The Princeton microbiologist has also created a non-infectious version of the COVID-19 virus that researchers can study safely.

Pioneering Indian Muslim feminist Rokeya Sakhawat Hossain (1880-1932) wrote speculative science fiction, manifestoes, radical reportage, and incisive essays that transformed her experience of enforced segregation into unique interventions against gender oppression everywhere. Her radical imagination links the realities of living in a British colony to the technological and scientific breakthroughs of her time, the effects of hauntingly pervasive systems of sexual domination, and collective dreams of the future, forging a visionary, experimental body of work. If her contemporary B. R. Ambedkar urged the “annihilation of caste,” Rokeya demands nothing less than the annihilation of sexism, with education as the primary instrument of this revolution. Her brilliant wit and creativity reflect profoundly on the complexities of undoing deep-seated gender supremacy and summon her readers to imagine hitherto undreamed freedoms.

Please join the BusinessToday Seminars Team as we present Mr. Fred Cooper, Senior Vice President of Strategic Partnerships at Toll Brothers. Toll Brothers (NYSE: TOL) is a ~$10 billion revenue and ~$15 billion market cap, Fortune 500 Company founded in 1967 and ranked the 4th largest U.S. home builder by revenues. Toll is also among the largest multifamily rental apartment developers, and land and community developers in the U.S. In addition, Toll is one of the nation’s largest urban high-rise/high-density condo and rental tower developers, with 50+ buildings and over 7,000 units completed.

In the digital age, content is king. But managing and distributing content efficiently across various channels can be a royal pain without the right tools. RSS Ground has made another giant leap for content automation by integrating first with IFTTT,  now Zapier and soon Pabbly Connect.  Thousands of third-party apps and services are now ready to enhance your content […]

The post Full Zapier Experience + Other Automation Platforms appeared first on RSSground.com.

We continue our updates and improvements based on your recent suggestions. This time, we have enhanced some interface options as well as Personal Feeds functionality. Please enjoy! Pagination has been added to the bottom of all RSS Ground lists (tables). We have also added an “attention seeker” bouncing effect for the Filters icon. It will […]

The post Another set of updates for personal feeds and more appeared first on RSSground.com.

The following article, EMBARRASSING: Big Election Misses by Never Trumpers and Others, was first published on The Black Sphere.

I love metaphorically rubbing my crotch in the faces of these smug Leftist pukes and know-it-alls after they get their asses handed to them.

Continue reading EMBARRASSING: Big Election Misses by Never Trumpers and Others ...

The following article, Democrats May Soon Need Another Beatdown, was first published on The Black Sphere.

If you think Democrats are soul-searching the bona fide ass-kicking they took in the 2024 election, then you don't know Democrats.

Continue reading Democrats May Soon Need Another Beatdown ...

Longtime Slashdot reader thephydes writes: The hearing will go ahead on November 13 at 11:30 ET (16:30 GMT). Apparently, it will "further pull back the curtain on secret UAP research programs conducted by the U.S. government, and undisclosed findings they have yielded," according to a House statement. It's driven by two republicans, Nancy Mace (R-S.C.) and Glenn Grothman (R-Wis.), who say: "Americans deserve to understand what the government has learned about UAP sightings, and the nature of any potential threats these phenomena pose. We can only ensure that understanding by providing consistent, systemic transparency. We look forward to hearing from expert witnesses on ways to shed more light and bring greater accountability to this issue." "Expert witnesses in the hearing will include Luis Elizondo, a decorated former counterintelligence officer who has claimed for years that the U.S. government is hiding knowledge of UAP, including materials recovered from crashed flying saucers," reports Space.com. "The House hearing will also include Tim Gallaudet, a retired U.S. Navy Rear Admiral who unidentified submersible objects, arguing that 'these underwater anomalies jeopardize US maritime security.'" "Other speakers at the hearing include journalist Michael Shellenberger, who has also claimed the U.S. government is hiding UFO crash retrieval programs, and former NASA Associate Administrator of Space Policy and Partnerships Michael Gold, who is a member of NASA's independent UAP study team."

Read more of this story at Slashdot.

(Nov. 11)  If you think it’s a problem that seven “swing” states get more attention from presidential campaigns than the other 43 states combined, don’t blame the Electoral College system. […]

The post Forty-three other states can make themselves important appeared first on Quin Hillyer.

McLaren’s Lando Norris explains why he wants to win the British Grand Prix more than any other race in Formula 1.

Stunning shots of gannets, goldfinch and grouse on show as the Bird Photographer of the Year award winners are announced

Ukraine has said that the toll from a Russian strike on the hometown of President Volodymyr Zelensky had risen to four people, identifying the dead as a mother and her three children, including a newborn.

Marilyn Chinitz has worked with celebs like Tom Cruise, Michael Douglas, and Wendy Williams. She has been happily married for 38 years.

The ARJ21 is now to be known as the C909. The renaming plays into COMAC's ambition to enter the global market.

Kenya's candidate for the top job at the African Union said on Saturday that if US president-elect Donald Trump does not want to work with Africa, the continent has "other friends".

Nintendo is cracking down on emulators of its hardware and media depicting their use. First Yuzu was taken down with a lawsuit, and now departs Ryujinx, a similar project that had sought to avoid the legal landmines Yuzu stepped on. — Read the rest

The post Another emulation project disappears amid Nintendo crackdown appeared first on Boing Boing.

Conservative activist Scott Presler, whose organization Early Vote Action helped swing Pennsylvania to the right in the general election, announced Monday he will be working to do the same in […]

The post Democrats Have Another Problem to Worry About as Man Who Helped Trump Win Has Eyes on Blue State appeared first on The Western Journal.

Now that there is a Republican majority in the next Congress, it’s time for the party to bare its internal fault lines. That is likely to take place Wednesday, according […]

The post Another House Speaker Battle? Mike Johnson's Position May Be at Risk as Conservative Anger Heats Up appeared first on The Western Journal.

Presented by the Middle East Forum on Nov. 18, 2022 Panel discussion with Daniel Pipes, Cynthia Farahat, and Jonathan Schanzer:

Originally published in Gatestone Institute on Nov. 25, 2022 By Cynthia Farahat Bahgat Saber, a member of the Muslim Brotherhood, operates from his New York apartment and often streams live videos from Times Square. During his multi-hour videos, Saber routinely incites terrorism, assassinations, kidnapping and torture in an extremely graphic manner. The calls for violence […]

By SADF – Nov. 30, 2022 Online conversation on The West, a New Haven for the ‘Muslim Brotherhood’s Planetary Nebula’? On November the 29th at 16H.00 (CET), SADF promoted an online conversation in the web platform StreamYard titled ‘The West, a New Haven for the ‘Muslim Brotherhood’s Planetary Nebula’?’ The conversation included the Member of the European Parliament (EPP, […]

By Walter Donway, Savvy Street originally published on Dec. 4, 2022 Book Review: The Secret Apparatus: The Muslim Brotherhood’s Industry of Death by Cynthia Farahat, Post Hill Press, Bombardier Books (2022) A grim irony of twentieth-century history (but, of course, also predating it) is that the most horrendous threats to humanity have simply been too monstrously evil […]

By Cynthia Farahat On Friday 27th, 2023 at 11:00 am EST, my sweet dear younger brother Amir Farahat passed away. Amir was the most loving father I have ever seen, a great brother, son, husband, and brother-in-law. My little Miró, my little prince, my soul is shattered by your loss. I will forever mourn your […]

Climate action and gender equality: Can we close the gap on one without the other? 8 March 2021 — 2:30PM TO 4:00PM Anonymous (not verified) 15 February 2021 Online

In partnership with the COP26 presidency, policymakers and experts examine the interrelationship between gender equality and climate action, and highlight innovative examples of policy and practice from around the world.

Marking International Women’s Day, this event organized by the COP26 presidency in partnership with Chatham House, will explore how gender equality and climate action go hand-in-hand. The agreement of the Gender Action Plan (GAP) at COP25 sent the message that the time for gender-responsive climate action is now.

This will be a unique opportunity to hear from policymakers and civil society leaders discussing whether enough is being done, as well as highlighting cutting-edge work around the world and suggesting what the future could hold.

The event will be livestreamed on this event page.

‘Hong Kong is now in the hands of its people – they cannot rely on others to stick up for them now.’ Expert comment sysadmin 29 June 2017

Kerry Brown on ‘one country, two systems’, the UK’s diminishing influence and the territory’s future, 20 years after the handover.

1 July marks the 20th anniversary of the transfer of Hong Kong’s sovereignty from Britain to China. Kerry Brown speaks with Jason Naselli about what the future holds for the territory.

How sustainable is the ‘one country, two systems’ framework? Will the arrangement last the full 50 years (until 2047) as originally envisioned?

It is questionable whether the arrangement that exists today was the one envisioned in 1997 when the handover happened. It was always a very abstract, flexible system, granting Hong Kong a high degree of autonomy, meaning it could maintain its capitalist system. Of course, in the lead up to 1997 all these things were broadly seen as being in Beijing’s interests to preserve.

But these days, the one thing that few said in 1997 has come to pass – the People’s Republic has maintained one-party rule as a political system, but become one of the world’s great economies. It has been so far a huge success.

Hong Kong therefore has diminished in importance over the years to the point that maintaining at least some semblance of one country, two systems is almost like an act of charity. It has been nibbled at, compromised and seems to grow weaker by the day. Most in Hong Kong would say there is a system: one country, one system. That’s the deal.

The central government’s deepening involvement in the territory’s politics is a subject of growing controversy in Hong Kong. Does the Chinese government need to alter its approach?

Not particularly. It doesn’t want to see Hong Kong fail as an economy. That doesn’t suit its interests at all. But nor does it want a truculent, disobedient polity that is meant to be part of its sovereign territory.

So it has increasingly set political parameters. Hong Kong can have its unique system – as long as it is obedient. And on the whole, that is the deal that all of the city’s chief executives until now have internally understood perfectly.

As part of the 1997 handover, the UK has ‘a continuing moral and political obligation’ to Hong Kong. How will this relationship play out as Brexit shifts Britain’s place in the world?

The Foreign Office offers a six-monthly report to Parliament, updating on how the handover deal is going. As the years go on, however, it becomes increasingly illusive how the UK has any real locus to say much about the situation on Hong Kong. It did say, rightly, that the detainment of one of the booksellers taken in in 2015 was a violation of the treaty because he was British. This was the strongest wording that has ever appeared from an official British source. But with dependence on creating a new kind of relationship with China now foremost in people’s minds because of Brexit and other economic pressures, it is not surprising that the priority increasingly lies elsewhere.

With direct management of Hong Kong gone, the UK was always going to be more and more irrelevant. That has happened. And in any case, relations with China have had to become more complex and multifaceted. Hong Kong was always the tail wagging the dog for the UK relations with China. Now there has been a rebalancing, the calculation always has to be how much unilaterally supporting Hong Kong will damage relations with Beijing. This has become an increasingly asymmetrical question: in a playoff, preserving links with Beijing will always prevail. That’s just the reality of the new world we are seeing come into being.

Hong Kong has played an important financial role for China over the past 20 years, but where will it fit as markets and financial institutions on the mainland mature?

It maintains is role as a major RMB hub, and as a finance centre. But it is surrounded by competition. Singapore, and Sydney, and other places in the region have RMB deals. Shanghai and Tianjin aspire to be portals for entry to the domestic Chinese market. Hong Kong every day has to think of new ways to maintain its relevance and beat back competition. So far, it has done well. But this is an issue it can never be complacent about.

What has been the most significant change in Hong Kong society since 1997?

The rising cultural and linguistic influence of the mainland on Hong Kong. Hong Kong has maintained its difference – but it has had to change. It is clear that Hong Kong is now in the hands of Hong Kongese – they cannot rely on others to stick up for them now. The culture, identity and future of the territory are in their hands. In that sense, they have autonomy.

Kadian Morgan was overwhelmed with grief as she leaned against a wall outside her gate on Jackson Lane, East Kingston, yesterday, tears streaming down her face. Her 19-year-old son, Kayshan 'Bem Bem' Smith, was lying in the morgue after being...

Abnormal changes of neuronal Tau protein, such as phosphorylation and aggregation, are considered hallmarks of cognitive deficits in Alzheimer's disease. Abnormal phosphorylation is thought to precede aggregation and therefore to promote aggregation, but the nature and extent of phosphorylation remain ill-defined. Tau contains ∼85 potential phosphorylation sites, which can be phosphorylated by various kinases because the unfolded structure of Tau makes them accessible. However, methodological limitations (e.g. in MS of phosphopeptides, or antibodies against phosphoepitopes) led to conflicting results regarding the extent of Tau phosphorylation in cells. Here we present results from a new approach based on native MS of intact Tau expressed in eukaryotic cells (Sf9). The extent of phosphorylation is heterogeneous, up to ∼20 phosphates per molecule distributed over 51 sites. The medium phosphorylated fraction Pm showed overall occupancies of ∼8 Pi (± 5) with a bell-shaped distribution; the highly phosphorylated fraction Ph had 14 Pi (± 6). The distribution of sites was highly asymmetric (with 71% of all P-sites in the C-terminal half of Tau). All sites were on Ser or Thr residues, but none were on Tyr. Other known posttranslational modifications were near or below our detection limit (e.g. acetylation, ubiquitination). These findings suggest that normal cellular Tau shows a remarkably high extent of phosphorylation, whereas other modifications are nearly absent. This implies that abnormal phosphorylations at certain sites may not affect the extent of phosphorylation significantly and do not represent hyperphosphorylation. By implication, the pathological aggregation of Tau is not likely a consequence of high phosphorylation.

Visual Abstract

Visual Abstract

The acyltransferase LCAT mediates FA esterification of plasma cholesterol. In vitro studies have shown that LCAT also FA-esterifies several oxysterols, but in vivo evidence is lacking. Here, we measured both free and FA-esterified forms of sterols in 206 healthy volunteers and 8 individuals with genetic LCAT deficiency, including familial LCAT deficiency (FLD) and fish-eye disease (FED). In the healthy volunteers, the mean values of the ester-to-total molar ratios of the following sterols varied: 4β-hydroxycholesterol (4βHC), 0.38; 5,6α-epoxycholesterol (5,6αEC), 0.46; 5,6β-epoxycholesterol (5,6βEC), 0.51; cholesterol, 0.70; cholestane-3β,5α,6β-triol (CT), 0.70; 7-ketocholesterol (7KC), 0.75; 24S-hydroxycholesterol (24SHC), 0.80; 25-hydroxycholesterol (25HC), 0.81; 27-hydroxycholesterol (27HC), 0.86; and 7α-hydroxycholesterol (7αHC), 0.89. In the individuals with LCAT deficiency, the plasma levels of the FA-esterified forms of cholesterol, 5,6αEC, 5,6βEC, CT, 7αHC, 7KC, 24SHC, 25HC, and 27HC, were significantly lower than those in the healthy volunteers. The individuals with FLD had significantly lower FA-esterified forms of 7αHC, 24SHC, and 27HC than those with FED. It is of note that, even in the three FLD individuals with negligible plasma cholesteryl ester, substantial amounts of the FA-esterified forms of 4βHC, 5,6αEC, 7αHC, 7KC, and 27HC were present. We conclude that LCAT has a major role in the FA esterification of many plasma oxysterols but contributes little to the FA esterification of 4βHC. Substantial FA esterification of 4βHC, 5,6αEC, 7αHC, 7KC, and 27HC is independent of LCAT.

Renal Cell Carcinoma (RCC) is one of the most commonly diagnosed cancers worldwide with research efforts dramatically improving understanding of the biology of the disease. To investigate the role of the immune system in treatment-naïve clear cell Renal Cell Carcinoma (ccRCC), we interrogated the immune infiltrate in patient-matched ccRCC tumor samples, benign normal adjacent tissue (NAT) and peripheral blood mononuclear cells (PBMCs isolated from whole blood, focusing our attention on the myeloid cell infiltrate. Using flow cytometric, MS, and ExCYT analysis, we discovered unique myeloid populations in PBMCs across patient samples. Furthermore, normal adjacent tissues and ccRCC tissues contained numerous myeloid populations with a unique signature for both tissues. Enrichment of the immune cell (CD45⁺) fraction and subsequent gene expression analysis revealed a number of myeloid-related genes that were differentially expressed. These data provide evidence, for the first time, of an immunosuppressive and pro-tumorigenic role of myeloid cells in early, clinically localized ccRCC. The identification of a number of immune proteins for therapeutic targeting provides a rationale for investigation into the potential efficacy of earlier intervention with single-agent or combination immunotherapy for ccRCC.

Withdrawn by Author.

Colorectal cancer (CRC) arises as the consequence of progressive changes from normal epithelial cells through polyp to tumor, and thus is an useful model for studying metabolic shift. In the present study, we studied the metabolomic profiles using high analyte specific gas chromatography/mass spectrometry (GC/MS) and liquid chromatography tandem mass spectrometry (LC/MS/MS) to attain a systems-level view of the shift in metabolism in cells progressing along the path to CRC. Colonic tissues including tumor, polyps and adjacent matched normal mucosa from 26 patients with sporadic CRC from freshly isolated resections were used for this study. The metabolic profiles were obtained using GC/MS and LC/MS/MS. Our data suggest there was a distinct profile change of a wide range of metabolites from mucosa to tumor tissues. Various amino acids and lipids in the polyps and tumors were elevated, suggesting higher energy needs for increased cellular proliferation. In contrast, significant depletion of glucose and inositol in polyps revealed that glycolysis may be critical in early tumorigenesis. In addition, the accumulation of hypoxanthine and xanthine, and the decrease of uric acid concentration, suggest that the purine biosynthesis pathway could have been substituted by the salvage pathway in CRC. Further, there was a step-wise reduction of deoxycholic acid concentration from mucosa to tumors. It appears that to gain a growth advantage, cancer cells may adopt alternate metabolic pathways in tumorigenesis and this flexibility allows them to adapt and thrive in harsh environment.

Oxidative stress has been implicated in aging and many human diseases, notably neurodegenerative disorders and various cancers. The reactive oxygen species that are generated by aerobic metabolism and environmental stressors can chemically modify proteins and alter their biological functions. Cells possess protein repair pathways to rescue oxidized proteins and restore their functions. If these repair processes fail, oxidized proteins may become cytotoxic. Cell homeostasis and viability are therefore dependent on the removal of oxidatively damaged proteins. Numerous studies have demonstrated that the proteasome plays a pivotal role in the selective recognition and degradation of oxidized proteins. Despite extensive research, oxidative stress-triggered regulation of proteasome complexes remains poorly defined. Better understanding of molecular mechanisms underlying proteasome function in response to oxidative stress will provide a basis for developing new strategies aimed at improving cell viability and recovery as well as attenuating oxidation-induced cytotoxicity associated with aging and disease. Here we highlight recent advances in the understanding of proteasome structure and function during oxidative stress and describe how cells cope with oxidative stress through proteasome-dependent degradation pathways.

Angola's Business Promise: Evaluating the Progress of Privatization and Other Economic Reforms 21 January 2020 — 2:30PM TO 3:30PM Anonymous (not verified) 16 January 2020 Chatham House | 10 St James's Square | London | SW1Y 4LE

Minister Nunes Júnior will discuss the progress of the Angolan government’s economic stabilization plans and business reform agenda including the privatization of some state-owned enterprises. These reforms could expand Angola’s exports beyond oil and stimulate new industries and more inclusive economic growth.

THIS EVENT IS NOW FULL AND REGISTRATION HAS CLOSED.

Radiolabeled small-molecule DOTA-haptens can be combined with antitumor/anti-DOTA bispecific antibodies (BsAbs) for pretargeted radioimmunotherapy (PRIT). For optimized delivery of the theranostic - and β-emitting isotope ¹⁷⁷Lu with DOTA-based PRIT (DOTA-PRIT), bivalent Gemini (DOTA-Bn-thiourea-PEG4-thiourea-Bn-DOTA, aka (3,6,9,12-tetraoxatetradecane-1,14-diyl)bis(DOTA-benzyl thiourea)) was developed. Methods: Gemini was synthesized by linking 2 S-2-(4-isothiocyanatobenzyl)-DOTA molecules together via a 1,14-diamino-PEG4 linker. [¹⁷⁷Lu]Lu-Gemini was prepared with no-carrier-added ¹⁷⁷LuCl₃ to a molar-specific activity of 123 GBq/μmol and radiochemical purity of more than 99%. The specificity of BsAb-¹⁷⁷Lu-Gemini was verified in vitro. Subsequently, we evaluated biodistribution and whole-body clearance for [¹⁷⁷Lu]Lu-Gemini and, for comparison, our gold-standard monovalent [¹⁷⁷Lu]Lu-S-2-(4-aminobenzyl)-DOTA ([¹⁷⁷Lu]Lu-DOTA-Bn) in naïve (tumor-free) athymic nude mice. For our proof-of-concept system, a 3-step pretargeting approach was performed with an established DOTA-PRIT regimen (anti-GPA33/anti-DOTA IgG-scFv BsAb, a clearing agent, and [¹⁷⁷Lu]Lu-Gemini) in mouse models. Results: Initial in vivo studies showed that [¹⁷⁷Lu]Lu-Gemini behaved similarly to [¹⁷⁷Lu]Lu-DOTA-Bn, with almost identical blood and whole-body clearance kinetics, as well as biodistribution and mouse kidney dosimetry. Pretargeting [¹⁷⁷Lu]Lu-Gemini to GPA33-expressing SW1222 human colorectal xenografts was highly effective, leading to absorbed doses of [¹⁷⁷Lu]Lu-Gemini for blood, tumor, liver, spleen, and kidneys of 3.99, 455, 6.93, 5.36, and 14.0 cGy/MBq, respectively. Tumor–to–normal tissue absorbed-dose ratios (i.e., therapeutic indices [TIs]) for the blood and kidneys were 114 and 33, respectively. In addition, we demonstrate that the use of bivalent [¹⁷⁷Lu]Lu-Gemini in DOTA-PRIT leads to improved TIs and augmented [¹⁷⁷Lu]Lu-Gemini tumor uptake and retention in comparison to monovalent [¹⁷⁷Lu]Lu-DOTA-Bn. Finally, we established efficacy in SW1222 tumor-bearing mice, demonstrating that a single injection of anti-GPA33 DOTA-PRIT with 44 MBq (1.2 mCi) of [¹⁷⁷Lu]Lu-Gemini (estimated tumor-absorbed dose, 200 Gy) induced complete responses in 5 of 5 animals and a histologic cure in 2 of 5 (40%) animals. Moreover, a significant increase in survival compared with nontreated controls was noted (maximum tolerated dose not reached). Conclusion: We have developed a bivalent DOTA-radiohapten, [¹⁷⁷Lu]Lu-Gemini, that showed improved radiopharmacology for DOTA-PRIT application. The use of bivalent [¹⁷⁷Lu]Lu-Gemini in DOTA-PRIT, as opposed to monovalent [¹⁷⁷Lu]Lu-DOTA-Bn, allows curative treatments with considerably less administered ¹⁷⁷Lu activity while still achieving high TIs for both the blood (>100) and the kidneys (>30).

New cross-species research suggests a theory of mind is one thing that sets humans apart from apes

President Joe Biden on Monday addressed a Veterans Day crowd at the national cemetery in Virginia where he revealed newly-expanded veteran benefits and urged Americans to "come together" and "keep faith."

SpaceX launched another batch of 23 Starlink V2 Mini satellites into low Earth orbit aboard a Falcon 9 rocket from Cape Canaveral Space Force Station Thursday at 3:19 p.m. EST.

Animal behavior expert Philip Johns introduces us to the vibrant urban environments of Singapore, where city dwellers and skyscrapers coexist with a rich array of other species, including otters, hornbills and lizards — prompting the question: Can we design cities to be wildlife refuges?

Lucille Bridges, the mother of civil rights activist Ruby Bridges, who walked with her then-6-year-old daughter past crowds screaming racist slurs as she became the first Black student at her all-white New Orleans elementary school, has died at the age of 86.

School districts are competing against each other for purchases of digital devices as remote learning expands to schools across the country.