Comedian Matt Rogers wants Mariah Carey to crown him Prince of Christmas; the best TV of 2022 and what to catch up on over the holidays; why The Muppet Christmas Carol is the best retelling of Scrooge’s story; Revival 69, the improbable rock show that put Canada on the map and helped end the Beatles; and more.

On Friday night, after I appeared on Alex Wagner’s MSNBC show to talk about my essay “Lessons on media policy at the slaughter-bench of history,” the number of subscribers to this newsletter exploded and nearly doubled overnight. via Pocket

Knowing how to fish is among the greatest assets anyone can possess when survival is on the line (pun intended)

The post Important to know! Survival Fishing: How to Catch Fish When SHTF appeared first on Powdered Wig Society.

Hungary have ridden back into contention for international honours on the back of billions of euros and one man's passion for football.

Reactive oxygen species (ROS) are an unavoidable host environmental cue for intracellular pathogens such as Mycobacterium tuberculosis and Mycobacterium bovis; however, the signaling pathway in mycobacteria for sensing and responding to environmental stress remains largely unclear. Here, we characterize a novel CmtR-Zur-ESX3-Zn2+ regulatory pathway in M. bovis that aids mycobacterial survival under oxidative stress. We demonstrate that CmtR functions as a novel redox sensor and that its expression can be significantly induced under H2O2 stress. CmtR can physically interact with the negative regulator Zur and de-represses the expression of the esx-3 operon, which leads to Zn2+ accumulation and promotion of reactive oxygen species detoxication in mycobacterial cells. Zn2+ can also act as an effector molecule of the CmtR regulator, using which the latter can de-repress its own expression for further inducing bacterial antioxidant adaptation. Consistently, CmtR can induce the expression of EsxH, a component of esx-3 operon involved in Zn2+ transportation that has been reported earlier, and inhibit phagosome maturation in macrophages. Lastly, CmtR significantly contributes to bacterial survival in macrophages and in the lungs of infected mice. Our findings reveal the existence of an antioxidant regulatory pathway in mycobacteria and provide novel information on stress-triggered gene regulation and its association with host–pathogen interaction.

Visual Abstract

RAS genes are the most commonly mutated in human cancers and play critical roles in tumor initiation, progression, and drug resistance. Identification of targets that block RAS signaling is pivotal to develop therapies for RAS-related cancer. As RAS translocation to the plasma membrane (PM) is essential for its effective signal transduction, we devised a high-content screening assay to search for genes regulating KRAS membrane association. We found that the tyrosine phosphatase PTPN2 regulates the plasma membrane localization of KRAS. Knockdown of PTPN2 reduced the proliferation and promoted apoptosis in KRAS-dependent cancer cells, but not in KRAS-independent cells. Mechanistically, PTPN2 negatively regulates tyrosine phosphorylation of KRAS, which, in turn, affects the activation KRAS and its downstream signaling. Consistently, analysis of the TCGA database demonstrates that high expression of PTPN2 is significantly associated with poor prognosis of patients with KRAS-mutant pancreatic adenocarcinoma. These results indicate that PTPN2 is a key regulator of KRAS and may serve as a new target for therapy of KRAS-driven cancer.

Stephanie E. Hood
Dec 1, 2020; 61:1720-1732
Research Articles

Lipins are eukaryotic proteins with functions in lipid synthesis and the homeostatic control of energy balance. They execute these functions by acting as phosphatidate phosphatase enzymes in the cytoplasm and by changing gene expression after translocation into the cell nucleus, in particular under fasting conditions. Here, we asked whether nuclear translocation and the enzymatic activity of Drosophila Lipin serve essential functions and how gene expression changes, under both fed and fasting conditions, when nuclear translocation is impaired. To address these questions, we created a Lipin null mutant, a mutant expressing Lipin lacking a nuclear localization signal (Lipin^NLS), and a mutant expressing enzymatically dead Lipin. Our data support the conclusion that the enzymatic but not nuclear gene regulatory activity of Lipin is essential for survival. Notably, adult Lipin^NLS flies were not only viable but also exhibited improved life expectancy. In contrast, they were highly susceptible to starvation. Both the improved life expectancy in the fed state and the decreased survival in the fasting state correlated with changes in metabolic gene expression. Moreover, increased life expectancy of fed flies was associated with a decreased metabolic rate. Interestingly, in addition to metabolic genes, genes involved in feeding behavior and the immune response were misregulated in Lipin^NLS flies. Altogether, our data suggest that the nuclear activity of Lipin influences the genomic response to nutrient availability with effects on life expectancy and starvation resistance. Thus, nutritional or therapeutic approaches that aim at lowering nuclear translocation of lipins in humans may be worth exploring.

"Georgia is not heard or seen in the world, and many don't even know the location of the country," says TED Fellow and photographer Daro Sulakauri. Through her striking photographs of life in the shadows of Russian occupation, she uses her camera to create a living archive of her home country and defend against the erasure of its borders, culture and history.

The world’s slowest mammal is at risk of extinction by the end of the century due to their low metabolic rate and climate change

“Looking around that pub at the various people enjoying their tea, I started really thinking about what he’d said,” Hannah remembers. “Praying for revival…In a country that many have come to learn is spiritually dark, have we given up on praying for the people? Has the belief in revival died, or are we still trusting that God can move in a mighty way?”

Mukesh Khanna will be reprising the role of Indian superhero Shaktimaan in the upcoming show

Read the in depth Review of Sony Ericsson Vivaz Pro: Loses 8MP camera, gets a keyboard - at Rs. 24,585 Mobile Phones. Know detailed info about Sony Ericsson Vivaz Pro: Loses 8MP camera, gets a keyboard - at Rs. 24,585 configuration, design and performance quality along with pros & cons, Digit rating, verdict based on user opinions/feedback.

Hi! I've made some 1-point waveform "markers" that I want to overlay in my plots to aid visualization (with the added advantage, w.r.t. normal Viva markers, that they update location automatically upon refreshing simulation data).

For example, the plot below shows an spectrum along with two of these markers, which I create with the function "singlePointWave", and the Assembler output definitions also as shown below.

The problem is: as currently created and defined, Assembler is unable to plot these elements. I can send their expressions to the calculator and plotting works from there, BUT ONLY after first enabling the "Allow Any Units" in the target Viva subwindow.

Thus, I suspect Assembler is failing to plot my markers because they "lack" other information like axes units and so on. How could I add whatever is missing, so that these markers can plot automatically from Assembler?

Thanks in advance for any help!

Jorge.

P.S. I also don't know why, but nothing works without those "ymax()" in the output definitions--I suspect they are somehow converting the arguments to the right data type expected by singlePointWave(). Ideas how to fix that are also welcome! ^^

procedure( singlePointWave(xVal yVal)
    let( (xVect yVect wave)
        xVect = drCreateVec('double list(xVal));
        yVect = drCreateVec('double list(yVal));
        wave = drCreateWaveform(xVect yVect);
    );
);

Feeding bees edible bits of hydrogel increases their odds of surviving pesticide exposure by 30 per cent

Tribalism can be toxic, yet we need more of it if we are to meet today’s global challenges, argues one anthropologist. His research reveals how to create a “teratribe”

Title: Survival Rates Better for Certain Heart Transplant Patients
Category: Health News
Created: 8/24/2010 6:10:00 PM
Last Editorial Review: 8/25/2010 12:00:00 AM

Title: Survival More Likely With Exercise-Related Cardiac Arrest: Study
Category: Health News
Created: 8/30/2012 6:05:00 PM
Last Editorial Review: 8/31/2012 12:00:00 AM

Title: Blood Pressure Drugs Linked to Longer Ovarian Cancer Survival
Category: Health News
Created: 8/24/2015 12:00:00 AM
Last Editorial Review: 8/25/2015 12:00:00 AM

Title: Oxygen Therapy Doesn't Boost Heart Attack Survival
Category: Health News
Created: 8/30/2017 12:00:00 AM
Last Editorial Review: 8/31/2017 12:00:00 AM

Title: Scientists Challenge Key Survival Stat Cited by U.S. Officials in Plasma Approval
Category: Health News
Created: 8/25/2020 12:00:00 AM
Last Editorial Review: 8/25/2020 12:00:00 AM

Title: Blood Pressure Meds Could Improve Survival in COVID-19 Patients
Category: Health News
Created: 8/24/2020 12:00:00 AM
Last Editorial Review: 8/25/2020 12:00:00 AM

Intrapatient intermetastatic heterogeneity (IIH) has been demonstrated in metastatic castration-resistant prostate cancer (mCRPC) patients and is of the utmost importance for radiopharmaceutical therapy (RPT) eligibility. This study was designed to determine the prevalence of IIH and RPT eligibility in mCRPC patients through a triple-tracer PET imaging strategy. Methods: This was a multisite prospective observational study in which mCRPC patients underwent both ¹⁸F-FDG and ⁶⁸Ga-prostate-specific membrane antigen (PSMA)–617 PET/CT scans. A third scan with ⁶⁸Ga-DOTATATE, a potential biomarker of neuroendocrine differentiation, was performed if an ¹⁸F-FDG–positive/⁶⁸Ga-PSMA–negative lesion was found. Per-tracer lesion positivity was defined as having an uptake at least 50% above that of the liver. IIH prevalence was defined as the percentage of participants having at least 2 lesions with discordant features on multitracer PET. Results: IIH was observed in 81 patients (82.7%), and at least 1 ¹⁸F-FDG–positive/⁶⁸Ga-PSMA–negative lesion was found in 45 patients (45.9%). Of the 37 participants who also underwent ⁶⁸Ga-DOTATATE PET/CT, 6 (16.2%) had at least 1 ⁶⁸Ga-DOTATATE–positive lesion. In total, 12 different combinations of lesion imaging phenotypes were observed. On the basis of our prespecified criteria, 52 (53.1%) participants were determined to be eligible for PSMA RPT, but none for DOTATATE RPT. Patients with IIH had a significantly shorter median overall survival than patients without IIH (9.5 mo vs. not reached; log-rank P = 0.03; hazard ratio, 2.7; 95% CI, 1.1–6.8). Conclusion: Most mCRPC patients showed IIH, which was associated with shorter overall survival. On the basis of a triple-tracer PET approach, multiple phenotypic combinations were found. Correlation of these imaging phenotypes with genomics and treatment response will be relevant for precision medicine.

The woke and overcommitted military of the last couple decades has had a hard time recruiting. We are ripe for a change.

Facing an unprecedented crisis, IT World Canada, a beacon of technology journalism, is on the brink of closure. This poses a critical question: Without IT World Canada, who will narrate Canada’s tech story? After decades of serving the Canadian tech industry, IT World Canada needs help to survive. As of midnight tonight, the company is […]

Last week the organization tasked with running the the biggest chunk of U.S. CHIPS Act’s US $13 billion R&D program made some significant strides: The National Semiconductor Technology Center (NSTC) released a strategic plan and selected the sites of two of three planned facilities and released a new strategic plan. The locations of the two sites—a “design and collaboration” center in Sunnyvale, Calif., and a lab devoted to advancing the leading edge of chipmaking, in Albany, N.Y.—build on an existing ecosystem at each location, experts say. The location of the third planned center—a chip prototyping and packaging site that could be especially critical for speeding semiconductor startups—is still a matter of speculation.

“The NSTC represents a once-in-a-generation opportunity for the U.S. to accelerate the pace of innovation in semiconductor technology,” Deirdre Hanford, CEO of Natcast, the nonprofit that runs the NSTC centers, said in a statement. According to the strategic plan, which covers 2025 to 2027, the NSTC is meant to accomplish three goals: extend U.S. technology leadership, reduce the time and cost to prototype, and build and sustain a semiconductor workforce development ecosystem. The three centers are meant to do a mix of all three.

New York gets extreme ultraviolet lithography

NSTC plans to direct $825 million into the Albany project. The site will be dedicated to extreme ultraviolet lithography, a technology that’s essential to making the most advanced logic chips. The Albany Nanotech Complex, which has already seen more than $25 billion in investments from the state and industry partners over two decades, will form the heart of the future NSTC center. It already has an EUV lithography machine on site and has begun an expansion to install a next-generation version, called high-NA EUV, which promises to produce even finer chip features. Working with a tool recently installed in Europe, IBM, a long-time tenant of the Albany research facility, reported record yields of copper interconnects built every 21 nanometers, a pitch several nanometers tighter than possible with ordinary EUV.

“It’s fulfilling to see that this ecosystem can be taken to the national and global level through CHIPS Act funding,” said Mukesh Khare, general manager of IBM’s semiconductors division, speaking from the future site of the NSTC EUV center. “It’s the right time, and we have all the ingredients.”

While only a few companies are capable of manufacturing cutting edge logic using EUV, the impact of the NSTC center will be much broader, Khare argues. It will extend down as far as early-stage startups with ideas or materials for improving the chipmaking process “An EUV R&D center doesn’t mean just one machine,” says Khare. “It needs so many machines around it… It’s a very large ecosystem.”

Silicon Valley lands the design center

The design center is tasked with conducting advanced research in chip design, electronic design automation (EDA), chip and system architectures, and hardware security. It will also host the NSTC’s design enablement gateway—a program that provides NSTC members with a secure, cloud-based access to design tools, reference processes and designs, and shared data sets, with the goal of reducing the time and cost of design. Additionally, it will house workforce development, member convening, and administration functions.

Situating the design center in Silicon Valley, with its concentration of research universities, venture capital, and workforce, seems like the obvious choice to many experts. “I can’t think of a better place,” says Patrick Soheili, co-founder of interconnect technology startup Eliyan, which is based in Santa Clara, Calif.

Abhijeet Chakraborty, vice president of engineering in the technology and product group at Silicon Valley-based Synopsys, a leading maker of EDA software, sees Silicon Valley’s expansive tech ecosystem as one of its main advantages in landing the NSTC’s design center. The region concentrates companies and researchers involved in the whole spectrum of the industry from semiconductor process technology to cloud software.

Access to such a broad range of industries is increasingly important for chip design startups, he says. “To design a chip or component these days you need to go from concept to design to validation in an environment that takes care of the entire stack,” he says. It’s prohibitively expensive for a startup to do that alone, so one of Chakraborty’s hopes for the design center is that it will help startups access the design kits and other data needed to operate in this new environment.

Packaging and prototyping still to come

A third promised center for prototyping and packaging is still to come. “The big question is where does the packaging and prototyping go?” says Mark Granahan, cofounder and CEO of Pennsylvania-based power semiconductor startup Ideal Semiconductor. “To me that’s a great opportunity.” He points out that because there is so little packaging technology infrastructure in the United States, any ambitious state or region should have a shot at hosting such a center. One of the original intentions of the act, after all, was to expand the number of regions of the country that are involved in the semiconductor industry.

But that hasn’t stopped some already tech-heavy regions from wanting it. “Oregon offers the strongest ecosystem for such a facility,” a spokesperson for Intel, whose technology development is done there. “The state is uniquely positioned to contribute to the success of the NSTC and help drive technological advancements in the U.S. semiconductor industry.”

As NSTC makes progress, Granahan’s concern is that bureaucracy will expand with it and slow efforts to boost the U.S. chip industry. Already the layers of control are multiplying. The Chips Office at the National Institute of Standards and Technology executes the Act. The NSTC is administered by the nonprofit Natcast, which directs the EUV center, which is in a facility run by another nonprofit, NY CREATES. “We want these things to be agile and make local decisions.”

1. Study reveals how 3D environments affect bacterial growth and survival  The Hindu
2. 3D environments influence bacterial growth & survival  The Times of India
3. Shape Matters: New Research Reveals Impact of 3D Environments on Bacterial Growth  Research Matters
4. Study reveals role of 3D environments in influencing bacterial survival and growth  Bangalore Mirror

Featuring one of our new sweet and tangy margaritas, the Grilled Pineapple Margarita is a mix of grilled pineapple, tequila and Falernum syrup. Learn how to make your own!

Viva� and Monica Potter Unleash Clean Event Video

Dr Aluvihare

Once millions used it to find out about their family histories - this week it was almost delisted. What went wrong?

Extended lymph node removal during surgery for localized muscle-invasive medlinkbladder cancer/medlink does not improve patient survival(!--ref1--).

A new treatment that activates the immune system to fight cancer has boosted the survival rate for patients with advanced Hodgkin lymphoma to an impressive

Using a gene signature technique to customize chemotherapy for patients with early triple negative breast cancer may be a promising strategy to increase disease-free survival.

In children who have received a donor medlinkstem cell transplant/medlink, having a varied range of microorganisms in their gut before the transplant

For the first time, scientists have tracked the fate of medlinkstem cells/medlink decades after a medlinktransplant/medlink, shedding light on

Dual treatment has improved the survival rates for men with metastatic prostate cancer by an average of six months. Dual treatment includes both standard

Among young adults aged 18-39 newly diagnosed with cancer, the expanded Medicaid coverage under the Affordable Care Act (ACA) led to better survival outcomes,

1. Study reveals how 3D environments affect bacterial growth and survival  The Hindu
2. 3D environments influence bacterial growth & survival  The Times of India
3. Shape Matters: New Research Reveals Impact of 3D Environments on Bacterial Growth  Research Matters
4. Study reveals role of 3D environments in influencing bacterial survival and growth  Bangalore Mirror

Abingdon, Oxon ; New York, NY : Routledge, 2016.

Durham : Duke University Press, [2023]

Kreutzmann, Maria Bach, author

Revenue-earning freight traffic for April–Nov period was saw an 8.3 per cent higher y-o-y at 976.8 million tonnes

Assam’s Nati dance tradition is set on the path to regaining respect.

Survivorman host Les Stroud uses the power of Twitter to answer the internet's burning questions about outdoor survival. How do survivalists subsist on one bug a day? How does one keep from inhaling too much smoke from a fire? How does Les prepare for a trip? How does he keep his camera batteries charged during excursions? Host and producer of Survivorman, Les Stroud is credited with creating the genre known as Survival TV. He is an award winning producer-director of several series and an author and musician and he launches Surviving Disasters with Les Stroud (March 2021) on all PBS stations along with Les Stroud’s Wild Harvest and his new children's book: Wild Outside with Annick Press.