U4SSC - City Snapshot - Herøy, Norway

U4SSC - City Snapshot - Hustadvika, Norway

U4SSC - City Snapshot - Ørsta, Norway

U4SSC - City Snapshot - Rauma, Norway

U4SSC - City Snapshot - Sande, Norway

U4SSC - City Snapshot - Smøla, Norway

U4SSC - City Snapshot - Stranda, Norway

U4SSC - City Snapshot - Sunndal, Norway

U4SSC - City Snapshot - Surnadal, Norway

U4SSC - City Snapshot - Sykkylven, Norway

U4SSC - City Snapshot - Tingvoll, Norway

U4SSC - City Snapshot - Ulstein, Norway

U4SSC - City Snapshot - Vanylven, Norway

U4SSC - City Snapshot - Vestnes, Norway

U4SSC - City Snapshot - Volda, Norway

U4SSC - City Snapshot - Hareid, Norway

U4SSC - City Snapshot - More og Romsdal, Norway

U4SSC - City Snapshot - Larvik, Norway

U4SSC - Snapshot - Mashhad, Iran

U4SSC - City Snapshot - Daegu, Korea

U4SSC - City Snapshot - Narvik, Norway

U4SSC - City Snapshot - TROMSØ, NORWAY

U4SSC - Snapshot - Geneva, Switzerland

U4SSC - City Snapshot - Kyebi, Ghana

U4SSC - City Snapshot - Anyang, Korea (Republic of)

Roofing Services Solutions, a Dunes Point Capital portfolio company, has acquired Earl W. Johnston Roofing, expanding its Florida presence to 10 locations and 200 employees.

WikiLeaks: 2009-12-15: 09STOCKHOLM779: Snapshot of Muslim Communities in Sweden

Look back at Volume 2024 through photos from our photographers Volume Music Festival's return after five years was nothing short of epic…

Nathan MacKinnon had his most productive night of the season to pull the Colorado Avalanche out of a funk, but the club's extraordinary injury crisis may have somehow reached a new level.

Perception is everything.

Highly engineered phytases, which sequentially hydrolyze the hexakisphosphate ester of inositol known as phytic acid, are routinely added to the feeds of monogastric animals to improve phosphate bioavailability. New phytases are sought as starting points to further optimize the rate and extent of dephosphorylation of phytate in the animal digestive tract. Multiple inositol polyphosphate phosphatases (MINPPs) are clade 2 histidine phosphatases (HP2P) able to carry out the stepwise hydrolysis of phytate. MINPPs are not restricted by a strong positional specificity making them attractive targets for development as feed enzymes. Here, we describe the characterization of a MINPP from the Gram-positive bacterium Bifidobacterium longum (BlMINPP). BlMINPP has a typical HP2P-fold but, unusually, possesses a large α-domain polypeptide insertion relative to other MINPPs. This insertion, termed the U-loop, spans the active site and contributes to substrate specificity pockets underpopulated in other HP2Ps. Mutagenesis of U-loop residues reveals its contribution to enzyme kinetics and thermostability. Moreover, four crystal structures of the protein along the catalytic cycle capture, for the first time in an HP2P, a large ligand-driven α-domain motion essential to allow substrate access to the active site. This motion recruits residues both downstream of a molecular hinge and on the U-loop to participate in specificity subsites, and mutagenesis identified a mobile lysine residue as a key determinant of positional specificity of the enzyme. Taken together, these data provide important new insights to the factors determining stability, substrate recognition, and the structural mechanism of hydrolysis in this industrially important group of enzymes.

Download the data from Education Week showing how over 900 school districts provided instruction on their first day of the school year.

Missing or inaccessible lung function measurements, gathered over time, have the potential to stagnate or impair clinical care decisions being made. This jeopardises patient safety and often contributes to excessive resource utilisation. Data integration is fundamental to clinical decision-making and entails amalgamating lung function data from multiple sources in a user-friendly format. Despite this, current systems for recording lung function data are suboptimal, with copious gaps in the clinical picture arising from missing or inaccessible lung function measurements. This article discusses the importance of data integration for lung function, with a call to action for key stakeholders involved in the performance, management and interpretation of such tests.

Smart TVs from Samsung and LG monitor what you are watching even when you are using the screens to display a feed from a connected laptop or video game console

Enterprise spending on cloud services continues to go up, up, up — to the tune of $675 billion this year — thanks to organizations’ firm embrace of software-as-a-service, the popularity of distributed working, and the arrival of compute-intensive tech like AI. A startup called PointFive believes it has found a better way to get a […]

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Casual mobile games have reached a fever pitch of interest with consumers, and so too with investors, who are rushing to back the biggest and most promising startups building these quick hits of engagement and diversion. In the latest development, a startup out of London called Tripledot — the company behind a popular Solitaire app, […]

© 2024 TechCrunch. All rights reserved. For personal use only.

The dollar index was up 0.34 per cent at 101.06

House Speaker Nancy Pelosi took a swipe at President Trump as she and fellow Democrats are plowing forward with another massive coronavirus relief package.

High-resolution pictures or atomic-level detail of chikungunya virus interacting with a protein found on the cell surface in the joints was snapped by

In 2015, three economies in China participated in the OECD Programme for International Student Assessment, or PISA, for the first time: Beijing, a municipality, Jiangsu, a province on the eastern coast of the country, and Guangdong, a southern coastal province. Shanghai, which, like Beijing, is also a Chinese megacity of over 20 million people, has participated in PISA since 2009.

Countries have seen a major increase in the educational attainment level of their populations. In 1965, only 43% of young adults aged 25-34 had attained upper secondary education or higher on average across OECD countries. Fifty years later, upper secondary education had almost doubled with attainment levels reaching 84% in 2015.

China has the largest education system in the world. With almost 260 million students and over 15 million teachers in about 514 000 schools (National Bureau of Statistics of China, 2014), excluding graduate education institutions, China’s education system is not only immense but diverse.

The transmembrane intracellular lectin ER–Golgi intermediate compartment protein 53 (ERGIC-53) and the soluble EF-hand multiple coagulation factor deficiency protein 2 (MCFD2) form a complex that functions as a cargo receptor, trafficking various glycoproteins between the endoplasmic reticulum (ER) and the Golgi apparatus. It has been demonstrated that the carbohydrate-recognition domain (CRD) of ERGIC-53 (ERGIC-53CRD) interacts with N-linked glycans on cargo glycoproteins, whereas MCFD2 recognizes polypeptide segments of cargo glycoproteins. Crystal structures of ERGIC-53CRD complexed with MCFD2 and mannosyl oligosaccharides have revealed protein–protein and protein–sugar binding modes. In contrast, the polypeptide-recognition mechanism of MCFD2 remains largely unknown. Here, a 1.60 Å resolution crystal structure of the ERGIC-53CRD–MCFD2 complex is reported, along with three other crystal forms. Comparison of these structures with those previously reported reveal that MCFD2, but not ERGIC-53–CRD, exhibits significant conformational plasticity that may be relevant to its accommodation of various polypeptide ligands.

This article reports conformational polymorphisms of the EF-hand protein MCFD2 which is involved in glycoprotein transport..

A crystallographic indexing algorithm, pinkIndexer, is presented for the analysis of snapshot diffraction patterns. It can be used in a variety of contexts including measurements made with a monochromatic radiation source, a polychromatic source or with radiation of very short wavelength. As such, the algorithm is particularly suited to automated data processing for two emerging measurement techniques for macromolecular structure determination: serial pink-beam X-ray crystallography and serial electron crystallography, which until now lacked reliable programs for analyzing many individual diffraction patterns from crystals of uncorrelated orientation. The algorithm requires approximate knowledge of the unit-cell parameters of the crystal, but not the wavelengths associated with each Bragg spot. The use of pinkIndexer is demonstrated by obtaining 1005 lattices from a published pink-beam serial crystallography data set that had previously yielded 140 indexed lattices. Additionally, in tests on experimental serial crystallography diffraction data recorded with quasi-monochromatic X-rays and with electrons the algorithm indexed more patterns than other programs tested.

Futures in New York fell 2.3 percent Wednesday, after doubling in value from a week ago.