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In situ counter-diffusion crystallization and long-term crystal preservation in microfluidic fixed targets for serial crystallography

Compared with batch and vapor diffusion methods, counter diffusion can generate larger and higher-quality protein crystals yielding improved diffraction data and higher-resolution structures. Typically, counter-diffusion experiments are conducted in elongated chambers, such as glass capillaries, and the crystals are either directly measured in the capillary or extracted and mounted at the X-ray beamline. Despite the advantages of counter-diffusion protein crystallization, there are few fixed-target devices that utilize counter diffusion for crystallization. In this article, different designs of user-friendly counter-diffusion chambers are presented which can be used to grow large protein crystals in a 2D polymer microfluidic fixed-target chip. Methods for rapid chip fabrication using commercially available thin-film materials such as Mylar, propyl­ene and Kapton are also detailed. Rules of thumb are provided to tune the nucleation and crystal growth to meet users' needs while minimizing sample consumption. These designs provide a reliable approach to forming large crystals and maintaining their hydration for weeks and even months. This allows ample time to grow, select and preserve the best crystal batches before X-ray beam time. Importantly, the fixed-target microfluidic chip has a low background scatter and can be directly used at beamlines without any crystal handling, enabling crystal quality to be preserved. The approach is demonstrated with serial diffraction of photoactive yellow protein, yielding 1.32 Å resolution at room temperature. Fabrication of this standard microfluidic chip with commercially available thin films greatly simplifies fabrication and provides enhanced stability under vacuum. These advances will further broaden microfluidic fixed-target utilization by crystallographers.




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Free tools for crystallographic symmetry handling and visualization

Online courses and innovative teaching methods have triggered a trend in education, where the integration of multimedia, online resources and interactive tools is reshaping the view of both virtual and traditional classrooms. The use of interactive tools extends beyond the boundaries of the physical classroom, offering students the flexibility to access materials at their own speed and convenience and enhancing their learning experience. In the field of crystallography, there are a wide variety of free online resources such as web pages, interactive applets, databases and programs that can be implemented in fundamental crystallography courses for different academic levels and curricula. This paper discusses a variety of resources that can be helpful for crystallographic symmetry handling and visualization, discussing four specific resources in detail: the Bilbao Crystallographic Server, the Cambridge Structural Database, VESTA and Jmol. The utility of these resources is explained and shown by several illustrative examples.




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Towards dynamically configured databases for CIFs: the new modulated structures open database at the Bilbao Crystallographic Server

This article presents a web-based framework to build a database without in-depth programming knowledge given a set of CIF dictionaries and a collection of CIFs. The framework consists of two main elements: the public site that displays the information contained in the CIFs in an ordered manner, and the restricted administrative site which defines how that information is stored, processed and, eventually, displayed. Thus, the web application allows users to easily explore, filter and access the data, download the original CIFs, and visualize the structures via JSmol. The modulated structures open database B-IncStrDB, the official International Union of Crystallography repository for this type of material and available through the Bilbao Crystallographic Server, has been re-implemented following the proposed framework.




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X-ray-based technologies in emerging fuel cell research

The workshop titled `X-ray-based technologies in emerging fuel cell research', organized by Vivian Stojanoff from Brookhaven National Laboratory (BNL) and Narayanasami Sukumar from Cornell University/Advanced Photon Source-Northeastern Collaborative Access Team, was a notable segment of the National Synchrotron Light Source II and Center for Functional Nanomaterials Users' Meeting held 13–17 May 2024. This one-day event, on 13 May 2024, at BNL in New York, aimed to bring together researchers, beamline scientists, management and developers to propel fuel cell technology forward using model systems inspired by natural photosynthesis and redox enzymes. This summary encapsulates the key discussions, advancements and future implications of the workshop.




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SUBGROUPS: a computer tool at the Bilbao Crystallographic Server for the study of pseudo-symmetric or distorted structures

SUBGROUPS is a free online program at the Bilbao Crystallographic Server (https://www.cryst.ehu.es/). It permits the exploration of all possible symmetries resulting from the distortion of a higher-symmetry parent structure, provided that the relation between the lattices of the distorted and parent structures is known. The program calculates all the subgroups of the parent space group which comply with this relation. The required minimal input is the space-group information of the parent structure and the relation of the unit cell of the distorted or pseudo-symmetric structure with that of the parent structure. Alternatively, the wavevector(s) observed in the diffraction data characterizing the distortion can be introduced. Additional conditions can be added, including filters related to space-group representations. The program provides very detailed information on all the subgroups, including group–subgroup hierarchy graphs. If a Crystallographic Information Framework (CIF) file of the parent high-symmetry structure is uploaded, the program generates CIF files of the parent structure described under each of the chosen lower symmetries. These CIF files may then be used as starting points for the refinement of the distorted structure under these possible symmetries. They can also be used for density functional theory calculations or for any other type of analysis. The power and efficiency of the program are illustrated with a few examples.




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Twinning and homo-epitaxy cooperation in the already rich growth morphology of CaCO3 polymorphs. II. Calcite

The two most abundant CaCO3 polymorphs, calcite and aragonite, are universally recognized for the richness of their morphology to which different twins make relevant contributions. The epitaxial transformation calcite ↔ aragonite has long been debated. While the twinning has been thoroughly treated, the homo-epitaxy occurring within each of these minerals has, inexplicably, been overlooked to date, both experimentally and theoretically. Twinning can be deceptive to the point where it can be mistaken for homo-epitaxy, thus making the proposed growth mechanism in the crystal aggregate wrong. Within the present work, the first aim is a theoretical investigation of the homo-epitaxies among the three {10.4}-cleavage, {01.2}-steep and {01.8}-flat rhombohedra of calcite. Accordingly, the specific adhesion energies were calculated between facing crystal forms, unequivocally showing that the {01.2}/{01.8} homo-epitaxy competes with the generation of both {01.2} and {01.8} contact twins. Secondly, the calculation of the specific adhesion energy was extended to consider homo-epitaxy for the {10.4} rhombohedron. The two-dimensional geometric lattice coincidence has been tried for the {00.1} pinacoidal form as well.




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Link between b.c.c.–f.c.c. orientation relationship and austenite morphology in CF8M stainless steel

Slow-cooled CF8M duplex stainless steel is used for critical parts of the primary coolant pipes of nuclear reactors. This steel can endure severe service conditions, but it tends to become more brittle upon very long-term aging (tens of years). Therefore, it is essential to understand its specific microstructure and temporal evolution. As revealed by electron backscatter diffraction (EBSD) analyses, the microstructure consists of millimetre-scale ferritic grains within which austenite lath packets have grown with preferred crystallographic orientations concerning the parent ferritic phase far from the ferrite grain boundaries. In these lath packets where the austenite phase is nucleated, the lath morphology and crystal orientation accommodate the two ferrite orientations. Globally, the Pitsch orientation relationship appears to display the best agreement with the experimental data compared with other classical relationships. The austenite lath packets are parallel plate-shaped laths, characterized by their normal n. A novel methodology is introduced to elucidate the expected relationship between n and the crystallographic orientation given the coarse interfaces, even though n is only partly known from the observation surface, in contrast to the 3D crystal orientations measured by EBSD. The distribution of retrieved normals n is shown to be concentrated over a set of discrete orientations. Assuming that the ferrite and austenite obey the Pitsch orientation relationship, the determined lath normals are close to an invariant direction of the parent phase given by the same orientation relationship.




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VMXm – A sub-micron focus macromolecular crystallography beamline at Diamond Light Source

VMXm joins the suite of operational macromolecular crystallography beamlines at Diamond Light Source. It has been designed to optimize rotation data collections from protein crystals less than 10 µm and down to below 1 µm in size. The beamline has a fully focused beam of 0.3 × 2.3 µm (vertical × horizontal) with a tuneable energy range (6–28 keV) and high flux (1.6 × 1012 photons s−1 at 12.5 keV). The crystals are housed within a vacuum chamber to minimize background scatter from air. Crystals are plunge-cooled on cryo-electron microscopy grids, allowing much of the liquid surrounding the crystals to be removed. These factors improve the signal-to-noise during data collection and the lifetime of the microcrystals can be prolonged by exploiting photoelectron escape. A novel in vacuo sample environment has been designed which also houses a scanning electron microscope to aid with sample visualization. This combination of features at VMXm allows measurements at the physical limits of X-ray crystallography on biomacromolecules to be explored and exploited.




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Travelex partners with NCR Atleos to launch complete ATM technology refresh

Foreign exchange brand Travelex has announced its partners...




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Paymentology partners with Zand Bank to support fintech growth in UAE

Paymentology has entered into a referral partnership with...




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Emerging Technologies and Trends in Identity Verification, KYC, and KYB Report 2024

The inaugural edition of the Emerging Technologies and Trends in Identity Verification (IDV), KYC, and KYB Report 2024 offers a comprehensive overview of the key technology trends and best practices in digital onboarding for consumers and businesses in 2024.




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UAE unveils new payment technology using unique vein patterns

The UAE has announced the introduction of 'Palm ID' technology, a payment solution that utilises the unique vein patterns in individuals' palms for...




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Tintin on acid: Charles Burns ends his dark trilogy with 'Sugar Skull'

Graphic novelist and artist Charles Burns, at the Mohn Broadcast Center, 9/18/2014. He's just completed his trilogy with the new book Sugar Skull.; Credit: John Rabe

Graphic novelist Charles Burns stopped by the Mohn Broadcast Center to talk with Off-Ramp host John Rabe about "Sugar Skull," which completes the trilogy that includes "X'ed Out" and "The Hive."  He's doing a signing tonight (Thurday, Sept. 18) at Skylight Books, 1818 N. Vermont Avenue in Los Feliz.

Why the trilogy? Burns says, "I had finished another book, "Black Hole," which was this very long graphic novel, all in black and white, and I wanted to do something in color, so I conceived a series of books, based on a series of my life, late '70s, the Punk Era. It started that way, and then it turned into something else."

RELATED: Miniaturist Alan Wolfson, or "Honey! I shrunk the strip club!"

The look of Burns' books:

... is drawn from one of his childhood favorites, the Belgian Tintin, who had all sorts of politically incorrect adventures:

"It's called the clear-line school of comics. And it's exactly what it sounds like: very clear lines, but perfectly rendered in color."

If there's more shadow in Burns' books than in Herge's, that's because the lines between good and bad, dark and light, reality and unreality are blurrier in Burns. The trilogy moves back and forth in time and place, including between the normal world and a bizarre world in which a character named Johnny 23 struggles with many of the same issues the protagonist in the real world, Doug, faces... including a pregnant girlfriend.

(From Sugar Skull, by Charles Burns. )

Are they two separate stories or Doug's subconscious world? Burns won't say. "That's for the reader to figure out. I don't really ever clearly explain those things at all, and I like to leave those things open. Interpretation is great, as far as I'm concerned."

Burns turns 59 on Sept. 27. How does he like approaching 60? "As far as the day-to-day landscape, things are more settled in. But as far as my sweltering, swelling, itching brain, that hasn't changed at all unfortunately."




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Premier Bank, Mastercard, Tappy Technologies launch Tap2Pay

Premier Bank, in partnership with



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Lean Technologies raises USD 67.5 million to scale its Pay-by-Bank and Open Banking tools

Saudi Arabia-based fintech infrastructure platform Lean Technologies has raised USD 67.5 million in a Series B funding round to scale its Pay-by-Bank and Open Banking offerings.




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Trick or Treat? Astrogeology explores the Solar System’s Halloween spirit.

The Solar System is full of its own tricks and treats, so discover some of our favorites below.




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Idaho Hydrologic Update, October 2024

October 2024 issue of the Idaho Hydrologic Update from the USGS Idaho Water Science Center.




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Fellows Blog: Meet Science to Action Fellow Emily Nastase!

Emily shares her experience research on Henslow’s sparrow accounting for the future effects of climate change and to develop risk assessment tools to assist managers in the region with meeting their conservation objectives using prescribed fire.




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Volcano Watch — The Art and Science of Geologic Mapping

Geologic mapping has been one of the most fundamental mandates of the U.S. Geological Survey (USGS) since its establishment in 1879. Congress created the USGS to "classify the public lands and examine the geological structure, mineral resources, and products within and outside the national domain."




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Photo and Video Chronology — Getting webcams back online at Mauna Loa summit

Mauna Loa summit webcams have been down for several months due to wind damage at the radio telemetry site. On November 7, 2024, HVO staff visited the site and performed a partial fix that brought the webcams back online.




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Marine Mineral Formations in the Arctic Ocean Challenge Existing Geologic Theories

A new study from USGS describes a previously unknown process of marine mineral formation in the Arctic Ocean, driven by frictional heating along tectonic faults rather than by hydrothermal activity. 




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Metals Co. Expands Into Geological Hydrogen Sector With Department of Energy Grant

This Buy-rated Canadian explorer-developer is working to achieve first mover status in this emerging clean energy space. Find out what all it has done and is doing.




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Metals Co. Expands Into Geological Hydrogen Sector With Department of Energy Grant

This Buy-rated Canadian explorer-developer is working to achieve first mover status in this emerging clean energy space. Find out what all it has done and is doing.




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Government Technology Trends for 2011

What does 2011 hold for technology in government?  This is always hard to predict but we must continually be looking forward, researching the trends, separating the potential break through from the fads, and determining what solid technology in our past is now obsolete.  Investments must be sound to maximize the limited, available dollars.  As we [...]




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Technology Drivers for Trends 2012

Every year about this time I try to predict the technology trends for local government that will influence how we do business in the coming year.  This year, I am doing something a little different.  I am starting with an article that covers some of the changes that we have seen in the past few [...]




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Local Government Technology Trends 2012

Every year about this time I try to predict the technology trends for local government that will influence how we do business in the coming year.  This year, I am doing something a little different.  I started with an article that covered some of the changes we have seen in the past few years that [...]




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New technologies, consumer preferences, sustainability imperatives to shape up future mobility, say experts

Panelists in a discussion on ‘Mobility Megatrends 2050’, highlighted that in the next decade, with electrification, autonomous driving, smart and connected infrastructure, modal diversity, and mobility that is integrated, resilient, shared, and sustainable – powered by disruptive business models, will transform and shape up of the automotive industry. The industry is racing towards a new world, driven by sustainability and changing consumer behaviour, encompassing electric vehicles, autonomous cars, mobility fleet sharing, and always connected.




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Equitable technology reach: The India success story

Through a series of ambitious initiatives and strategic investments, India is actively working to overcome barriers and create a level playing field for technology adoption. From expanding connectivity infrastructure to investing in digital literacy programs, the nation is leaving no stone unturned in its pursuit of equitable access to technology.




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Catawba County developing new GIS Real Estate website to take advantage of new technology, offer enhanced services

Enhancements include an auto-fill feature, the ability to search on a business or landmark name, and links to both Google and Bing maps.




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New Report Proposes Framework To Encourage Fluency With Information Technology

The explosive growth of information technology is having a profound impact on our lives.




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Advances in Biotechnology Show Promise For Improving Army Readiness, Soldier Survival

Recent strides in biotechnology offer the promise of new and innovative applications -- from edible vaccines to protein-based electronics components.




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National Academies Name Biology Teaching Fellows and Mentors

The National Academies have bestowed the title of Education Fellow in the Life Sciences to 42 educators around the country who successfully completed a summer institute aimed at fostering innovative approaches to teaching undergraduate biology.




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Some Pollinator Populations Declining - Improved Monitoring and More Biological Knowledge Needed to Better Assess Their Status

Long-term population trends for some North American pollinators -- bees, birds, bats, and other animals and insects that spread pollen so plant fertilization can occur -- are demonstrably downward, says a new report from the National Research Council.




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Genetically Engineered Crops Benefit Many Farmers, but the Technology Needs Proper Management to Remain Effective

Many U.S. farmers who grow genetically engineered (GE) crops are realizing substantial economic and environmental benefits -- such as lower production costs, fewer pest problems, reduced use of pesticides, and better yields -- compared with conventional crops, says a new report from the National Research Council.




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President Obama Stresses Importance of Science and Technology to the Nations Future in Address at 150th Annual Meeting of the National Academy of Sciences

President Barack Obama reiterated his strong support for science and technology today in a speech to members of the National Academy of Sciences at its 150th annual meeting.




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Analysis Used by Federal Agencies to Set Fuel Economy and Greenhouse Gas Standards for U.S. Cars Was Generally of High Quality - Some Technologies and Issues Should Be Re-examined

The analysis used by federal agencies to set standards for fuel economy and greenhouse gas emissions for new U.S. light-duty vehicles -- passenger cars and light trucks -- from 2017 to 2025 was thorough and of high caliber overall, says a new report from the National Research Council.




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Effective Monitoring to Evaluate Ecological Restoration in the Gulf of Mexico – New Report

To improve and ensure the efficacy of restoration efforts in the Gulf of Mexico following Deepwater Horizon – the largest oil spill in U.S. history – a new report from the National Academies of Sciences, Engineering, and Medicine recommends a set of best practices for monitoring and evaluating ecological restoration activities.




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U.S. Should Act to Support Innovation in Increasingly Clean Electric Power Technologies

A new report from the National Academies of Sciences, Engineering, and Medicine urges Congress, federal and state agencies, and regulatory institutions to significantly increase their support for innovation for what the report’s study committee calls “increasingly clean” electric power technologies – nuclear power, carbon capture and storage, and renewables such as solar and wind. Some of these technologies have seen recent cost and price declines and are cost-competitive in certain locations.




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USAID Should Speed Application of Science, Technology, and Innovation to Global Development Challenges

The U.S. Agency for International Development should speed its transformation into a global leader and catalyst in applying science, technology and innovation to the challenges facing developing countries, says a new report from the National Academies of Sciences, Engineering, and Medicine.




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New Report Examines Role of Engineering Technology, Calls for Increased Awareness of Field of Study and Employment

While workers in the engineering technology (ET) field play an important role in supporting U.S. technical infrastructure and the country’s capacity for innovation, there is little awareness of ET as a field of study or category of employment in the U.S., says a new report from the National Academy of Engineering.




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Opening Remarks from Bruce Darling, Executive Officer, National Academy of Sciences and National Research Council Report Release Event for Preparing for Future Products of Biotechnology

Good morning. Welcome to the release of the report Preparing for Future Products of Biotechnology, from the National Academies of Sciences, Engineering, and Medicine.




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Federal Regulatory Agencies Need to Prepare for Greater Quantity and Range of Biotechnology Products

A profusion of biotechnology products is expected over the next five to 10 years, and the number and diversity of new products has the potential to overwhelm the U.S. regulatory system, says a new report from the National Academies of Sciences, Engineering, and Medicine.




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New Report Outlines Research Agenda to Address Impact of Technology on Workforce

Federal agencies or other organizations responsible for sponsoring research or collecting data on technology and the workforce should establish a multidisciplinary research program that addresses unanswered questions related to the impact of changing technology on the nature of work and U.S. national economy, says a new report by the National Academies of Sciences, Engineering, and Medicine.




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U.S. DRIVE Partnership Makes Significant Technology Advancements for Light-Duty Vehicles

The U.S. DRIVE Partnership – a government-industry partnership that fosters the development of precompetitive and innovative technologies for clean and efficient light-duty vehicles – has made significant progress in many technical areas including advanced combustion technologies, durability and cost of hydrogen fuel cells, and electric drive systems such as motors, power electronics, and batteries, says a new report by the National Academies of Sciences, Engineering, and Medicine.




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New Report Examines How Assistive Technologies Can Enhance Work Participation for People With Disabilities

Assistive products and technologies – such as wheelchairs, upper-limb prostheses, and hearing and speech devices – hold promise for partially or fully mitigating the effects of impairments and enabling people with disabilities to work, but in some cases environmental and personal factors create additional barriers to employment, says a new report from the National Academies of Sciences, Engineering, and Medicine.




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New Report Proposes Framework to Identify Vulnerabilities Posed by Synthetic Biology

Given the possible security vulnerabilities related to developments in synthetic biology – a field that uses technologies to modify or create organisms or biological components – a new report by the National Academies of Sciences, Engineering, and Medicine proposes a framework to identify and prioritize potential areas of concern associated with the field.




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U.S. Has Lost Its Dominance in Highly Intense, Ultrafast Laser Technology to Europe and Asia

The U.S. is losing ground in a second laser revolution of highly intense, ultrafast lasers that have broad applications in manufacturing, medicine, and national security, says a new report from the National Academies of Sciences, Engineering, and Medicine.




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If Misused, Synthetic Biology Could Expand the Possibility of Creating New Weapons - DOD Should Continue to Monitor Advances in the Field, New Report Says

Synthetic biology expands the possibilities for creating new weapons — including making existing bacteria and viruses more harmful — while decreasing the time required to engineer such organisms, concludes a new report by the National Academies of Sciences, Engineering, and Medicine.




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NASA Should Expand the Search for Life in the Universe and Make Astrobiology an Integral Part of its Missions, Says New Report

To advance the search for life in the universe, NASA should support research on a broader range of biosignatures and environments, and incorporate the field of astrobiology into all stages of future exploratory missions, says a new congressionally mandated report from the National Academies of Sciences, Engineering, and Medicine.




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‘Carbon Utilization’ Technologies Could Reduce Emissions by Turning Greenhouse Gases Into Useful Products - New Report Identifies R&D to Make Technologies More Commercially Viable

A new report from the National Academies of Sciences, Engineering, and Medicine outlines a research agenda for improving the commercial viability of technologies that turn greenhouse gases from the burning of fossil fuels into useful products such as fuels, construction materials, and chemicals.