I'm off to Glasgow, Scotland, later this week for Worldcon (the World Science Fiction Convention)! Very excited!!! Here's my full schedule. Hope to see you there! 

I had such a fantastic time at Worldcon 2024 in Glasgow!!!

The Dementia Services Development Centre (DSDC) draws on research and practice, from across the world, to provide a comprehensive, up-to-date resource on all aspects of dementia.

This episode is the audio only version of our live podcast with our friends from ArsenalVision. It was recorded live at Ally Pally Theatre on Saturday May 18th, the day before the final game of the season – so the discussion reflects that.

You can see a video version on YouTube here: https://youtu.be/UYlUR0pkqSo

Get extra bonus content and help support Arseblog by becoming an Arseblog Member on Patreon: https://www.patreon.com/arseblog

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In this episode of the Arsenal Women Arsecast, Tim is joined by ESPN’s Emily Keogh to compare notes on Arsenal Women’s tour of Washington DC. Emily and Tim were the only two UK based journalists to travel and they compare notes on the tour, the commercial and media benefits of it and Emily talks a little about interviews she conducted with Jonas Eidevall and Clare Wheatley in DC.

Get extra bonus content and help support Arseblog by becoming an Arseblog Member on Patreon: https://www.patreon.com/arseblog

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Welcome to the first episode of the Internet Musician Podcast! Show notes for Episode #1: Subscribe to the Internet Musician Podcast with iTunes: The first episode of The Internet Musician Podcast, hosted by indie artist and internet music marketing junkie Brian Hartzog (http://www.brianhartzog.net).  In this introductory episode, Brian introduces himself, his indie music credentials and […]

https://softwareengineeringdaily.com/2021/07/22/stack-overflow-for-teams-a-centralized-knowledge-sharing-and-collaboration-platform-with-tom-limoncelli/

If you've ever googled a CS or programming question, you likely found an answer (or many) on Stack Overflow. Founded in 2008 and named after a common computing error, Stack Overflow empowers the world to develop technology through collective knowledge. More than 100 million people visit Stack Overflow every month making it one of the 50 most-visited websites in the world. Stack Overflow's products include its market-leading knowledge sharing and collaboration platform, Stack Overflow for Teams, in addition to Stack Overflow Reach & Relevance, which is focused on advertising.

Stack Overflow for Teams is a knowledge sharing and collaboration solution that developers and managers already know and trust. It's for companies who need to increase productivity, decrease cycle times, accelerate time to market, and protect institutional knowledge. In this episode we talk with Tom Limoncelli, a manager at Stack Overflow, author, and tech advocate.

Listen to the podcast by clicking here!

Here are the honorable mentions from the 2024 Student Podcast Challenge. Congratulations!

We discuss the Scotland men’s squad, promoting young talent and Celtic v Chelsea.

velocityconf: Free webcast from our friends at @citrix 5/29 http://t.co/IOeY4U0wUP Learn to consolidate 40 load balancers and ADCs into single platform

TL;DR: Take 75% off this podcast player and discovery app with code TAKE10 at checkout.

We're not gonna lie: This app is so good it should come with a warning label. It's got everything a podcast junkie like yourself could ever want: millions of shows, easy discovery of new episodes, offline listening…But be warned, you'll only want more once you get a taste. — Read the rest

The post This app is your podcast dealer…don't get addicted appeared first on Boing Boing.

RecQ family helicases are highly conserved from bacteria to humans and have essential roles in maintaining genome stability. Mutations in three human RecQ helicases cause severe diseases with the main features of premature aging and cancer predisposition. Most RecQ helicases shared a conserved domain arrangement which comprises a helicase core, an RecQ C-terminal domain, and an auxiliary element helicase and RNaseD C-terminal (HRDC) domain, the functions of which are poorly understood. In this study, we systematically characterized the roles of the HRDC domain in E. coli RecQ in various DNA transactions by single-molecule FRET. We found that RecQ repetitively unwinds the 3'-partial duplex and fork DNA with a moderate processivity and periodically patrols on the ssDNA in the 5'-partial duplex by translocation. The HRDC domain significantly suppresses RecQ activities in the above transactions. In sharp contrast, the HRDC domain is essential for the deep and long-time unfolding of the G4 DNA structure by RecQ. Based on the observations that the HRDC domain dynamically switches between RecA core- and ssDNA-binding modes after RecQ association with DNA, we proposed a model to explain the modulation mechanism of the HRDC domain. Our findings not only provide new insights into the activities of RecQ on different substrates but also highlight the novel functions of the HRDC domain in DNA metabolisms.

Visual Abstract

Gα proteins promote dynamic adjustments of cell shape directed by actin-cytoskeleton reorganization via their respective RhoGEF effectors. For example, Gα13 binding to the RGS-homology (RH) domains of several RH-RhoGEFs allosterically activates these proteins, causing them to expose their catalytic Dbl-homology (DH)/pleckstrin-homology (PH) regions, which triggers downstream signals. However, whether additional Gα proteins might directly regulate the RH-RhoGEFs was not known. To explore this question, we first examined the morphological effects of expressing shortened RH-RhoGEF DH/PH constructs of p115RhoGEF/ARHGEF1, PDZ-RhoGEF (PRG)/ARHGEF11, and LARG/ARHGEF12. As expected, the three constructs promoted cell contraction and activated RhoA, known to be downstream of Gα13. Intriguingly, PRG DH/PH also induced filopodia-like cell protrusions and activated Cdc42. This pathway was stimulated by constitutively active Gαs (GαsQ227L), which enabled endogenous PRG to gain affinity for Cdc42. A chemogenetic approach revealed that signaling by Gs-coupled receptors, but not by those coupled to Gi or Gq, enabled PRG to bind Cdc42. This receptor-dependent effect, as well as CREB phosphorylation, was blocked by a construct derived from the PRG:Gαs-binding region, PRG-linker. Active Gαs interacted with isolated PRG DH and PH domains and their linker. In addition, this construct interfered with GαsQ227L's ability to guide PRG's interaction with Cdc42. Endogenous Gs-coupled prostaglandin receptors stimulated PRG binding to membrane fractions and activated signaling to PKA, and this canonical endogenous pathway was attenuated by PRG-linker. Altogether, our results demonstrate that active Gαs can recognize PRG as a novel effector directing its DH/PH catalytic module to gain affinity for Cdc42.

The Discoidin, CUB, and LCCL domain-containing protein (DCBLD) family consists of two type-I transmembrane scaffolding receptors, DCBLD1 and DCBLD2, which play important roles in development and cancer. The nonreceptor tyrosine kinases FYN and ABL are known to drive phosphorylation of tyrosine residues in YXXP motifs within the intracellular domains of DCBLD family members, which leads to the recruitment of the Src homology 2 (SH2) domain of the adaptors CT10 regulator of kinase (CRK) and CRK-like (CRKL). We previously characterized the FYN- and ABL-driven phosphorylation of DCBLD family YXXP motifs. However, we have identified additional FYN- and ABL-dependent phosphorylation sites on DCBLD1 and DCBLD2. This suggests that beyond CRK and CRKL, additional DCBLD interactors may be regulated by FYN and ABL activity. Here, we report a quantitative proteomics approach in which we map the FYN- and ABL-regulated interactomes of DCBLD family members. We found FYN and ABL regulated the binding of several signaling molecules to DCBLD1 and DCBLD2, including members of the 14-3-3 family of adaptors. Biochemical investigation of the DCBLD2/14-3-3 interaction revealed ABL-induced binding of 14-3-3 family members directly to DCBLD2.

Glycopeptides in peptide or digested protein samples pose a number of analytical and bioinformatics challenges beyond those posed by unmodified peptides or peptides with smaller posttranslational modifications. Exact structural elucidation of glycans is generally beyond the capability of a single mass spectrometry experiment, so a reasonable level of identification for tandem mass spectrometry, taken by several glycopeptide software tools, is that of peptide sequence and glycan composition, meaning the number of monosaccharides of each distinct mass, for example HexNAc(2)Hex(5) rather than man5. Even at this level, however, glycopeptide analysis poses challenges:  finding glycopeptide spectra when they are a tiny fraction of the total spectra; assigning spectra with unanticipated glycans, not in the initial glycan database; and finding, scoring, and labeling diagnostic peaks in tandem mass spectra.  Here we discuss recent improvements to Byonic, a glycoproteomics search program, that address these three issues. Byonic now supports filtering spectra by m/z peaks, so that the user can limit attention to spectra with diagnostic peaks, for example, at least two out of three of 204.087 for HexNAc, 274.092 for NeuAc (with water loss), and 366.139 for HexNAc-Hex, all within a set mass tolerance, for example, ± 0.01 Daltons. Also new is glycan "wildcard" search, which allows an unspecified mass within a user-set mass range to be applied to N- or O-linked glycans and enables assignment of spectra with unanticipated glycans. Finally the next release of Byonic supports user-specified peak annotations from user-defined posttranslational modifications. We demonstrate the utility of these new software features by finding previously unrecognized glycopeptides in publicly available data, including glycosylated neuropeptides from rat brain.

The epidemic of sexually transmitted infections (STIs) in the United States appears to be cooling off after more than two decades, a new Centers for Disease Control and Prevention (CDC) study says.

About 2.25 million U.S. middle- and high-school students this year say they use tobacco products, which is 500,000 fewer than a year ago, the Centers for Disease Control said Thursday.

Slivered onions served on McDonald's Quarter Pounders and other menu items caused a recent E. coli outbreak, the U.S. Centers for Disease Control and Prevention announced Wednesday.

Kansas State football coach Chris Klieman talks about what the Wildcats did during the open week ahead of their home game against Arizona State.

In a twist befitting its pages, the satirical, anti-establishment publication that delivered laughs and hijinks to generations of young readers gets the respect it always deserved with a new museum exhibition

On this episode of Generation Anthropocene, we dive into one of the most underappreciated elements on the periodic table

Food is a focal point for understanding broader environmental problems. In this podcast, we learn how food buyers are influenced in surprising ways.

Designed in SOLIDWORKS software, this lowriding sport machine becomes nimble highrider on demand

Penn State Mont Alto recently hosted its 19th annual MedCamp, a weeklong day camp providing rising ninth and 10th graders with opportunities to learn about careers in health care and medical fields. Twenty area highschoolers from across Franklin, Adams, Cumberland and York counties were accepted into MedCamp 2024.

The first reviews of the New Mac mini, iMac, and MacBook Pro, are in — and surprisingly range from delight to strange cynicism. Plus there are yet more rumors of the next Apple Vision Pro, but you need not hold your breath.

Pamir Productions, formed in 1991, passionately uses all forms of media to spread the gospel to Afghans worldwide.

Por Nuestros Niños, an education-focused Spanish-language radio show and podcast, could help families navigate an uncertain return to school.

In the latest episode of Invent Penn State’s "Dare to Disrupt" podcast, host Ryan Newman interviews Peter Cocoziello, chairman, founder and CEO of Advance Realty Investors, a company specializing in real estate development, construction and property management.