You can download the official release of Adobe Flash Player 10.1 on the Adobe site.

This is the first step in what will be an extremely eventful year for streaming media. Adobe will soon enter the enterprise market in a big way with a version of Flash Media Server that supports both IP and Application Multicasting.

Flash has long dominated the public internet, but its inability to multicast meant corporations had to stick with Windows Media. This new capability will give large enterprises the opportunity to migrate to the Flash format and benefit from its ability to run on any OS and any browser, ease of use, support for higher quality codecs, ability to change bit rates on the fly etc.

Myosins generate force and motion by precisely coordinating their mechanical and chemical cycles, but the nature and timing of this coordination remains controversial. We utilized a FRET approach to examine the kinetics of structural changes in the force-generating lever arm in myosin V. We directly compared the FRET results with single-molecule mechanical events examined by optical trapping. We introduced a mutation (S217A) in the conserved switch I region of the active site to examine how myosin couples structural changes in the actin- and nucleotide-binding regions with force generation. Specifically, S217A enhanced the maximum rate of lever arm priming (recovery stroke) while slowing ATP hydrolysis, demonstrating that it uncouples these two steps. We determined that the mutation dramatically slows both actin-induced rotation of the lever arm (power stroke) and phosphate release (≥10-fold), whereas our simulations suggest that the maximum rate of both steps is unchanged by the mutation. Time-resolved FRET revealed that the structure of the pre– and post–power stroke conformations and mole fractions of these conformations were not altered by the mutation. Optical trapping results demonstrated that S217A does not dramatically alter unitary displacements or slow the working stroke rate constant, consistent with the mutation disrupting an actin-induced conformational change prior to the power stroke. We propose that communication between the actin- and nucleotide-binding regions of myosin assures a proper actin-binding interface and active site have formed before producing a power stroke. Variability in this coupling is likely crucial for mediating motor-based functions such as muscle contraction and intracellular transport.

Investigations of bacterial resistance strategies can aid in the development of new antimicrobial drugs as a countermeasure to the increasing worldwide prevalence of bacterial antibiotic resistance. One such strategy involves the TipA class of transcription factors, which constitute minimal autoregulated multidrug resistance (MDR) systems against diverse antibiotics. However, we have insufficient information regarding how antibiotic binding induces transcriptional activation to design molecules that could interfere with this process. To learn more, we determined the crystal structure of SkgA from Caulobacter crescentus as a representative TipA protein. We identified an unexpected spatial orientation and location of the antibiotic-binding TipAS effector domain in the apo state. We observed that the α6–α7 region of the TipAS domain, which is canonically responsible for forming the lid of antibiotic-binding cleft to tightly enclose the bound antibiotic, is involved in the dimeric interface and stabilized via interaction with the DNA-binding domain in the apo state. Further structural and biochemical analyses demonstrated that the unliganded TipAS domain sterically hinders promoter DNA binding but undergoes a remarkable conformational shift upon antibiotic binding to release this autoinhibition via a switch of its α6–α7 region. Hence, the promoters for MDR genes including tipA and RNA polymerases become available for transcription, enabling efficient antibiotic resistance. These insights into the molecular mechanism of activation of TipA proteins advance our understanding of TipA proteins, as well as bacterial MDR systems, and may provide important clues to block bacterial resistance.

The HIV-1 protein Gag assembles at the plasma membrane and drives virion budding, assisted by the cellular endosomal complex required for transport (ESCRT) proteins. Two ESCRT proteins, TSG101 and ALIX, bind to the Gag C-terminal p6 peptide. TSG101 binding is important for efficient HIV-1 release, but how ESCRTs contribute to the budding process and how their activity is coordinated with Gag assembly is poorly understood. Yeast, allowing genetic manipulation that is not easily available in human cells, has been used to characterize the cellular ESCRT function. Previous work reported Gag budding from yeast spheroplasts, but Gag release was ESCRT-independent. We developed a yeast model for ESCRT-dependent Gag release. We combined yeast genetics and Gag mutational analysis with Gag-ESCRT binding studies and the characterization of Gag-plasma membrane binding and Gag release. With our system, we identified a previously unknown interaction between ESCRT proteins and the Gag N-terminal protein region. Mutations in the Gag-plasma membrane–binding matrix domain that reduced Gag-ESCRT binding increased Gag-plasma membrane binding and Gag release. ESCRT knockout mutants showed that the release enhancement was an ESCRT-dependent effect. Similarly, matrix mutation enhanced Gag release from human HEK293 cells. Release enhancement partly depended on ALIX binding to p6, although binding site mutation did not impair WT Gag release. Accordingly, the relative affinity for matrix compared with p6 in GST-pulldown experiments was higher for ALIX than for TSG101. We suggest that a transient matrix-ESCRT interaction is replaced when Gag binds to the plasma membrane. This step may activate ESCRT proteins and thereby coordinate ESCRT function with virion assembly.

In SAS Business Rules Manager 3.3, the initial loading of a rule set and a rule flow takes significantly longer than it does in release 3.2. When this problem happens, long time gaps are evident in the local

When you log on to the SAS Risk Governance Framework and choose a solution, the web application might fail to load the solution content. When the problem occurs, you continue to see "Loading..." on the screen, an

In eukaryotic DNA replication, DNA polymerase ε (Polε) is responsible for leading strand synthesis, whereas DNA polymerases α and δ synthesize the lagging strand. The human Polε (hPolε) holoenzyme is comprised of the catalytic p261 subunit and the noncatalytic p59, p17, and p12 small subunits. So far, the contribution of the noncatalytic subunits to hPolε function is not well understood. Using pre-steady-state kinetic methods, we established a minimal kinetic mechanism for DNA polymerization and editing catalyzed by the hPolε holoenzyme. Compared with the 140-kDa N-terminal catalytic fragment of p261 (p261N), which we kinetically characterized in our earlier studies, the presence of the p261 C-terminal domain (p261C) and the three small subunits increased the DNA binding affinity and the base substitution fidelity. Although the small subunits enhanced correct nucleotide incorporation efficiency, there was a wide range of rate constants when incorporating a correct nucleotide over a single-base mismatch. Surprisingly, the 3'→5' exonuclease activity of the hPolε holoenzyme was significantly slower than that of p261N when editing both matched and mismatched DNA substrates. This suggests that the presence of p261C and the three small subunits regulates the 3'→5' exonuclease activity of the hPolε holoenzyme. Together, the 3'→5' exonuclease activity and the variable mismatch extension activity modulate the overall fidelity of the hPolε holoenzyme by up to 3 orders of magnitude. Thus, the presence of p261C and the three noncatalytic subunits optimizes the dual enzymatic activities of the catalytic p261 subunit and makes the hPolε holoenzyme an efficient and faithful replicative DNA polymerase.

MLCommons this week issued the results of its latest MLPerf Inference (v3.1) benchmark exercise. Nvidia was again the top performing accelerator, but Intel (Xeon CPU) and Habana (Gaudi1 and 2) […]

The post MLPerf Releases Latest Inference Results and New Storage Benchmark appeared first on HPCwire.

June 24, 2024 — Haiqu, a quantum computing startup, announced the release of its new open-source toolkit – Rivet. This new toolkit is made to help developers streamline the execution of […]

The post Haiqu Releases Open-Source Rivet for Quantum Workflow Execution appeared first on HPCwire.

SAN JOSE, Calif., Nov. 6, 2024 — Super Micro Computer, Inc. (Nasdaq: SMCI), a Total IT Solution Provider for AI, Cloud, Storage, and 5G/Edge, has provided an update from the independent […]

The post Supermicro Releases Preliminary Q1 Earnings Amid Ongoing Compliance Issues appeared first on HPCwire.

HSINCHU, Taiwan, Nov. 8, 2024 – TSMC (TWSE: 2330, NYSE: TSM) today announced its net revenue for October 2024. On a consolidated basis, revenue for October 2024 was approximately NT$314.24 […]

The post TSMC Releases October 2024 Revenue Report appeared first on HPCwire.

K-pop group Stray Kids released the Japanese album "Giant" and a music video for its song of the same name.

An unnamed astronaut was released from Ascension Sacred Heart Pensacola in Florida and returned to Johnson Space Center in Houston in good health Saturday, NASA reported.

Gov. Kate Brown ordered the public release of annual school performance ratings last week after Oregon's biggest newspaper reported that a Brown appointee had delayed the release of the statistical rankings until after the high-stakes gubernatorial election Nov. 6.

The second College Football Playoff rankings produced some positives and negatives for teams. Here are the winners and losers from Tuesday's reveal:

While Tuesday is exciting because college hoops fans get to take in the Champions Classic, the College Football Playoff results after this past week were released after the first of two basketball games on ESPN. Ohio State football, as expected, stay

Mitchell G. Spring
Oct 9, 2024; 44:e0602242024-e0602242024
BehavioralSystemsCognitive

Serotonin modulates diverse phenotypes and functions including depressive, aggressive, impulsive, and feeding behaviors, all of which have reward-related components. To date, research has focused on understanding these effects by measuring and manipulating dorsal raphe serotonin neurons and using single-receptor approaches. These studies have led to a better understanding of the heterogeneity of serotonin actions on behavior; however, they leave open many questions about the timing and location of serotonin's actions modulating the neural circuits that drive these behaviors. Recent advances in genetically encoded fluorescent biosensors, including the GPCR activation-based sensor for serotonin (GRAB-5-HT), enable the measurement of serotonin release in mice on a timescale compatible with a single rewarding event without corelease confounds. Given substantial evidence from slice electrophysiology experiments showing that serotonin influences neural activity of the striatal circuitry, and the known role of the dorsal medial striatal (DMS) in reward-directed behavior, we focused on understanding the parameters and timing that govern serotonin release in the DMS in the context of reward consumption, external reward value, internal state, and cued reward. Overall, we found that serotonin release is associated with each of these and encodes reward anticipation, value, approach, and consumption in the DMS.

Milagra, Spanish for “miracle,” was hatched and raised in captivity by foster condor parents after her mother died of avian flu in April 2023

Created in London in the 1960s, the tracks are heading to auction as part of a larger collection of memorabilia connected to the famous American guitarist

SOLIDWORKS Edition for Windows Vista delivers enhanced user experience plus advanced search, preview, and content distribution

On this episode of the HomeKit Insider Podcast we talk about Apple's new betas, discuss the major Matter 1.4 release which adds new categories and other improvements, and more!

An Apple supplier may have been tapped to build an Apple-designed smart home camera and future health-focused AirPods, according to analyst Ming-Chi Kuo.

iOS 18.2 with access to ChatGPT integrations, Image Playground, and Genmoji could finally roll out to the general public on December 9 based on a notice recently posted by a UK carrier.

A state court ruled that Virginia must turn over growth data by school and classroom teacher, without redacting the teachers' names.

DOVER, DE – Governor John Carney on Friday released the following statement on Director Eugene Young Jr.’s decision to step down March 8, 2024, from his current role as the head of the Delaware State Housing Authority. Director Young has served as the agency’s leader since 2021.   “Eugene is a strong leader dedicated to public service. […]

The mock test papers series-I will commence from November 18, 2024 while that for series-II will begin from December 9, 2024.

Allu Arjun, the star of Pushpa 2, will join Nandamuri Balakrishna on the iconic Telugu talk show ‘Unstoppable with NBK’. In this episode, airing November 15 on Aha, Arjun speaks about his acting inspirations, memorable co-stars, and personal insights shared by his mother. The episode will also preview Pushpa 2 and his historic National Award win.

Prashanth Neel’s Bagheera, starring Sriimurali as a vigilante IPS officer, comes to Netflix for streaming this December. Known for its compelling storyline and powerful cast, including Prakash Raj and Rukmini Vasanth, Bagheera captivated cinema-goers and emerged as a Diwali hit. Netflix’s acquisition marks a significant step for Kannada cinema on the platform, with Bagheera joining other Hombale Films projects. The exact streaming date remains unconfirmed, but fans can expect the movie in early December.

A Very Royal Scandal, featuring Ruth Wilson and Michael Sheen, brings viewers into the world of the British royal family’s explosive 2019 interview that revealed new truths about Prince Andrew. Now available in India on SonyLIV, the series dives deep into royal drama. Directed by Julian Jarrold and produced by Amazon MGM Studios, it revisits key events and figures surrounding one of the monarchy’s most notable scandals.

Google released a new experimental artificial intelligence (AI) tool on Monday aimed at helping users learn about new topics. Dubbed Learn About, the tool is different from the Mountain View-based tech giant's usual AI offerings, such as Gemini or AI Overviews. The experimental tool shows the information in a visual and interactive style and picks the data from educational sources.

Disney+ Hotstar's new Tamil series Parachute, directed by Sridhar K, features actors Shaam, Kishore, and Kani Thiru, focusing on a warm family dynamic. The teaser, now available on Disney+ Hotstar’s social media, introduces two children exploring the world around them. Produced by Krishna's Tribal Horse Entertainment, the series has a skilled technical crew, with Om Narayan on cinematography and Richard Kevin editing. Set to air on Disney+ Hotstar, this show is aimed at viewers who enjoy heartfelt family narratives.

The OTT release of Amaran starring Sivakarthikeyan has been delayed by a week as its strong box office performance continues, surpassing ₹200 crore in two weeks. Originally scheduled for Netflix after 28 days, Amaran will now be available mid-December. The film, based on Major Mukund Varadarajan's life, is directed by Rajkumar Periasamy. With audiences in Tamil Nadu, Kerala, and Karnataka filling theatres, this delay represents a rare shift to maximise earnings before its digital debut.

Cross, the new Prime Video crime-thriller series, debuts on November 14, 2024. Featuring Aldis Hodge as Alex Cross, this adaptation goes deeper into the detectives life than previous film versions. With 8 episodes in season 1, the show promises intense action and emotional depth as Cross faces a series of personal and professional challenges. The series marks a reimagined take on James Pattersons iconic character, with all episodes dropping on the same day for binge-watchers.

The supernatural horror fantasy The Watchers will stream on JioCinema in India beginning November 14, 2024. Directed by Ishana Night Shyamalan, the film stars Dakota Fanning as Mina, a young artist trapped in an eerie Irish forest with nocturnal beasts pursuing her and three strangers. The Watchers has gained attention for its gripping story, chilling atmosphere and for being Ishana’s directorial debut. The film is adapted from A.M. Shine's 2021 novel and has already captivated horror fans worldwide.

The exclusive fan event will take place in Seoul on November 24

The trailer of Pushpa 2 will release on November 17

From highly-anticipated blockbusters to critically acclaimed indie darlings, our comprehensive guide has got you covered

Directed by Nikhil Advani, Kal Ho Naa Ho features Shah Rukh Khan as Aman Mathur, Saif Ali Khan as Rohit Patel and Preity Zinta as Naina Catherine Kapur

The cast also included Rani Mukerji, Manoj Bajpayee, Amitabh Bachchan, Hema Malini and Boman Irani.

Consumers continue to benefit from carrier expansion and insurer oversight The Delaware Department of Insurance (DOI) has published its annual performance and productivity data, indicating continued success in serving consumers throughout the state. “In the face of inflation, climate change, the pandemic, and other challenges, our priority remains the same: the residents of our great […]

Another banner year for consumer protection and insurer competition In 2023, the Delaware Department of Insurance’s 101 team members served Delaware residents and seniors, attracted new companies, oversaw expansions of coverage and enhanced competition, and contributed substantial funds to the state. Today’s data release details how each team contributed to Delaware’s strong insurance environment. “Each […]

The Delaware Public Archives is pleased to announce the digital release of “The Abram H. Draper Collection.” This unique collection consists of 34 pieces of correspondence including letters and poetry from Sergeant Abram H. Draper to his wife Anna M. Wiley Draper during the American Civil War. The content ranges from detailed descriptions of Draper’s […]

Today, the Delaware Public Service Commission (PSC) released The Final Report assessing the conduct and results of Delmarva Power’s electric Standard Offer Service (SOS) reverse auction process for the 2021-2022 procurement year; the PSC was assisted by its technical consultant, Siemens PTI. Delmarva Power successfully secured all of the outstanding needs for full-requirement service for […]

Report addresses firearm, claims of new evidence The Department of Justice’s Division of Civil Rights & Public Trust (DCRPT) released Tuesday a supplemental report regarding the September 23, 2015 police shooting of Jeremy McDole. After the DOJ was presented in June with claims of new evidence and concerns about the validity of firearms evidence cited […]

Recommendations include housing, trauma training, expanding recovery resources in the criminal justice system The Delaware Drug Overdose Fatality Review Commission (DOFRC) has released its second annual report to Governor John Carney and the General Assembly. The 18-page report, released Tuesday, details five policy recommendations based on its review of a sample of 130 overdose fatalities […]

Attorney General Kathy Jennings released Tuesday a legislative agenda outlining ten policy priorities that she will advocate to the 151st General Assembly in 2021. “I know what we’re capable of in 2021, because I know what we accomplished together in the last two years,” said Attorney General Jennings. “We’ve made real progress, from historic criminal […]