Leandro Castellanos, exportero de Independiente Santa Fe entre 2015 y 2023, dialogó con El Alargue de Caracol Radio sobre la gran final del fútbol colombiano. El hoy concejal de Bogotá se mostró confiado en los jugadores del equipo bogotano, advirtió qué errores no deben cometer, elogió a Andrés Mosquera Marmolejo y expresó su deseo de que el partido se defina en los 90 minutos.

La presidenta de Naturgas, Luz Murgas, habló de como se darán las medidas que se implementarán para asegurar el cubrimiento de la demanda de gas.

El gobierno del alcalde Jaume Collboni está trazando un muy complicado plan para blindar el distrito de Ciutat Vella contra los monocultivos comerciales. via Pocket

Ella Hagen won her second Class 4A cross country crown on Saturday at the Norris Penrose Event Center.

Putting humans on Mars has been one of NASA’s driving missions for years, but they are still in the early stages of deciding what exactly that mission architecture will look like. One major factor is where to get the propellant to send the astronauts back to Earth. Advocates of space exploration often suggest harvesting the …

Continue reading "Scaling Propellant Production on Mars is Hard"

The post Scaling Propellant Production on Mars is Hard appeared first on Universe Today.

Eventually, every stellar civilization will have to migrate to a different star. The habitable zone around all stars changes as they age. If long-lived technological civilizations are even plausible in our Universe, migration will be necessary, eventually. Could Extraterrestrial Intelligences (ETIs) use stars themselves as stellar engines in their migrations? In broad terms, a stellar …

Continue reading "A Spider Stellar Engine Could Move Binary Stars Halfway Across a Galaxy"

The post A Spider Stellar Engine Could Move Binary Stars Halfway Across a Galaxy appeared first on Universe Today.

Removed From Troubles – Problem Resolved (Not open for application): Gothic Subculture (Lifestyles); Goths (People) Troubled People Miscellany (Not open for application) The following fanlistings are scheduled for removal on November 13, 2024. If your fanlisting is listed below, please fix the problem/send in a finished form and reply to the troubles email! Pending Removal: […]

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Virgin Atlantic and British Airways have announced further suspension of routes

As Chris Billam-Smith's unification bout against Gilberto Ramirez nears, BBC Sport speaks to those closest to him.

Elizabeth line, Great Western Railway and Heathrow Express services are all being affected.

The bacterial enhancer-binding protein (bEBP) FlrC, controls motility and colonization of Vibrio cholerae by regulating the transcription of class-III flagellar genes in σ54-dependent manner. However, the mechanism by which FlrC regulates transcription is not fully elucidated. Although, most bEBPs require nucleotides to stimulate the oligomerization necessary for function, our previous study showed that the central domain of FlrC (FlrCC) forms heptamer in a nucleotide-independent manner. Furthermore, heptameric FlrCC binds ATP in “cis-mediated” style without any contribution from sensor I motif 285REDXXYR291 of the trans protomer. This atypical ATP binding raises the question of whether heptamerization of FlrC is solely required for transcription regulation, or if it is also critical for ATPase activity. ATPase assays and size exclusion chromatography of the trans-variants FlrCC-Y290A and FlrCC-R291A showed destabilization of heptameric assembly with concomitant abrogation of ATPase activity. Crystal structures showed that in the cis-variant FlrCC-R349A drastic shift of Walker A encroached ATP-binding site, whereas the site remained occupied by ADP in FlrCC-Y290A. We postulated that FlrCC heptamerizes through concentration-dependent cooperativity for maximal ATPase activity and upon heptamerization, packing of trans-acting Tyr290 against cis-acting Arg349 compels Arg349 to maintain proper conformation of Walker A. Finally, a Trp quenching study revealed binding of cyclic-di-GMP with FlrCC. Excess cyclic-di-GMP repressed ATPase activity of FlrCC through destabilization of heptameric assembly, especially at low concentration of protein. Systematic phylogenetic analysis allowed us to propose similar regulatory mechanisms for FlrCs of several Vibrio species and a set of monotrichous Gram-negative bacteria.

Visual Abstract

Laura F Fielden
Nov 11, 2020; 0:RA120.002370v1-mcp.RA120.002370
Research

Modulation of the host cell is integral to the survival and replication of microbial pathogens. Several intracellular bacterial pathogens deliver bacterial proteins, termed ‘effector proteins’ into the host cell during infection by sophisticated protein translocation systems, which manipulate cellular processes and functions. The functional contribution of individual effectors is poorly characterised, particularly in intracellular bacterial pathogens with large effector protein repertoires. Technical caveats have limited the capacity to study these proteins during a native infection, with many effector proteins having only been demonstrated to be translocated during over-expression of tagged versions. Here we developed a novel strategy to examine effector proteins in the context of infection. We coupled a broad, unbiased proteomics-based screen with organelle purification to study the host-pathogen interactions occurring between the host cell mitochondrion and the Gram-negative, Q fever pathogen Coxiella burnetii. We identify 4 novel mitochondrially-targeted C. burnetii effector proteins, renamed Mitochondrial Coxiella effector protein (Mce) B to E. Examination of the subcellular localisation of ectopically expressed proteins confirmed their mitochondrial localisation, demonstrating the robustness of our approach. Subsequent biochemical analysis and affinity enrichment proteomics of one of these effector proteins, MceC, revealed the protein localises to the inner membrane and can interact with components of the mitochondrial quality control machinery. Our study adapts high-sensitivity proteomics to study intracellular host-pathogen interactions, providing a robust strategy to examine the sub-cellular localisation of effector proteins during native infection. This approach could be applied to a range of pathogens and host cell compartments to provide a rich map of effector dynamics throughout infection.

About 1,100 workers will be laid off indefinitely in January from the Jeep Gladiator plant in Toledo, Ohio, Stellantis officials announced Wednesday.

SpaceX successfully launched Wednesday a new batch of Starlink satellites into orbit to further its mission to expand Internet access to the global population in now more than 200 space missions.

The director of the Lemelson Center for the Study of Invention and Innovation explores how personality and environment help creativity flourish

Discover how Ella Jenkins' joyful songs and storytelling have inspired generations of young listeners, while her commitment to advocacy has profoundly affected the world of music and beyond. --------- For more videos from Smithsonian Magazine: https://www.smithsonianmag.com/videos/ Digital Editorial Director: Brian Wolly Director of Programming: Nicki Marko Supervising Producer & Scriptwriter: Michelle Mehrtens Producer & Editor: Sierra Theobald Motion Designer: Ricardo Jaimes

For years, the owner's son had wondered about the artwork, which features the Spanish painter's signature. Now, some experts think it's the real deal

The entrepreneur and financial adviser defeated Adam Bassett for the party nomination in Egmont after two days of voting last week. 

After COVID-19 challenges forced the cancellation of Canada's mixed doubles trials, several top curlers are anxiously waiting to learn whether they will be chosen to represent the country in the discipline at the Beijing Games.

Customers will be able to pick a flavor and a mix-in and walk off with fresh dessert in less than a minute

Scott Shellady, TJM Investments

Scott Shellady, TJM Investments

Scott Shellady, TJM Investments

Scott Shellady, TJM Investments

Scott Shellady, TJM Investments

Scott Shellady, TJM Investments

Scott Shellady, TJM Investments

Scott Shellady, TJM Investments

In late 2023, the Penn State Law in University Park Civil Rights Appellate Clinic — along with the Employment Law Group, a Washington, D.C.-based boutique litigation firm — filed merits briefs with the Ninth Circuit to appeal the district court’s dismissal of an ADA case. On the basis of this briefing and subsequent oral argument, the Ninth Circuit reversed the lower court’s ruling and remanded the case for trial.

Stellar Blade is getting a Nier: Automata DLC on November 20, developer Shift Up has announced. The game will get special items from the world of Nier Automata and an in-game shop as part of the collaboration. The update will also add a photo mode, new outfits and more features.

After eating Kalari Kulcha for the first time, Chef Sarah Todd could not help but call it her new favourite street food in India.

The Delaware Department of Agriculture (DDA) is warning anyone who purchased turtles, specifically the red-eared slider turtle, between August 2020 and January 2021 to take extra precautions to prevent illness.

The Delaware Division of Motor Vehicles (DMV) is excited to unveil the latest edition to their online services at dmv.de.gov, Della the Chatbot. Della began assisting customers on the website in early June 2024. DMV partnered with Google Cloud and SID Global Solutions Digital in the development of the AI-powered chatbot that will provide customers […]

Hello,

I am trying to design an RF mixer for a TX.
Assume my input IF signal is at 1 GHz, my LO at 2 GHz and I want my RF at 3 GHz, and assume that the mixer fully works after testing it with HB.
How to simply set the envelope analysis to check the EVM ?
I read through the documentation but I couldn't get it (I couldn't fully understand the settings of the source, probe, analysis.)
Which source and probe to use? I want custom modulation with custom bandwidth and center frequency.
Is there some examples for the ENVLP analysis ?

I am using IC23.1 and spectre 20.1.354.