CRUISE holidays have been hugely impacted by the coronavirus crisis with cruises cancelled left, right and centre as the pandemic batters the travel industry. Now a fresh blow comes as two major cruise lines announce cruises won't be returning until autumn.

Federal officials will hold virtual meetings on a contested plan to guide oil and gas development near Chaco Culture National Historical Park.

Utah's pace of applications for unemployment benefits is slowing, but still at historic highs, according to figures released Thursday.

The world is nearing 4 million cases of the coronavirus. More COVID-19 news Saturday.

Hamilton County Sheriff's Office is investigating the death of a man who was found in a ditch Friday afternoon just northeast of Noblesville.

A male suspect was shot during an exchange of gunfire with police at North Michigan Road and West 62nd Street.

How the beloved bulldog would've been at the center of a national championship celebration; well, he got a pet from Peyton Manning in the deal

Richard Wood led Eli Lilly and Co. through prosperous times, thanks to products like Prozac. Colleagues say his foresight paid off.

Several Indianapolis-area restaurants are offering brunch deals and takeout specials to help mom relax and stay out of the kitchen on Mother's Day.

These Indianapolis-area spots have some delicious sandwiches.

How the beloved bulldog would've been at the center of a national championship celebration; well, he got a pet from Peyton Manning in the deal

Officers were called to East 38th Street and N. Mitthoeffer Road shortly before 10 p.m. where they found a body lying in the roadway.

A trough off the West Coast is expected to bring a robust cooling trend to California next week.

A chronology of key events

Key facts, figures and dates

Scientists map ancient underwater landslides in the region chosen for Jakarta's replacement.

An unseen object is found to be lurking in a double-star system a mere 1,000 light-years from Earth.

A chronology of key events

A chronology of key events

A chronology of key events

Key facts, figures and dates

Key facts, figures and dates

Key facts, figures and dates

For the first time in six weeks, Gatineau Park is open to the public during the COVID-19 pandemic.

A Chicago cyclist was injured by an electric scooter rider who fled the scene. Now he's gone to court to get the city's scooter companies to turn over personal information about their riders and their history.

The 30-year fixed-rate average moved slightly higher this week, increasing to 3.26 percent.

I am not suggesting, or recommending, you should sneak booze on your next next cruising vacation as you could, in worst case, risk being refused boarding if caught. However despite this, there are cruisers who use surprising and unusual techniques to smuggle alcohol on to a cruise ship to avoid having to buy drinks on board. In this video I explore the different ways that people are using to sneak their alcohol onto their cruise ship. The cost of drinking on a cruise is increasing, and with some of the drinks packages you could be spending almost as much as the cruise itself costs and some travellers are resorting to innovative methods to bring their own alcohol onto cruise ships. There are even companies making products, devices and accessories specifically designed to assist.

BitTorrent founder Bram Cohen and his company are moving forward with its P2P live streaming project, expanding field trials and courting indie bands to stress test Cohen’s algorithms. However, it could still take months before BitTorrent Live is ready for prime time. Continue reading on NewTeeVee.

An arson case is being investigated after a tavern was torched in Hambanathi, Tongaat, outside Durban, following the discovery of human remains in the nearby Wewe River, KwaZulu-Natal police say.

Borrowing costs have stayed at or near record lows for five consecutive weeks.

The Amateur Traveler talks to Sarah from Whitehorse in the Yukon about that corner of Canada with long winters but dramatic scenery. She tells of of Winter festivals like the Yukon Sourdough Rendezvous as well as Summer festivals like Dawson City Music fest, Atlin Arts and Music Fest and the Kluane Mountain Bluegrass Festival. We talk about sporting events like the 24 Hours of Light and Kluane to Chilkat (Haines to Haines) bike races because Sarah says you have to like the outdoors to come to the Yukon. Sarah also gives us some itineraries for road warriors like the Top of the World Highway to Dawson (and the Dempster Highway for the bold among you) and the Golden Circle route into Alaska. We walk the Chilkoot Trail but without the requirement to bring 1,000 pounds of supplies like in the Yukon gold rush. It is the beauty of Kluane National Park that you will remember but strangely enough it was something else that Sarah was looking at when she decided to move to the Yukon.

The Amateur Traveler talks to Beth Whitman of Waderlust and Lipstick about her recent Papua New Guinea. Beth was attracted to this remote country that produced more languages than any other country because of people separated by its rugged terrain. She tells us about the wonder, beauty and yes danger of Papua New Guinea. Beth was preparing to take a tour to the island nation and to the Mount Hagen Show which is a sing sing which allows the different tribes to compete with songs and costumes.

The Amateur Traveler talks to Kirk Horsted in a two part episode on the Twin Cities of Minneapolis and Saint Paul, Minnesota. This first episode focuses on Minneapolis with day trips west of the Mississippi river.

Hear about a western Mediterranean cruise to Spain, Gibraltar, France, Monaco and Italy on Holland America's Westerdam where I just attended a press trip.

Hear about National Parks in Washington D.C. and the vicinity as the Amateur Traveler talks to Erik Smith about monuments, memorials, mansions, battlefields, gardens and more.

Eleven members of the Secret Service have tested positive for COVID-19, according a new report. Yahoo News reported Friday it has seen Department of Homeland Security documents which show that 11 individuals currently have the virus and that 23 members of the Secret Service have recovered from the disease. Another 60 employees of the agency…

The post Report: Secret Service Discovers Nearly a Dozen Coronavirus Cases in Its Own Ranks appeared first on The Western Journal.

RCMP say a 50-year-old man died and a 60-year-old man is in hospital following a crash involving a CP Rail train Friday morning.

Joshua Summers, CEO of linear television supply-side platform clypd, discusses the major advanced targeting trends expected to infiltrate traditional TV ad buying within the next year.

Despite strong growth, D2C companies are facing more headwinds   April 2, 2020 (New York, NY) — For the first time, we are breaking out direct-to-consumer (D2C) ecommerce sales. We define […]

Manitoba’s unemployment rate nearly doubled between March and April, according to the monthly report from Statistics Canada released Friday morning.

12 December 2016

Pierre Lairez
J. Amer. Math. Soc. 33 (2019), 487-526.
Abstract, references and article information

Members Event

Event participants

HE George Vella, President, Republic of Malta

Chair: Dr Alex Vines OBE, Managing Director, Ethics, Risk & Resilience; Director, Africa Programme, Chatham House

T-type (Cav3) Ca2+ channels are important regulators of excitability and rhythmic activity of excitable cells. Among other voltage-gated Ca2+ channels, Cav3 channels are uniquely sensitive to oxidation and zinc. Using recombinant protein expression in HEK293 cells, patch clamp electrophysiology, site-directed mutagenesis, and homology modeling, we report here that modulation of Cav3.2 by redox agents and zinc is mediated by a unique extracellular module containing a high-affinity metal-binding site formed by the extracellular IS1–IS2 and IS3–IS4 loops of domain I and a cluster of extracellular cysteines in the IS1–IS2 loop. Patch clamp recording of recombinant Cav3.2 currents revealed that two cysteine-modifying agents, sodium (2-sulfonatoethyl) methanethiosulfonate (MTSES) and N-ethylmaleimide, as well as a reactive oxygen species–producing neuropeptide, substance P (SP), inhibit Cav3.2 current to similar degrees and that this inhibition is reversed by a reducing agent and a zinc chelator. Pre-application of MTSES prevented further SP-mediated current inhibition. Substitution of the zinc-binding residue His191 in Cav3.2 reduced the channel's sensitivity to MTSES, and introduction of the corresponding histidine into Cav3.1 sensitized it to MTSES. Removal of extracellular cysteines from the IS1–IS2 loop of Cav3.2 reduced its sensitivity to MTSES and SP. We hypothesize that oxidative modification of IS1–IS2 loop cysteines induces allosteric changes in the zinc-binding site of Cav3.2 so that it becomes sensitive to ambient zinc.