You know, until the lawsuit. ~Not-So-Handy Andy

Princeton University researchers have discovered that the bacteria behind the life-threatening disease cholera initiates infection by coordinating a wave of mass shapeshifting that allows them to more effectively penetrate their victims' intestines. The researchers also identified the protein that allows Vibrio cholerae to morph, and found that it's activated through quorum sensing. The findings could lead to new treatments for cholera that target the bacteria's ability to change shape or penetrate the gut.

Researchers at Princeton and Rutgers universities have found that the hepatitis E virus — an emerging liver virus historically found in developing countries but now on the rise in Europe — uses a technique to spread infection that scientists could in fact exploit to treat the disease.

DALLAS – (April 23, 2020)  The U.S. Environmental Protection Agency (EPA) announced the 2019 winners of the national WasteWise awards.

Some papers report the PM will unveil a new coronavirus warning system in his speech this evening.

A bodyguard of Eastern Cape Social Development MEC Siphokazi Mani-Lusithi has been arrested with four others by the Green Scorpions for illegal hunting on a private farm outside East London, as well as for breaking Covid-19 lockdown regulations.

Antibodies are widely used as cancer therapeutics, but their current use is limited by the low number of antigens restricted to cancer cells. A receptor tyrosine kinase, receptor tyrosine kinase-like orphan receptor 2 (ROR2), is normally expressed only during embryogenesis and is tightly down-regulated in postnatal healthy tissues. However, it is up-regulated in a diverse set of hematologic and solid malignancies, thus ROR2 represents a candidate antigen for antibody-based cancer therapy. Here we describe the affinity maturation and humanization of a rabbit mAb that binds human and mouse ROR2 but not human ROR1 or other human cell-surface antigens. Co-crystallization of the parental rabbit mAb in complex with the human ROR2 kringle domain (hROR2-Kr) guided affinity maturation by heavy-chain complementarity-determining region 3 (HCDR3)-focused mutagenesis and selection. The affinity-matured rabbit mAb was then humanized by complementarity-determining region (CDR) grafting and framework fine tuning and again co-crystallized with hROR2-Kr. We show that the affinity-matured and humanized mAb retains strong affinity and specificity to ROR2 and, following conversion to a T cell–engaging bispecific antibody, has potent cytotoxicity toward ROR2-expressing cells. We anticipate that this humanized affinity-matured mAb will find application for antibody-based cancer therapy of ROR2-expressing neoplasms.

Lens proteins become increasingly cross-linked through nondisulfide linkages during aging and cataract formation. One mechanism that has been implicated in this cross-linking is glycation through formation of advanced glycation end products (AGEs). Here, we found an age-associated increase in stiffness in human lenses that was directly correlated with levels of protein–cross-linking AGEs. α-Crystallin in the lens binds to other proteins and prevents their denaturation and aggregation through its chaperone-like activity. Using a FRET-based assay, we examined the stability of the αA-crystallin–γD-crystallin complex for up to 12 days and observed that this complex is stable in PBS and upon incubation with human lens–epithelial cell lysate or lens homogenate. Addition of 2 mm ATP to the lysate or homogenate did not decrease the stability of the complex. We also generated complexes of human αA-crystallin or αB-crystallin with alcohol dehydrogenase or citrate synthase by applying thermal stress. Upon glycation under physiological conditions, the chaperone–client complexes underwent greater extents of cross-linking than did uncomplexed protein mixtures. LC-MS/MS analyses revealed that the levels of cross-linking AGEs were significantly higher in the glycated chaperone–client complexes than in glycated but uncomplexed protein mixtures. Mouse lenses subjected to thermal stress followed by glycation lost resilience more extensively than lenses subjected to thermal stress or glycation alone, and this loss was accompanied by higher protein cross-linking and higher cross-linking AGE levels. These results uncover a protein cross-linking mechanism in the lens and suggest that AGE-mediated cross-linking of α-crystallin–client complexes could contribute to lens aging and presbyopia.

European dominance in U.S. immigration flows has decreased significantly since World War II, a result of economic, demographic, and policy trends on both sides of the Atlantic. Today, migration from European Union Member States to the United States, while small, is characterized by a substantial numbers of European scientists, professionals, and businesspeople.

Correctional Service of Canada says an inmate, who escaped from an Edmonton facility in 2018, was arrested by police in Saskatoon on Saturday morning.

The Nova Scotia government is going ahead with its annual lottery for moose hunting licences after the latest survey found Cape Breton moose numbers have rebounded from a low of 1,300 last year.

Santa Cruz De Tenerife, Spain :: Speakers talk about the destructive power of negative words at a ladies' conference on board Logos Hope.

Saudi Arabia has been burning through its currency reserves amid the fall in oil prices. Is the economy heading toward peril or will reforms save the day?

With the fishing season going poorly in Kapembwa, Zambia, the people want to know why, and to find the answers, they’ve called in a witch doctor. 

The Rs 75,000-crore paper industry, which has resumed production after relaxations were issued effective from April 15, may have a negative growth in the first quarter of this fiscal as demand lost in this lockdown cannot be compensated entirely.

Kerala Chief Minister Pinarayi Vijayan remembered his mother on Mother's Day and said that the courage and determination with which his mother faced the life, taught him valuable lessons and laid the...

According to Dun & Bradstreet's latest Economy Forecast, the changes in consumer behaviour will determine the segments that are likely to flourish after the pandemic is brought under control.

With the latest cut in prices, a 14.2 kg non-subsidised LPG cylinder would cost Rs 581.50, instead of Rs 744 with effect from today.

This Metasploit module exploits sendmail's well-known historical debug mode to escape to a shell and execute commands in the SMTP RCPT TO command. This vulnerability was exploited by the Morris worm in 1988-11-02. Cliff Stoll reports on the worm in the epilogue of The Cuckoo's Egg. Currently only cmd/unix/reverse and cmd/unix/generic are supported.

The United States is experiencing a significant shift in its energy landscape. Last year, utility-scale wind and solar power combined for 47 percent of new generation capacity in the U.S. Based on this expansion, 11 states now generate more than 10 percent of their electricity from solar, wind, and geothermal power, with three of these states — Iowa, South Dakota, and Kansas — exceeding 20 percent. In 2014, California became the first state in the nation to garner 5 percent of its electricity from utility-scale solar. When including hydropower, four states —Idaho, Washington, Oregon, and South Dakota — now exceed 70 percent renewables generation.

October 17 marks the 40th anniversary of the start of the 1973 Arab Oil Embargo — an event that arguably launched the United States' ongoing pursuit of a national energy policy.

Cape Town - Health Minister Zweli Mkhize on Saturday descended on the Western Cape as pressure mounted on the national government to intervene in the coastal province that has recorded more than half of the country’s Covid-19 infections. This after health portfolio committee chairperson Sibongiseni Dhlomo had called for urgent intervention in the spread of the virus while the ANC wanted a return to level 5 of lockdown. Speaking at a press briefing in Cape Town on Saturday, Mkhize said the spread of the virus in the Western Cape was not different from other provinces. He said that the national government would provide more support, such as further training of health-care workers and...

Good morning! Here is today’s summary from Nigerian Newspapers: 1. Vice President, Yemi Osinbajo, on Saturday, gave an insight into his whereabouts in the past few weeks. Osinbajo, who spoke through his spokesperson, Akande Laolu, disclosed that he has been attending to official matters on how to improve Nigeria’s economy. Laolu urged Nigerians to ignore […]

Nigerian newspapers: 10 things you need to know this Sunday morning

The Department for Business, Innovation and Skills (BIS) has revealed that the right to request flexible work arrangements will not be extended to parents of 17 year olds in April as previously announced. Contrary to some reports, the existing law p...

Since Europe reached agreement on the Agency Workers Directive in October 2008, considerable flexibility has been left to individual Member States as to how they interpret and apply its framework of legal protection for agency workers by 5 December ...

More than 1600 people have now recovered from the Covid-19 in the Western Cape, the provincial government said on Saturday.

Health Minister Zweli Mkhize on Saturday descended on the Western Cape as pressure mounted on the national government to intervene in the coastal province.

150 soldiers said to be involved in the confrontation

1. Tere Bina teaser: Salman Khan romances Jacqueline Fernandez in his ‘cheapest production’. Watch  Hindustan Times
2. Tere Bina Teaser: Salman Khan And Jacqueline Fernandez Redefine Romance  NDTV
3. Salman Khan and Jacqueline Fernandez share the inspiration behind their upcoming track 'Tere Bina' in the  Times of India
4. Inside Salman Khan's Panvel farmhouse: Actor bonds with Jacqueline, Iulia Vantur, and Waluscha - entertainment  Mid-Day
5. KBC 2020 Registrations Begin, Iulia Vantur Reacts to Marriage Rumours with Salman Khan  News18
6. View Full coverage on Google News

KASUR: One Covid-19 patient escaped from the DPS quarantine centre on Saturday. Seven days ago, Abubakkar, a resident of Gulburg Colony, was found Covid-19 positive. He was sent to the DPS quarantine...

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Rawalpindi : Once more, a 13-year-old rape victim has been left with no other options but to move mountains in pursuit of seeking justice against the culprits who had ruined her life and are now taking every advantage with illegal support of some police officials in twisting facts and realities in...

Backlight screen and faster performance are a couple of perks of this new e-reader.

A Maryland politician who said he would sue a newspaper if it printed his name, Kirby Delauter, has apologized after his threat garnered national attention, the targeted newspaper reported on Thursday.

Apex Legends might finally be getting a mobile version, at least that's what EA's latest financial briefing seems to suggest

Master signal integrity and ensure data quality

For our feature interview, Mike catches up with Ben Rayner (22:40), the long-time music writer and critic at the Toronto Star. He’s easily one of the best music writers in Canada and it’s obvious that his love of music, and writing about it, is as strong as ever. As local newspapers disappear and even the big newspapers like the Globe & Mail and National Post diminish their arts coverage and move music journalists into other beats, Ben is among the last of a dying breed. Mike and Ben chat about changing nature of music journalism in Canada and the decline of music coverage in newspapers and then get into what impact this could have on artists and the country’s music industry.

But before we chat with Ben, we also meet up with Charlie Wall-Andrews (2:48), the executive director of the SOCAN Foundation. Charlie fills us in all the various grants and programs that the SOCAN Foundation has available to artists. Then she and Mike discuss the concept of corporate social responsibility, which is an area of particular expertise for Charlie, and how it applies to the music industry. www.socanfoundation.ca.

The front pages are led by Boris Johnson’s anticipated “road map” to a new normality.

David Warner has opened up about how his wife Candice changed his approach to cricket, spurring him on to become more disciplined in a bid to develop into one of the world’s best batsmen.

New Zealand Rugby has engaged in talks with a multi-billion-dollar American investment firm about taking a stake in the struggling sport.

As the world braces for recession, a look at the four letters that could indicate the way the economy recovers.