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A one-pan Greek style rice with beef and eggplant in a tasty tomato sauce! Moussaka is a Greek dish typically containing layers of eggplant, a meat sauce, and a bechamel sauce and it takes a bit of time to make it. This dish is inspired by the flavours of moussaka as a quick and easy...

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The post Greek Style Beef and Eggplant Rice (aka Moussaka Rice) appeared first on Closet Cooking.

“Me being 6 feet 7 inches, I’m not your normal height-weight type cyclist. But that motivates me to get better, to be better, to go against these guys.”

Companies have continued to cut jobs in 2024 after a wave of layoffs last year. Meta, Goldman Sachs, Google, and Tesla are among those shedding staff.

The 2024 Egg Fair in Mexico broke a Guinness World Record when more than 2,000 young people participated in a race while balancing eggs on spoons.

Film star Chris Pratt and his wife, author Katherine Schwarzenegger, have announced the birth of their third child.

In this ode to improvisation, musician and comedian Reggie Watts beatboxes, raps, loops his own rhythms and reflects upon the everyday power of turning the mundane into magic. After all, he says, we're all just making it up as we go along.

A chick in the process of hatching as the female adult looks on

The newly discovered Lllacme plenipes has up to 750 legs, more than any other known creature

Milagra, Spanish for “miracle,” was hatched and raised in captivity by foster condor parents after her mother died of avian flu in April 2023

Laboratory study of natural populations of mosquitoes can play a key role in determining the underlying causes of variation in burdens of mosquito-borne disease. Aedes aegypti is the main vector of the viruses that cause dengue, chikungunya, Zika, and yellow fever, making it a high priority for laboratory study. Ae. aegypti eggs provide an ideal starting point for new laboratory colonies. Eggs can be collected using ovicups, which are small plastic cups lined with seed-germination paper and partially filled with leaf-infused H₂O. Once collected, dry eggs will remain viable for months and can be safely transported long distances back to the laboratory as long as they are properly stored. This protocol provides step-by-step instructions for preparing for collecting, storing, and hatching Ae. aegypti eggs and has successfully yielded laboratory colonies from locations across both the native and invasive range of this species.

The insect eggshell is a multifunctional structure with several important roles, including generating an entry point for sperm via the micropyle before oviposition, serving as an oviposition substrate attachment surface, and functioning as a protective layer during embryo development. Eggshell proteins play major roles in eggshell tanning and hardening following oviposition and provide molecular cues that define dorsal–ventral axis formation. Precise eggshell formation during ovarian follicle maturation is critical for normal embryo development and the synthesis of a defective eggshell often gives rise to inviable embryos. Therefore, simple and accurate methods for identifying eggshell proteins will facilitate our understanding of the molecular pathways regulating eggshell formation and the mechanisms underlying normal embryo development. This protocol describes how to isolate and enrich eggshells from mature oocytes of Aedes aegypti mosquitoes and how to extract their eggshell proteins for liquid chromatography with tandem mass spectrometry (LC–MS/MS) proteomic analysis. Although this methodology was developed for studying mosquito eggshells, it may be applicable to eggs from a variety of insects. Mosquitoes are ideal model organisms for this study as their ovarian follicle development and eggshell formation are meticulously regulated by blood feeding and their follicles develop synchronously throughout oogenesis in a time-dependent manner.

In insects, oocyte resorption (oosorption) or follicular atresia is one of the key physiological processes and evolutionary strategies used to optimize reproductive fitness. Mosquitoes are ideal model organisms for studying egg maturation in arthropods, as their follicle development is initiated only following the ingestion of a blood meal, followed by a carefully orchestrated series of hormonally regulated events leading to egg maturation. A cohort of approximately 100 follicles per mosquito ovary begin developing synchronously. However, a significant fraction of follicles ultimately undergo apoptosis and oosorption, especially when available resources from the blood meal are limited. Therefore, simple, rapid, and reliable techniques to accurately evaluate follicular atresia are necessary to understand mechanisms underlying follicle development in insects. This protocol describes how to detect apoptotic follicle cells within the Aedes aegypti mosquito ovaries using a commercially available fluorescent-labeled inhibitor of caspases (FLICA). Caspases are key players in animal apoptosis. In this assay, the FLICA reagent enters the intracellular compartment of follicles in dissected mosquito ovaries and covalently binds to active caspases. The bound reagent remains within the cell and its fluorescent signal can be observed by confocal microscopy. Although this method was specifically developed for visualizing apoptotic ovarian follicles during Ae. aegypti mosquito egg development, it should be applicable to other mosquito tissues that undergo caspase-mediated program cell death in a time-dependent manner.

Anautogenous female mosquitoes, which ingest a blood meal from warm-blooded vertebrates to produce eggs, have become a valuable model organism for investigating signaling pathways and physiological processes that occur during egg development. Different molecular pathways tightly regulate the initiation of egg development and are governed by a balance among different insect hormones. Gravid (mature egg-carrying) females deposit fully developed eggs at the end of each gonotrophic cycle, which is defined as the time interval between the ingestion of a blood meal to oviposition. An intact eggshell protects the oocyte and embryo inside from external factors such as desiccation, physical damage, etc., and the various eggshell proteins are spatially and temporary deposited during oogenesis. Additionally, follicle resorption (oosorption) during blood meal–induced mosquito ovarian follicle development is an adapted physiological process that optimizes reproductive fitness. Mosquito oocytes grow and mature synchronously throughout oogenesis; however, during the later stages of oogenesis, some oocytes may undergo oosorption if sufficient nutrients are unavailable. This introduction highlights how mosquito egg development can be used to investigate follicular resorption and identify proteins involved in eggshell formation in Aedes aegypti mosquitoes.

After preaching in a church and serving the community, team members each receive an egg as a gift from a local woman.

Seeing an opportunity to talk about Jesus’ sacrifice on the cross, the staff at SonRise Café in Tokyo sells Easter eggs before Easter.

Chris Pratt and Katherine Schwarzenegger married in an intimate ceremony in June 2019

South Indian Egg Rice isn't just delicious; it's packed with protein and flavour. Eggs are a solid source of protein, and adding them to rice with spices just makes it a total win.

  Wilmington, Del. (May 3, 2024) – The Delaware Division of the Arts’ Mezzanine Gallery presents Gregg Silvis’ exhibition, “15,000 Dowels,” on view from May 3-31, 2024. Guests are invited to attend a Meet-the-Artist Reception on Friday, May 3 from 5:00-7:00 p.m. The title of the exhibition comes from the essential material in this body […]