Chris bought recording gear off an Instagram ad, our thoughts on WebC, CodePen upgrades Yarn, thoughts on the commercial value of open source, Automattic releases an app to install WordPress locally, IBM buys Hashicorp, income tax software, and a hack for getting Safari to respect background colors used in a pseudo selector.

A groundwater expert uses a webcam to find out the yield from borewells and identify problems. By

A quick primer on how to manage all that rich organic waste your home generates

Promoter tactics to scuttle process, the main problem

The highest price fetched during the e-auction for a unit (at the World Trade Centre) was ₹62,261 per sq ft

The project marks an essential phase in KBC Global’s international ventures, promising residential complexes, low-cost housing and commercial spaces within Liberia’s SEZ

He demanded Telangana government to immediately release reimbursement and scholarship dues amount to about ₹4,000 crore

There is a high possibility that the five-match Test series against Australia could be the final one for at least two of the four seniors among Rohit Sharma, Virat Kohli, Ravindra Jadeja and Ravichandran Ashwin.

The player registration officially closed and a total of 1,574 cricketers (1,165 Indian and 409 overseas) have been signed up for the auction

The Hybrid Model was followed during 2023 Asia Cup where Pakistan played its matches at home but India played its matches in Sri Lanka.

According to a source, the meeting was not a knee-jerk reaction to India’s series whitewash (0-3) at home by New Zealand

Just out of college and looking for a job? Some tips on professional etiquette.

BJP leaders from various backward class communities are entrusted with the task of micro-managing voters, since JD (S) lacks many such leaders in its ranks

BCCI decision comes despite Pakistan Cricket Board offering special security arrangements, choice of venue; India favours having its matches outside Pakistan under hybrid model

Lineker, who is the BBC’s highest-paid employee, was briefly taken off air after comparing the language used to launch a British government asylum policy to the rhetoric of Nazi-era Germany on social media.

Last month, Supreme Court had set aside a judgement of the National Company Law Appellate Tribunal (NCLAT) allowing a ₹158 crore settlement between ed-tech firm Byju’s with the BCCI.

NCC says charges trumped up based on unsustainable malicious aspersions

How do we tackle pests without synthetic pesticides? How can we get a huge haul of vegetables without resorting to chemical fertilisers? A workshop series teaches us organic gardening step-by-step

Plants dying mysteriously in your kitchen garden? It could be root disease, says Hema Vijay

Learn the ABCs of RSS Feeds

The appellate tribunal also dismissed the allegation of round-tripping levelled by Byju's U.S.-based lenders, saying they failed to provide any evidence for the same

Addressing the Assembly on Friday, the CM said that the Government would provide land if the BCCI agreed to build a stadium with international standards

Why has digital streaming rights garnered higher numbers in the auction? How has the league’s value improved?

BCCI has intimated the International Cricket Council about India's inability to travel to Pakistan for the Champions Trophy next year.

Company: P & I Management Consultants
Experience: 0 to 2
location: India, Ludhiana
Ref: 24603638
Summary: Job Description: Job Description Female graduate with experience of 0-2 years in office coordination, computer work, Customer Service, Receiving and making phone calls, follow up service calls. Confident Girls with....

Thanks to the unseasonal rain around Diwali, the extended monsoon and growing debt of farmers, non-performing assets (NPAs) among crop loans in Gujarat rose marginally by 3.2% in the third quarter of 2019-20.

1. Barack Obama labels Donald Trump's coronavirus response a 'chaotic disaster'  ABC News
2. Barack Obama labels Donald Trump’s coronavirus response ‘absolute chaotic disaster’  NEWS.com.au
3. Obama lashes out at Trump in call with supporters  Sydney Morning Herald
4. Coronavirus update: Barack Obama calls Donald Trump's coronavirus response a 'chaotic disaster'  ABC News
5. 'Chaotic disaster': Barack Obama unloads on Donald Trump's response to coronavirus  SBS News
6. View Full coverage on Google News

1. WA coronavirus restriction easing not enough for pubs, beauticians, tourism industry  ABC News
2. Coronavirus crisis: Weekends in Esperance back on the cards  The West Australian
3. WA's decision to keep its mines open amid coronavirus may have saved Australia's economy | ABC News  ABC News (Australia)
4. View Full coverage on Google News

1. Jackie Trad resigns as Queensland's Deputy Premier and Treasurer, Steven Miles and Cameron Dick to take on portfolios  ABC News
2. Poster girl’s fall a shock for the left of politics  The Australian
3. Corruption investigation into Queensland Deputy Premier Jackie Trad has potential to unravel Labor Government's five-year reign  ABC News
4. Editorial: Premier, Queensland needs a new treasurer now  Courier Mail
5. Analysis: Latest investigation not a good look for Jackie Trad  Courier Mail
6. View Full coverage on Google News

Bacterial biofilms are cellular communities that produce an adherent matrix. Exopolysaccharides are key structural components of this matrix and are required for the assembly and architecture of biofilms produced by a wide variety of microorganisms. The human bacterial pathogens Escherichia coli and Salmonella enterica produce a biofilm matrix composed primarily of the exopolysaccharide phosphoethanolamine (pEtN) cellulose. Once thought to be composed of only underivatized cellulose, the pEtN modification present in these matrices has been implicated in the overall architecture and integrity of the biofilm. However, an understanding of the mechanism underlying pEtN derivatization of the cellulose exopolysaccharide remains elusive. The bacterial cellulose synthase subunit G (BcsG) is a predicted inner membrane–localized metalloenzyme that has been proposed to catalyze the transfer of the pEtN group from membrane phospholipids to cellulose. Here we present evidence that the C-terminal domain of BcsG from E. coli (EcBcsGΔN) functions as a phosphoethanolamine transferase in vitro with substrate preference for cellulosic materials. Structural characterization of EcBcsGΔN revealed that it belongs to the alkaline phosphatase superfamily, contains a Zn2+ ion at its active center, and is structurally similar to characterized enzymes that confer colistin resistance in Gram-negative bacteria. Informed by our structural studies, we present a functional complementation experiment in E. coli AR3110, indicating that the activity of the BcsG C-terminal domain is essential for integrity of the pellicular biofilm. Furthermore, our results established a similar but distinct active-site architecture and catalytic mechanism shared between BcsG and the colistin resistance enzymes.

Multidrug resistance (MDR) in cancer arises from cross-resistance to structurally- and functionally-divergent chemotherapeutic drugs. In particular, MDR is characterized by increased expression and activity of ATP-binding cassette (ABC) superfamily transporters. Sphingolipids are substrates of ABC proteins in cell signaling, membrane biosynthesis, and inflammation, for example, and their products can favor cancer progression. Glucosylceramide (GlcCer) is a ubiquitous glycosphingolipid (GSL) generated by glucosylceramide synthase, a key regulatory enzyme encoded by the UDP-glucose ceramide glucosyltransferase (UGCG) gene. Stressed cells increase de novo biosynthesis of ceramides, which return to sub-toxic levels after UGCG mediates incorporation into GlcCer. Given that cancer cells seem to mobilize UGCG and have increased GSL content for ceramide clearance, which ultimately contributes to chemotherapy failure, here we investigated how inhibition of GSL biosynthesis affects the MDR phenotype of chronic myeloid leukemias. We found that MDR is associated with higher UGCG expression and with a complex GSL profile. UGCG inhibition with the ceramide analog d-threo-1-(3,4,-ethylenedioxy)phenyl-2-palmitoylamino-3-pyrrolidino-1-propanol (EtDO-P4) greatly reduced GSL and monosialotetrahexosylganglioside levels, and co-treatment with standard chemotherapeutics sensitized cells to mitochondrial membrane potential loss and apoptosis. ABC subfamily B member 1 (ABCB1) expression was reduced, and ABCC-mediated efflux activity was modulated by competition with nonglycosylated ceramides. Consistently, inhibition of ABCC-mediated transport reduced the efflux of exogenous C6-ceramide. Overall, UGCG inhibition impaired the malignant glycophenotype of MDR leukemias, which typically overcomes drug resistance through distinct mechanisms. This work sheds light on the involvement of GSL in chemotherapy failure, and its findings suggest that targeted GSL modulation could help manage MDR leukemias.

A woman in her 90s who lived in a long-term care home is the area's latest COVID-19-related death, officials said Saturday.

IT could have been a warm welcome home for a conquering comedy hero when Billy Connolly appeared before an audience of Scottish school children at the BBC in Glasgow.

Join us on June 12 as we take an in-depth look at video marketing strategy

Join us on June 12 as we take an in-depth look at video marketing strategy