emo Netflix defends its decision to remove Palestinian films from platform By www.jpost.com Published On :: Sun, 27 Oct 2024 09:54:40 GMT Netflix explained, "We launched this licensed collection of films in 2021 for three years. Those licenses have now expired." Full Article Palestinians film netflix antisemitism
emo Democrats Preparing for Massive Losses By theblacksphere.net Published On :: Tue, 05 Nov 2024 14:25:41 +0000 The following article, Democrats Preparing for Massive Losses, was first published on The Black Sphere. Trump goes into Alvin Braggs' and Letitia James' backyard, and takes a piss. And Chuck Schumer, Hillary Clinton, Kirsten Hillebrand... Continue reading Democrats Preparing for Massive Losses ... Full Article Featured Human Interest
emo Not Looking Good for Kamala Harris or Democrats in General By theblacksphere.net Published On :: Wed, 06 Nov 2024 03:26:25 +0000 The following article, Not Looking Good for Kamala Harris or Democrats in General, was first published on The Black Sphere. Republicans have picked up a Senate seat, and will likely add 2 more. But the best news will come in just a few hours... Continue reading Not Looking Good for Kamala Harris or Democrats in General ... Full Article Featured News
emo 7 Bad Signs for Democrats: A Satirical Take on America’s Return to Normalcy By theblacksphere.net Published On :: Sat, 09 Nov 2024 04:24:17 +0000 The following article, 7 Bad Signs for Democrats: A Satirical Take on America’s Return to Normalcy, was first published on The Black Sphere. Yes, the Democrats got shellacked in the last election. And while that’s a breath of fresh air, it’s hardly the only sign that the political winds are changing. Continue reading 7 Bad Signs for Democrats: A Satirical Take on America’s Return to Normalcy ... Full Article Featured News
emo Election Post-Mortem and Democrat Despair Part I By theblacksphere.net Published On :: Sun, 10 Nov 2024 12:16:53 +0000 The following article, Election Post-Mortem and Democrat Despair Part I, was first published on The Black Sphere. Donald Trump went John Wyck on Democrats, like they killed his dog. And Trump’s decimation of the Party of Lynching has major implications for Democrats. At this point, if establishment Republicans don’t jump on the Trump train, then they deserve to get steamrolled with the Democrats. Because, Trump signaled without a doubt that he and … Continue reading Election Post-Mortem and Democrat Despair Part I ... Full Article Featured
emo Election Post-Mortem and Democrat Despair Part II By theblacksphere.net Published On :: Sun, 10 Nov 2024 23:03:09 +0000 The following article, Election Post-Mortem and Democrat Despair Part II, was first published on The Black Sphere. Donald Trump made it clear in no uncertain terms that he plans to dismantle the Deep State. And it’s rants like this from Joe Scarborough that explain the new and improved version of Trump. Watch as Scarborough goes on a tirade about Trump right after learning of Trump’s victory. In his defense, almost every Leftist … Continue reading Election Post-Mortem and Democrat Despair Part II ... Full Article Featured News
emo The Democrats’ Enormous Mistake By theblacksphere.net Published On :: Mon, 11 Nov 2024 11:36:45 +0000 The following article, The Democrats’ Enormous Mistake, was first published on The Black Sphere. In the continuing search for blame for what may forever be known by me as Democrats' Most Awesome 2024 Ass-Kicking... Continue reading The Democrats’ Enormous Mistake ... Full Article Featured Politics
emo Democrats May Soon Need Another Beatdown By theblacksphere.net Published On :: Tue, 12 Nov 2024 16:44:37 +0000 The following article, Democrats May Soon Need Another Beatdown, was first published on The Black Sphere. If you think Democrats are soul-searching the bona fide ass-kicking they took in the 2024 election, then you don't know Democrats. Continue reading Democrats May Soon Need Another Beatdown ... Full Article Featured Human Interest
emo Mystery tremors were from massive nine-day tsunami By www.bbc.com Published On :: Thu, 12 Sep 2024 18:17:55 GMT Scientists launched an investigation after being baffled by seismic signals picked up across the world Full Article
emo Democrats' bet on a generation of liberal voters has backfired badly By www.bbc.com Published On :: Sun, 10 Nov 2024 06:35:38 GMT Latino, black and younger voters once helped Obama win power - Trump made gains with all three groups this election. Why? Full Article
emo Silence falls for Armistice Day commemorations By www.bbc.com Published On :: Mon, 11 Nov 2024 12:12:07 GMT Those who died in two world wars and other conflicts are remembered on Armistice Day across the UK and internationally. Full Article
emo How To Remove Rust From A Gun Barrel? [Updated 2023] By patriotoutdoornews.com Published On :: Mon, 13 Nov 2023 15:40:46 +0000 Gun barrels are made of metal, and like any other metal object, they just love to get rusty. After you use the gun for a while, they start acting like a toddler who needs nonstop attention. If you leave them unattended for a while, you’ll come back to see that the shiny barrel is cluttered […] The post How To Remove Rust From A Gun Barrel? [Updated 2023] appeared first on Patriot Outdoor News. Full Article Gear & Accessories Gun Barrel Rust Removing
emo London's new lord mayor: 'It's not just ceremonial' By www.bbc.com Published On :: Sat, 09 Nov 2024 08:06:41 GMT Alastair King is the 696th person to be appointed as the lord mayor of the City of London. Full Article
emo “If the Democrats could fight wars as well as they execute Election Fraud, we would have obliterated all of our many enemies throughout the World, and would have nothing to worry about!” ~ Donald J. Trump By powderedwigsociety.com Published On :: Sun, 29 Aug 2021 14:34:49 +0000 Touché! .@POTUS45: "If the Democrats could fight wars as well as they execute Election Fraud, we would have obliterated all of our many enemies throughout the World, and would have nothing to worry about!" pic.twitter.com/GpMO46zrNQ — Natalie Harp (@NatalieJHarp) August […] The post “If the Democrats could fight wars as well as they execute Election Fraud, we would have obliterated all of our many enemies throughout the World, and would have nothing to worry about!” ~ Donald J. Trump appeared first on Powdered Wig Society. Full Article 2020 presidential election 2020 election fraud afghanistan donald trump
emo Typical Democrat: Harris Raised One BILLION for her Campaign… Ended it $20 Million In Debt By lidblog.com Published On :: Sat, 09 Nov 2024 00:36:46 +0000 In what seems like the work of a typical Democrat, Kama Harris raised one billion dollars for her campaign… and finished it $20 million in debt. Imagine what she would have done to the United States which would have started out bankrupt if Harris had entered the White House. How do you have an actual […] The post Typical Democrat: Harris Raised One BILLION for her Campaign… Ended it $20 Million In Debt appeared first on The Lid. Full Article Election 2024
emo Lunatic Democrat Murders His Wife and Kids Over His Hate for Donald Trump By lidblog.com Published On :: Tue, 12 Nov 2024 02:10:15 +0000 This is how mentally deranged liberals are… a Democrat in Minnesota was so filled with rage that Donald Trump won the election last week that he murdered his own wife and kids and then committed suicide to prevent them all from having to live during the next Trump presidency. Notice how you never heard any […] The post Lunatic Democrat Murders His Wife and Kids Over His Hate for Donald Trump appeared first on The Lid. Full Article Election 2024
emo Donald Trump’s New Border Czar Means Business… and Democrats are Furious By lidblog.com Published On :: Tue, 12 Nov 2024 02:15:52 +0000 Donald Trump has riled the left-wing, anti-American Democrats once again, this time by naming Biden critic and immigration expert Thomas D. Homan as his “border czar.” Homan is an excellent choice for this, certainly. No one knows more about fixing border policy better than Homan who ha spent the last 5 years bedeviling the left […] The post Donald Trump’s New Border Czar Means Business… and Democrats are Furious appeared first on The Lid. Full Article Election 2024 Immigration
emo News24 | OPINION | Lisa Otto: Isolationism vs hegemony... and the contradictions of Trump's America By www.news24.com Published On :: Wednesday Nov 13 2024 06:18:13 America and Americans must decide what the US' place in the world is and then accept what comes with that, writes Lisa Otto. Full Article
emo News24 | Teargas, smoke in Maputo as police crack down on demonstrations By www.news24.com Published On :: Thursday Nov 07 2024 12:49:50 In some parts of the capital, protesters negotiated with police. In other parts there was no talking. Full Article
emo News24 | Senzo Meyiwa murder accused removed from C-Max centre By www.news24.com Published On :: Wednesday Nov 13 2024 07:39:54 It was out of fear for his mental health and to avoid the perpetuation of an ongoing wrong that a judge ordered that one of the five men charged with the murder of soccer star Senzo Meyiwa be removed from solitary confinement. Full Article
emo Lunatic Democrat Murders Wife and Kids, Commits Suicide Over His Hate for Donald Trump By conservativefiringline.com Published On :: Tue, 12 Nov 2024 16:15:34 +0000 The following article, Lunatic Democrat Murders Wife and Kids, Commits Suicide Over His Hate for Donald Trump, was first published on Conservative Firing Line. This is how mentally deranged liberals are… a Democrat in Minnesota was so filled with rage that Donald Trump won the election last week that he murdered his own wife and kids and then committed suicide to prevent them all from having to live during the next Trump presidency. Notice how you never heard any … Continue reading Lunatic Democrat Murders Wife and Kids, Commits Suicide Over His Hate for Donald Trump ... Full Article Crime Politics Anthony Nephew Biden children garbage kill mental illness partner Trump wife
emo ‘Burn The System Down’: Democrats Now Face Charges They Are The Ones Trying To Destroy Democracy By conservativefiringline.com Published On :: Wed, 13 Nov 2024 06:39:47 +0000 The following article, ‘Burn The System Down’: Democrats Now Face Charges They Are The Ones Trying To Destroy Democracy, was first published on Conservative Firing Line. Protecting democracy was a catch phrase that Democrats have used for years to explain their hatred of now President-elect Donald Trump. He was, after all, they said, a “Hitler.” He would be a dictator. He would use the military against his political opponents, jailing them and worse. The only salvation for America’s “democracy” would be … Continue reading ‘Burn The System Down’: Democrats Now Face Charges They Are The Ones Trying To Destroy Democracy ... Full Article Politics Biden burn Democracy democrats Trump Turley
emo EPIC: When AI Swaps ‘Democracy’ For THIS Word, Journos Start Telling The Truth By clashdaily.com Published On :: Wed, 06 Nov 2024 06:35:52 +0000 Shamelessly, Big Media doesn't even try to hide their Democrat preferences anymore. They are happy to lie. But even the lies they tell can point indirectly to the truth. The post EPIC: When AI Swaps ‘Democracy’ For THIS Word, Journos Start Telling The Truth appeared first on Clash Daily. Full Article Videos
emo Deaf Democrats By clashdaily.com Published On :: Fri, 08 Nov 2024 02:51:01 +0000 Democrats don't listen. The post Deaf Democrats appeared first on Clash Daily. Full Article Opinion
emo Why Democrats Lost the 2024 Elections By clashdaily.com Published On :: Fri, 08 Nov 2024 03:00:04 +0000 If Democrat politicians want our votes in the future then it would be nice if they said they were sorry for what they have done in the past. We don’t have far to look. Let’s see at why they lost and how they can win. The post Why Democrats Lost the 2024 Elections appeared first on Clash Daily. Full Article Opinion
emo We Dodged A Demonic Political Bullet By clashdaily.com Published On :: Fri, 08 Nov 2024 10:30:15 +0000 Take a good look at the people melting down on social media because their team didn't win an election. That's what it looks likes when your politics becomes your faith. The post We Dodged A Demonic Political Bullet appeared first on Clash Daily. Full Article Doug's Columns
emo Demonic & Damned to Ridiculously Redeemed! (Eph.2:1-10) By clashdaily.com Published On :: Mon, 11 Nov 2024 10:45:49 +0000 We all know that *one* guy who acts like he's doing the world a favor just by showing up. The post Demonic & Damned to Ridiculously Redeemed! (Eph.2:1-10) appeared first on Clash Daily. Full Article Doug's Columns
emo Democrats Have Another Problem to Worry About as Man Who Helped Trump Win Has Eyes on Blue State By www.westernjournal.com Published On :: Tue, 12 Nov 2024 00:06:25 +0000 Conservative activist Scott Presler, whose organization Early Vote Action helped swing Pennsylvania to the right in the general election, announced Monday he will be working to do the same in […] The post Democrats Have Another Problem to Worry About as Man Who Helped Trump Win Has Eyes on Blue State appeared first on The Western Journal. Full Article News 2024 election New Jersey Pennsylvania Republicans Voting
emo Democratic Politician Crashes Her Car While Allegedly Drunk, Hurls Vile Insults at Responding Officer: Police Report By www.westernjournal.com Published On :: Tue, 12 Nov 2024 21:35:14 +0000 A Chicago-area Democrat hurled an insult at police Sunday after being charged with drunken driving, according to local news reports. Samantha Steele represents the Second District on the Cook County […] The post Democratic Politician Crashes Her Car While Allegedly Drunk, Hurls Vile Insults at Responding Officer: Police Report appeared first on The Western Journal. Full Article News Alcohol Chicago Democrats Investigation Police U.S. News
emo Sport | Key dying minutes of Tests: Boks have been demons, England disasters By www.news24.com Published On :: Tuesday Nov 12 2024 10:06:14 In his latest Rucking with Rob newsletter, Rob Houwing argues that for England to have any hopes of beating the Springboks, they'll have to be on the ball in the final stages. Full Article
emo Digital politics threatens democracy and must change By www.chathamhouse.org Published On :: Fri, 13 Jan 2023 16:08:06 +0000 Digital politics threatens democracy and must change Expert comment NCapeling 13 January 2023 Violence in Brazil has again brought into focus the need for technology platforms to prioritize social responsibility to help prevent anti-democratic action. Immediately following the violent storming of Brazil’s congressional building, supreme court, and presidential palace, comparisons to the infamous events of 6 January 2021 in the US came quickly and easily – and with good reason. Both Donald Trump and Jair Bolsonaro built a fervent – albeit inchoate – political base of grievance which included Christian evangelicals, gun enthusiasts, and the disenfranchised. Both cynically spread doubts about the election victories of their successors without evidence. And it has been claimed both incited their supporters to overturn these results. But this list of similarities extend beyond mere politics. The attack on democracy in Brazil was recorded, amplified, coordinated, and funded by exactly the same technologies used by the protesters in the US on 6 January. In Brazil, the main engines of misinformation and insurrection planning were Telegram, Facebook, and WhatsApp, although some election-denying diehards also shifted to Twitter. Tactical use of social media to mobilize and fundraise When Bolsonaro was originally elected in 2018, these platforms were already a medium for promoting disinformation, conspiracy, and fear. But in the lead-up to the violence of 8 January, they became a means by which ‘Bolsonaristas’ raised funding for an attack and mobilized supporters to come to Brasilia – advertising the availability of buses and even free food for marchers – using the Festa da Selma hashtag. Well-intentioned regulation will fall short so long as it is narrowly focused on a never-ending game of ‘content whack-a-mole’ and is hamstrung by powerful voices To avoid detection by authorities, organizers used a simple switch of the letter ‘v’ to ‘m’ so that Selva, meaning ‘jungle’, became Selma – which means ‘party in the jungle’ to those in the know. But the authorities in the Brasilia state of Distrito Federal appeared to care little about preventing the attack anyway. Civil society organizations have warned for decades that policies made in a Silicon Valley boardroom fit poorly to the realities of countries such as Brazil, Somalia, or Myanmar. Content moderation is a near-impossible task at the scale demanded by platforms as vast as those operated by big tech, and automated solutions are far from being a silver bullet as they create as many problems as they solve. Extremism has found an unfettered enabler on mainstream platforms, and an increasingly sprawling network of alternative tech has made such voices resilient to challenge. Platforms such as Gettr, Gab, and Telegram have become go-to platforms for extremist networks when fringe voices find themselves blocked by mainstream platforms. Telegram has come under significant scrutiny for the role it played in the storming of Congress in Brasilia but any number of tools and platforms – each with millions of users – could have achieved similar results. The sordid events in Brasilia are just the latest chapter in digital extremism and conspiracy- mongering which often erupts into anti-democratic violence, and should serve as a reminder of the urgent risks of disinformation and the role digital technology plays in inciting, coordinating, fundraising, and amplifying such events. But the checks on the power of private social media companies are still few and splintered. In Brazil, Supreme Court Justice Alexandre de Moraes, head of the elections tribunal, attempted to crack down on social media, banning users for spreading misinformation in a move which sparked complaints of censorship from Bolsonaro supporters. Following the elections, Moraes has advocated for greater judicial authority to regulate social media – but the justice is only one person and his impartiality is already being challenged given his aggressive pursuit of Bolsonaro supporters posting inflammatory messages. And one justice doing it alone only inflames an already volatile situation. Effective regulation of social media requires an independent body vested with multiparty support and operating under clearly-defined rules and authority. A global movement to find solutions Brazil is not alone in an increasingly frantic search for checks on digital power as dozens of regulatory regimes are springing up around the world. All are premised on platforms being responsible for their own fiefdoms, but few tackle the threat posed to democracy by unchecked corporate power over digital commons. Telegram has come under significant scrutiny for the role it played in the storming of Congress in Brasilia but any number of tools and platforms – each with millions of users – could have achieved similar results Well-intentioned regulation will fall short so long as it is narrowly focused on a never-ending game of ‘content whack-a-mole’ and is hamstrung by powerful voices who see any rules as an attack on freedoms of expression or corporate decision-making. Democracies are traditionally cautious in managing speech, in separating good information from bad information, or in defining acceptable politics. The steady watering down of the UK’s Online Safety Bill, now so thin on disinformation as to be essentially homeopathic, shows that democratic regulation is willing to go only so far in tackling challenges posed by digital politics. Full Article
emo The Democratic Republic of the Congo’s foreign policy priorities By www.chathamhouse.org Published On :: Fri, 18 Oct 2024 19:42:13 +0000 The Democratic Republic of the Congo’s foreign policy priorities 29 October 2024 — 1:00PM TO 2:00PM Anonymous (not verified) 18 October 2024 Chatham House and Online The DRC’s Minister of Foreign Affairs discusses the country’s approach to regional stability and the role of international partnerships in securing economic prosperity. At this event, HE Thérèse Kayikwamba Wagner, Minister of Foreign Affairs of the DRC, will discuss DRC’s regional diplomatic priorities and international agency, including its ambitions around global critical mineral supply chains. The Democratic Republic of the Congo (DRC) faces foreign policy tests as it manages a complex security crisis and regional tensions, alongside global economic and trade partnerships.Entrenched conflict in eastern DRC continues to have wider regional implications. Troops from the Southern African Development Community (SADC) deployment are tackling a growing mandate as the UN’s peacekeeping mission prepares to withdraw. Tensions with Rwanda also remain high despite mediation efforts under the Luanda process.As a major producer of critical minerals for the global energy transition, the DRC seeks to benefit from growing international demand without sacrificing value addition. New and existing frameworks with international partners will remain key to this vision, including for the development of regional infrastructure networks such as the Lobito Corridor.At this event, HE Thérèse Kayikwamba Wagner, Minister of Foreign Affairs of the DRC, will discuss the DRC’s regional diplomacy and international agency, including the country’s positioning in global critical mineral supply chains.Please note that in-person attendance for this event will be balloted. Guests looking to join in-person must register interest in joining. Full confirmations to successful applicants will be sent on Friday 25 October. Full Article
emo Trust in US democracy is at stake in this election By www.chathamhouse.org Published On :: Wed, 23 Oct 2024 08:50:44 +0000 Trust in US democracy is at stake in this election Expert comment rgold.drupal 23 October 2024 A narrow win for either side could make things worse. The rest of the world should be prepared for a disruptive transition. With less than two weeks to election day in the United States, polls suggest the gap between Harris and Trump has narrowed even further in the swing states where the election will be decided. A fierce contest has ensued as each campaign seeks to drive voter turnout. In such a tight race, this will be decisive. But in an election that many Americans perceive as being existential for the country’s future, a narrow victory by either side will heighten the risk of a contested election. Related content The US election will take place in a polluted information space Many Americans are pessimistic about the ability of democracy to deliver a reliable result. Two thirds of Republican voters continue to believe that the 2020 election was stolen from Donald Trump and for many of these, Biden’s inauguration may have demonstrated the weakness, not the strength, of US democracy. This environment has contributed to widespread anticipation of a contested election, as well as preparations for the possibility of a delayed result. Political disinformation and deep polarization across the country could mean that a narrow victory leads to more legal challenges and even political violence. 6 January, the anniversary of the assault on the US Capitol and the day Congress will certify the Electoral College has been designated a National Special Security Event. The issue of how quickly and reliably votes are certified has become an arena for argument. In the 2022 midterms, 22 county officials in key states voted to delay certification. Democrats have noted with alarm that this year, around 70 pro-Trump election ‘conspiracists’ are positioned to review the results in swing states.Those nervous about the possibility of a narrow vote being overturned will be watching to see if Republicans maintain or increase their majority in the House of Representatives, since the newly elected House will shape who holds the gavel when a joint session of Congress returns to certify the US presidential election result. Republican control of the House could give the former president more ability to influence this vote. A resilient democracyBut there should be room for optimism. Democracy in America has been remarkably resilient. In 2016, Democrats swallowed their unease with Donald Trump. Despite rumours of Russian interference in the election, they accepted the election result. If fears of another contested election materialise, it will be the third time in recent history that the US has struggled to confirm a result. Four years later, the US was far more polarized, trust in institutions had decreased, and challenges to the elections reflected this. The Trump campaign filed more than 60 lawsuits in 9 states challenging election processes and voter certification. In every single case, courts confirmed the results. After the 6 January insurrection on the US Capitol, Congress returned to its chambers and, on the very same day, confirmed the electoral results. If fears of another contested election materialise, it will be the third time in recent history that the US has struggled to confirm a result. In 2000, the Supreme Court intervened to decide on a recount in Florida, effectively delivering victory to George W Bush – a memory alongside the 6 January Capitol riots contributing to America’s anxiety ahead of polling day. International responseThe US’s partners and rivals may be ill-prepared for a contested election. If Trump or Harris, or both, claim victory before it is clearly settled, world leaders will need to decide how to react. Diplomatic protocol may dictate that leaders wait until the US confirms the results through official channels but there are concerns that not everyone will play by these rules. Full Article
emo Unfulfilled Ambitions: the State of Democracy in Africa By f1.media.brightcove.com Published On :: Mon, 04 Jun 2018 00:00:00 +0100 Full Article
emo Cyber Security Series: Securing Elections and Reclaiming Democratic Processes By f1.media.brightcove.com Published On :: Mon, 03 Sep 2018 00:00:00 +0100 Full Article
emo The Future of Democracy in Asia By f1.media.brightcove.com Published On :: Mon, 01 Oct 2018 00:00:00 +0100 Full Article
emo Martin Wight Memorial Lecture: The Future of Think-Tanks By f1.media.brightcove.com Published On :: Wed, 21 Nov 2018 00:00:00 +0000 Full Article
emo Is Technology Destroying Democracy? By f1.media.brightcove.com Published On :: Thu, 07 Feb 2019 00:00:00 +0000 Full Article
emo Stacey Abrams: Democracy and the Politics of Identity By f1.media.brightcove.com Published On :: Wed, 06 Mar 2019 00:00:00 +0000 Full Article
emo What Brexit Satisfies the Democratic Will of the People? By f1.media.brightcove.com Published On :: Tue, 02 Apr 2019 00:00:00 +0100 Full Article
emo Are ‘Digital Parties’ the Future of Democracy in Europe? By f1.media.brightcove.com Published On :: Tue, 07 May 2019 00:00:00 +0100 Full Article
emo Direct Democracy: Participation Without Populism? By f1.media.brightcove.com Published On :: Mon, 24 Jun 2019 00:00:00 +0100 Full Article
emo Iran, Islam and Democracy: The Politics of Managing Change 20 Years On By f1.media.brightcove.com Published On :: Tue, 02 Jul 2019 00:00:00 +0100 Full Article
emo How Democratic Is the EU? By f1.media.brightcove.com Published On :: Wed, 25 Sep 2019 00:00:00 +0100 Full Article
emo 20 Years On: Removal of the Ban on LGBTIQ+ Personnel Serving in the UK Armed Forces By f1.media.brightcove.com Published On :: Wed, 29 Jan 2020 00:00:00 +0000 Full Article
emo The State of Democracy in Turkey By f1.media.brightcove.com Published On :: Mon, 03 Feb 2020 00:00:00 +0000 Full Article
emo Chatham House Primer: Democratic Socialism By f1.media.brightcove.com Published On :: Thu, 13 Feb 2020 00:00:00 +0000 Full Article
emo Apolipoprotein-mediated removal of cellular cholesterol and phospholipids By www.jlr.org Published On :: 1996-12-01 JF OramDec 1, 1996; 37:2473-2491Reviews Full Article
emo Chatham House Commission on Democracy and Technology in Europe By www.chathamhouse.org Published On :: Thu, 25 Jul 2019 14:47:34 +0000 Chatham House Commission on Democracy and Technology in Europe News Release sysadmin 25 July 2019 Our project on Democracy and Technology in Europe is now entering its final phase. Now we want your help in shaping the final report. Full Article
emo MicroRNA-98 reduces nerve growth factor expression in nicotine-induced airway remodeling [Gene Regulation] By www.jbc.org Published On :: 2020-12-25T00:06:30-08:00 Evolving evidence suggests that nicotine may contribute to impaired asthma control by stimulating expression of nerve growth factor (NGF), a neurotrophin associated with airway remodeling and airway hyperresponsiveness. We explored the hypothesis that nicotine increases NGF by reducing lung fibroblast (LF) microRNA-98 (miR-98) and PPARγ levels, thus promoting airway remodeling. Levels of NGF, miR-98, PPARγ, fibronectin 1 (FN1), endothelin-1 (EDN1, herein referred to as ET-1), and collagen (COL1A1 and COL3A1) were measured in human LFs isolated from smoking donors, in mouse primary LFs exposed to nicotine (50 μg/ml), and in whole lung homogenates from mice chronically exposed to nicotine (100 μg/ml) in the drinking water. In selected studies, these pathways were manipulated in LFs with miR-98 inhibitor (anti-miR-98), miR-98 overexpression (miR-98 mimic), or the PPARγ agonist rosiglitazone. Compared with unexposed controls, nicotine increased NGF, FN1, ET-1, COL1A1, and COL3A1 expression in human and mouse LFs and mouse lung homogenates. In contrast, nicotine reduced miR-98 levels in LFs in vitro and in lung homogenates in vivo. Treatment with anti-miR-98 alone was sufficient to recapitulate increases in NGF, FN1, and ET-1, whereas treatment with a miR-98 mimic significantly suppressed luciferase expression in cells transfected with a luciferase reporter linked to the putative seed sequence in the NGF 3'UTR and also abrogated nicotine-induced increases in NGF, FN1, and ET-1 in LFs. Similarly, rosiglitazone increased miR-98 and reversed nicotine-induced increases in NGF, FN1, and ET-1. Taken together, these findings demonstrate that nicotine-induced increases in NGF and other markers of airway remodeling are negatively regulated by miR-98. Full Article