PIC16(L)F18854 Family Silicon Errata and Data Sheet Clarification

PIC16(L)F18857/18877 Family Silicon Errata and Data Sheet Clarification

PIC12LF1840T48A

PIC12LF1840T39A

PIC16LF1824T39A

PIC18F14Q41

PIC18F16Q41

PIC18F15Q41

PIC18F16Q40

PIC18F14Q40

PIC18F15Q40

Replication initiation in eukaryotic cells occurs asynchronously throughout S phase, yielding early- and late-replicating regions of the genome, a process known as replication timing (RT). RT changes during development to ensure accurate genome duplication and maintain genome stability. To understand the relative contributions that cell lineage, cell cycle, and replication initiation regulators have on RT, we utilized the powerful developmental systems available in Drosophila melanogaster. We generated and compared RT profiles from mitotic cells of different tissues and from mitotic and endocycling cells of the same tissue. Our results demonstrate that cell lineage has the largest effect on RT, whereas switching from a mitotic to an endoreplicative cell cycle has little to no effect on RT. Additionally, we demonstrate that the RT differences we observed in all cases are largely independent of transcriptional differences. We also employed a genetic approach in these same cell types to understand the relative contribution the eukaryotic RT control factor, Rif1, has on RT control. Our results demonstrate that Rif1 can function in a tissue-specific manner to control RT. Importantly, the Protein Phosphatase 1 (PP1) binding motif of Rif1 is essential for Rif1 to regulate RT. Together, our data support a model in which the RT program is primarily driven by cell lineage and is further refined by Rif1/PP1 to ultimately generate tissue-specific RT programs.

Peripheral somatosensory input is modulated in the dorsal spinal cord by a network of excitatory and inhibitory interneurons. PTF1A is a transcription factor essential in dorsal neural tube progenitors for specification of these inhibitory neurons. Thus, mechanisms regulating Ptf1a expression are key for generating neuronal circuits underlying somatosensory behaviors. Mutations targeted to distinct cis-regulatory elements for Ptf1a in mice, tested the in vivo contribution of each element individually and in combination. Mutations in an autoregulatory enhancer resulted in reduced levels of PTF1A, and reduced numbers of specific dorsal spinal cord inhibitory neurons, particularly those expressing Pdyn and Gal. Although these mutants survive postnatally, at ~3–5 wk they elicit a severe scratching phenotype. Behaviorally, the mutants have increased sensitivity to itch, but acute sensitivity to other sensory stimuli such as mechanical or thermal pain is unaffected. We demonstrate a requirement for positive transcriptional autoregulatory feedback to attain the level of the neuronal specification factor PTF1A necessary for generating correctly balanced neuronal circuits.

We characterized 29 bla_CTX-M-27-harboring plasmids of Escherichia coli sequence type 131 (ST131) sublineage C1/H30R isolates from healthy individuals and long-term-care facility (LTCF) residents. Most (27/29) plasmids were of the FIA, FIB, and FII multireplicon type with the same plasmid multilocus sequence typing (pMLST). Several plasmids (7/23) from LTCF residents harbored only bla_CTX-M-27 as the resistance gene; however, their fundamental structures were very similar to those of previously isolated bla_CTX-M-27/F1:A2:B20 plasmids, suggesting their prevalence as a newly arising public health concern.

The last Formula One season was a disappointing one for Daniel Ricciardo, who retains a burning desire to win the world title.

F1 remains hopeful that the season can start in early July with a double header at the Austrian Grand Prix, and that 15-18 of the 22 scheduled races can yet be completed. “It’s going to be very empty," Hamilton said Saturday, evoking the subdued atmosphere of pre-season testing in Spain. “For us it’s going to be like a test day, probably even worse than a test day in a sense," the Mercedes driver said.

• Alex Wurz calls measures to deal with coronavirus ‘immaculate’
  
• ‘F1 can be pioneer’ for other sports to follow, says GPDA chief

The Grand Prix Drivers’ Association is confident the precautions being taken by Formula One mean the sport stands every chance of resuming as planned in Austria on 5 July.

Alex Wurz, chairman of the GPDA, will reassure his members that Formula One has put the safety of everyone involved at the forefront of its plans to return to racing after attending a meeting with F1 and the FIA on Friday.

A leading voice in Formula One warns there is a serious threat of losing teams if big changes are not made to the competition.

Bernie Ecclestone has reportedly demanded a doubling of the annual charge to US$26 million if the Turkish Grand Prix is to remain

The beleaguered US F1 outfit has taken another hit after one of its sponsors, Locstein, confirmed it had withdrawn its support

The FIA has sent technical delegate Charlie Whiting to inspect US F1's factory, after reports suggested the Charlotte-based squad is way behind on its chassis build and in financial trouble

US F1 and Stefan GP are rumoured to be in merger talks to ensure a thirteenth team is present at the first race of the season in Bahrain

Stefan GP will reveal its 2010 car next week in the hope that the FIA will allow it to take the place of any no-shows in this year's championship

Stefan GP has made one final plea to be accepted into the world championship, after it emerged that merger talks with US F1 had fallen through

The manager of the only driver currently under contract with US F1, Jose Maria Lopez, is working to extricate his client from the team

Jose Maria 'Pechito' Lopez will not take part in Formula One this year after his manager revealed that he had not been able to land a reserve driver role at HRT

A company that hired two engineers from the failed 2010 entrant US F1 says the team still wants to race in Formula One

A disappointed Jose Maria Lopez has not ruled out mounting a new Formula One foray in the future

Serbian businessman Zoran Stefanovic has not abandoned his ambition for his Stefan GP team to race this season, according to a report on Autosport

US F1 has ceased operation, laying off its entire staff indefinitely and closing its factory

US F1 will not launch a renewed bid to debut in Formula One in 2011

The auction of the US F1's assets has raised $1.4 million for its creditors

As the European air chaos continues due to the Icelandic volcano cloud, Lotus has booked its entire race team on flights to Malaysia

Bernie Ecclestone believes Formula One would be better off with 10 teams as the grid looks set to be reduced to 11 in 2012

Michael Schumacher's 15-year-old son Mick wants to follow in his father's famous footsteps and be a Formula One world champion after being crowned World Karting Vice-Champion

• World champion not relishing racing without fans
• Hamilton appreciates sport’s importance to many

Lewis Hamilton believes returning to grand prix racing without fans will be an “empty” experience as Formula One prepares to launch the new season behind closed doors.

F1 expects to hold its first race on 5 July in Austria as a double header followed by two meetings at Silverstone, all without spectators. However, there remains the possibility that government quarantine restrictions may make travel for F1 teams unfeasible.

Mercedes driver Lewis Hamilton says he feels certain benefits from his enforced time away from Formula 1.

A decision on whether to go ahead with June's French Formula One Grand Prix behind closed doors or to postpone or scrap the event will be made in the coming days, organisers said Tuesday. The grand prix scheduled for June 28 is the first race on the revised Formula One world championship calendar with nine races already scrapped or suspended as a result of the coronavirus pandemic.

Following French President Emmanuel Macron's announcement Monday that the lockdown in France will be extended until May 11 and public gatherings banned until mid-July, a spokesman for the race organisers told AFP that they "have been studying all scenarios" including postponement or staging the event behind closed doors.

F1 sports director Ross Brawn said last week he could envisage the start of the F1 season in Europe in July with a race excluding the public.

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Four-time world champion Sebastian Vettel on Friday cautioned against the temptation to shoehorn too many Formula One races into a revised calendar, describing the move as "not realistic". The 22-event F1 season has been decimated by the Coronavirus with nine races either cancelled or postponed. The French Grand Prix scheduled for June 28 is also in doubt as is the Belgian Grand Prix, set for August 30.

F1 chiefs have floated the idea of making up for lost time by staging races on successive weekends or even having two races on the same weekend.

Staff burnout issue

However, Ferrari star Vettel fears such a move could lead to burnout for team staff. "We drivers are a little privileged," Vettel told reporters by teleconference from his home in Switzerland. "Of course, the races are tiring but there have to be limits for the staff. They must rest. "We must also see if it is easy to reschedule races, if the circuits are not already taken. Many questions remain. I think the schedule will be busier, but 10 consecutive weekends is not realistic."

Vettel suggested that he would favour staging races without fans if it allowed a quick resumption as long as it did not become a common feature. Other sports have already toyed with the idea of staging events behind closed doors. For example, the US PGA Tour on Thursday announced plans to resume in June, with the first four tournaments being closed to spectators.

"It's complicated," admitted Vettel. "On the one hand, there is the health of the sport, on the other, that of the people who work in the paddock and especially the fans. "There are several options. No one likes to run in front of empty stands, but we will have to see if it will not allow us to resume much sooner. The first races will probably be a little different, but not too much, I hope, because we want to run in front of the fans."

Vettel insists that for him even a 10-race season is just as valuable as a 22-race campaign. However, he admits that the damage to the sport caused by the pandemic could be fatal for the smaller teams on the grid.

'Small teams in danger'

Without racing, the massive TV and sponsorship revenues have dried up. Half of the teams have already started furloughing staff. Teams have agreed to lower the spending cap from $175 million to $150m. "Some small teams are in danger and, as a family, F1 has to take care of its own."

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Ferrari's Charles Leclerc claimed a second victory in the Formula One Esports Virtual Grand Prix championship on Sunday, marking his Shanghai win by cooking pasta. Leclerc started on pole and finished ahead of Red Bull's Alex Albon and Guanyu Zhou, who races in Formula 2.

"I'm actually enjoying very much playing, and streaming. And I enjoy it even more when I win," tweeted 22-year-old Leclerc who also has two wins in the real F1 world championship. "But the post-race celebrations are somehow feeling a bit different. "Switching off the computer and go cook white pasta is a bit less glamour than spraying champagne on the podium."

The Formula One world championship has yet to get underway this season due to the spread of the coronavirus. Nine of the scheduled 22 races have either been cancelled or postponed.

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Formula One organizers are open to renegotiating hosting fees for races that may take place without fans this season because of the coronavirus pandemic, the general manager of the Circuit de Barcelona-Catalunya said. Joan Fontseré told The Associated Press that Liberty Media is ¿conscious that if races need to go on without fans the contracts with event promoters will have to be somehow renegotiated.

"They are conscious that this is an exceptional situation," Fontseré said Tuesday. "We are obviously on the same page. If they want to keep some races on because of the TV rights, because of the teams ... they know that our income (will be reduced), they realize that this year it will be like that, so for sure we are on the same page" said Spanish Grand Prix organizers at this moment are not even considering a race with fans in Barcelona.

He said it's not only ticket sales that would be affected if the event goes on with empty stands and no hospitality suites. "When the Catalan government invests in F1, it's not only for the tickets that we sell, it's also for the financial impact that the event has in the country, in Catalonia," Fontseré said.

The economic impact for the country will be very reduced. It means no income for taxis, for hotels ... so that changes completely the agreement between the two parties." Liberty Media did not immediately respond to a request for comment. The Spanish GP brings in more than 160,000 million euros ($173 million) to the region, with the total of its financial impact during the year nearing 300 million euros ($325 million), according to data from the track. The attendance for last year's race-weekend surpassed 160,000 people.

Fontseré said he received a couple of phone calls from F1 CEO Chase Carey to discuss possible solutions for the Spanish GP. He said Carey said the series is trying to run as many races as possible, but it was still too early to know when the season would actually resume, whether it would be in the summer or only in the fall. Nine of the 22 races have already been postponed or canceled, and F1 recently put half of its staff on furlough until the end of May. Some teams also took similar actions to reduce costs.

F1 organizers have said they hope to hold between 15 and 18 races this year. The Australian GP and the Monaco GP have already been canceled. Fontseré said the Spanish GP is "completely at the disposal" or organizers and is open to all proposals, be it doubleheaders, shortened weekends or almost anything else other than running on a reverse layout, as that would require too many complex changes to the track and could pose safety concerns.

Among the ideas reportedly being discussed in F1 is to have two or three consecutive races at the same circuit and to use fewer days of on-track activities. There were also talks about changing the format of qualifying and even races. "We need to reduce two things: costs and risks," Fontseré said. "So the fewer people we move, the smaller the risk, and the fewer days we use and the fewer activities we do, the lower the costs. It's an exceptional season and exceptional decisions need to be taken."

He said it is key to have as many races as possible this year in order to have a strong 2021 season, but he would understand if the Spanish GP was eventually left off the calendar. He was optimistic with its chances, though, considering its tradition, infrastructure and location. Fontseré said he can get the Barcelona-Catalunya track ready for a race in "two to three weeks" and expects the Spanish GP to be among the first to resume.

"As soon as we can restart the season, I'm sure that it will be with European races," he said, "and we will be around there."

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