The US-India Clean Energy Partnership and Quad Provide Overlapping Pathways to Produce Clean Energy The US-India Clean Energy Partnership and Quad Provide Overlapping Pathways to Produce Clean Energy

Abingdon, Oxon ; New York, NY : Routledge , 2024.

Extracytoplasmic sugar decoration of glycopolymer components of the bacterial cell wall contributes to their structural diversity. Typically, the molecular mechanism that underpins such a decoration process involves a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer. Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall–associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis. On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates. Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA). We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs. Finally, we observed that glucosylation of both rhamnan and PSP can increase resistance to bacteriophage predation and that LTA galactosylation alters L. lactis resistance to bacteriocin.

A toxin isolated from the Togo starburst tarantula provides new insights into pain mechanisms and could lead to new treatments for irritable bowel syndrome

With their large, hairy bodies and long legs, tarantulas are an arachnophobe’s worst nightmare. For pain researchers, however, these outsized spiders are a dream come true: Their venom contains a cocktail of toxins, each of which activates pain-sensing nerve fibres in different ways, and researchers in the United States have now identified one such toxin that will help them to better understand pain, and could also lead to treatments for the chronic pain associated with irritable bowel syndrome.

Physical pain signals are transmitted from the body to the brain by specialised sensory neurons called nociceptors. These pain-sensing neurons have cell bodies located just outside the spinal cord, and possess a single conductive fibre that splits in two, with one branch extending out towards the skin surface, and the shorter one entering the back of the cord.

Related: Uncomfortably numb: The people who feel no pain

Related: Researchers identify gatekeeper neurons that control pain and itch

This edition presents an overview of some “alternative pathways” that could help take the pressure off the main traditional pathways for refugees in general and assesses their potential application for Syrians in particular. Overall, these alternatives can help provide safe channels and good integration prospects to refugees who might otherwise be tempted to risk their fate with smugglers and illegal border crossing.

This report examines what would be needed to achieve zero CO2 emissions from international maritime transport by 2035. It assesses measures that can reduce shipping emissions effectively and describes possible decarbonisation pathways that use different combinations of these measures; and reviews under which conditions these measures could be implemented and presents concrete policy recommendations.

This report offers a guide on potential reform pathways towards sustainable agriculture water use, based on a thorough review of selected past water and agriculture reforms and extensive consultation with policy experts.

Skills and educational development for inclusive and sustainable growth are becoming significant drivers in OECD countries.

Over the last generation, it has become clear that something has gone awry in how the United States prepares its young people for life. In spite of millions of young people pursuing university education, fewer than one in three young Americans successfully attain a bachelor’s degree, while millions of good middle-skills jobs go begging.

Digitisation is expected to profoundly change the way we learn and work – at a faster pace than previous major drivers of transformation. Many children entering school today are likely to end up working in jobs that do not yet exist.

Skills and educational development for inclusive and sustainable growth are becoming significant drivers in OECD countries.

This guidance document intends to provide an insight into which pieces of information that are necessary to identify and document an adverse outcome pathway (AOP) and how to present them. The AOP concept has been developed as a means of providing transparent mechanistic justification and weight-of-evidence to reduce uncertainty in the predictions for complex toxicological endpoints.

The OECD is pleased to announce, together with the U.S Environmental Protection Agency and the European Commission Joint Research Centre, the Adverse Outcome Pathway Knowledge Base (AOP KB). This is a web-based platform which aims to bring together all the knowledge on how chemicals can induce adverse effects, therefore providing a focal point for AOP development and dissemination.

Latest releases: AOPs are the central element of a toxicological knowledge framework, promoted by member countries through OECD, built to support chemical risk assessment based on mechanistic reasoning. Seven New AOPs have now been published.

A combined biophysical approach was applied to map gas-docking sites within murine neuroglobin (Ngb), revealing snapshots of events that might govern activity and dynamics in this unique hexacoordinate globin, which is most likely to be involved in gas-sensing in the central nervous system and for which a precise mechanism of action remains to be elucidated. The application of UV–visible microspectroscopy in crystallo, solution X-ray absorption near-edge spectroscopy and X-ray diffraction experiments at 15–40 K provided the structural characterization of an Ngb photolytic intermediate by cryo-trapping and allowed direct observation of the relocation of carbon monoxide within the distal heme pocket after photodissociation. Moreover, X-ray diffraction at 100 K under a high pressure of dioxygen, a physiological ligand of Ngb, unravelled the existence of a storage site for O2 in Ngb which coincides with Xe-III, a previously described docking site for xenon or krypton. Notably, no other secondary sites were observed under our experimental conditions.

Wednesday, May 13, 2020, 1 – 2pm

Online Event

If you want a career in healthcare, Pathways are a great place to start. Pathways are FREE 5-12 week training programs that will prepare you for specific occupations in the healthcare industry. Employer partnerships are a key part of the programs and will connect you with multiple job opportunities in the healthcare sector.
Current programs available include Nursing Assistant, Medical Administration, Pharmacy Tech, Medical Call Center and Medical Office Support.

Join us on May 13th from 1-2pm to learn about upcoming Pathways in the Twin Cities and how to apply!

Hosted by Minneapolis College in coordination with the City of Minneapolis Health Hire.

Event type: Job and Career Fairs
Filter by Location: Twin Cities Metro
Event Audience: For Job Seekers, For Veterans
Registration link: www.eventbrite.com…
Accommodation Request: 612-673-5187
CareerForce Sponsored Event: CareerForce sponsored event

Unification and Integration is key in the fragmented digital health ecosystem

The ONU Global Salesforce Pathways micro-credential recognizes knowledge of and experience in Salesforce with an academic credential and three college credits.

Courses now available to thousands of middle and high schools making learning how to code and gaining multiple Microsoft Technology Associate Certifications (MTA) a lot easier.

The Middle East Institute explores "Pathways to a Sustainable US-Pakistan Relationship" in discussion with Ali Jehangir Siddiqui

The Centers for Medicare & Medicaid Services (CMS) is taking a new direction with the Medicare Shared Savings Program, established by the Affordable Care Act. The new ruling, called Pathways to Success, is meant to encourage Medicare’s Accountable Care Organizations… Read More

The post CMS Creates Pathways to Success for ACOs Starting July 1, 2019 appeared first on Anders CPAs.

The pandemic has changed the daily lives of everyone. How we work, how we shop, and how we interact with each other are all shifting. Comparing life as it is now with how it used to be can lead to sadness or despair and what's called "ambiguous loss."

The pandemic has changed the daily lives of everyone. How we work, how we shop, and how we interact with each other are all shifting. Comparing life as it is now with how it used to be can lead to sadness or despair and what's called "ambiguous loss."

The methods described herein enable the evaluation of compounds on subjects to assess their therapeutic efficacy or toxic effects. The target of analysis is the underlying biochemical process or processes (i.e., metabolic process) thought to be involved in disease pathogenesis. Molecular flux rates within the one or more biochemical processes serve as biomarkers and are quantitated and compared with the molecular flux rates (i.e., biomarker) from control subjects (i.e., subjects not exposed to the compounds). Any change in the biomarker in the subject relative to the biomarker in the control subject provides information to evaluate therapeutic efficacy of an administered drug or a toxic effect and to develop the compound further if desired. In one aspect of the invention, stable isotope-labeled substrate molecules are administered to a subject and the label is incorporated into targeted molecules in a manner that reveals molecular flux rates through metabolic pathways of interest.

Organ shortage continues to limit the lives of patients who require liver transplantation. While extending criteria for liver organs provides a needed resource, tissue damage from prolonged ischemic injury can result in early allograft dysfunction and consequent rejection. In this issue of the JCI, Nakamura et al. used a mouse transplantation model with prolonged ex vivo cold storage to explore liver graft protection. The authors found that liver grafts with absent carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) exhibited increased ischemia-reperfusion injury inflammation and decreased function in wild-type recipients. The authors went on to correlate CEACAM1 levels with postreperfusion damage in human liver transplant recipients. Notably, this study identified a potential biomarker for liver transplant donor graft quality.

Invitation Only Research Event

Event participants

Usama Otman Essed, Libya Center for Strategic & Future Studies
Chair: Tim Eaton, Middle East and North Africa Programme, Chatham House

Extracytoplasmic sugar decoration of glycopolymer components of the bacterial cell wall contributes to their structural diversity. Typically, the molecular mechanism that underpins such a decoration process involves a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer. Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall–associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis. On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates. Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA). We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs. Finally, we observed that glucosylation of both rhamnan and PSP can increase resistance to bacteriophage predation and that LTA galactosylation alters L. lactis resistance to bacteriocin.

Robert W. Mahley
Jan 1, 1999; 40:1-16
Reviews

Lipid rafts are highly ordered regions of the plasma membrane that are enriched in cholesterol and sphingolipids and play important roles in many cells. In hematopoietic stem and progenitor cells (HSPCs), lipid rafts house receptors critical for normal hematopoiesis. Lipid rafts also can bind and sequester kinases that induce negative feedback pathways to limit proliferative cytokine receptor cycling back to the cell membrane. Modulation of lipid rafts occurs through an array of mechanisms, with optimal cholesterol efflux one of the major regulators. As such, cholesterol homeostasis also regulates hematopoiesis. Increased lipid raft content, which occurs in response to changes in cholesterol efflux in the membrane, can result in prolonged receptor occupancy in the cell membrane and enhanced signaling. In addition, certain diseases, like diabetes, may contribute to lipid raft formation and affect cholesterol retention in rafts. In this review, we explore the role of lipid raft-related mechanisms in hematopoiesis and CVD (specifically, atherosclerosis) and discuss how defective cholesterol efflux pathways in HSPCs contribute to expansion of lipid rafts, thereby promoting myelopoiesis and thrombopoiesis. We also discuss the utility of cholesterol acceptors in contributing to lipid raft regulation and disruption, and highlight the potential to manipulate these pathways for therapeutic gain in CVD as well as other disorders with aberrant hematopoiesis.

Van Wedeen talks about the geometry of the brain's communication pathways.

Extracytoplasmic sugar decoration of glycopolymer components of the bacterial cell wall contributes to their structural diversity. Typically, the molecular mechanism that underpins such a decoration process involves a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer. Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall–associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis. On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates. Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA). We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs. Finally, we observed that glucosylation of both rhamnan and PSP can increase resistance to bacteriophage predation and that LTA galactosylation alters L. lactis resistance to bacteriocin.

Taewook Kang
Apr 7, 2020; 0:RA119.001882v1-mcp.RA119.001882
Research

Invitation Only Research Event

Event participants

Usama Otman Essed, Libya Center for Strategic & Future Studies
Chair: Tim Eaton, Middle East and North Africa Programme, Chatham House