The largest addition to the New South Wales national parks network in almost a decade is welcomed by traditional owners of the land, who say it is a monumental cultural milestone.

For the first time ever, environmental water is being pumped into the dry and extremely saline wetland at Lyrup Forest near Berri in the hope it will turn into a 'sea monkey soup' that will provide a food source for bird and marine life.

An audit reveals 30 South Australian buildings require urgent remedial work to deal with potentially flammable aluminium cladding but the State Government is refusing to identify which ones are at risk.

An Adelaide business owner says buildings residents are not being informed about dangers posed by potentially flammable aluminium cladding, as an audit reveals dozens of structures are at risk.

Hospital buildings where flammable cladding posed a major fire threat to the public in Sydney and across the state are identified in NSW Health safety documents, with Sydney's St Vincent's Hospital the only one where potentially combustible panels still need to be removed.

Whether it's poor merging or illegally remaining in the overtaking lane, drivers who break the law and behave badly are contributing to road congestion in Queensland, experts say.

Grant personally responded to a video of the boy recreating his character Phoenix Buchanan's musical number from the film.

There’s more trouble for the team behind the popular HGTV series “Windy City Rehab.” Though Season 2 is expected to premiere later this year, the TV stars face multiple lawsuits, and they are starting to turn on each other.

There are a heck of a lot of reasons not to.

If you are just starting up your website, perhaps you are selling tangible items, or you have a hit single that you’ll be offering digital copies of, one of your pressing questions may be: “Which payment gateway should I use to accept payments from my customers?“. While there are pros and cons of using any […]

The post Benefits of Adding Multiple Payment Gateways to Your Website’s Checkout appeared first on Tips and Tricks HQ.

tRNAs universally carry a CCA nucleotide triplet at their 3'-ends. In eukaryotes, the CCA is added post-transcriptionally by the CCA-adding enzyme (CAE). The mitochondrion of the parasitic protozoan Trypanosoma brucei lacks tRNA genes and therefore imports all of its tRNAs from the cytosol. This has generated interest in the tRNA modifications and their distribution in this organism, including how CCA is added to tRNAs. Here, using a BLAST search for genes encoding putative CAE proteins in T. brucei, we identified a single ORF, Tb927.9.8780, as a potential candidate. Knockdown of this putative protein, termed TbCAE, resulted in the accumulation of truncated tRNAs, abolished translation, and inhibited both total and mitochondrial CCA-adding activities, indicating that TbCAE is located both in the cytosol and mitochondrion. However, mitochondrially localized tRNAs were much less affected by the TbCAE ablation than the other tRNAs. Complementation assays revealed that the N-terminal 10 amino acids of TbCAE are dispensable for its activity and mitochondrial localization and that deletion of 10 further amino acids abolishes both. A growth arrest caused by the TbCAE knockdown was rescued by the expression of the cytosolic isoform of yeast CAE, even though it was not imported into mitochondria. This finding indicated that the yeast enzyme complements the essential function of TbCAE by adding CCA to the primary tRNA transcripts. Of note, ablation of the mitochondrial TbCAE activity, which likely has a repair function, only marginally affected growth.

Researcher: Norbert Stoop, MIT
Title: Adding a New Wrinkle
Description: Norbert Stoop talks about new research on the formation of wrinkles.

Claude Cibils, Marcelo Lanzilotta, Eduardo N. Marcos and Andrea Solotar
Proc. Amer. Math. Soc. 148 (2020), 2421-2432.
Abstract, references and article information

tRNAs universally carry a CCA nucleotide triplet at their 3'-ends. In eukaryotes, the CCA is added post-transcriptionally by the CCA-adding enzyme (CAE). The mitochondrion of the parasitic protozoan Trypanosoma brucei lacks tRNA genes and therefore imports all of its tRNAs from the cytosol. This has generated interest in the tRNA modifications and their distribution in this organism, including how CCA is added to tRNAs. Here, using a BLAST search for genes encoding putative CAE proteins in T. brucei, we identified a single ORF, Tb927.9.8780, as a potential candidate. Knockdown of this putative protein, termed TbCAE, resulted in the accumulation of truncated tRNAs, abolished translation, and inhibited both total and mitochondrial CCA-adding activities, indicating that TbCAE is located both in the cytosol and mitochondrion. However, mitochondrially localized tRNAs were much less affected by the TbCAE ablation than the other tRNAs. Complementation assays revealed that the N-terminal 10 amino acids of TbCAE are dispensable for its activity and mitochondrial localization and that deletion of 10 further amino acids abolishes both. A growth arrest caused by the TbCAE knockdown was rescued by the expression of the cytosolic isoform of yeast CAE, even though it was not imported into mitochondria. This finding indicated that the yeast enzyme complements the essential function of TbCAE by adding CCA to the primary tRNA transcripts. Of note, ablation of the mitochondrial TbCAE activity, which likely has a repair function, only marginally affected growth.

Quebecers, like the rest of the world, are growing accustomed to the uncertainty that's accompanied the pandemic. But they may not appreciate their government adding to that already hefty burden.

Google has confirmed that it will introduce the ability to make group calls via Google Duo on the Web, starting as a preview on Chrome, in the coming weeks.

Businesses owned by individuals with disabilities and certain small businesses will now be included in the state’s supplier diversity efforts thanks to an executive order recently signed by Governor Jack Markell.

FTE workers/employees are entitled for all the benefits (except retrenchment compensation as termination of service as a result of completion of the tenure of FTE would not be considered as retrenchment) which are available to permanent employees.

The US administration has a plan of allowing 30,000 overseas workmen temporarily. They will help to carry out seasonal work in the country at the end of September. This reflects that the booming economy has complicated Presidential efforts in restricting…

The Bucs have never had more than four prime-time games in a year, but in 2020 they'll face the Bears, Raiders, Giants, Saints and Rams in prime time.

Question for forum:

I’m currently working on a code to automatically add CLP files to DRA files and then add two classes called “APPROVED” and “CLP”. To do this manually you have to open a DRA file, click file import subdrawing and choose the clp file with the same name as dra. (path already set). You then set the clp to position x 0 0. And then click on Set Up > Subclasses > Package geometry and type in “Approved” and “Clp.”

So far we’ve recorded the macros in Allegro for all of these actions. The macros correspond to one specific file name and we want to apply this to numerous files. To do this we created a python program that locates all of the specified CLP and DRA files, and if they have a matching name, runs a for loop that puts each file name into a stored variable that runs a loop for each file. We converted this script into batch and then added a function that we thought would run Allegro macros from batch.

In order to get the script working, we need to have an allegro batch command that will run the script without opening the Allegro start popup, or closing the popup when it appears.  We need to do this to run any script from starting Allegro.

I’ve done another similar program in batch where I made a for loop for each dra file and within the loop there was a batch a2dxf command that converted all dra files to dxf files. Is there a similar batch command for adding clp files to position 0 0 and/ or adding classes? If anyone has done something similar please let me know!

Thank you very much for the help.

Jen

Check here each week to keep up with the latest from John MacArthur's pulpit at Grace Community Church.

Check here each week to keep up with the latest from John MacArthur's pulpit at Grace Community Church.

Title: Adding Surgery to Meds May Improve Life With Parkinson's
Category: Health News
Created: 4/29/2010 8:10:00 AM
Last Editorial Review: 4/29/2010 12:00:00 AM

Season 5 of Respawn Entertainment’s hero-based battle royale kicks off next week and it looks like they’re doing more than just blowing up the map. This new Apex Legends trailer shows off what appears to be a horde mode PvE quest initiated by the newest Legend of the bunch: Loba. It looks like you’ll be […]

With the acquisition of a start-up specialising in encrypted messaging and cloud services, Zoom will finally be able to make good on its claims of offering end-to-end encryption.

Adding yoga to your regularly prescribed migraine treatment may be better than medication alone, according to a new study. The new research suggests yoga may help people with migraines have headaches that happen less often, don't last as long and are less painful.

Warming springs can cause plants to bloom earlier, alter hibernation times and locations for migrating animals, and increase insect populations

Across the south-eastern US, trees are unfurling their clouds of leaves after winter. Yet this picturesque and usually welcome development is this year cause for consternation.

New data from the USA National Phenology Network (USA-NPN) shows that in parts of North Carolina, South Carolina and northern Florida, spring has arrived more than three weeks earlier than average, and earlier than at any point in the last 39 years it has been tracked.

HIGH blood pressure is often referred to as the "silent killer" due to its non-obvious symptoms, which when they do appear are often too late. Adding this drink to your diet will lower your reading and reduce your risk of serious health complications.

Owners of apartments affected by the combustible cladding crisis in Victoria tell researchers of the emotional and financial toll it has taken on them and their neighbours.

Let's hope they play nicely with government-funded cargo bikes.

This modular curtain could help existing buildings photosynthesize oxygen and get rid of pollution in cities.

A Dutch landscape architecture firm is proposing a clever way to help animals navigate the urban environment -- using the same infrastructure human city-dwellers rely on to get from place to place.

If you’re to take composting to the next level, here’s a list of some less-common items that can also get tossed in your composting bin or tumbler.