In a region battling worsening climate challenges, this groundbreaking, yet easily replicable climate forecasting model will transform local decision-making processes.

The post IWMI is advancing use of high-res climate modeling for Middle East and North Africa’s small basins first appeared on International Water Management Institute (IWMI).

IEEE / Institute of Electrical and Electronics Engineers Incorporated

IEEE / Institute of Electrical and Electronics Engineers Incorporated

New York, NY : Routledge, 2016.

New York : Routledge, 2020

New York, NY : Routledge/Taylor & Francis Group, 2016

Protease-activated receptor 2 (PAR-2) is activated by secreted proteases from immune cells or fungi. PAR-2 is normally expressed basolaterally in differentiated nasal ciliated cells. We hypothesized that epithelial remodeling during diseases characterized by cilial loss and squamous metaplasia may alter PAR-2 polarization. Here, using a fluorescent arrestin assay, we confirmed that the common fungal airway pathogen Aspergillus fumigatus activates heterologously-expressed PAR-2. Endogenous PAR-2 activation in submerged airway RPMI 2650 or NCI–H520 squamous cells increased intracellular calcium levels and granulocyte macrophage–colony-stimulating factor, tumor necrosis factor α, and interleukin (IL)-6 secretion. RPMI 2650 cells cultured at an air–liquid interface (ALI) responded to apically or basolaterally applied PAR-2 agonists. However, well-differentiated primary nasal epithelial ALIs responded only to basolateral PAR-2 stimulation, indicated by calcium elevation, increased cilia beat frequency, and increased fluid and cytokine secretion. We exposed primary cells to disease-related modifiers that alter epithelial morphology, including IL-13, cigarette smoke condensate, and retinoic acid deficiency, at concentrations and times that altered epithelial morphology without causing breakdown of the epithelial barrier to model early disease states. These altered primary cultures responded to both apical and basolateral PAR-2 stimulation. Imaging nasal polyps and control middle turbinate explants, we found that nasal polyps, but not turbinates, exhibit apical calcium responses to PAR-2 stimulation. However, isolated ciliated cells from both polyps and turbinates maintained basolateral PAR-2 polarization, suggesting that the calcium responses originated from nonciliated cells. Altered PAR-2 polarization in disease-remodeled epithelia may enhance apical responses and increase sensitivity to inhaled proteases.

Learn how researchers are exploring new hybrid devices that can exploit features of both supercapacitors and lithium-ion batteries.

Watch the video and learn basic principles of modeling panel data using SAS/ETS.

This paper provides recommendations for circumventing memory problems and reducing execution times for your mixed-modeling analyses, as well as showing the new HPMIXED procedure can be beneficial for certain situations, as with large sparse mixed models.

Oxidation states of individual metal atoms within a metalloprotein can be assigned by examining X-ray absorption edges, which shift to higher energy for progressively more positive valence numbers. Indeed, X-ray crystallography is well suited for such a measurement, owing to its ability to spatially resolve the scattering contributions of individual metal atoms that have distinct electronic environments contributing to protein function. However, as the magnitude of the shift is quite small, about +2 eV per valence state for iron, it has only been possible to measure the effect when performed with monochromated X-ray sources at synchrotron facilities with energy resolutions in the range 2–3 × 10−4 (ΔE/E). This paper tests whether X-ray free-electron laser (XFEL) pulses, which have a broader bandpass (ΔE/E = 3 × 10−3) when used without a monochromator, might also be useful for such studies. The program nanoBragg is used to simulate serial femtosecond crystallography (SFX) diffraction images with sufficient granularity to model the XFEL spectrum, the crystal mosaicity and the wavelength-dependent anomalous scattering factors contributed by two differently charged iron centers in the 110-amino-acid protein, ferredoxin. Bayesian methods are then used to deduce, from the simulated data, the most likely X-ray absorption curves for each metal atom in the protein, which agree well with the curves chosen for the simulation. The data analysis relies critically on the ability to measure the incident spectrum for each pulse, and also on the nanoBragg simulator to predict the size, shape and intensity profile of Bragg spots based on an underlying physical model that includes the absorption curves, which are then modified to produce the best agreement with the simulated data. This inference methodology potentially enables the use of SFX diffraction for the study of metalloenzyme mechanisms and, in general, offers a more detailed approach to Bragg spot data reduction.

Ba excess in LaBaGaO4 triggers ionic conductivity together with structural disorder. A direct correlation is found between the density functional theory model energy and the pair distribution function fit residual.

Aparna Sherlekar, Gayatri Mundhe, Prachi Richa, Bipasha Dey, Swati Sharma, and Richa Rikhy

Branched actin networks driven by Arp2/3 collaborate with actomyosin filaments in processes such as cell migration. The syncytial Drosophila blastoderm embryo also shows expansion of apical caps by Arp2/3 driven actin polymerization in interphase and buckling at contact edges by MyosinII to form furrows in metaphase. Here we study the role of Syndapin (Synd), an F-BAR domain containing protein in apical cap remodelling prior to furrow extension. synd depletion showed larger apical caps. STED super-resolution and TIRF microscopy showed long apical actin protrusions in caps in interphase and short protrusions in metaphase in control embryos. synd depletion led to sustained long protrusions even in metaphase. Loss of Arp2/3 function in synd mutants partly reverted defects in apical cap expansion and protrusion remodelling. MyosinII levels were decreased in synd mutants and MyosinII mutant embryos have been previously reported to have expanded caps. We propose that Syndapin function limits branching activity during cap expansion and affects MyosinII distribution in order to shift actin remodeling from apical cap expansion to favor lateral furrow extension.

The methods for X-ray crystal orientation are rapidly evolving towards versatility, fewer goniometry measurements, automation, high accuracy and precision. One method that attracts a lot of attention is energy-dispersive X-ray diffraction (EDXRD) which is based on detecting reflections from crystallographic planes in a crystal at fixed angles of a parallel polychromatic X-ray incident beam. In theory, an EDXRD peak can move in a diffraction pattern as a function of a crystallographic plane d-spacing and its orientation relative to a fixed direction in space can change also. This is equivalent to the possibility of measuring the orientation of single crystals. The article provides a modeling for the EDXRD method whose main feature is the nonmoving crystal in the sense of traditional goniometry where the angle measurements of diffracting planes are a must. The article defines the equation of orientation for the method and shows the derivation in great detail. It is shown that the exact solutions of the equations can be obtained using the generalized reduced gradient method, a mathematical subroutine that is implemented in Excel software. The significance and scientific impact of the work are discussed along with the validated tested results.

Image modeling for biomedical organs

Home remodeling can be done for function or just because the house needs a new look, or a combination of the two.

Do you know how many hours a week you spend in your bathroom? As a homeowner, the bathroom is one of the most used rooms in a house. Wouldn't you rather spend this time in a room that is not only functional but aesthetically pleasing as well?

Velvet is back and it's better than ever

American Home Improvements posts web article based on 60 years of remodeling experience; explains "Seven Common Mistakes to Avoid on Any Remodeling Project"

San Diego Company Has Earned a Spot Among Nation's Top Remodelers Every Year Since 2004

Art Deco takes the spotlight from Mid-Century Modern

Regional Winners Move on to the National Competition and will be Announced during the 2019 NARI Awards Reception April 12 in San Diego

Presented during the 2019 Kitchen and Bath Industry Show, the NKBA Award Follows a First Place ASID Award and Bath of the Year Distinction

San Diego Architecture and Design Firm is the Only San Diego Company to Receive a 2019 National CotY Award

JDR Earns a Rarely Bestowed Decade of Excellence Award, a National Award for a Bold, Mondrian-Inspired Whole Home, and a Regional Award for a Colorful Historic Home

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It's back to the future in 2020 design with pastels, softer shapes, rattan and retro-inspired geometrics

Regional Winners Advance to the National Competition and will be Announced during the 2020 NARI Awards Reception April 24 in St. Pete, Florida

Meet the Architects and Designers Responsible for Some of San Diego's Most Enviable Homes

Roads are important ecological features of forest landscapes, but their cause-andeffect relationships with other ecosystem components are only recently becoming included in integrated landscape analyses. Simulation models can help us to understand how forested landscapes respond over time to disturbance and socioeconomic factors, and potentially to address the important role roads play in these processes.

In the information overload age, the user needs to find... more

The importance to society of environmental services, provided by forest ecosystems, has significantly increased during the last few decades. A growing global concern with the deterioration of forests, beginning perhaps most noticeably in the 1980s, has led to an increasing public awareness of the environmental, cultural, economic, and social values that forests provide. Around the world, ideas of sustainable, close-to-nature, and multi-functional forestry have progressively replaced the older perception of forests as only a source for timber. The international impetus to protect and sustainably manage forests has come from global initiatives at management, conservation, and sustainable development related to all types of forests and forestry. A few of the more notable initiatives include: the 1992 Earth Summit in Rio de Janeiro, Brazil (United Nations Conference on Environment and Development, UNCED); regional follow-ups to the Earth Summit such as the Montreal Process and Helsinki Accords; the forest elements of the Convention on Biological Diversity (CBD); and the Framework Convention on Climate Change (FCCC).

Development of a regional lichen gradient model from community data is a powerful tool to derive lichen indexes of response to environmental factors for large-scale and long-term monitoring of forest ecosystems. The Forest Inventory and Analysis (FIA) Program of the U.S. Department of Agriculture Forest Service includes lichens in its national inventory of forests of the United States, to help monitor the status of forested ecosystems.

This report is a compilation of six briefing papers based on literature reviews and syntheses, prepared for U.S. Department of Agriculture, Forest Service policy analysts and decisionmakers about specific questions pertaining to climate change.