains are small photosensory flavoprotein modules that allow the conversion of external stimuli (sunlight) into intra­cellular signals responsible for various cell behaviors (e.g. phototropism and chloro­plast relocation). This ability relies on the light-induced formation of a covalent thio­ether adduct between a flavin chromophore and a reactive cysteine from the protein environment, which triggers a cascade of structural changes that result in the activation of a serine/threonine (Ser/Thr) kinase. Recent developments in time-resolved crystallography may allow the activation cascade of the LOV domain to be observed in real time, which has been elusive. In this study, we report a robust protocol for the production and stable delivery of microcrystals of the LOV domain of phototropin Phot-1 from Chlamydomonas reinhardtii (CrPhotLOV1) with a high-viscosity injector for time-resolved serial synchrotron crystallography (TR-SSX). The detailed process covers all aspects, from sample optimization to data collection, which may serve as a guide for soluble protein preparation for TR-SSX. In addition, we show that the crystals obtained preserve the photoreactivity using infrared spectroscopy. Furthermore, the results of the TR-SSX experiment provide high-resolution insights into structural alterations of CrPhotLOV1 from Δt = 2.5 ms up to Δt = 95 ms post-photoactivation, including resolving the geometry of the thio­ether adduct and the C-terminal region implicated in the signal transduction process.

The reaction of Zn(ClO4)2·6H2O with Na3SbS4·9H2O in a water/aceto­nitrile mixture leads to the formation of the title compound, (μ-tetra­thio­anti­monato-κ2S:S')bis­[(1,4,8,11-tetra­aza­cyclo­tetra­decane-κ4N)zinc(II)] perchlorate 0.8-hydrate, [Zn2(SbS4)(C10H24N4)2]ClO4·0.8H2O or [(Zn-cyclam)2(SbS4)]+[ClO4]−·0.8H2O. The asymmetric unit consists of two crystallographically independent [SbS4]3– anions, two independent perchlorate anions and two independent water mol­ecules as well as four crystallographically independent Zn(cyclam)2+ cations that are located in general positions. Both perchlorate anions and one cyclam ligand are disordered and were refined with a split mode using restraints. The water mol­ecules are partially occupied. Two Zn(cyclam)2+ cations are linked via the [SbS4]3– anions into [Zn2(cyclam)2SbS4]+ cations that are charged-balanced by the [ClO4]− anions. The water mol­ecules of crystallization are hydrogen bonded to the [SbS4]3– anions. The cations, anions and water mol­ecules are linked by N—H⋯O, N—H⋯S and O—H⋯S hydrogen bonds into a three-dimensional network. Powder X-ray diffraction proves that a pure sample had been obtained that was additionally investigated for its spectroscopic properties.

Although there is evidence of harmful health and ecological effects associated with exposure to high doses of chemicals known as hormonally active agents – or endocrine disrupters – little is understood about the harm posed by exposure to the substances at low concentrations, such as those that typically exist in the environment, says a new report from a National Research Council committee.

Chicago-based Mike’s Hard Lemonade Co. is partnering with actor Lamorne Morris to host an interactive pop-up event: Mike’s Jobstacle Course. This immersive experience will prove that every job has its hard days. And a hard day deserves a refreshing-tasting Mike’s Hard Lemonade, the company says.

The company notes that these functional beverages provide both energy and the “added benefit of mental focus.” The 12-ounce servings contain 100 mg of natural caffeine, as well as 100 mg of natural L-theanine.

Guayakí Yerba Mate introduced its latest flavor innovation: Berry Lemonade, joining the brand’s High Energy Cans on shelves nationwide.

Celsius Holdings Inc. announced two new refreshing flavors inspired by summertime: CELSIUS Sparkling Watermelon Lemonade and CELSIUS Sparkling Kiwi Strawberry.

ROAR Organic unveiled its newest offerings: Cherry Limeade Plus Powder and Blackberry Lemonade, ROAR’s latest flavor addition to its ready-to-drink beverage line. 

The Community Organization Based out of Nebraska Grants ALSF nearly $69,000.

Thousands of decision makers from across the private capital universe will come together in Monaco, November 5-8, for a SuperInvestor like never before. Nico Rosberg shifts into high gear for the event.

The ebook version of Little Bit Of Faith will be available at no charge August 20 through August 21, 2024 at Amazon.

Dr. Mona Mofid has been recognized as a 2023 Castle Connolly Top Doctor — the ninth year receiving this prestigious Top Doctor honor.

San Diego Magazine and San Diego Medical Society Top Doctor 2023 Award

Crow writes meticulously researched, entertaining novels of romance, history, adventure and mystery in an engaging you-are-there style that allows readers to live the history. Reviewers have compared her work with PD James, Dan Brown and Barbara Pym.

Saverio Monachino amalgamates an odd collection of authors like John Irving, Tom Robbins, and Louise Penny into one, and the servings he presents—psychological fiction thrillers—come complete with a side order of comedy. Saverio believes adding

Saverio Monachino amalgamates an odd collection of authors like John Irving, Tom Robbins, and Louise Penny into one, and the servings he presents—psychological fiction thrillers—come complete with a side order of comedy.

Dr. Romona Jackson Jones, EdD, is a groundbreaking public servant in Georgia's Douglas County

Saverio Monachino amalgamates an odd collection of authors like John Irving, Tom Robbins, and Louise Penny into one, and the servings he presents come complete with a side order of comedy.

Nov 13, 2024, 3pm EST

Join a conversation with physician, scientist, and the activist who exposed the Flint water crisis, Dr. Mona Hanna-Attisha. Dr. Mona is the associate dean for public health and C.S. Mott Endowed Professor of Public Health at Michigan State University College of Human Medicine, and the founding director of the Pediatric Public Health Initiative, a public health program of MSU and Hurley Children’s Hospital in Flint, Michigan.

Named one of Time Magazine’s 100 Most Influential People in the World and recognized as one of USA Today’s Women of the Century for her role in uncovering the Flint water crisis and leading recovery efforts, Dr. Mona has testified before the U.S. Congress and advocates for children in Flint and beyond.

Online Location Details: tufts.zoom.us…
Campus: Boston Health Sciences campus
Location Details: DuBlois Auditorium
Open to Public: Yes
Event Type: Lecture/Presentation/Seminar/Talk
Event Sponsor Details: Tufts University School of Medicine
Event Contact Name: Jessica Byrnes, Senior Communications Manager
Event Contact Email: jessica.byrnes@tufts.edu
More info: tischcollege.tufts.edu…

The Australian artist performs songs from her latest album.

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The TOPPAN booth will focus on cosmetics, featuring a lineup of digital solutions addressing the challenges faced by the luxury cosmetics industry, such as achieving both brand differentiation and labor-saving as well as enhancing customer service.

La marcha por los derechos de gays y lesbianas, toda una fiesta en Estambul, pone bajo los focos a las transexuales que, pese a la avanzada legislación de Turquía, siguen sufriendo agresiones y discriminación incluso por parte de las autoridades.

La Guardia Civil detuvo al presunto agresor durante la madrugada. Uno de los religiosos se encuentra en esta crítico y otro grave Leer

El fraile había sido dado por muerto por el Arzobispado el sábado debido a las heridas, pero la Guardia Civil confirmó que su estado era crítico. El agresor fue detenido ese mismo día. En total agredió a cinco religiosos Leer

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884: The Attack on the Monastery of Dakil Ishu

1072: The Massacre of the Monks of Kamul Monastery

1072 - The sack of the Monastery of Kamul

Mor Augin Monastery’s Land in Tur-Abdin seized by Kurds

Cuidados de la fibrosis pulmonar idiopática.

Let the queen conquer.

The post Wives of Soviet Cosmonauts appeared first on English Russia.

The beloved butterflies had fallen to critical levels in recent years. Experts weigh in on what might be causing their remarkable return.

Frank C. Nichols
Dec 1, 2020; 61:1645-1657
Research Articles

Porphyromonas gingivalis is a Gram-negative anaerobic periodontal microorganism strongly associated with tissue-destructive processes in human periodontitis. Following oral infection with P. gingivalis, the periodontal bone loss in mice is reported to require the engagement of Toll-like receptor 2 (TLR2). Serine-glycine lipodipeptide or glycine aminolipid classes of P. gingivalis engage human and mouse TLR2, but a novel lipid class reported here is considerably more potent in engaging TLR2 and the heterodimer receptor TLR2/TLR6. The novel lipid class, termed Lipid 1256, consists of a diacylated phosphoglycerol moiety linked to a serine-glycine lipodipeptide previously termed Lipid 654. Lipid 1256 is approximately 50-fold more potent in engaging TLR2 than the previously reported serine-glycine lipid classes. Lipid 1256 also stimulates cytokine secretory responses from peripheral blood monocytes and is recovered in selected oral and intestinal Bacteroidetes organisms. Therefore, these findings suggest that Lipid 1256 may be a microbial TLR2 ligand relevant to chronic periodontitis in humans.

When monarch butterflies migrate, they produce one of the most iconic wildlife spectacles in the world — and provide us with an important indicator of ecological health, says photographer Jaime Rojo. Telling a story about our relationship to the natural world, he shares his experience photographing these mesmerizing insects deep in their remote mountain habitats in Mexico, diving into the latest research into the mysteries of their multi-thousand-mile journey and sharing how each of us can join the growing movement to protect them.

A local Chinese mother teaches Cantonese to three Pakistani mothers before they all watch the film 'Magdalena: Released from Shame' together.

Pulmonary hypertension (PH) is highly prevalent in patients with interstitial lung disease (ILD) and is associated with increased morbidity and mortality. Widely available noninvasive screening tools are warranted to identify patients at risk for PH, especially severe PH, that could be managed at expert centres. This review summarises current evidence on noninvasive diagnostic modalities and prediction models for the timely detection of PH in patients with ILD. It critically evaluates these approaches and discusses future perspectives in the field. A comprehensive literature search was carried out in PubMed and Scopus, identifying 39 articles that fulfilled inclusion criteria. There is currently no single noninvasive test capable of accurately detecting and diagnosing PH in ILD patients. Estimated right ventricular pressure (RVSP) on Doppler echocardiography remains the single most predictive factor of PH, with other indirect echocardiographic markers increasing its diagnostic accuracy. However, RVSP can be difficult to estimate in patients due to suboptimal views from extensive lung disease. The majority of existing composite scores, including variables obtained from chest computed tomography, pulmonary function tests and cardiopulmonary exercise tests, were derived from retrospective studies, whilst lacking validation in external cohorts. Only two available scores, one based on a stepwise echocardiographic approach and the other on functional parameters, predicted the presence of PH with sufficient accuracy and used a validation cohort. Although several methodological limitations prohibit their generalisability, their use may help physicians to detect PH earlier. Further research on the potential of artificial intelligence may guide a more tailored approach, for timely PH diagnosis.

BACKGROUND:Pulmonary function tests (PFTs) have historically used race-specific prediction equations. The recent American Thoracic Society guidelines recommend the use of a race-neutral approach in prediction equations. There are limited studies centering the opinions of practicing pulmonologists on the use of race in spirometry. Provider opinion will impact adoption of the new guideline. The aim of this study was to ascertain the beliefs of academic pulmonary and critical care providers regarding the use of race as a variable in spirometry prediction equations.METHODS:We report data from 151 open-ended responses from a voluntary, nationwide survey (distributed by the Association of Pulmonary Critical Care Medicine Program Directors) of academic pulmonary and critical care providers regarding the use of race in PFT prediction equations. Responses were coded using inductive and deductive methods, and a thematic content analysis was conducted.RESULTS:There was a balanced distribution of opinions among respondents supporting, opposing, or being unsure about the incorporation of race in spirometry prediction equations. Responses demonstrated a wide array of understanding related to the concept and definition of race and its relationship to physiology.CONCLUSIONS:There was no consensus among providers regarding the use of race in spirometry prediction equations. Concepts of race having biologic implications persist among pulmonary providers and will likely affect the uptake of the Global Lung Function Initiative per the American Thoracic Society guidelines.

Extract

In cystic fibrosis (CF), Pseudomonas aeruginosa acquisition represents a turning point in disease progression. The presence of chronic P. aeruginosa infection is associated with worsening lung function and increased risk of earlier death, whereas treatment substantially improves lung function and survival [1, 2]. Efforts to diagnose and eradicate early P. aeruginosa provide lasting benefits for children with CF [3, 4]. However, the timing of infection varies considerably between individuals with CF, treatment centres [5, 6], and different birth cohorts of people with the disease [7, 8].

Background

Pseudomonas aeruginosa is a common pathogen that contributes to progressive lung disease in cystic fibrosis (CF). Genetic factors other than CF-causing CFTR (CF transmembrane conductance regulator) variations contribute ~85% of the variation in chronic P. aeruginosa infection age in CF according to twin studies, but the susceptibility loci remain unknown. Our objective is to advance understanding of the genetic basis of host susceptibility to P. aeruginosa infection.

ABSTRACTWe document the effort over the last 30 years to respond to the call by women advocates at the International Conference on Population and Development for more woman-initiated single or dual-purpose contraceptive methods by developing the Caya contoured diaphragm, an innovative diaphragm designed to meet the needs of women and their partners and expand options for nonhormonal barrier contraception. We describe the complex and interrelated set of activities undertaken to develop the product using a human-centered design process and how we are working to create a corollary sustainable market. This review includes the evidence generated around improved acceptability among couples in low- and middle-income countries and depicts challenges and practical actions on how to dispel misconceptions about diaphragm use. Importantly, we share programmatic lessons learned on increasing universal access to this new sexual and reproductive health technology. Following our new model for increasing access to new and underutilized methods, Caya is now registered and being marketed in nearly 40 countries worldwide.