Macrotech Developers acquires Bain Capital’s stake in three joint digital infrastructure platform entities for Rs 307 crore

The structure of the protonated eight-membered cluster of serines formed during electrospray ionization has long baffled mass spectrometrists

Crystalline needles of bone mineral form helical patterns around collagen fibrils

Company expects to slim its home country head count by 600

Synthetic construct could someday replace damaged counterparts in disease

Textured catalyst prevents gas bubbles from clinging to surfaces

Follow-up studies could lead to inhibitors that block uptake, thus stopping body odor production

The company has implemented a new leadership structure, dividing operations between two executive directors

WASHINGTON (April 27, 2020) — Today, the U.S.

WASHINGTON (April 28, 2020) — Today, the U.S.

Lethal infections by strains of the highly-pathogenic avian influenza virus (HPAIV) H5N1 pose serious threats to both the poultry industry and public health worldwide. A lack of confirmed HPAIV epitopes recognized by cytotoxic T lymphocytes (CTLs) has hindered the utilization of CD8+ T-cell–mediated immunity and has precluded the development of effectively diversified epitope-based vaccination approaches. In particular, an HPAIV H5N1 CTL-recognized epitope based on the peptide MHC-I–β2m (pMHC-I) complex has not yet been designed. Here, screening a collection of selected peptides of several HPAIV strains against a specific pathogen-free pMHC-I (pBF2*1501), we identified a highly-conserved HPAIV H5N1 CTL epitope, named HPAIV–PA123–130. We determined the structure of the BF2*1501–PA123–130 complex at 2.1 Å resolution to elucidate the molecular mechanisms of a preferential presentation of the highly-conserved PA123–130 epitope in the chicken B15 lineage. Conformational characteristics of the PA123–130 epitope with a protruding Tyr-7 residue indicated that this epitope has great potential to be recognized by specific TCRs. Moreover, significantly increased numbers of CD8+ T cells specific for the HPAIV–PA123–130 epitope in peptide-immunized chickens indicated that a repertoire of CD8+ T cells can specifically respond to this epitope. We anticipate that the identification and structural characterization of the PA123–130 epitope reported here could enable further studies of CTL immunity against HPAIV H5N1. Such studies may aid in the development of vaccine development strategies using well-conserved internal viral antigens in chickens.

The C-type lectin receptors (CLRs) form a family of pattern recognition receptors that recognize numerous pathogens, such as bacteria and fungi, and trigger innate immune responses. The extracellular carbohydrate-recognition domain (CRD) of CLRs forms a globular structure that can coordinate a Ca2+ ion, allowing receptor interactions with sugar-containing ligands. Although well-conserved, the CRD fold can also display differences that directly affect the specificity of the receptors for their ligands. Here, we report crystal structures at 1.8–2.3 Å resolutions of the CRD of murine dendritic cell-immunoactivating receptor (DCAR, or Clec4b1), the CLR that binds phosphoglycolipids such as acylated phosphatidyl-myo-inositol mannosides (AcPIMs) of mycobacteria. Using mutagenesis analysis, we identified critical residues, Ala136 and Gln198, on the surface surrounding the ligand-binding site of DCAR, as well as an atypical Ca2+-binding motif (Glu-Pro-Ser/EPS168–170). By chemically synthesizing a water-soluble ligand analog, inositol-monophosphate dimannose (IPM2), we confirmed the direct interaction of DCAR with the polar moiety of AcPIMs by biolayer interferometry and co-crystallization approaches. We also observed a hydrophobic groove extending from the ligand-binding site that is in a suitable position to interact with the lipid portion of whole AcPIMs. These results suggest that the hydroxyl group-binding ability and hydrophobic groove of DCAR mediate its specific binding to pathogen-derived phosphoglycolipids such as mycobacterial AcPIMs.

Inter-α-inhibitor is a proteoglycan essential for mammalian reproduction and also plays a less well-characterized role in inflammation. It comprises two homologous “heavy chains” (HC1 and HC2) covalently attached to chondroitin sulfate on the bikunin core protein. Before ovulation, HCs are transferred onto the polysaccharide hyaluronan (HA) to form covalent HC·HA complexes, thereby stabilizing an extracellular matrix around the oocyte required for fertilization. Additionally, such complexes form during inflammatory processes and mediate leukocyte adhesion in the synovial fluids of arthritis patients and protect against sepsis. Here using X-ray crystallography, we show that human HC1 has a structure similar to integrin β-chains, with a von Willebrand factor A domain containing a functional metal ion-dependent adhesion site (MIDAS) and an associated hybrid domain. A comparison of the WT protein and a variant with an impaired MIDAS (but otherwise structurally identical) by small-angle X-ray scattering and analytical ultracentrifugation revealed that HC1 self-associates in a cation-dependent manner, providing a mechanism for HC·HA cross-linking and matrix stabilization. Surprisingly, unlike integrins, HC1 interacted with RGD-containing ligands, such as fibronectin, vitronectin, and the latency-associated peptides of transforming growth factor β, in a MIDAS/cation-independent manner. However, HC1 utilizes its MIDAS motif to bind to and inhibit the cleavage of complement C3, and small-angle X-ray scattering–based modeling indicates that this occurs through the inhibition of the alternative pathway C3 convertase. These findings provide detailed structural and functional insights into HC1 as a regulator of innate immunity and further elucidate the role of HC·HA complexes in inflammation and ovulation.

Fucosylation of the innermost GlcNAc of N-glycans by fucosyltransferase 8 (FUT8) is an important step in the maturation of complex and hybrid N-glycans. This simple modification can dramatically affect the activities and half-lives of glycoproteins, effects that are relevant to understanding the invasiveness of some cancers, development of mAb therapeutics, and the etiology of a congenital glycosylation disorder. The acceptor substrate preferences of FUT8 are well-characterized and provide a framework for understanding N-glycan maturation in the Golgi; however, the structural basis of these substrate preferences and the mechanism through which catalysis is achieved remain unknown. Here we describe several structures of mouse and human FUT8 in the apo state and in complex with GDP, a mimic of the donor substrate, and with a glycopeptide acceptor substrate at 1.80–2.50 Å resolution. These structures provide insights into a unique conformational change associated with donor substrate binding, common strategies employed by fucosyltransferases to coordinate GDP, features that define acceptor substrate preferences, and a likely mechanism for enzyme catalysis. Together with molecular dynamics simulations, the structures also revealed how FUT8 dimerization plays an important role in defining the acceptor substrate-binding site. Collectively, this information significantly builds on our understanding of the core fucosylation process.

It’s not just your waistline that suffers as you put on weight. Researchers are beginning to find puzzling new links between obesity, memory loss and dementia

Lucy Cheke and her colleagues at the University of Cambridge recently invited a few participants into her lab for a kind of ‘treasure hunt’.

The participants navigated a virtual environment on a computer screen, dropping off various objects along their way. They then answered a series of questions to test their memory of the task, such as where they had hidden a particular object.

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Even agri-exports as a share of GDP is fairly lower for India relative to the rest of the world despite being one of the largest producers of agri-commodities globally.

May 10, 2020 Nasser Al Nasser: The Kingdom's infrastructure investments helped it contain the repercussions of the Corona crisis Eng. Nasser Sulaiman Al Nasser, stc Group CEO, said that he is proud of the Kingdom's infrastructure that helped it contain the repercussions of the Corona crisis that affected economy all over the world. Al Nasser said that investing in the digital infrastructure a long time ago has enabled the telecom sector undertake its central role in this crisis. Stc Group CEO said: 'I am proud of our country's achievements in facing these circumstances we are living. This...

[Daily News] Young Africans kit and merchandise suppliers GSM said restructuring the club's management system will be the first task towards the transformation processes.

The OECD, together with the African Development Bank and International Finance Corporation, will assist the Tunisian authorities in implementing a new law on public-private partnerships(PPPs)and a new investment code in order to increase transparency, openness and predictability for investors.

This seminar focused on overcoming challenges to private sector participation in infrastructure in Southern Africa.

This conference addressed key questions on investment in infrastructure and transport in Latin America and the Caribbean. It brought together policymakers, international experts and other interested stakeholders to discuss concrete policy recommendations to maximise the opportunities and address the challenges in the region.

This seminar investigated how changes made in key policy areas at national, regional and international levels can help generate more and better private investment in Africa’s infrastructure.

Adequate infrastructure is necessary for sustainable economic and social development. However investment in infrastructure in most developing and emerging economies needs to be substantially increased. This paper draws on 22 OECD Investment Policy Reviews undertaken in such economies and identifies policy options to enhance the enabling environment for infrastructure investment.

Addis Ababa - Part of the 3rd International Conference on Financing for Development, this event explored strategies to leverage Africa’s pension funds and other sources of private financing to develop Africa’s infrastructure. Ways to improve the investment climate in Africa using the recently updated Policy Framework for Investment were also be addressed.

When economic shocks occur, economic agents are expected to react to reduce the negative impact or amplify the positive effects. The ability of a country to contain economic losses can be defined as the resilience to economic shocks. Using the OECD Inter-Country Input-Output tables, this paper investigates the relationship between changes in final demand and production structures for 61 economies.

A new OECD report, the 2018 Business and Finance Outlook, highlights a number of major risks having the potential to disrupt global economic growth. It notes that the gradual normalisation of monetary policy in an environment of growing debt will be a major test of whether the Basel III regulatory reforms have achieved their goal of ensuring safety and soundness in the financial system.

A key issue for policy makers and regulators is market structures that will best deliver efficient and inclusive mobile communication services. This report addresses recent experience in selected countries that have changed or held constant the number of facilities-based operators; and initial experience and key questions that have arisen with wireless network sharing.

This seminar took place in Palembang, Indonesia, with discussions focusing on institutional investors and long-term financing and policy measures and initiatives to address constraints to infrastructure investment identified by APEC under the Indonesian presidency.

This G20 report looks at the evolution of private institutional investment in infrastructure and examines how the market has developed; analyses various investment vehicles with a snapshot of the growth experienced in the market; categorises methods used for investing in infrastructure; and, highlights the challenges and barriers to investment.

Addis Ababa - Part of the 3rd International Conference on Financing for Development, this event explored strategies to leverage Africa’s pension funds and other sources of private financing to develop Africa’s infrastructure. Ways to improve the investment climate in Africa using the recently updated Policy Framework for Investment were also be addressed.

This taxonomy maps out the investment options available to private investors and the instruments and incentives available to attract private sector investment in infrastructure.