Erythromycin-resistance methyltransferases are SAM dependent Rossmann fold methyltransferases that convert A2058 of 23S rRNA to m6 2A2058. This modification sterically blocks binding of several classes of antibiotics to 23S rRNA, resulting in a multidrug-resistant phenotype in bacteria expressing the enzyme. ErmC is an erythromycin resistance methyltransferase found in many Gram-positive pathogens, whereas ErmE is found in the soil bacterium that biosynthesizes erythromycin. Whether ErmC and ErmE, which possess only 24% sequence identity, use similar structural elements for rRNA substrate recognition and positioning is not known. To investigate this question, we used structural data from related proteins to guide site-saturation mutagenesis of key residues and characterized selected variants by antibiotic susceptibility testing, single turnover kinetics, and RNA affinity–binding assays. We demonstrate that residues in α4, α5, and the α5-α6 linker are essential for methyltransferase function, including an aromatic residue on α4 that likely forms stacking interactions with the substrate adenosine and basic residues in α5 and the α5-α6 linker that likely mediate conformational rearrangements in the protein and cognate rRNA upon interaction. The functional studies led us to a new structural model for the ErmC or ErmE-rRNA complex.

3 July 2020

The Connecticut Coalition for Achievement Now, or ConnCAN, has profiled five successful early childhood education programs in other states for ideas to help programs in Connecticut.

Aggression involves both sexually monomorphic and dimorphic actions. How the brain implements these two types of actions is poorly understood. We found that in Drosophila melanogaster, a set of neurons, which we call CL062, previously shown to mediate male aggression also mediate female aggression. These neurons elicit aggression acutely and without the presence of a target. Although the same set of actions is elicited in males and females, the overall behavior is sexually dimorphic. The CL062 neurons do not express fruitless, a gene required for sexual dimorphism in flies, and expressed by most other neurons important for controlling fly aggression. Connectomic analysis in a female electron microscopy dataset suggests that these neurons have limited connections with fruitless expressing neurons that have been shown to be important for aggression and signal to different descending neurons. Thus, CL062 is part of a monomorphic circuit for aggression that functions parallel to the known dimorphic circuits.

Local people notice the unity in Christ of OM Montenegro as the team work and relax together.

An unexpected connection on a prayer walk leads to multiple opportunities to share spiritual truth with one Muslim family.

After years of work and anticipation, Gypsy Smith has been published in Serbian, a cause for rejoicing in a Roma church in Serbia.

Pankaj Tripathi revealed a surprising ingredient that he adds to his tea to make it special.

Every day, there are tens of thousands of domain names registered across the globe – often as a key first step in creating a unique online presence. Making that experience possible for Verisign-operated top-level domains (TLDs) like .com and .net is a powerful and flexible technology platform first introduced 25 years ago. Thanks to the […]

The post The Verisign Shared Registration System: A 25-Year Retrospective appeared first on Verisign Blog.

Title: Shared Decision-Making Is Better Than Solo
Category: Health News
Created: 8/27/2010 10:03:00 AM
Last Editorial Review: 8/27/2010 10:03:26 AM

It is difficult to disentangle Russian and Chinese scientists from international science cooperation. That is a good thing

"Afghanistan-Pakistan Shared Waters: State of the Basins" was funded by USAID through the Water Management for Enhanced Productivity program, implemented by IWMI.

The post First book on Pak-Afghan shared waters unveiled in Islamabad first appeared on International Water Management Institute (IWMI).

Co-organized by IWMI, experts in water management, agriculturalists, government officials, and business leaders from different parts of the world discussed practical strategies to tackle water scarcity.

The post Pakistan Water Week 2023 concludes with call to collaborate on shared asset first appeared on International Water Management Institute (IWMI).

How shared medlinkmedical appointments/medlink influence patient experience, including knowledge gained, satisfaction, and behavior, such as follow-up

Both corporate travel managers and business travellers in Europe have a shared responsibility as well as opportunities to mitigate risk by making smart choices when it comes to ground transportation for business trips. This is according to a new report, titled Maximising Duty of Care: Choosing the Safest ‘Last Mile’ Option for Your Business Travel from FREENOW, Europe’s multi mobility app with taxi at its core, and the Global Business Travel Association (GBTA), the world’s largest business travel association.

[S.l.] : ARC HUMANITIES PR, 2023.

Your property-related legal queries answered by S.C. RAGHURAM, Partner, RANK Associates, a Chennai-based law firm

robotics 2

It was both sublime and funny verses at The All India Mushaira that captivated the audience.

The state alone cannot undertake the task of protecting India’s first UNESCO-declared biosphere; communities need to get involved

RSS Syndication or RSS Newsfeeds (RSS Feeds for short) all refer to the same thing. There are two parts to the process, the publisher, and the consumer. The publisher produces a small text file in a special format that lists the title and address of an article or resource published on the World Wide Web.

The Japanese megabank and the Singapore-headquartered superapp get to plug gaps in their businesses.

The post How MUFG’s $700m investment in SoftBank-backed Grab is a three-way shared ride appeared first on DealStreetAsia.

Tabu and Aishwarya Rai Bachchan's movie, Kandukondain Kandukondain completed 20 years today and this movie besides being based on Jane Austen's Sense and Sensibility was also a fashion treat. Tabu and Aishwarya Rai looked gorgeous on-screen and gave us

There was certainly was stress in the air when health and economic consqeuences of COVID-19 bore down on Brooklyn and the rest of America. But as these pressures grew, constructive hearts and minds prevailed at the BKLYN Commons (BKC) shared workspace operation at 495 Flatbush Ave., in the Prospect-Lefferts Gardens area, which creatively and passionately morphed to adapt to the new normal.

This final report summarizes and reflects upon the key findings of the Improving EU and U.S. Immigration Systems: Learning from Experience comparative research project undertaken by MPI and the European University Institute through a grant from the European Commission.

Barranquilla, Colombia :: Mission organisations come together on board Logos Hope to inspire Latinos to serve God around the world.

On 1 December 2014 we are expecting legislation to come into force that will give effect to the new shared parental leave regime. The new regime will apply to employees in England, Scotland and Wales whose babies are due, or who will adopt a child, ...

If there’s one word that encapsulates the desires and aspirations of education stakeholders around the world, it is improvement.

This report explores this question on the basis of detailed mobility data including origin, destination and timing of all trips for a mid-sized European city. ITF developed a model to test various alternative transport system configurations that would provide the same level of mobility (locations and timing) as today.

In 2011, TIME Magazine named collaborative consumption (or the sharing economy as it is often called) as one of the top 10 ideas that will change the world. Four years on, this prediction seems to be holding true. The number of companies operating in the sharing economy is rising rapidly in the transport sector alone.

This high-level meeting will be organised for the first time in the Eurasia region, in Almaty, Kazakhstan. This event creates an opportunity to further strengthen relations between the countries of the region and the OECD and serves as a platform for a discussion on a broad spectrum of thematic issues relevant to further improving the region’s competitiveness.

Instagram is now removing coronavirus-related content and accounts from recommendations and 'Explore' option unless it is posted by or belonging to credible health organizations.

When conducting research involving the testing of human biospecimens, investigators and their institutions should routinely consider whether and how to return individual research results on a study-specific basis through an informed decision-making process, says a new report from the National Academies of Sciences, Engineering, and Medicine.

People using a coworking space.; Credit: Cross Campus

In a sign of increased desire of professionals to work remotely, the successful Santa Monica shared workspace Cross Campus is opening a second location in Pasadena later this month, and the company hopes to open eight others in Southern California and beyond in the next two years. 

Dubbed by one user as  the “nerve center” of the Silicon Beach tech scene, Cross Campus opened its membership-based workspace facility in Santa Monica in 2012.   

But co-founder Ronen Olshansky said the shared workspace phenomenon isn't limited to coders. 

"Fewer and fewer people are making the traditional drive into the corporate office," Olshansky said. "They're working remotely as professionals, going off on their own as freelancers, or they're starting their own companies as entrepreneurs."  

A forecast from Forrester Research says that 43 percent of workers will telecommute by 2016, compared to estimates of about a quarter of the workforce telecommuting last year. 

Olshansky said that, for many people, working from home or in a coffee shop isn't productive. 

That's led shared workspaces to pop up in Los Angeles, Culver City and Santa Monica. Among them: Maker City L.A., WeWork, NextSpace, Coloft and Hub LA.  

Los Angeles-based tech investor David Waxman said these kind of shared spaces are crucial for the early stages of tech ventures.

"When you’re just starting out, and capital is very scarce, having not to commit to an entire office but having part of an office is very important," Waxman said.  “There comes a collective energy when a bunch of entrepreneurs get together in the same space, even if they’re not working on the same project."

And he said Pasadena is a good choice for a shared workspace.

"It is the home of Caltech, the Arts Center, and IdeaLab — probably the world’s first tech incubator — started there," he said.

But he said the need isn't limited to Pasadena.

"In Silver Lake, in South Pasadena, in Glendale, you see a lot of little pockets of  people getting together, and as soon as there’s a critical mass, we’ll see co-working spaces like Cross Campus come into being," said Waxman, who named his investment firm TenOneTen after the two freeways that connect Santa Monica and the Westside to Pasadena. 

Alex Maleki of IdeaLab in Pasadena is happy a well-known company is opening up in his city. 

"Anything that helps attract talent and capital to the region," Maleki said, "is absolutely fantastic."

This content is from Southern California Public Radio. View the original story at SCPR.org.

The names, addresses, and telephone numbers of certain FCA complainants were publicly available between November 2019 and February 2020.

Won the award at the SSON's 2019 North American Shared Services Conference.

There are so many different types of hosting that it can be overwhelming to choose the right one for your WordPress site, but at the same time, it just means there are enough options so you can choose the perfect fit.

Assistive shared-control robots have the potential to transform the lives of millions of people afflicted with severe motor impairments. The usefulness of shared-control robots typically relies on the underlying autonomy's ability to infer the user's needs and intentions, and the ability to do so unambiguously is often a limiting factor for providing appropriate assistance confidently and accurately. The contributions of this paper are four-fold. First, we introduce the idea of intent disambiguation via control mode selection, and present a mathematical formalism for the same. Second, we develop a control mode selection algorithm which selects the control mode in which the user-initiated motion helps the autonomy to maximally disambiguate user intent. Third, we present a pilot study with eight subjects to evaluate the efficacy of the disambiguation algorithm. Our results suggest that the disambiguation system (a) helps to significantly reduce task effort, as measured by number of button presses, and (b) is of greater utility for more limited control interfaces and more complex tasks. We also observe that (c) subjects demonstrated a wide range of disambiguation request behaviors, with the common thread of concentrating requests early in the execution. As our last contribution, we introduce a novel field-theoretic approach to intent inference inspired by dynamic field theory that works in tandem with the disambiguation scheme.

Assistive robots enable people with disabilities to conduct everyday tasks on their own. However, these tasks can be complex, containing both coarse reaching motions and fine-grained manipulation. For example, when eating, not only does one need to move to the correct food item, but they must also precisely manipulate the food in different ways (e.g., cutting, stabbing, scooping). Shared autonomy methods make robot teleoperation safer and more precise by arbitrating user inputs with robot controls. However, these works have focused mainly on the high-level task of reaching a goal from a discrete set, while largely ignoring manipulation of objects at that goal. Meanwhile, dimensionality reduction techniques for teleoperation map useful high-dimensional robot actions into an intuitive low-dimensional controller, but it is unclear if these methods can achieve the requisite precision for tasks like eating. Our insight is that---by combining intuitive embeddings from learned latent actions with robotic assistance from shared autonomy---we can enable precise assistive manipulation. In this work, we adopt learned latent actions for shared autonomy by proposing a new model structure that changes the meaning of the human's input based on the robot's confidence of the goal. We show convergence bounds on the robot's distance to the most likely goal, and develop a training procedure to learn a controller that is able to move between goals even in the presence of shared autonomy. We evaluate our method in simulations and an eating user study.

A system and method for detecting, filtering, prioritizing and reporting shared memory hazards are disclosed. The method includes, for a unit of hardware operating on a block of threads, mapping a plurality of shared memory locations assigned to the unit to a tracking table. The tracking table comprises initialization information for each shared memory location. The method also includes, for an instruction of a program within a barrier region, identifying a potential conflict by identifying a second access to a location in shared memory within a block of threads executed by the hardware unit. First information associated with a first access and second information associated with the second access to the location is determined. Filter criteria is applied to the first and second information to determine whether the instruction causes a reportable hazard. The instruction is reported when it causes the reportable hazard.

Systems, methods and computer program products may provide managing utilization of one or more physical processors in a shared processor pool. A method of managing utilization of one or more physical processors in a shared processor pool may include determining a current amount of utilization of the one or more physical processors and generating an instruction message. The instruction message may be at least partially determined by the current amount of utilization. The method may further include sending the instruction message to a guest operating system, the guest operating system having a number of enabled virtual processors.

The technology of the present application provides a networked computer system with at least one workstation and at least one shared resource such as a database. Access to the database by the workstation is managed by a database management system. An access engine reviews job requests for access to the database and allows job requests access to the resource based protocols stored by the system.

An array of a plurality of processing elements (PEs) are in a data packet-switched network interconnecting the PEs and memory to enable any of the PEs to access the memory. The network connects the PEs and their local memories to a common controller. The common controller may include a shared load/store (SLS) unit and an array control unit. A shared read may be addressed to an external device via the common controller. The SLS unit can continue activity as if a normal shared read operation has taken place, except that the transactions that have been sent externally may take more cycles to complete than the local shared reads. Hence, a number of transaction-enabled flags may not have been deactivated even though there is no more bus activity. The SLS unit can use this state to indicate to the array control unit that a thread switch may now take place.

A speech recognition system, method of recognizing speech and a computer program product therefor. A client device identified with a context for an associated user selectively streams audio to a provider computer, e.g., a cloud computer. Speech recognition receives streaming audio, maps utterances to specific textual candidates and determines a likelihood of a correct match for each mapped textual candidate. A context model selectively winnows candidate to resolve recognition ambiguity according to context whenever multiple textual candidates are recognized as potential matches for the same mapped utterance. Matches are used to update the context model, which may be used for multiple users in the same context.