1 Estonian Kroon = 42.4191 CFA Franc BCEAO

1 Danish Krone = 87.9245 CFA Franc BCEAO

1 Fiji Dollar = 268.5261 CFA Franc BCEAO

1 New Zealand Dollar = 371.3479 CFA Franc BCEAO

1 Croatian Kuna = 87.1935 CFA Franc BCEAO

1 Peruvian Nuevo Sol = 177.9914 CFA Franc BCEAO

1 Dominican Peso = 10.9919 CFA Franc BCEAO

1 Papua New Guinean Kina = 176.3653 CFA Franc BCEAO

1 Brunei Dollar = 428.0882 CFA Franc BCEAO

Currently I tried this:

axlDBCreateShape(recPolyPlanes t "BOUNDARY/L02" netName sym1)

I get a atom error on car(sym1)

I can do this "static" using ETCH/L02 with out an issue, but I am trying to avoid doing an axlShapeChangeDynamicType().

Thanks,

Jerry

I have made a customized menu in PCB Editor which I now would like to fill with content.

First of all I would like to have commands to add (or delete) layers in the board. I have parameter files (.prm) that describes both the stackup and the artwork for 2, 4, 6 and 8 layers.

I guess I could record a script (macro) where I use the "Import Parameter file" dialogue but this will get windows flickering by etc. Can I do this with SKILL instead?

I realize that it is possible (somehow) to do a SKILL-script that completely builds up the stackup and artworks for boards with different number of layers but I then have to edit the SKILL everytime I need to change anything. My thinking is that it perhaps is easier just to call the prm-file, which is easy to modify from within Allegro without knowing anything about SKILL.

I'm also looking for a solution to remove some Subclasses, containing certain keywords with a SKILL script but since I'm completely new to SKILL I don't really know where to begin.

Any assistance would be much appreciated.

Hi, I am in the middle of a design and had no problem going back and forth between schematics and layout. Now I am getting the error message below. I am using Cadence 17.2.

ERROR: Layout database has probably been reverted to an earlier version than that, which was used in the latest flow or the schematic database was synchronized with another board.

The basecopy file generated by the last back-to-front flow not found.

ERROR: Layout database has probably been reverted to an earlier version than that, which was used in the latest flow or the schematic database was synchronized with another board.

The basecopy file generated by the last back-to-front flow not found.

Error: CMFBC-1: The schematic and the layout constraints were not synchronized as the changes done since the last sync up could not be reconciled. Syncing the current version of the schematic or layout databases with a previous version would result in this issue. The  constraint difference report is displayed.

Continuing with "changes-only" processing may result in incorrect constraint updates.

Thanks for your input

Claudia

ગુજરાતીઓ માટે આનંદના સમાચાર છે, જુનાગઢનો સંદિપ સાવલિયા KBCમાં જીત્યો મોટુ ઇનામ

Many Cisco devices such as Cisco RV340, Cisco RV340W, Cisco RV345, Cisco RV345P, Cisco RV260, Cisco RV260P, Cisco RV260W, Cisco 160, and Cisco 160W suffer from having hard-coded credentials, known GNU glibc, known BusyBox, and IoT Inspector identified vulnerabilities.

Clarion Energy’s Teresa Hansen, vice president of global content, for a webcast Wednesday  will be making some important announcements regarding this year’s event in New Orleans. Hansen also will offer key details on content, the exhibit floor and resources available to attendees.

Clarion Energy’s Teresa Hansen, vice president of global content, for a webcast Wednesday  will be making some important announcements regarding this year’s event in New Orleans. Hansen also will offer key details on content, the exhibit floor and resources available to attendees.

Make money first, save the planet second. Those are the reasons for most investors to get into green finance, according to HSBC Holdings Plc.

Canadian utility BC Hydro and the McLeod Lake Indian Band have forged an agreement that will give the aboriginal group "economic development opportunities and other benefits" related to the construction and operation of the Site C hydroelectric project.

Clarion Energy’s Teresa Hansen, vice president of global content, for a webcast Wednesday  will be making some important announcements regarding this year’s event in New Orleans. Hansen also will offer key details on content, the exhibit floor and resources available to attendees.

Indonesian Business & Charter Aviation Summit (IBCAS) 2020 is an annual conference and exhibition for Business and Charter Aviation organised by Avcon Group.

Clarion Energy’s Teresa Hansen, vice president of global content, for a webcast Wednesday  will be making some important announcements regarding this year’s event in New Orleans. Hansen also will offer key details on content, the exhibit floor and resources available to attendees.

This webcast gives 6 tips for keeping employees safe and mitigating security threats as your workforce goes remote.
Learn how to protect employees from malicious web content.

This webcast gives 6 tips for keeping employees safe and mitigating security threats as your workforce goes remote.
Learn how to protect employees from malicious web content.

This webcast gives 6 tips for keeping employees safe and mitigating security threats as your workforce goes remote.
Learn how to protect employees from malicious web content.

This webcast gives 6 tips for keeping employees safe and mitigating security threats as your workforce goes remote.
Learn how to protect employees from malicious web content.

This webcast gives 6 tips for keeping employees safe and mitigating security threats as your workforce goes remote.
Learn how to protect employees from malicious web content.

This webcast gives 6 tips for keeping employees safe and mitigating security threats as your workforce goes remote.
Learn how to protect employees from malicious web content.

This webcast gives 6 tips for keeping employees safe and mitigating security threats as your workforce goes remote.
Learn how to protect employees from malicious web content.

As the government pronounced illegal on the practice of companies relying on subcontract workers for a long time such as the cases in Paris Baguette and Mando-Hella, the whole industry in shock. This is not just restricted to manufacturers but is spread to services and the food franchise industry as well. The corporate sector expected on September 24 that companies like Tous Les Jours, Samsung Electronics service centers, and LG U+ will likely face the same problem any time soon after the Min...

South Africa vaccinates health workers in response to the pandemic.

This video defines the various types of Subcontractors and how they are assessed and utilized in the performance of ADB projects.

ATA663431/54 LIN SBC including LIN Transceiver, FranVoltage Regulator, Window Watchdog and High-Side Switch

In order to expedite the completion of eight stalled residential projects in Noida and Greater Noida, the National Buildings Construction Corporation Limited (NBCC) would soon issue a tender. The NBCC has plans to float a tender for completing the eight stalled housing projects of Amrapali, which includes over 23,000 residential units in Noida and Greater Noida with a total investment of over Rs 5,000 crore. The decision to issue the tender was made after the directive issued by the Supreme Court (SC) to take over the stalled projects in the region. Last year, the apex court had given the responsibility to State-owned NBCC to complete the delayed projects in NCR at the […]

In 2012, mathematician Shinichi Mochizuki produced a proof claiming to solve the long-standing ABC conjecture, but no one understood it. Most mathematicians still don't, but it will now be published in a journal

Megafloods, broken backstops and retreating ice sheets combine in a geological epic: the dramatic story of Britain's protracted original exit from Europe

A long-standing hypothesis suggests the BCG vaccine also serves to generally enhance the immune system, meaning it could protect against covid-19, and trials are under way to find out

The BCG tuberculosis vaccine boosts the production of immune cells and this may explain how it protects newborns from dying of sepsis

ABSTRACT

The wall teichoic acid (WTA) is a major cell wall component of Gram-positive bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), a common cause of fatal clinical infections in humans. Thus, the indispensable ABC transporter TarGH, which flips WTA from cytoplasm to extracellular space, becomes a promising target of anti-MRSA drugs. Here, we report the 3.9-Å cryo-electron microscopy (cryo-EM) structure of a 50% sequence-identical homolog of TarGH from Alicyclobacillus herbarius at an ATP-free and inward-facing conformation. Structural analysis combined with activity assays enables us to clearly decode the binding site and inhibitory mechanism of the anti-MRSA inhibitor Targocil, which targets TarGH. Moreover, we propose a "crankshaft conrod" mechanism utilized by TarGH, which can be applied to similar ABC transporters that translocate a rather big substrate through relatively subtle conformational changes. These findings provide a structural basis for the rational design and optimization of antibiotics against MRSA.

IMPORTANCE The wall teichoic acid (WTA) is a major component of cell wall and a pathogenic factor in methicillin-resistant Staphylococcus aureus (MRSA). The ABC transporter TarGH is indispensable for flipping WTA precursor from cytoplasm to the extracellular space, thus making it a promising drug target for anti-MRSA agents. The 3.9-Å cryo-EM structure of a TarGH homolog helps us to decode the binding site and inhibitory mechanism of a recently reported inhibitor, Targocil, and provides a structural platform for rational design and optimization of potential antibiotics. Moreover, we propose a "crankshaft conrod" mechanism to explain how a big substrate is translocated through subtle conformational changes of type II exporters. These findings advance our understanding of anti-MRSA drug design and ABC transporters.

ABSTRACT

This research analyzed six Aspergillus fumigatus genes encoding putative efflux proteins for their roles as transporters. The A. fumigatus genes abcA, abcC, abcF, abcG, abcH, and abcI were cloned into plasmids and overexpressed in a Saccharomyces cerevisiae strain in which the highly active endogenous ABC transporter gene PDR5 was deleted. The activity of each transporter was measured by efflux of rhodamine 6G and accumulation of alanine β-naphthylamide. The transporters AbcA, AbcC, and AbcF had the strongest efflux activities of these compounds. All of the strains with plasmid-expressed transporters had more efflux activity than did the PDR5-deleted background strain. We performed broth microdilution drug susceptibility testing and agar spot assays using an array of compounds and antifungal drugs to determine the transporter specificity and drug susceptibility of the strains. The transporters AbcC and AbcF showed the broadest range of substrate specificity, while AbcG and AbcH had the narrowest range of substrates. Strains expressing the AbcA, AbcC, AbcF, or AbcI transporter were more resistant to fluconazole than was the PDR5-deleted background strain. Strains expressing AbcC and AbcF were additionally more resistant to clotrimazole, itraconazole, ketoconazole, and posaconazole than was the background strain. Finally, we analyzed the expression levels of the genes by reverse transcription-quantitative PCR (RT-qPCR) in triazole-susceptible and -resistant A. fumigatus clinical isolates. All of these transporters are expressed at a measurable level, and transporter expression varied significantly between strains, demonstrating the high degree of phenotypic variation, plasticity, and divergence of which this species is capable.

IMPORTANCE One mechanism behind drug resistance is altered export out of the cell. This work is a multifaceted analysis of membrane efflux transporters in the human fungal pathogen A. fumigatus. Bioinformatics evidence infers that there is a relatively large number of genes in A. fumigatus that encode ABC efflux transporters. However, very few of these transporters have been directly characterized and analyzed for their potential role in drug resistance.

Our objective was to determine if these undercharacterized proteins function as efflux transporters and then to better define whether their efflux substrates include antifungal drugs used to treat fungal infections. We chose six A. fumigatus potential plasma membrane ABC transporter genes for analysis and found that all six genes produced functional transporter proteins. We used two fungal systems to look for correlations between transporter function and drug resistance. These transporters have the potential to produce drug-resistant phenotypes in A. fumigatus. Continued characterization of these and other transporters may assist in the development of efflux inhibitor drugs.

Addressing social drivers of health in medical education—through community engagement experiences—is essential for health equity and the development of future physicians. While this was written before the COVID-19 pandemic, these practices will gain even more importance as we come together to better understand its health and community implications in North Carolina and the United States.

The Bcl-2 (B cell lymphoma 2)-related protein Nr-13 plays a major role in the regulation of cell death in developing avian B cells. With over 65% sequence similarity to the chicken Nr-13, herpesvirus of turkeys (HVT) vNr-13, encoded by the HVT079 and HVT096 genes, is the first known alphaherpesvirus-encoded Bcl-2 homolog. HVT-infected cells were reported to be relatively more resistant to serum starvation, suggested that vNr-13 could be involved in protecting the cells. Here, we describe CRISPR/Cas9-based editing of exon 1 of the HVT079 and HVT096 genes from the HVT genome to generate the mutant HVT-vNr-13 to gain insights into its functional roles. Overall, wild-type HVT and HVT-vNr-13 showed similar growth kinetics; however, at early time points, HVT-vNr-13 showed 1.3- to 1.7-fold-lower growth of cell-associated virus and 3- to 6.2-fold-lower growth of cell-free virus. In transfected cells, HVT vNr-13 showed a mainly diffuse cytoplasmic distribution with faint nuclear staining. Further, vNr-13 localized to the mitochondria and endoplasmic reticulum (ER) and disrupted mitochondrial network morphology in the transfected cells. In the wild-type HVT-infected cells, vNr-13 expression appeared to be directly involved in the disruption of the mitochondrial network, as the mitochondrial network morphology was substantially restored in the HVT-vNr-13-infected cells. IncuCyte S3 real-time apoptosis monitoring demonstrated that vNr-13 is unequivocally involved in the apoptosis inhibition, and it is associated with an increase of PFU, especially under serum-free conditions in the later stages of the viral replication cycle. Furthermore, HVT blocks apoptosis in infected cells but activates apoptosis in noninfected bystander cells.

IMPORTANCE B cell lymphoma 2 (Bcl-2) family proteins play important roles in regulating apoptosis during homeostasis, tissue development, and infectious diseases. Several viruses encode homologs of cellular Bcl-2-proteins (vBcl-2) to inhibit apoptosis, which enable them to replicate and persist in the infected cells and to evade/modulate the immune response of the host. Herpesvirus of turkeys (HVT) is a nonpathogenic alphaherpesvirus of turkeys and chickens that is widely used as a live vaccine against Marek’s disease and as recombinant vaccine viral vectors for protecting against multiple avian diseases. Identical copies of the HVT genes HVT079 and HVT096 encode the Bcl-2 homolog vNr-13. While previous studies have identified the potential ability of vNr-13 in inhibiting apoptosis induced by serum deprivation, there have been no detailed investigations on the functions of vNr-13. Using CRISPR/Cas9-based ablation of the vNr-13 gene, we demonstrated the roles of HVT vNr-13 in early stages of the viral replication cycle, mitochondrial morphology disruption, and apoptosis inhibition in later stages of viral replication.

Triple-negative breast cancer (TNBC) accounts for 10 to 20% of breast cancer, with chemotherapy as its mainstay of treatment due to lack of well-defined targets, and recent genomic sequencing studies have revealed a paucity of TNBC-specific mutations. Recurrent gene fusions comprise a class of viable genetic targets in solid tumors;...

SUMOylation is a posttranslational modification (PTM) at a lysine residue and is crucial for the proper functions of many proteins, particularly of transcription factors, in various biological processes. Zinc finger homeobox 3 (ZFHX3), also known as AT motif-binding factor 1 (ATBF1), is a large transcription factor that is active in multiple pathological processes, including atrial fibrillation and carcinogenesis, and in circadian regulation and development. We have previously demonstrated that ZFHX3 is SUMOylated at three or more lysine residues. Here, we investigated which enzymes regulate ZFHX3 SUMOylation and whether SUMOylation modulates ZFHX3 stability and function. We found that SUMO1, SUMO2, and SUMO3 each are conjugated to ZFHX3. Multiple lysine residues in ZFHX3 were SUMOylated, but Lys-2806 was the major SUMOylation site, and we also found that it is highly conserved among ZFHX3 orthologs from different animal species. Using molecular analyses, we identified the enzymes that mediate ZFHX3 SUMOylation; these included SUMO1-activating enzyme subunit 1 (SAE1), an E1-activating enzyme; SUMO-conjugating enzyme UBC9 (UBC9), an E2-conjugating enzyme; and protein inhibitor of activated STAT2 (PIAS2), an E3 ligase. Multiple analyses established that both SUMO-specific peptidase 1 (SENP1) and SENP2 deSUMOylate ZFHX3. SUMOylation at Lys-2806 enhanced ZFHX3 stability by interfering with its ubiquitination and proteasomal degradation. Functionally, Lys-2806 SUMOylation enabled ZFHX3-mediated cell proliferation and xenograft tumor growth of the MDA-MB-231 breast cancer cell line. These findings reveal the enzymes involved in, and the functional consequences of, ZFHX3 SUMOylation, insights that may help shed light on ZFHX3's roles in various cellular and pathophysiological processes.

Formate oxidation to carbon dioxide is a key reaction in one-carbon compound metabolism, and its reverse reaction represents the first step in carbon assimilation in the acetogenic and methanogenic branches of many anaerobic organisms. The molybdenum-containing dehydrogenase FdsABG is a soluble NAD+-dependent formate dehydrogenase and a member of the NADH dehydrogenase superfamily. Here, we present the first structure of the FdsBG subcomplex of the cytosolic FdsABG formate dehydrogenase from the hydrogen-oxidizing bacterium Cupriavidus necator H16 both with and without bound NADH. The structures revealed that the two iron-sulfur clusters, Fe4S4 in FdsB and Fe2S2 in FdsG, are closer to the FMN than they are in other NADH dehydrogenases. Rapid kinetic studies and EPR measurements of rapid freeze-quenched samples of the NADH reduction of FdsBG identified a neutral flavin semiquinone, FMNH•, not previously observed to participate in NADH-mediated reduction of the FdsABG holoenzyme. We found that this semiquinone forms through the transfer of one electron from the fully reduced FMNH−, initially formed via NADH-mediated reduction, to the Fe2S2 cluster. This Fe2S2 cluster is not part of the on-path chain of iron-sulfur clusters connecting the FMN of FdsB with the active-site molybdenum center of FdsA. According to the NADH-bound structure, the nicotinamide ring stacks onto the re-face of the FMN. However, NADH binding significantly reduced the electron density for the isoalloxazine ring of FMN and induced a conformational change in residues of the FMN-binding pocket that display peptide-bond flipping upon NAD+ binding in proper NADH dehydrogenases.