Princeton chemists are expanding our understanding of the universe by identifying complex molecules in interstellar space.

Laurie joined the Princeton faculty in 1966 and transferred to emeritus status in 2000.

Lottery giant Scientific Games Digital has filed a protest against an online gaming permit application submitted by SBTech because the company doesn't list specific list which countries it does business with.

The post Copy of Online gaming company has business ties to Iran appeared first on Shark Tank.

Jason M. Allen's "Theatre D'Opera Spatial" won an art competition at the 2022 Colorado State Fair, but the work was subsequently denied copyright protection due to his use of AI software to generate it and his unwillingness to disclaim that contribution to the whole. — Read the rest

The post AI artist appeals denial of copyright protection appeared first on Boing Boing.

Jacek R. Wiśniewski
Dec 1, 2014; 13:3497-3506
Research

Defining discontinuous antigenic epitopes remains a substantial challenge, as exemplified by the case of lipid transfer polyproteins, which are common pollen allergens. Hydrogen/deuterium exchange monitored by NMR can be used to map epitopes onto folded protein surfaces, but only if the complex rapidly dissociates. Modifying the standard NMR-exchange measurement to detect substoichiometric complexes overcomes this time scale limitation and provides new insights into recognition of lipid transfer polyprotein by antibodies. In the future, this new and exciting development should see broad application to a range of tight macromolecular interactions.

Connexin (Cx) protein forms hemichannels and gap junctional channels, which play diverse and profound roles in human physiology and diseases. Gap junctions are arrays of intercellular channels formed by the docking of two hemichannels from adjacent cells. Each hexameric hemichannel contains the same or different Cx isoform. Although homomeric Cxs forms have been largely described functionally and structurally, the stoichiometry and arrangement of heteromeric Cx channels remain unknown. The latter, however, are widely expressed in human tissues and variation might have important implications on channel function. Investigating properties of heteromeric Cx channels is challenging considering the high number of potential subunit arrangements and stoichiometries, even when only combining two Cx isoforms. To tackle this problem, we engineered an HA tag onto Cx26 or Cx30 subunits and imaged hemichannels that were liganded by Fab-epitope antibody fragments via atomic force microscopy. For Cx26-HA/Cx30 or Cx30-HA/Cx26 heteromeric channels, the Fab-HA binding distribution was binomial with a maximum of three Fab-HA bound. Furthermore, imaged Cx26/Cx30-HA triple liganded by Fab-HA showed multiple arrangements that can be derived from the law of total probabilities. Atomic force microscopy imaging of ringlike structures of Cx26/Cx30-HA hemichannels confirmed these findings and also detected a polydisperse distribution of stoichiometries. Our results indicate a dominant subunit stoichiometry of 3Cx26:3Cx30 with the most abundant subunit arrangement of Cx26-Cx26-Cx30-Cx26-Cx30-Cx30. To our knowledge, this is the first time that the molecular architecture of heteromeric Cx channels has been revealed, thus providing the basis to explore the functional effect of these channels in biology.

New blood tests screen for colorectal cancer in a much less invasive way than colonoscopy, but deaths will increase if many people choose this alternative, a new study indicates.

A new analysis suggests that the piece was created by several artists working in the Italian Renaissance painter's studio—and that Botticelli himself may have worked on important details

The technique of visualizing axon pathways in the embryonic ventral nerve cord using antibody labeling has been fundamental to our understanding of the genetic and developmental mechanisms underlying nervous system wiring in Drosophila. High-resolution microscopic examination of the ventral nerve cord remains an essential component of many experiments in Drosophila developmental neuroscience. Although it is possible to examine the ventral nerve cord in intact whole-mount embryos, to collect the highest-quality images it is often useful to isolate the nervous system away from the other embryonic tissues through embryo dissection. This protocol describes methods for dissecting ventral nerve cords from Drosophila embryos that have been fixed and stained via immunofluorescence or horseradish peroxidase (HRP) immunohistochemistry. The process of making fine dissection needles for this purpose from electrolytically sharpened tungsten wire is also described. Dissected and mounted ventral nerve cords can be examined and imaged using a variety of microscopy techniques including differential interference contrast (DIC) optics, epifluorescence, or confocal microscopy.

Hi, have two designs and would like to copy paste one area of circuit from the old design to the new design, best way/approach and guidance please..

I want to make an opening in the solder mask right above a trace that is acting like a guard ring. Do I really need to go and buy the Allegro Productivity Toolbox add-on for using the Cross-Copy tool for a basic operation like that??

/F

One day, doctors might be able to 3D print copies of your organs in order to test a variety of drugs, thanks to a new technique that uses light and sound for rapid printing

Title: Virtual Colonoscopy Can Spot Cancers Outside Colon
Category: Health News
Created: 8/20/2010 2:10:00 PM
Last Editorial Review: 8/23/2010 12:00:00 AM

Title: Longer Colonoscopy Time May Cut Cancer Risk
Category: Health News
Created: 8/27/2015 12:00:00 AM
Last Editorial Review: 8/28/2015 12:00:00 AM

One day, doctors might be able to 3D print copies of your organs in order to test a variety of drugs, thanks to a new technique that uses light and sound for rapid printing

Star Wars & VELUX Galactic Night Collection Copyright: VELUX Group

One day, doctors might be able to 3D print copies of your organs in order to test a variety of drugs, thanks to a new technique that uses light and sound for rapid printing

Highlights: The EN-840T DBE endoscope, created by Fujifilm with Prof. Hironori Yamamoto, prioritizes safety and

New study investigates how convolutional neural networks (CNNs) can enhance nasal endoscopy accuracy and efficiency. The study focuses on overcoming diagnostic

Voice disorders (!--ref1--) are prevalent among various professionals, including teachers, singers, lawyers, politicians, doctors, bus conductors, actors,

Penguin Random House, the trade publisher, is adding language to the copyright pages of its books to prohibit the use of those books to train AI. The Bookseller reports that new books and reprints of older titles from the publisher will now include the statement, “No part of this book may be used or reproduced […]

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