Fenix's first game of the season on September 21 ended with a 19-0 defeat. But for its fans and players, trans men having the right to play their favourite sport on equal terms is far more important than the score.

Thousands of people have visited the clinic since its launch in 2022 thanks to friendly doctors; those coming from outstations find it difficult to make multiple trips; they also want a hostel for those visiting the clinic since they are not welcome at lodges, says an activist

When a famed Egyptian actor revealed his daughter had transitioned, the reaction was unconventional.

β-Glucocerebrosidase (GBA) hydrolyzes glucosylceramide (GlcCer) to generate ceramide. Previously, we demonstrated that lysosomal GBA1 and nonlysosomal GBA2 possess not only GlcCer hydrolase activity, but also transglucosylation activity to transfer the glucose residue from GlcCer to cholesterol to form β-cholesterylglucoside (β-GlcChol) in vitro. β-GlcChol is a member of sterylglycosides present in diverse species. How GBA1 and GBA2 mediate β-GlcChol metabolism in the brain is unknown. Here, we purified and characterized sterylglycosides from rodent and fish brains. Although glucose is thought to be the sole carbohydrate component of sterylglycosides in vertebrates, structural analysis of rat brain sterylglycosides revealed the presence of galactosylated cholesterol (β-GalChol), in addition to β-GlcChol. Analyses of brain tissues from GBA2-deficient mice and GBA1- and/or GBA2-deficient Japanese rice fish (Oryzias latipes) revealed that GBA1 and GBA2 are responsible for β-GlcChol degradation and formation, respectively, and that both GBA1 and GBA2 are responsible for β-GalChol formation. Liquid chromatography–tandem MS revealed that β-GlcChol and β-GalChol are present throughout development from embryo to adult in the mouse brain. We found that β-GalChol expression depends on galactosylceramide (GalCer), and developmental onset of β-GalChol biosynthesis appeared to be during myelination. We also found that β-GlcChol and β-GalChol are secreted from neurons and glial cells in association with exosomes. In vitro enzyme assays confirmed that GBA1 and GBA2 have transgalactosylation activity to transfer the galactose residue from GalCer to cholesterol to form β-GalChol. This is the first report of the existence of β-GalChol in vertebrates and how β-GlcChol and β-GalChol are formed in the brain.

I'll try to keep this short: So I have always dated guys. Yet when I am not dating guys, my best friend and I; who's a girl, hook up. We've been doing this for about 5 years, we met at the age of 15, and 5 yrs later I had my first lesbian experience with her, and then 5 years it became a fairly regular thing. 5 years is obviously a long time. But when I'm not dating a guy, I always go back to her. And she's the only woman I have ever been with. That's why I am confused about this. I do not know if I'm straight or a lesbian?

sir on dated 4 th January 2020 I have a baby child born ,after 2 weeks some transgender came to my home and demanding huge mone a sums of 30000 and when I had refused to give then are using slang languages and doing mischevious activities
like doing toilet infront of my home,harassing us .sir what are punishement according to Indian law for Them,please tell so that from next time I will take the steps against for them.
from Kolkata

Pension Managers Conference, Torquay, 24-25 November 2009 Further to several requests for details of the cases referred to in Gary Delderfield’s talk at the Conference, this special LGPS Speedbrief contains links to all the cases mentioned in ...

This Biosafety document aims to serve as an aid to risk assessors and regulators; providing guidance on handling the aspects of an environmental safety assessment, accessing and using information in situations of low-level presence (LLP) of transgenic plants in seed and grain commodities.

Volume I of this series compiles the science-based consensus documents of the OECD Task Force for the Safety of Novel Foods and Feeds from 2002 to 2008.

Official figures show for the first time the true scale of offending by criminals who were born male but were allowed to move into female jails after changing gender. Convicted rapist Karen White pictured.

Official figures show for the first time the true scale of offending by criminals who were born male but were allowed to move into female jails after changing gender. Convicted rapist Karen White pictured.

The Gender Guardian offers 12 years of education, from elementary school to college.

The Justice Department announced today that Juan Carlos Pagán Bonilla (Pagan), 21, and Sean Díaz de León (Diaz), 19, have been charged by a criminal complaint in federal district court with carjacking and with using and carrying a firearm during and in relation to a crime of violence in connection with the death of two transgender individuals. Pagan and Diaz are now in federal custody.

Digital watermarking is a remarkable issue in the field of information security to avoid the misuse of images in multimedia networks. Although access to unauthorized persons can be prevented through cryptography, it cannot be simultaneously used for copyright protection or content authentication with the preservation of image integrity. Hence, this paper presents an optimized multipurpose blind watermarking in Shearlet domain with the help of smart algorithms including MLP and NSGA-II. In this method, four copies of the robust copyright logo are embedded in the approximate coefficients of Shearlet by using an effective quantization technique. Furthermore, an embedded random sequence as a semi-fragile authentication mark is effectively extracted from details by the neural network. Due to performing an effective optimization algorithm for selecting optimum embedding thresholds, and also distinguishing the texture of blocks, the imperceptibility and robustness have been preserved. The experimental results reveal the superiority of the scheme with regard to the quality of watermarked images and robustness against hybrid attacks over other state-of-the-art schemes. The average PSNR and SSIM of the dual watermarked images are 38 dB and 0.95, respectively; Besides, it can effectively extract the copyright logo and locates forgery regions under severe attacks with satisfactory accuracy.

The present invention provides transgenic soybean event MON87769, and cells, seeds, and plants comprising DNA diagnostic for the soybean event. The invention also provides compositions comprising nucleotide sequences that are diagnostic for said soybean event in a sample, methods for detecting the presence of said soybean event nucleotide sequences in a sample, probes and primers for use in detecting nucleotide sequences that are diagnostic for the presence of said soybean event in a sample, growing the seeds of such soybean event into soybean plants, and breeding to produce soybean plants comprising DNA diagnostic for the soybean event.

SIR Ian Botham believes Hampshire chairman Rod Bransgrove is more deserving of a knighthood than Sir Geoffrey Boycott.

The videos in question, which are taken during what looks like a photo shoot, show the actress/reality TV star appearing to have a bulge underneath her form-fitting jeans.

Jetzt ist es offiziell: In der 1. und 2. Bundesliga wird der Ball wieder am 16. Mai rollen. Dies verkündete DFL-Geschäftsführer Christian Seifert auf einer Pressekonferenz.

Why does the Australian Tax Office require people to tick a mental illness box for early access to their superannuation to fund gender transition surgery?

The Victorian Government prepares to reintroduce a bill which would make it easier for people to alter the sex recorded on their birth certificate to male, female or any other gender descriptor of their choice.

Victorian Greens Senator Janet Rice announces her wife, renowned climate scientist and transgender woman Penny Whetton, has died suddenly at their home in northern Tasmania.

The Sutherlands hope that telling their story will help transgender parents be accepted to the point where, one day, "no-one bats an eyelid".

The eight episode, half-hour series will be written by showrunner Alexandra Rushfield and will be produced by Rogen's Point Grey Pictures as part of their multiplatform partnership with Lionsgate.

On Sunday, May 3rd, the body of 28-year-old transgender woman Nina Pop was found in her apartment in Sikestown, Missouri. She had been stabbed multiple times, according to police. While police have not determined a motive, they are looking into the possibility of a hate crime. The LGBTQ advocacy group Human Rights Campaign reported that Pop’s murder is at least the 10th violent death of a transgender or gender nonconforming person this year, and the fifth in the past month. All five of the recent victims were transgender women of color. Tori Cooper, director of community engagement for HRC’s Transgender Justice Initiative, wrote in a blog post, “for the past four weeks, we have seen the deaths of five transgender women of color in this country. We are seeing an epidemic of violence that can no longer be ignored. Transgender and gender-nonconforming people, especially trans women of color, risk our lives by living as our true selves — and we are being violently killed for doing so”. Transgender and gender nonconforming people lack expansive, explicit federal legal protections to safeguard against the vast discrimination they receive. While they are covered under the state’s hate crimes legislation, they are not explicitly […]

A transgender woman awaiting trial on charges that she swindled elderly women out of their savings was raped repeatedly in jail after she was housed against her will with rowdy male inmates, according to a new lawsuit.

A regular in Fox's '9-1-1: Lone Star,' Smith has gotten a lot of reading done as he waits out the pandemic in a friend's guestroom.

The “Saved by the Bell” star later apologized for stirring controversy in a resurfaced interview with conservative Candace Owens.

Assembled α-synuclein in nerve cells and glial cells is the defining pathological feature of neurodegenerative diseases called synucleinopathies. Seeds of α-synuclein can induce the assembly of monomeric protein. Here, we used sucrose gradient centrifugation and transiently transfected HEK 293T cells to identify the species of α-synuclein from the brains of homozygous, symptomatic mice transgenic for human mutant A53T α-synuclein (line M83) that seed aggregation. The most potent fractions contained Sarkosyl-insoluble assemblies enriched in filaments. We also analyzed six cases of idiopathic Parkinson's disease (PD), one case of familial PD, and six cases of multiple system atrophy (MSA) for their ability to induce α-synuclein aggregation. The MSA samples were more potent than those of idiopathic PD in seeding aggregation. We found that following sucrose gradient centrifugation, the most seed-competent fractions from PD and MSA brains are those that contain Sarkosyl-insoluble α-synuclein. The fractions differed between PD and MSA, consistent with the presence of distinct conformers of assembled α-synuclein in these different samples. We conclude that α-synuclein filaments are the main driving force for amplification and propagation of pathology in synucleinopathies.

Assembled α-synuclein in nerve cells and glial cells is the defining pathological feature of neurodegenerative diseases called synucleinopathies. Seeds of α-synuclein can induce the assembly of monomeric protein. Here, we used sucrose gradient centrifugation and transiently transfected HEK 293T cells to identify the species of α-synuclein from the brains of homozygous, symptomatic mice transgenic for human mutant A53T α-synuclein (line M83) that seed aggregation. The most potent fractions contained Sarkosyl-insoluble assemblies enriched in filaments. We also analyzed six cases of idiopathic Parkinson's disease (PD), one case of familial PD, and six cases of multiple system atrophy (MSA) for their ability to induce α-synuclein aggregation. The MSA samples were more potent than those of idiopathic PD in seeding aggregation. We found that following sucrose gradient centrifugation, the most seed-competent fractions from PD and MSA brains are those that contain Sarkosyl-insoluble α-synuclein. The fractions differed between PD and MSA, consistent with the presence of distinct conformers of assembled α-synuclein in these different samples. We conclude that α-synuclein filaments are the main driving force for amplification and propagation of pathology in synucleinopathies.

β-Glucocerebrosidase (GBA) hydrolyzes glucosylceramide (GlcCer) to generate ceramide. Previously, we demonstrated that lysosomal GBA1 and nonlysosomal GBA2 possess not only GlcCer hydrolase activity, but also transglucosylation activity to transfer the glucose residue from GlcCer to cholesterol to form β-cholesterylglucoside (β-GlcChol) in vitro. β-GlcChol is a member of sterylglycosides present in diverse species. How GBA1 and GBA2 mediate β-GlcChol metabolism in the brain is unknown. Here, we purified and characterized sterylglycosides from rodent and fish brains. Although glucose is thought to be the sole carbohydrate component of sterylglycosides in vertebrates, structural analysis of rat brain sterylglycosides revealed the presence of galactosylated cholesterol (β-GalChol), in addition to β-GlcChol. Analyses of brain tissues from GBA2-deficient mice and GBA1- and/or GBA2-deficient Japanese rice fish (Oryzias latipes) revealed that GBA1 and GBA2 are responsible for β-GlcChol degradation and formation, respectively, and that both GBA1 and GBA2 are responsible for β-GalChol formation. Liquid chromatography–tandem MS revealed that β-GlcChol and β-GalChol are present throughout development from embryo to adult in the mouse brain. We found that β-GalChol expression depends on galactosylceramide (GalCer), and developmental onset of β-GalChol biosynthesis appeared to be during myelination. We also found that β-GlcChol and β-GalChol are secreted from neurons and glial cells in association with exosomes. In vitro enzyme assays confirmed that GBA1 and GBA2 have transgalactosylation activity to transfer the galactose residue from GalCer to cholesterol to form β-GalChol. This is the first report of the existence of β-GalChol in vertebrates and how β-GlcChol and β-GalChol are formed in the brain.

β-Glucocerebrosidase (GBA) hydrolyzes glucosylceramide (GlcCer) to generate ceramide. Previously, we demonstrated that lysosomal GBA1 and nonlysosomal GBA2 possess not only GlcCer hydrolase activity, but also transglucosylation activity to transfer the glucose residue from GlcCer to cholesterol to form β-cholesterylglucoside (β-GlcChol) in vitro. β-GlcChol is a member of sterylglycosides present in diverse species. How GBA1 and GBA2 mediate β-GlcChol metabolism in the brain is unknown. Here, we purified and characterized sterylglycosides from rodent and fish brains. Although glucose is thought to be the sole carbohydrate component of sterylglycosides in vertebrates, structural analysis of rat brain sterylglycosides revealed the presence of galactosylated cholesterol (β-GalChol), in addition to β-GlcChol. Analyses of brain tissues from GBA2-deficient mice and GBA1- and/or GBA2-deficient Japanese rice fish (Oryzias latipes) revealed that GBA1 and GBA2 are responsible for β-GlcChol degradation and formation, respectively, and that both GBA1 and GBA2 are responsible for β-GalChol formation. Liquid chromatography–tandem MS revealed that β-GlcChol and β-GalChol are present throughout development from embryo to adult in the mouse brain. We found that β-GalChol expression depends on galactosylceramide (GalCer), and developmental onset of β-GalChol biosynthesis appeared to be during myelination. We also found that β-GlcChol and β-GalChol are secreted from neurons and glial cells in association with exosomes. In vitro enzyme assays confirmed that GBA1 and GBA2 have transgalactosylation activity to transfer the galactose residue from GalCer to cholesterol to form β-GalChol. This is the first report of the existence of β-GalChol in vertebrates and how β-GlcChol and β-GalChol are formed in the brain.

OBJECTIVE

The long-term influences of sex hormone administration on insulin sensitivity and incretin hormones are controversial. We investigated these effects in 35 transgender men (TM) and 55 transgender women (TW) from the European Network for the Investigation of Gender Incongruence (ENIGI) study.

The Trump administration weighed into a lawsuit, arguing that a state's transgender-athlete policy forces "biological girls to compete against biological boys who publicly identify with the female gender and want to compete on sex-specific athletic teams."

A federal appeals court rejected a multi-pronged challenge to a school district plan allowing transgender students to use restrooms and other facilities that match their gender identity.

Idaho became the first state in the country to prohibit transgender girls from participating in girls' school sports after Gov. Brad Little, a Republican, signed the "Fairness in Women's Sports Act" into law Tuesday.

Châtillon, France : Association Hommefleur, [date of publication not identified]

Lennart Mucke
Jun 1, 2000; 20:4050-4058
Cellular

MECP2 gain-of-function and loss-of-function in genetically engineered monkeys recapitulates typical phenotypes in patients with autism, yet where MECP2 mutation affects the monkey brain and whether/how it relates to autism pathology remain unknown. Here we report a combination of gene–circuit–behavior analyses including MECP2 coexpression network, locomotive and cognitive behaviors, and EEG and fMRI findings in 5 MECP2 overexpressed monkeys (Macaca fascicularis; 3 females) and 20 wild-type monkeys (Macaca fascicularis; 11 females). Whole-genome expression analysis revealed MECP2 coexpressed genes significantly enriched in GABA-related signaling pathways, whereby reduced β-synchronization within fronto-parieto-occipital networks was associated with abnormal locomotive behaviors. Meanwhile, MECP2-induced hyperconnectivity in prefrontal and cingulate networks accounted for regressive deficits in reversal learning tasks. Furthermore, we stratified a cohort of 49 patients with autism and 72 healthy controls of 1112 subjects using functional connectivity patterns, and identified dysconnectivity profiles similar to those in monkeys. By establishing a circuit-based construct link between genetically defined models and stratified patients, these results pave new avenues to deconstruct clinical heterogeneity and advance accurate diagnosis in psychiatric disorders.

SIGNIFICANCE STATEMENT Autism spectrum disorder (ASD) is a complex disorder with co-occurring symptoms caused by multiple genetic variations and brain circuit abnormalities. To dissect the gene–circuit–behavior causal chain underlying ASD, animal models are established by manipulating causative genes such as MECP2. However, it is unknown whether such models have captured any circuit-level pathology in ASD patients, as demonstrated by human brain imaging studies. Here, we use transgenic macaques to examine the causal effect of MECP2 overexpression on gene coexpression, brain circuits, and behaviors. For the first time, we demonstrate that the circuit abnormalities linked to MECP2 and autism-like traits in the monkeys can be mapped to a homogeneous ASD subgroup, thereby offering a new strategy to deconstruct clinical heterogeneity in ASD.

Supporters of the bill say it would extend critical civil rights protections to more students. But opponents say it ignores parents' rights in schools and could lead to confusing situations for some children.

The Trump administration's move to narrowly define gender according to sex assigned at birth could impact transgender students and schools' policies with respect to their rights.

The Education Department's office for civil rights will investigate whether a Georgia elementary school's policy for transgender students contributed to the alleged assault of a girl.