Conjugation is the process by which plasmids, including those that carry antibiotic-resistance genes, are mobilized from one bacterium (the donor) to another (the recipient). The conjugation efficiency of IncF-like plasmids relies on the formation of mating-pair stabilization via intimate interactions between outer membrane proteins on the donor (a plasmid-encoded TraN isoform) and recipient bacteria. Conjugation of the R100-1 plasmid into Escherichia coli and Klebsiella pneumoniae (KP) recipients relies on pairing between the plasmid-encoded TraNα in the donor and OmpW in the recipient. Here, the crystal structure of K. pneumoniae OmpW (OmpWKP) is reported at 3.2 Å resolution. OmpWKP forms an eight-stranded β-barrel flanked by extracellular loops. The structures of E. coli OmpW (OmpWEC) and OmpWKP show high conservation despite sequence variability in the extracellular loops.

Brucella ovis is an etiologic agent of ovine epididymitis and brucellosis that causes global devastation in sheep, rams, goats, small ruminants and deer. There are no cost-effective methods for the worldwide eradication of ovine brucellosis. B. ovis and other protein targets from various Brucella species are currently in the pipeline for high-throughput structural analysis at the Seattle Structural Genomics Center for Infectious Disease (SSGCID), with the aim of identifying new therapeutic targets. Furthermore, the wealth of structures generated are effective tools for teaching scientific communication, structural science and biochemistry. One of these structures, B. ovis leucine-, isoleucine-, valine-, threonine- and alanine-binding protein (BoLBP), is a putative periplasmic amino acid-binding protein. BoLBP shares less than 29% sequence identity with any other structure in the Protein Data Bank. The production, crystallization and high-resolution structures of BoLBP are reported. BoLBP is a prototypical bacterial periplasmic amino acid-binding protein with the characteristic Venus flytrap topology of two globular domains encapsulating a large central cavity containing the peptide-binding region. The central cavity contains small molecules usurped from the crystallization milieu. The reported structures reveal the conformational flexibility of the central cavity in the absence of bound peptides. The structural similarity to other LBPs can be exploited to accelerate drug repurposing.

The spatial orientation of α lamellae in a metastable β-Ti matrix of Timetal LCB (Ti–6.8 Mo–4.5 Fe–1.5 Al in wt%) was examined and the orientation of the hexagonal close-packed α lattice in the α lamella was determined. For this purpose, a combination of methods of small-angle X-ray scattering, scanning electron microscopy and electron backscatter diffraction was used. The habit planes of α laths are close to {111}β, which corresponds to (1320)α in the hexagonal coordinate system of the α phase. The longest α lamella direction lies approximately along one of the 〈110〉β directions which are parallel to the specific habit plane. Taking into account the average lattice parameters of the β and α phases in aged conditions in Timetal LCB, it was possible to index all main axes and faces of an α lath not only in the cubic coordinate system of the parent β phase but also in the hexagonal system of the α phase.

Millicent Simmonds, Noah Jupe and Emily Blunt return in “A Quiet Place, Part II.”; Credit: Paramount Pictures

This content is from Southern California Public Radio. View the original story at SCPR.org.

Drew Arriola Sands, left, sings in the South Gate band Trap Girl at La Conxa, 2017.; Credit: Amina Cruz

Growing up, Drew Arriola-Sands' music was "too weird for the weird kids." Her first band couldn't even get a backyard gig, but since Sands transitioned in 2013, her current band, Trap Girl, have been at the center of an exploding queer hardcore scene in Los Angeles. 

NOTE: Trans Pride L.A. is taking place this weekend, Saturday June 17, at the Los Angeles LGBT Center. To hear a preview of the event with organizer Gina Bigham, listen to the extra audio on this post.

Sands is 28 now, but she's always been drawn to glamorous women with big hair. Her mirror is adorned with pictures of Ronnie Spector, Dolly Parton, and Jayne Mansfield. Wig idols, she calls them. Sands has a large collection of wigs, and even makes her own, but it all started 20 years ago.

"When I was a little kid, my mom always had short black hair," Sands remembers. "And then one day, getting ready for school, she walked out of the bathroom with a long, thick, black braid with a ribbon on it, and it freaked me out, because I never saw her with long hair. So I was like, 'That’s weird! What is it?'" She was eight years old. For weeks to come, Sands would lock herself in the bathroom and stare at the extension braid in it's clear, Avon box until her mother threw it away without warning. The seed had been planted, though.

Her love of singing came at an early age too. As a child, Sands would stand up on a chair while watching baseball with her father to sing the national anthem. Her mother would scold her for being loud and tell her that she could sing at a baseball game when she was older. At 11, her father put her in little league.

We look at a picture of young Drew in a baseball jersey. Sands was a chubby little kid, biting down a smile, and burying her hand in her mitt. "I was a 'catcher' even then," laughs Sands.

"I was told I was gay before I even knew I was gay, because people saw I was feminine, did things a little different, spoke a little different, a little more sensitive," says Sands. Bullying was a consistent part of her childhood, with no one incident standing out because there was always "80 more horrible ones," she says. But she found ways to cope through her hobbies.

Her father said if she wasn't going to play a sport, she had to play an instrument. The first instrument she started with in earnest was the guitar, before picking up bass and more. "Nirvana was still the biggest band in the world. Everyone at my junior high who played guitar learned how to play 'Rape Me' or 'Smells like Teen Spirit' as their first song" says Sands. The first song sands learned on guitar was Nirvana's "About a Girl," and the first album she bought was Hole's "Live Through This."

"One of my first jobs, actually, was making burnt cd’s for a guy who sold them at the alley, and he made me copy Trina cd’s, ten at a time. She had songs on there like 'Nasty Bitch,' things like that, and I just loved it! But it was like a guilty pleasure, 'cause I was still a rock kid."  - Drew Arriola-Sands

By her early twenties, she started her first real band, The Glitter Path; Sands describes it as something like Daniel Johnston, the schizophrenic outsider musician, mixed with Patsy Cline - extremely emotional, "lying across the road, ready to die type of music." It didn't fit in in the "very straight, very cis, surf rock-indie" backyard scene, says Sands. She can't remember the band playing more than two or three shows, anywhere, but she says she doesn't hold any grudges.

The Glitter Path's "Wear a Wig"

We look at another photo of Sands from her Glitter Path days. She points out the increasing number of women’s accessories she was wearing at the time. She was starting to feel a change coming.

"I was in a relationship in 2013 with an artist, but I was male presenting, and I had these feelings of identity and gender, and I expressed them to him, and he accepted them," Sands says,  "but didn’t know how to deal with me and I didn’t know how to deal with myself." Sands boyfriend broke up with her, and she reevaluated her emotional state. "My mental health was not going to get better if I did not come out [as a trans person]," she decided.

She had a much easier time dating after transitioning, and one chance hook-up set Sands down a new musical road.

"So this guy I was hooking up with at the time would play the Damned in the room while we were hooking up. I had a guitar in the room, and he didn’t know I played music and said, 'Do you play guitar?' I said, 'Yeah.' He said, 'Well, you should start a band, like the Damned, and play guitar. It’d be good, looking the way you do, and wear ball gowns.'” - Drew Arriola-Sands

Sands started Trap Girl, not as guitarist, but as lead singer, in 2014.

The early shows were backyard gigs in South Central. Songs like “Dead Men Don’t Rape” went over well, but Sands wasn’t out as a trans performer yet. Maybe people could read between the lines though, with a name like Trap Girl. Sands offers a few definitions for Trap Girls/Trap Queens (though she has never settled on just one).

• A woman who helps out a "trap lord," or drug dealer
• A very convincing transvestite
• A girl trapped in a man's body

Throughout 2015, Trap Girl built their following Downtown and on the Eastside, with Sands finally out as a trans artist.

Trap Girl live at Xicana PUNK Night

"I started this band alone," explains Sands. "I didn’t know any queer people, I didn’t know any trans people, I didn’t know who was gonna help this band. Who was gonna give us a shot? So, I was ready to defend this band, even though there was no one defend it from."

Rather, Trap Girl were embraced and found sisterhood in bands like Sister Mantos and Yaawn. In 2016, Sands took it a step further and organized the first annual Transgress Fest (at the Santa Ana LGBT Center), for trans performers. "We had people as young as twelve to people as old as sixty in the audience," she says. "We had a huge turnout. I never expected that."

Transgress Fest is coming back in November. In the meantime, Trap Girl are getting ready to release their second EP, "The Black Market." The title track grapples with the question of whether or not a trans person needs surgery.

"Being a woman doesn’t mean you have to look like a woman. I didn’t know any trans people at all before I transitioned, so automatically, my idea was to think that I needed to present as feminine to be accepted as a trans person, but little did I know, that that’s the last thing you need to be a trans person. Not all people can pass, and that’s ok." - Drew Arriola-Sands

Sands says the takeaway from "The Black Market" is not to risk your life with black market cosmetic procedures. "These girls are killing themselves to achieve their looks," says Sands. "They’re getting it offline [sic], off Craigslist. You know, they go to someone’s basement and get their ass injected with cement, and then they go home and get a blood clot in their lungs, and they die." "The Black Market" EP is due for release this summer.

Trap Girl is singer Drew Arriola-Sands, bassist Ibette Ortiz, drummer Jorge Reveles, and guitarist Estevan Moreno.

This content is from Southern California Public Radio. View the original story at SCPR.org.

Still of Emily Blunt and Noah Jupe in the film “A Quiet Place Part II.”; Credit: Paramount Pictures

Larry Mantle and KPCC film critics Lael Loewenstein, Christy Lemire and Charles Solomon review this weekend’s new movie releases on streaming and on demand platforms.

• "A Quiet Place Part II," in wide release

• "Cruella," in wide release & Disney+

• "Dog Gone Trouble," on Netflix

• "Pretty Guardian Sailor Moon Eternal The Movie," on Netflix June 3

• "Moby Doc," In Select Theaters (check your local listings); VOD (including FandangoNow, iTunes, Vudu); Laemmle’s Virtual Cinema June 4

• "Changing The Game," on Hulu June 1

• "Port Authority," Cinelounge Sunset Hollywood; Laemmle’s Virtual Cinema; Digital/VOD (including Vudu, Google Play, FandangoNow) June 1

• "Plan B," on Hulu

• "Borat Supplemental Reportings," on Amazon Prime Video

• "Dead Pigs," on Laemmle’s Virtual Cinema

Our FilmWeek critics have been curating personal lists of their favorite TV shows and movies to binge-watch during self-quarantine. You can see recommendations from each of the critics and where you can watch them here.

Guests:

Christy Lemire, film critic for KPCC, RogerEbert.com and co-host of the ‘Breakfast All Day’ podcast; she tweets @christylemire

Lael Loewenstein, film critic for KPCC and film columnist for the Santa Monica Daily Press; she tweets @LAELLO

Charles Solomon, film critic for KPCC, Animation Scoop and Animation Magazine

This content is from Southern California Public Radio. View the original story at SCPR.org.

Full Text:

On August 17, 2017, scientists made history with the first direct observation of a merger between two neutron stars. It was the first cosmic event detected in both gravitational waves and the entire spectrum of light, from gamma rays to radio emissions. The impact also created a kilonova -- a turbocharged explosion that instantly forged several hundred planets’ worth of gold and platinum. The observations provided the first compelling evidence that kilonovae produce large quantities of heavy metals, a finding long predicted by theory. Astronomers suspect that all of the gold and platinum on Earth formed as a result of ancient kilonovae created during neutron star collisions. Based on data from the 2017 event, first spotted by the Laser Interferometer Gravitational-wave Observatory (LIGO), astronomers began to adjust their assumptions of how a kilonova should appear to Earth-bound observers. A team of scientists reexamined data from a gamma-ray burst spotted in August 2016 and found new evidence for a kilonova that went unnoticed during the initial observations.

Image credit: NASA/ESA/E. Troja

Constellation said it is seeking guidance from regulators after FERC's decision about co-location, which had become a promising prospect for Big Tech's plans to quickly access large amounts of power for its AI expansion instead of waiting for years to connect to the grid.

After reaching out to all customers supplied with impacted chocolate products, Barry Callebaut can confirm, based on its internal investigation, that no affected chocolate products by the Salmonella-positive production lot in Wieze, Belgium, entered the retail food chain.

The snacks, now available in the C-Store channel, which were first announced at the 2023 NACS Show in Atlanta this October.

Taco Bell and Kellanova unite to create "cheeziest" menu items, featuring a Cheez-It cracker 16x bigger than the original.

The brand is taking its experience crafting flatbread crackers and applying it to a new format with mini toasts, debuting at Whole Foods Markets nationwide.

Bakery ingredient manufacturer Pak Group, through its North American brand, Bellarise, announces its new, certified organic, clean label and Non-GMO dough conditioner: Bellarise BellaSPONGE Organic.

Bakery ingredient manufacturer Pak Group, through its North American brand, Bellarise, has introduced Bellarise BellaSOFT Organic 1500 Plus.

Genio’s Uncured Pepperoni Pizza is handmade in Italy using ingredients such as locally grown tomatoes, buffalo mozzarella, hand torn Ligurian basil, and uncured pepperoni.

The mother-daughter company seeks to provide treats that bring people together.

The flavor will join the brand's existing lineup of Bold + Herby, Simply Stellar, Maui Monk, and Sweet & Sparky flavors.

The company has been manufacturing premium mozzarella sticks since it was founded in 2015.

The company is introducing PopTarts Crunchy Poppers and Pringles products at the roadside convenience stores.

The brioche can be cross-utilized throughout all dayparts from contemporary breakfast to savory dinner creations.

In the award-winning FX television drama The Bear, bread plays a crucial roll—namely, the hinged Italian rolls used in crafting Chef Carmen Berzatto’s famous beef sandwiches.

Some candies don’t fit neatly into one column or another. That’s okay; the “other” candies like marshmallows, spreads, interactive novelty items, and such seem to be fitting into consumers’ candy budgets just fine. 

This week's Fun Friday includes a Nutella and Betches book club partnership, Nerds at the Big Game, Pillsbury partnering on candles, a Cheez-It college tailgating food truck, and Haribo's float debut at Macy's Thanksgiving Day parade.

The announcement represents a significant step as part of Kellanova's Better Days Promise to advance sustainable and equitable access to food.

Cheez-It, Club crackers to be sold with regenerative wheat.

Stellar Snacks recently debuted grain-free pretzels crafted with superfood ingredients, now officially available at select grocery stores throughout the U.S.

Kellanova has rereleased a slew of seasonal products for autumn and Halloween.

Scalding remains a hidden danger lurking in homes across the United States, particularly impacting the vulnerable. The plumber can avoid the unintended consequences of simply lowering water temperatures.

Plumbing & Mechanical and PM Engineer Chief Editor Nicole Krawcke sits down with Legionella expert Dr. Janet Stout, PhD, to discuss best practice strategies in the plumbing industry for implementing risk assessments and Water Management Programs.

The code-writing organizations are currently finalizing the 2021 edition of the Plumbing Code, which may be adopted in your state or local jurisdiction by 2023. That is how far in advance hot subjects that are being discussed now wait before becoming law.

Casella has launched an enhanced version of its Vortex3 high flow pump, designed to simplify asbestos and air sampling.

The schedule of free-to-attend, accessible webinars cover Casella’s core areas of expertise, including workplace monitoring for noise, environmental boundary, hand and arm vibration, and asbestos sampling.

The highly anticipated Season 3 of FX’s “The Bear” is just days away, and Stella Artois is returning for its starring role at the dining table.

Lewis Cellars unveiled a new look, with a new logo and varietal updates that will help wine lovers discover Lewis, it says. Lewis also launched its first-ever wine club, Lewis Insiders, and a new website elevating the customer experience.

TV/Talk Show-Women's Health and Wellness

With the fourth and final season of The Umbrella Academy now streaming on Netflix, the Hargreeves siblings are once again on the brink of an apocalypse.

Rescheduled Date to be Announced

South Korean local government cancels international event with 30,000 participants from 57 countries, causing significant financial loss.

Dr. Nigel Anthony Sellars is an expert in American Labor History and American Popular Culture with 30 years of experience in his profession.

Dr. Bellak serves as an allergist on behalf of Allergy and Asthma Associates

Venkatachalam Garimella, PhD, is lauded for his contributions as the owner and chief executive officer of VGL LABS

Glenn R. Femminella channels years of expertise to his work within the Construction Industry

Key Housing is a best-in-class provider of short term housing throughout California from Sacramento to San Diego.

Antonella Dewell, MS, is a leading dietitian who excels in the dietary management of Irritable Bowel Syndrome

Isabella Hope Morin, MS, excels in nutrition and dietetics as the founder of Holistic Hope, LLC

Initiative Includes Enhanced Staffing and Training, More Personalized Service, Broader Selection, And Amazing Discounts and Deals

Phil Cannella lends years of expertise to his work with Crash Proof Retirement, LLC and Retirement Media, Inc.

Connor R. Casarella is noted for his work in private aviation and as the founder of Volare Aviation Services

Dr. Emmanuella Vernet is a distinguished software engineering expert with a decade of experience in the information technology industry

Renowned as a keynote speaker, trainer, and Forbes Contributor, Romanella founded 3SIXTY Management Services, LLC. With 40+ years in leadership, he served as CEO and Director at UniTek Global Services after a 36-year UPS career.