AVR128DB28

CES 2020, LAS VEGAS – JANUARY 6, 2020 – Movie lovers, listen up. The latest addition to JBL’s popular Bar Series brings the audio experience of a movie theater to your living room. The JBL Bar 9.1 creates true wireless surround sound with crowd-pleasing...

Een gestroomlijnd model, krachtige bas en nog intenser geluid: JBL tilt home entertainment naar een hoger plan. Op IFA worden vandaag drie splinternieuwe producten gelanceerd: een compacte alles-in-één oplossing, een kamervullende surround-sound en een diepe bas Bar. Voor ieder wat wils in de nieuwe Soundbar serie.

Slimmer, punchier bass and more immersive sound; JBL has taken home entertainment to the next level. With three brand new products launching today at IFA including a compact, all-in-one solution, a deep bass beauty and a surround-sound room-filler, there is something for everyone in JBL’s new soundbar range.

Slimmer, punchier bass and more immersive sound; JBL has taken home entertainment to the next level. With three brand new products launching today at IFA including a compact, all-in-one solution, a deep bass beauty and a surround-sound room-filler, there is something for everyone in JBL’s new soundbar range.

This Pi Day, try calculating everyone’s favourite mathematical constant using balls and a cardboard tube, thanks to a mathematical trick involving the balls’ masses

The Good Place, a sitcom on Netflix about an afterlife with characters who represent me at my worst – and best – is over, but I can’t stop rewatching the show, says Chelsea Whyte

Title: Returning to Intimacy After Childbirth
Category: Health News
Created: 5/1/2019 12:00:00 AM
Last Editorial Review: 5/1/2019 12:00:00 AM

Many doctors are considering retiring, but fear they'll miss the patient interaction, mental stimulation, and income. There are ways to have it all.

Title: Bedbugs (Bed Bugs) vs. Lice
Category: Diseases and Conditions
Created: 2/18/2020 12:00:00 AM
Last Editorial Review: 2/18/2020 12:00:00 AM

ABSTRACT

Two-component signaling systems (TCSs) function to detect environmental cues and transduce this information into a change in transcription. In its simplest form, TCS-dependent regulation of transcription entails phosphoryl-transfer from a sensory histidine kinase to its cognate DNA-binding receiver protein. However, in certain cases, auxiliary proteins may modulate TCSs in response to secondary environmental cues. Caulobacter crescentus FixT is one such auxiliary regulator. FixT is composed of a single receiver domain and functions as a feedback inhibitor of the FixL-FixJ (FixLJ) TCS, which regulates the transcription of genes involved in adaptation to microaerobiosis. We sought to define the impact of fixT on Caulobacter cell physiology and to understand the molecular mechanism by which FixT represses FixLJ signaling. fixT deletion results in excess production of porphyrins and premature entry into stationary phase, demonstrating the importance of feedback inhibition of the FixLJ signaling system. Although FixT is a receiver domain, it does not affect dephosphorylation of the oxygen sensor kinase FixL or phosphoryl-transfer from FixL to its cognate receiver FixJ. Rather, FixT represses FixLJ signaling by inhibiting the FixL autophosphorylation reaction. We have further identified a 4-cysteine motif in Caulobacter FixT that binds an Fe-S cluster and protects the protein from degradation by the Lon protease. Our data support a model in which the oxidation of this Fe-S cluster promotes the degradation of FixT in vivo. This proteolytic mechanism facilitates clearance of the FixT feedback inhibitor from the cell under normoxia and resets the FixLJ system for a future microaerobic signaling event.

IMPORTANCE Two-component signal transduction systems (TCSs) are broadly conserved in the bacterial kingdom and generally contain two molecular components, a sensor histidine kinase and a receiver protein. Sensor histidine kinases alter their phosphorylation state in direct response to a physical or chemical cue, whereas receiver proteins "receive" the phosphoryl group from the kinase to regulate a change in cell physiology. We have discovered that a single-domain receiver protein, FixT, binds an Fe-S cluster and controls Caulobacter heme homeostasis though its function as a negative-feedback regulator of the oxygen sensor kinase FixL. We provide evidence that the Fe-S cluster protects FixT from Lon-dependent proteolysis in the cell and endows FixT with the ability to function as a second, autonomous oxygen/redox sensor in the FixL-FixJ signaling pathway. This study introduces a novel mechanism of regulated TCS feedback control by an Fe-S-binding receiver domain.

A growing number of small secreted peptides (SSPs) in plants are recognized as important regulatory molecules with roles in processes such as growth, development, reproduction, stress tolerance, and pathogen defense. Recent discoveries further implicate SSPs in regulating root nodule development, which is of particular significance for legumes. SSP-coding genes are frequently overlooked, because genome annotation pipelines generally ignore small open reading frames, which are those most likely to encode SSPs. Also, SSP-coding small open reading frames are often expressed at low levels or only under specific conditions, and thus are underrepresented in non-tissue-targeted or non-condition-optimized RNA-sequencing projects. We previously identified 4,439 SSP-encoding genes in the model legume Medicago truncatula. To support systematic characterization and annotation of these putative SSP-encoding genes, we developed the M. truncatula Small Secreted Peptide Database (MtSSPdb; https://mtsspdb.noble.org/). MtSSPdb currently hosts (1) a compendium of M. truncatula SSP candidates with putative function and family annotations; (2) a large-scale M. truncatula RNA-sequencing-based gene expression atlas integrated with various analytical tools, including differential expression, coexpression, and pathway enrichment analyses; (3) an online plant SSP prediction tool capable of analyzing protein sequences at the genome scale using the same protocol as for the identification of SSP genes; and (4) information about a library of synthetic peptides and root and nodule phenotyping data from synthetic peptide screens in planta. These datasets and analytical tools make MtSSPdb a unique and valuable resource for the plant research community. MtSSPdb also has the potential to become the most complete database of SSPs in plants.

Site-specific arrest of RNA polymerases (RNAPs) is fundamental to several technologies that assess RNA structure and function. Current in vitro transcription “roadblocking” approaches inhibit transcription elongation by blocking RNAP with a protein bound to the DNA template. One limitation of protein-mediated transcription roadblocking is that it requires inclusion of a protein factor extrinsic to the minimal in vitro transcription reaction. In this work, we developed a chemical approach for halting transcription by Escherichia coli RNAP. We first established a sequence-independent method for site-specific incorporation of chemical lesions into dsDNA templates by sequential PCR and translesion synthesis. We then show that interrupting the transcribed DNA strand with an internal desthiobiotin-triethylene glycol modification or 1,N6-etheno-2'-deoxyadenosine base efficiently and stably halts Escherichia coli RNAP transcription. By encoding an intrinsic stall site within the template DNA, our chemical transcription roadblocking approach enables display of nascent RNA molecules from RNAP in a minimal in vitro transcription reaction.

In the Central Western Carpathians (CWC), most published paleomagnetic results from Permo-Mesozoic rocks document extensive remagnetizations and come from thin-skinned thrust units that have undergone multistage deformation. We present results from lower Triassic redbeds from the autochthonous cover overlying the basement that carry a primary magnetization. Petromagnetic results indicate that the dominant ferromagnetic carrier is hematite, while magnetic susceptibility and its anisotropy are controlled by both ferromagnetic and paramagnetic minerals. Magnetic fabrics document weak deformation related to Late Cretaceous shortening. The directions of the high unblocking temperature remanence components pass both reversal and fold tests, attesting to their primary nature. Paleomagnetic inclinations are flatter than expected from reference datasets, suggesting small latitudinal separation between the CWC and stable Europe. Paleomagnetic declinations are mostly clustered within individual mountain massifs, implying their tectonic coherence. They show only minor differences between the massifs, indicating a lack of significant vertical-axis tectonic rotations within the studied central parts of the CWC. The paleomagnetic declinations are therefore representative of the whole of the CWC in terms of regional paleogeographic interpretations, and imply moderate counterclockwise rotations (c. 26°) of the region with respect to stable Europe since the Early Triassic.

Peripheral somatosensory input is modulated in the dorsal spinal cord by a network of excitatory and inhibitory interneurons. PTF1A is a transcription factor essential in dorsal neural tube progenitors for specification of these inhibitory neurons. Thus, mechanisms regulating Ptf1a expression are key for generating neuronal circuits underlying somatosensory behaviors. Mutations targeted to distinct cis-regulatory elements for Ptf1a in mice, tested the in vivo contribution of each element individually and in combination. Mutations in an autoregulatory enhancer resulted in reduced levels of PTF1A, and reduced numbers of specific dorsal spinal cord inhibitory neurons, particularly those expressing Pdyn and Gal. Although these mutants survive postnatally, at ~3–5 wk they elicit a severe scratching phenotype. Behaviorally, the mutants have increased sensitivity to itch, but acute sensitivity to other sensory stimuli such as mechanical or thermal pain is unaffected. We demonstrate a requirement for positive transcriptional autoregulatory feedback to attain the level of the neuronal specification factor PTF1A necessary for generating correctly balanced neuronal circuits.

MG53 is a member of the TRIM protein family that is predominantly expressed in striated muscles and participates in cell membrane repair. Controversy exists regarding MG53’s role in insulin signaling and manifestation of diabetes. We generated db/db mice with either whole-body ablation or sustained elevation of MG53 in the bloodstream in order to evaluate the physiological function of MG53 in diabetes. To quantify the amount of MG53 protein in circulation, we developed a monoclonal antibody against MG53 with high specificity. Western blot using this antibody revealed lower or no change of serum MG53 levels in db/db mice or patients with diabetes compared with control subjects. Neither whole-body ablation of MG53 nor sustained elevation of MG53 in circulation altered insulin signaling and glucose handling in db/db mice. Instead, mice with ablation of MG53 were more susceptible to streptozotocin-induced dysfunctional handling of glucose compared with the wild-type littermates. Alkaline-induced corneal injury demonstrated delayed healing in db/db mice, which was restored by topical administration of recombinant human (rh)MG53. Daily intravenous administration of rhMG53 in rats at concentrations up to 10 mg/kg did not produce adverse effects on glucose handling. These findings challenge the hypothetical function of MG53 as a causative factor for the development of diabetes. Our data suggest that rhMG53 is a potentially safe and effective biologic to treat diabetic oculopathy in rodents.

There is an ongoing need for safe and effective anti-bedbug compounds. Here, we tested the toxicity of three antimicrobial agents against bedbugs when administered orally. We reveal that doxycycline has direct insecticidal activity at 250 μg/ml (0.025%) that is particularly strong against immature bedbugs and appears to be independent of antimicrobial activity. Future studies to determine the mechanisms behind this property could be useful for the development of orally active insecticides or anti-bedbug therapeutics.

Sonos has been busy these past few months.

In this very special episode of the RPGCast, there’s a Christmas miracle! That’s right, we come up with a useful idea for the Wii U...

From realism in Final Fantasy games to the sordid history of Pokémon patches, we get all sorts of feedback this week. And then we play...

Some people answer some questions. Chris tries to give us a Just Cause for why he attached that cow to that helicopter. Alice gets Fruity...

Lots of mechs today. Watch out that they don’t break you. Were you a supporter of Obsidian’s last kickstarter? See when you get your reward...

One in six Taiwanese people suffered from foodborne illness annually during a four year period studied by researchers. From 2012 to 2015, almost 3.9 million foodborne illnesses and 50 deaths occurred annually in the country. Scientists said the study, published in the Journal of the Formosan Medical Association, provided the first national estimates on the... Continue Reading

After stories of people not being allowed to say goodbye to loved ones dying of coronavirus, hospitals around the world are moving to change the rules.

The different hominid species, possibly including the oldest-known Homo erectus, existed in the region's hills and caves

Keyboard aside, cheaper storage and 10th-generation Intel CPUs headline this refresh.

Follow our live coronavirus updates HERE Coronavirus: the symptoms

Follow our live coronavirus updates HERE Coronavirus: The symptoms

Virgin Media customers are still complaining about issues with their broadband despite the provider saying the issue has been fixed.

This is the heartbreaking moment a carer read a granddaughter's final goodbye to her grandmother, just two days before she died while suffering coronavirus symptoms.

London's Nightingale hospital will be put "on standby" within days as a result of the capital passing the peak of coronavirus cases.

Pai’s “baffling” report ignores broadband gaps and high prices, Democrats say.

Small ISPs want investigation as Frontier tries to block FCC funding for rivals.

Pandemic disrupts broadband progress as cities halt construction.

The stocks of DB Realty and Unitech rose after top executives of these companies were granted bail in the 2G scam case.

Linda Yaccarino, the hard-charging ad-sales chief of NBCUniversal. will soon be running into uncharted territory. In recent years, Yaccarino has railed against Nielsen and taken a public swipe at Facebook. She has urged advertisers to consider running fewer commercials on NBC and to  work to make the ones that remain more ambitious and interesting. On […]

'As if America hasn't been anti-gay and anti-woman from the outset, or killed and displaced millions of Muslims, including women and queers,' one critic retorted

Parsons stars in Ryan Murphy's new Netflix series, Hollywood

Holden is raising money for the NHS through the release of her first single

Most of us get high-speed internet via a wired connection. But not everyone has access, like in rural areas. 5G could broaden those broadband options.

The 50-year-old stars alongside Scarlett Johansson in the upcoming Marvel spin-off movie.

Raheem Sterling believes team-mate David Silva's will be "sorely missed" as Manchester City prepare for life without their Spanish superstar.

Premier League clubs have put all players based abroad on standby as a return to training draws near.

As vulnerable patients arrive at one hospital's psychiatric ward with just the clothes on their back, a support service is ensuring access to free, clean garments of their choice.

China's government is putting on a show of sending aid to countries suffering from COVID-19, but undermining efforts to control the source of further pandemics.