cali Paid Maternity Leave for Teachers? California's Governor Says No Once Again By feedproxy.google.com Published On :: Mon, 14 Oct 2019 00:00:00 +0000 The bill would have given public school teachers at least six weeks of paid maternity leave. Full Article California
cali Educational Opportunities and Performance in California By feedproxy.google.com Published On :: Tue, 21 Jan 2020 00:00:00 +0000 This Quality Counts 2020 Highlights Report captures all the data you need to assess your state's performance on key educational outcomes. Full Article California
cali California schools will look very different when they reopen By feedproxy.google.com Published On :: Wed, 15 Apr 2020 00:00:00 +0000 Full Article California
cali Betsy DeVos OKs ESSA Plans for California, Utah By feedproxy.google.com Published On :: Thu, 12 Jul 2018 00:00:00 +0000 U.S. Secretary of Education Betsy DeVos green-lighted California and Utah's plans to implement the Every Student Succeed Act. That means just one state is still waiting: Florida. Full Article Utah
cali 2003 California-USC thriller available to all fans Saturday at 1:30 PT/ 2:30 MT on Pac-12 Now By sports.yahoo.com Published On :: Thu, 07 May 2020 19:06:04 GMT Download the Pac-12 Now app to watch an epic, triple-overtime 2003 battle between USC and California this Saturday at 1:30 p.m. PT/ 2:30 p.m. MT. The game will be available to all fans. Full Article video Sports
cali Die Localisation der Hautkrankheiten : histologisch und klinisch / bearbeitet von Oscar Simon. By feedproxy.google.com Published On :: Berlin : A. Hirschwald, 1873. Full Article
cali Essai sur l’alcalinitè du sang dans l’état de santé et dans quelques maladies / par J. Canard. By feedproxy.google.com Published On :: Paris : A. Parent, 1878. Full Article
cali Earthquake Scuttles Classes in Alaska, As California Students Return to School By feedproxy.google.com Published On :: Tue, 11 Dec 2018 00:00:00 +0000 While thousands of students in wildfire-ravaged Northern California resumed classes last week, thousands of others in Alaska stayed home after a 7.0 magnitude earthquake struck Nov. 30. Full Article Alaska
cali An evaluation of the California civil addict program / by William H. McGlothlin, M. Douglas Anglin, Bruce D. Wilson. By search.wellcomelibrary.org Published On :: Rockville, Maryland : National Institute on Drug Abuse, 1977. Full Article
cali Perturbation Bounds for Procrustes, Classical Scaling, and Trilateration, with Applications to Manifold Learning By Published On :: 2020 One of the common tasks in unsupervised learning is dimensionality reduction, where the goal is to find meaningful low-dimensional structures hidden in high-dimensional data. Sometimes referred to as manifold learning, this problem is closely related to the problem of localization, which aims at embedding a weighted graph into a low-dimensional Euclidean space. Several methods have been proposed for localization, and also manifold learning. Nonetheless, the robustness property of most of them is little understood. In this paper, we obtain perturbation bounds for classical scaling and trilateration, which are then applied to derive performance bounds for Isomap, Landmark Isomap, and Maximum Variance Unfolding. A new perturbation bound for procrustes analysis plays a key role. Full Article
cali Subjective Bayesian testing using calibrated prior probabilities By projecteuclid.org Published On :: Mon, 26 Aug 2019 04:00 EDT Dan J. Spitzner. Source: Brazilian Journal of Probability and Statistics, Volume 33, Number 4, 861--893.Abstract: This article proposes a calibration scheme for Bayesian testing that coordinates analytically-derived statistical performance considerations with expert opinion. In other words, the scheme is effective and meaningful for incorporating objective elements into subjective Bayesian inference. It explores a novel role for default priors as anchors for calibration rather than substitutes for prior knowledge. Ideas are developed for use with multiplicity adjustments in multiple-model contexts, and to address the issue of prior sensitivity of Bayes factors. Along the way, the performance properties of an existing multiplicity adjustment related to the Poisson distribution are clarified theoretically. Connections of the overall calibration scheme to the Schwarz criterion are also explored. The proposed framework is examined and illustrated on a number of existing data sets related to problems in clinical trials, forensic pattern matching, and log-linear models methodology. Full Article
cali A brief review of optimal scaling of the main MCMC approaches and optimal scaling of additive TMCMC under non-regular cases By projecteuclid.org Published On :: Mon, 04 Mar 2019 04:00 EST Kushal K. Dey, Sourabh Bhattacharya. Source: Brazilian Journal of Probability and Statistics, Volume 33, Number 2, 222--266.Abstract: Transformation based Markov Chain Monte Carlo (TMCMC) was proposed by Dutta and Bhattacharya ( Statistical Methodology 16 (2014) 100–116) as an efficient alternative to the Metropolis–Hastings algorithm, especially in high dimensions. The main advantage of this algorithm is that it simultaneously updates all components of a high dimensional parameter using appropriate move types defined by deterministic transformation of a single random variable. This results in reduction in time complexity at each step of the chain and enhances the acceptance rate. In this paper, we first provide a brief review of the optimal scaling theory for various existing MCMC approaches, comparing and contrasting them with the corresponding TMCMC approaches.The optimal scaling of the simplest form of TMCMC, namely additive TMCMC , has been studied extensively for the Gaussian proposal density in Dey and Bhattacharya (2017a). Here, we discuss diffusion-based optimal scaling behavior of additive TMCMC for non-Gaussian proposal densities—in particular, uniform, Student’s $t$ and Cauchy proposals. Although we could not formally prove our diffusion result for the Cauchy proposal, simulation based results lead us to conjecture that at least the recipe for obtaining general optimal scaling and optimal acceptance rate holds for the Cauchy case as well. We also consider diffusion based optimal scaling of TMCMC when the target density is discontinuous. Such non-regular situations have been studied in the case of Random Walk Metropolis Hastings (RWMH) algorithm by Neal and Roberts ( Methodology and Computing in Applied Probability 13 (2011) 583–601) using expected squared jumping distance (ESJD), but the diffusion theory based scaling has not been considered. We compare our diffusion based optimally scaled TMCMC approach with the ESJD based optimally scaled RWM with simulation studies involving several target distributions and proposal distributions including the challenging Cauchy proposal case, showing that additive TMCMC outperforms RWMH in almost all cases considered. Full Article
cali Gun Rights: California Gun Owners & Ammo Dealers Fire Back Against... By www.prweb.com Published On :: Ammunition Depot comments on Judge Roger T. Benitez ruling that Californians may again purchase ammo without a background check and order ammo online.(PRWeb April 24, 2020)Read the full story at https://www.prweb.com/releases/gun_rights_california_gun_owners_ammo_dealers_fire_back_against_proposition_63/prweb17075447.htm Full Article
cali Modeling wildfire ignition origins in southern California using linear network point processes By projecteuclid.org Published On :: Wed, 15 Apr 2020 22:05 EDT Medha Uppala, Mark S. Handcock. Source: The Annals of Applied Statistics, Volume 14, Number 1, 339--356.Abstract: This paper focuses on spatial and temporal modeling of point processes on linear networks. Point processes on linear networks can simply be defined as point events occurring on or near line segment network structures embedded in a certain space. A separable modeling framework is introduced that posits separate formation and dissolution models of point processes on linear networks over time. While the model was inspired by spider web building activity in brick mortar lines, the focus is on modeling wildfire ignition origins near road networks over a span of 14 years. As most wildfires in California have human-related origins, modeling the origin locations with respect to the road network provides insight into how human, vehicular and structural densities affect ignition occurrence. Model results show that roads that traverse different types of regions such as residential, interface and wildland regions have higher ignition intensities compared to roads that only exist in each of the mentioned region types. Full Article
cali Microsimulation model calibration using incremental mixture approximate Bayesian computation By projecteuclid.org Published On :: Wed, 27 Nov 2019 22:01 EST Carolyn M. Rutter, Jonathan Ozik, Maria DeYoreo, Nicholson Collier. Source: The Annals of Applied Statistics, Volume 13, Number 4, 2189--2212.Abstract: Microsimulation models (MSMs) are used to inform policy by predicting population-level outcomes under different scenarios. MSMs simulate individual-level event histories that mark the disease process (such as the development of cancer) and the effect of policy actions (such as screening) on these events. MSMs often have many unknown parameters; calibration is the process of searching the parameter space to select parameters that result in accurate MSM prediction of a wide range of targets. We develop Incremental Mixture Approximate Bayesian Computation (IMABC) for MSM calibration which results in a simulated sample from the posterior distribution of model parameters given calibration targets. IMABC begins with a rejection-based ABC step, drawing a sample of points from the prior distribution of model parameters and accepting points that result in simulated targets that are near observed targets. Next, the sample is iteratively updated by drawing additional points from a mixture of multivariate normal distributions and accepting points that result in accurate predictions. Posterior estimates are obtained by weighting the final set of accepted points to account for the adaptive sampling scheme. We demonstrate IMABC by calibrating CRC-SPIN 2.0, an updated version of a MSM for colorectal cancer (CRC) that has been used to inform national CRC screening guidelines. Full Article
cali Spatio-temporal short-term wind forecast: A calibrated regime-switching method By projecteuclid.org Published On :: Wed, 16 Oct 2019 22:03 EDT Ahmed Aziz Ezzat, Mikyoung Jun, Yu Ding. Source: The Annals of Applied Statistics, Volume 13, Number 3, 1484--1510.Abstract: Accurate short-term forecasts are indispensable for the integration of wind energy in power grids. On a wind farm, local wind conditions exhibit sizeable variations at a fine temporal resolution. Existing statistical models may capture the in-sample variations in wind behavior, but are often shortsighted to those occurring in the near future, that is, in the forecast horizon. The calibrated regime-switching method proposed in this paper introduces an action of regime dependent calibration on the predictand (here the wind speed variable), which helps correct the bias resulting from out-of-sample variations in wind behavior. This is achieved by modeling the calibration as a function of two elements: the wind regime at the time of the forecast (and the calibration is therefore regime dependent), and the runlength, which is the time elapsed since the last observed regime change. In addition to regime-switching dynamics, the proposed model also accounts for other features of wind fields: spatio-temporal dependencies, transport effect of wind and nonstationarity. Using one year of turbine-specific wind data, we show that the calibrated regime-switching method can offer a wide margin of improvement over existing forecasting methods in terms of both wind speed and power. Full Article
cali Scaling limits for super-replication with transient price impact By projecteuclid.org Published On :: Mon, 27 Apr 2020 04:02 EDT Peter Bank, Yan Dolinsky. Source: Bernoulli, Volume 26, Number 3, 2176--2201.Abstract: We prove a scaling limit theorem for the super-replication cost of options in a Cox–Ross–Rubinstein binomial model with transient price impact. The correct scaling turns out to keep the market depth parameter constant while resilience over fixed periods of time grows in inverse proportion with the duration between trading times. For vanilla options, the scaling limit is found to coincide with the one obtained by PDE-methods in ( Math. Finance 22 (2012) 250–276) for models with purely temporary price impact. These models are a special case of our framework and so our probabilistic scaling limit argument allows one to expand the scope of the scaling limit result to path-dependent options. Full Article
cali Operator-scaling Gaussian random fields via aggregation By projecteuclid.org Published On :: Tue, 26 Nov 2019 04:00 EST Yi Shen, Yizao Wang. Source: Bernoulli, Volume 26, Number 1, 500--530.Abstract: We propose an aggregated random-field model, and investigate the scaling limits of the aggregated partial-sum random fields. In this model, each copy in the aggregation is a $pm 1$-valued random field built from two correlated one-dimensional random walks, the law of each determined by a random persistence parameter. A flexible joint distribution of the two parameters is introduced, and given the parameters the two correlated random walks are conditionally independent. For the aggregated random field, when the persistence parameters are independent, the scaling limit is a fractional Brownian sheet. When the persistence parameters are tail-dependent, characterized in the framework of multivariate regular variation, the scaling limit is more delicate, and in particular depends on the growth rates of the underlying rectangular region along two directions: at different rates different operator-scaling Gaussian random fields appear as the region area tends to infinity. In particular, at the so-called critical speed, a large family of Gaussian random fields with long-range dependence arise in the limit. We also identify four different regimes at non-critical speed where fractional Brownian sheets arise in the limit. Full Article
cali Calif. Ed-Tech Consortium Seeks Media Repository Solutions; Saint Paul District Needs Background Check Services By marketbrief.edweek.org Published On :: Fri, 08 May 2020 13:52:21 +0000 Saint Paul schools are in the market for a vendor to provide background checks, while the Education Technology Joint Powers Authority is seeking media repositories. A Texas district wants quotes on technology for new campuses. The post Calif. Ed-Tech Consortium Seeks Media Repository Solutions; Saint Paul District Needs Background Check Services appeared first on Market Brief. Full Article Purchasing Alert Background Checks Media Repository Procurement / Purchasing / RFPs Software / Hardware
cali Calibration Procedures for Approximate Bayesian Credible Sets By projecteuclid.org Published On :: Thu, 19 Dec 2019 22:10 EST Jeong Eun Lee, Geoff K. Nicholls, Robin J. Ryder. Source: Bayesian Analysis, Volume 14, Number 4, 1245--1269.Abstract: We develop and apply two calibration procedures for checking the coverage of approximate Bayesian credible sets, including intervals estimated using Monte Carlo methods. The user has an ideal prior and likelihood, but generates a credible set for an approximate posterior based on some approximate prior and likelihood. We estimate the realised posterior coverage achieved by the approximate credible set. This is the coverage of the unknown “true” parameter if the data are a realisation of the user’s ideal observation model conditioned on the parameter, and the parameter is a draw from the user’s ideal prior. In one approach we estimate the posterior coverage at the data by making a semi-parametric logistic regression of binary coverage outcomes on simulated data against summary statistics evaluated on simulated data. In another we use Importance Sampling from the approximate posterior, windowing simulated data to fall close to the observed data. We illustrate our methods on four examples. Full Article
cali Jointly Robust Prior for Gaussian Stochastic Process in Emulation, Calibration and Variable Selection By projecteuclid.org Published On :: Tue, 11 Jun 2019 04:00 EDT Mengyang Gu. Source: Bayesian Analysis, Volume 14, Number 3, 877--905.Abstract: Gaussian stochastic process (GaSP) has been widely used in two fundamental problems in uncertainty quantification, namely the emulation and calibration of mathematical models. Some objective priors, such as the reference prior, are studied in the context of emulating (approximating) computationally expensive mathematical models. In this work, we introduce a new class of priors, called the jointly robust prior, for both the emulation and calibration. This prior is designed to maintain various advantages from the reference prior. In emulation, the jointly robust prior has an appropriate tail decay rate as the reference prior, and is computationally simpler than the reference prior in parameter estimation. Moreover, the marginal posterior mode estimation with the jointly robust prior can separate the influential and inert inputs in mathematical models, while the reference prior does not have this property. We establish the posterior propriety for a large class of priors in calibration, including the reference prior and jointly robust prior in general scenarios, but the jointly robust prior is preferred because the calibrated mathematical model typically predicts the reality well. The jointly robust prior is used as the default prior in two new R packages, called “RobustGaSP” and “RobustCalibration”, available on CRAN for emulation and calibration, respectively. Full Article
cali ROS Regression: Integrating Regularization with Optimal Scaling Regression By projecteuclid.org Published On :: Fri, 11 Oct 2019 04:03 EDT Jacqueline J. Meulman, Anita J. van der Kooij, Kevin L. W. Duisters. Source: Statistical Science, Volume 34, Number 3, 361--390.Abstract: We present a methodology for multiple regression analysis that deals with categorical variables (possibly mixed with continuous ones), in combination with regularization, variable selection and high-dimensional data ($Pgg N$). Regularization and optimal scaling (OS) are two important extensions of ordinary least squares regression (OLS) that will be combined in this paper. There are two data analytic situations for which optimal scaling was developed. One is the analysis of categorical data, and the other the need for transformations because of nonlinear relationships between predictors and outcome. Optimal scaling of categorical data finds quantifications for the categories, both for the predictors and for the outcome variables, that are optimal for the regression model in the sense that they maximize the multiple correlation. When nonlinear relationships exist, nonlinear transformation of predictors and outcome maximize the multiple correlation in the same way. We will consider a variety of transformation types; typically we use step functions for categorical variables, and smooth (spline) functions for continuous variables. Both types of functions can be restricted to be monotonic, preserving the ordinal information in the data. In combination with optimal scaling, three popular regularization methods will be considered: Ridge regression, the Lasso and the Elastic Net. The resulting method will be called ROS Regression (Regularized Optimal Scaling Regression). The OS algorithm provides straightforward and efficient estimation of the regularized regression coefficients, automatically gives the Group Lasso and Blockwise Sparse Regression, and extends them by the possibility to maintain ordinal properties in the data. Extended examples are provided. Full Article
cali California Bats Thrive in Forests Recovering From Wildfires By www.smithsonianmag.com Published On :: Tue, 10 Mar 2020 20:29:27 +0000 Wildfires leave behind a patchwork of forest densities that can give bats more room to fly and hunt Full Article
cali In a First, Researchers Record Penguins Vocalizing Under Water By www.smithsonianmag.com Published On :: Mon, 23 Mar 2020 11:00:00 +0000 But the scientists still aren’t sure what the birds are saying Full Article
cali Dolphins, Surfers and Waves Sparkle in Bright Blue Bioluminescent Glow Off California Coast By www.smithsonianmag.com Published On :: Tue, 28 Apr 2020 16:31:43 +0000 A rare bloom of microscopic organisms capable of making their own blue light has transformed several of the state’s beaches Full Article
cali Comment on Rock-Oper "Excalibur" wieder auf Tour by Celtic Grove Rock By rss-newsfeed.india-meets-classic.net Published On :: Thu, 28 Oct 2010 17:49:39 +0000 <span class="topsy_trackback_comment"><span class="topsy_twitter_username"><span class="topsy_trackback_content">Celtic Rock News - Rock-Oper "Excalibur" wieder auf Tour | RSS Feeds – IMC OnAir ...: Rock-Oper "Ex... http://bit.ly/bLZX6K celticgrove.com</span></span> Full Article
cali These California Wineries Are Hosting Virtual Wine Tastings By www.smithsonianmag.com Published On :: Fri, 10 Apr 2020 12:00:00 +0000 Sheltering in place doesn’t mean you have to give up the best of wine country's offerings Full Article
cali Gerard Richardson: Californian reds By www.heraldscotland.com Published On :: Sat, 21 Sep 2019 05:00:00 +0100 WELL, I don't know about you but my central heating has already been on twice in September and although I don't need much of an excuse to delve into the big heavy reds, two heating days is my current one. Full Article
cali Citing Class Sizes, Services, Teachers In California's Capital City Walk Out By feedproxy.google.com Published On :: Tue, 16 Apr 2019 00:00:00 +0000 Hundreds of teachers across the Sacramento Unified school district walked out of their classrooms and onto picket lines last week for the first time in 30 years, staging a one-day strike alleging unfair labor practices by the California district. Full Article Class+size
cali Incoming California Governor to Seek Nearly $2 Billion in Early-Childhood Funding By feedproxy.google.com Published On :: Fri, 04 Jan 2019 00:00:00 +0000 Democrat Gavin Newsom, who takes office Jan. 7, plans to expand full-day kindergarten and child-care offerings in the state, according to media reports. Full Article Earlychildhood
cali Bilingual Education Poised for a Comeback in California Schools By feedproxy.google.com Published On :: Tue, 11 Oct 2016 00:00:00 +0000 Nearly 20 years after voting to restrict bilingual education in a state with more than 1 million schoolchildren who don't speak English as their first language, California voters appear poised to reverse that ban. Full Article Bilingual+education
cali California Voters Repeal Ban on Bilingual Education By feedproxy.google.com Published On :: Tue, 08 Nov 2016 00:00:00 +0000 The ballot measure essentially repeals Proposition 227, the 1998 law that made it tougher for districts to offer bilingual education. Full Article Bilingual+education
cali Bilingual Education in California? State Voters to Decide (Video) By feedproxy.google.com Published On :: Mon, 07 Nov 2016 00:00:00 +0000 In California, nearly 1 in every 4 children don't speak English fluently. On Election Day, the state's voters will decide whether to overturn a longstanding policy of teaching these children in English-only classrooms, or whether to embrace bilingual forms of teaching. Full Article Bilingual+education
cali Bilingual Education Set to Return to California Schools By feedproxy.google.com Published On :: Tue, 15 Nov 2016 00:00:00 +0000 After nearly two decades of restrictions, educators say it will take time for schools to create new programs and hire bilingual teachers. Full Article Bilingual+education
cali Closely Spaced Pregnancies Are Associated With Increased Odds of Autism in California Sibling Births By pediatrics.aappublications.org Published On :: 2011-01-10T04:01:22-08:00 Autism has been associated with pregnancy and birth complications that may indicate a suboptimal prenatal environment. Although the interpregnancy interval (IPI) may affect the prenatal environment, the association between the IPI and risk for autism is not known. Using full-sibling pairs from a large population, the authors examined the association between autism and IPIs. Second-born children conceived after an IPI of <12 months had more than threefold increased odds of autism relative to those with IPIs of ≥36 months. (Read the full article) Full Article
cali Nonmedical Vaccine Exemptions and Pertussis in California, 2010 By pediatrics.aappublications.org Published On :: 2013-09-30T00:06:52-07:00 Previous studies have shown that nonmedical exemptions (NMEs) to immunization cluster geographically and contribute to outbreaks of vaccine-preventable diseases such as pertussis. The 2010 pertussis resurgence in California has been widely attributed to waning immunity from acellular pertussis vaccines.This study provides evidence of spatial and temporal clustering of NMEs and clustering of pertussis cases and suggests that geographic areas with high NME rates were also associated with high rates of pertussis in California in 2010. (Read the full article) Full Article
cali Personal Belief Exemptions to Vaccination in California: A Spatial Analysis By pediatrics.aappublications.org Published On :: 2015-06-01T06:07:55-07:00 An increasing number of children are unvaccinated at entry into public schools, potentially endangering children who cannot be vaccinated for medical reasons and threatening herd immunity. Voluntary exemptions from immunizations vary geographically and by parental characteristics.We find that exemption behavior is highest in peripheral areas of cities and that specific types of student populations are associated with high exemption rates. Additionally, there is spatial overlap between clusters of high personal exemption and medical exemption populations. (Read the full article) Full Article
cali Calidore String Quartet By news.psu.edu Published On :: Tue, 10 Mar 2020 11:40 -0400 Full Article
cali Cameroon: The Threat of Religious Radicalism By feedproxy.google.com Published On :: Thu, 03 Sep 2015 09:56:00 GMT Religious intolerance is a growing but seriously underestimated risk in Cameroon, both between and inside the major faiths. To halt the spread of violent extremism in the country, Cameroon needs to bring all sects into a new social compact and within the bounds of a charter for religious tolerance. Full Article
cali Cameroun : au-delà de Boko Haram, la menace insidieuse du radicalisme religieux By feedproxy.google.com Published On :: Wed, 16 Sep 2015 22:00:00 GMT L’image de havre de paix dans une région en proie aux conflits dont bénéficiait le Cameroun a volé en éclats depuis l’irruption de Boko Haram en 2013 au nord du pays. Ce mouvement, devenu l’Etat islamique en Afrique de l’Ouest en mars 2015, revendique son affiliation à Daech. Néanmoins, l’apparition brutale et sanglante de ce djihadisme africain est moins liée à l’essor de Daech en Irak et en Syrie qu’aux bouleversements du paysage religieux de l’Afrique en général et du Cameroun en particulier. Full Article
cali California Moves Toward New Test-Score Reporting By feedproxy.google.com Published On :: Fri, 05 Dec 2014 00:00:00 +0000 Federal law requires states to report student test scores in achievement levels, but leaders in the Golden State want to take a different approach. Full Article Assessment+Accountability+Achievement
cali Transferable Resistance Gene optrA in Enterococcus faecalis from Swine in Brazil [Mechanisms of Resistance] By aac.asm.org Published On :: 2020-04-06T08:49:25-07:00 OptrA is an ATP-binding cassette (ABC)-F protein that confers resistance to oxazolidinones and phenicols, and can be either plasmid or chromosomally encoded. We isolated 13 Enterococcus faecalis strains possessing linezolid MIC ≥ 4 mg/L from nursery pigs in swine herds located across Brazil. Genome sequence comparison showed that these strains possess optrA in different genetic contexts occurring in 5 different E. faecalis sequence type backgrounds. The optrA gene invariably occurred in association with an araC regulator and a gene encoding a hypothetical protein. In some contexts, this genetic island was able to excise and form a covalently closed circle within the cell which appeared to occur in high abundance, and to be transmissible by co-resident plasmids. Full Article
cali Structural recognition of spectinomycin by resistance enzyme ANT(9) from Enterococcus faecalis [Mechanisms of Resistance] By aac.asm.org Published On :: 2020-04-06T08:49:25-07:00 Spectinomycin is a ribosome-binding antibiotic that blocks the translocation step of translation. A prevalent resistance mechanism is the modification of the drug by aminoglycoside nucleotidyl transferase (ANT) enzymes of the spectinomycin-specific ANT (9) family or by the dual-specificity ANT(3") (9) family that also acts on streptomycin. We previously reported the structural mechanism of streptomycin modification by the ANT(3") (9) AadA from Salmonella enterica. ANT (9) from Enterococcus faecalis adenylates the 9-hydroxyl of spectinomycin. We here present the first structures of spectinomycin bound to an ANT enzyme. Structures were solved for ANT (9) in apo form, in complex with ATP, spectinomycin and magnesium or in complex with only spectinomycin. ANT (9) shows similar overall structure as AadA with an N-terminal nucleotidyltransferase domain and a C-terminal α-helical domain. Spectinomycin binds close to the entrance of the interdomain cleft, while ATP is buried at the bottom. Upon drug binding, the C-terminal domain rotates by 14 degrees to close the cleft, allowing contacts of both domains with the drug. Comparison with AadA shows that spectinomycin specificity is explained by a straight α5 helix and a shorter α5-α6 loop that would clash with the larger streptomycin substrate. In the active site, we observe two magnesium ions, one of them in a previously un-observed position that may activate the 9-hydroxyl for deprotonation by the catalytic base Glu-86. The observed binding mode for spectinomycin suggests that also spectinamides and aminomethyl spectinomycins, recent spectinomycin analogues with expansions in position 4 of the C ring, will be subjected to modification by ANT (9) and ANT(3") (9) enzymes. Full Article
cali Fin24.com | 3 'practicalities' to consider before investing in property in Africa – expert By www.fin24.com Published On :: Sun, 10 Nov 2019 16:45:17 +0200 Africa can be a challenging place to do business and property investment is not exempt from some, often unique, challenges, says a Nedbank expert. Full Article
cali Tamsin Calidas: Memoir lays bare life on a Hebridean croft By www.heraldscotland.com Published On :: Sat, 02 May 2020 05:00:00 +0100 WHEN Tamsin Calidas stepped aboard a ferry bound for the Hebridean island that would become her new home, it was with hope for a fresh beginning, one far from the tumultuous events and near-death experience she had faced in the city streets being left behind. Full Article
cali Reading Instruction 'Keeps Parents Up at Night': Advocates in Wis., Calif. Push for Changes By feedproxy.google.com Published On :: Wed, 19 Feb 2020 00:00:00 +0000 As schools apply more scrutiny to the methods and materials they use to teach early reading, educators and parents in some states have started to form new advocacy efforts—trying to pressure states and districts to adopt new approaches to teacher training and evaluating materials. Full Article Reading
cali California Schools Superintendent: Curriculum Cuts Will Undermine Instruction By feedproxy.google.com Published On :: Tue, 06 Oct 2009 00:00:00 +0000 California's budget reductions will result in some state curricular materials not reaching the state's schools until 2017 or later, Jack O'Connell says. Full Article Curriculum+and+instruction
cali California's Ethnic Studies Curriculum, Criticized for 'Anti-Jewish Bias,' to Be Revised By feedproxy.google.com Published On :: Wed, 14 Aug 2019 00:00:00 +0000 California's proposed curriculum guide in ethnic studies is being sent back for substantial revision after a pileup of criticism that it's anti-Semitic. Full Article Curriculum+and+instruction
cali Reading Instruction 'Keeps Parents Up at Night': Advocates in Wis., Calif. Push for Changes By feedproxy.google.com Published On :: Wed, 19 Feb 2020 00:00:00 +0000 As schools apply more scrutiny to the methods and materials they use to teach early reading, educators and parents in some states have started to form new advocacy efforts—trying to pressure states and districts to adopt new approaches to teacher training and evaluating materials. Full Article Curriculum+and+instruction