Dr. Holstein is an award winning Selfie Filmmaker, Positive Psychologist and podcaster who helps young people overcome anxiety and alienation through multiple media channels.

Dr. Holstein is an award winning Selfie Filmmaker, Positive Psychologist and podcaster who helps us overcome anxiety and alienation through multiple media channels.

Dr. Claudia Rozuk, M.D. has been recognized by the International Association of Who's Who with the Albert Einstein Award of Medicine.

Designer Jonathan Marc Stein announces the debut of his latest collection at Arab Fashion Week in Dubai, October 2019 for S/S2020 RTW

Part of the joy in breeding or owning horses is choosing their names. J.J. Hysell of In The Money notes that owners of Einstein offspring have been having fun thinking up clever names for their horses. Einstein stands at Adena Springs Farm for a 2013 fee of $7,500. A few of our favorites include: E […]

The post Relatively Clever: Owners Having Fun Naming Einstein Offspring appeared first on Horse Racing News | Paulick Report.

So, there’s this new study doing the rounds. Some economists decided to study the twitter followers of prominent coronavirus skeptics and fearmongers, and it seems that followers of Nassim Taleb were more likely to shelter in place, and less like to die of coronavirus, than followers of Richard Epstein or Cass Sunstein. And the differences […]

Kyoto University researchers said the vaccine for Alzheimer’s was effective and was also shown to have zero side effects observed in the mice.

The post New Alzheimer’s Nasal Spray Shown to Reduce Proteins Which Cause the Disease in Mice appeared first on Good News Network.

After a year as DANGERBIRD RECORDS VP/Promotion, ROZE BRAUNSTEIN is out and about and on a walkabout looking for her next promotion, management or marketing opportunity. ROZE's impressive … more

I’m happy to report 2017 was a much better year than 2016 on many fronts. Family was healthy, we avoided experiencing the passing of family members, there was a fair amount of travel, a few great photos were taken and most importantly to me my boys continue to experience nature and ask for repeat camping trips. In past years yearly recaps I’ve emphasized how much of my focus has shifted from taking nature photos to instilling a sense of wonder about and deep respect for nature in my two young sons. That continues to be the case.   I’ve never delved into it too deeply in past yearly recaps why my prioritization has shifted. In part it’s likely very obvious for some. That said I do want to call it out. I became a photographer because of my love for nature. If I hadn’t been so intrigued by nature I’d never have had a reason to pick up a camera. With the onset of social media and mobile phones with cameras I’m not sure that can be said of many young photographers today. More and more as I view photography online I wonder whether people are truly interested in their subjects (all styles of photography not just nature) or just trying to increase their follower stats because that’s the thing to do in this day and age. Perhaps my cynicism is getting the better of me after working at Borrowlenses where I dealt with photography talking heads & professional and aspiring professional photographers on a daily basis. An element of my becoming a jaded photographer I’m sure (kudos to my friend Richard Wong for writing that post). I’ll avoid going down the path of airing dirt for now as that’s a series of posts unto itself. Anyhow once my boys got old enough it was quite natural for me to want to go back to my roots enjoying nature over photography. I’d like my children to deeply respect and value our natural world. What would I be teaching them, about nature and in general, if I was always taking photos on our trips versus living the moment? For that reason my yearly recap photos have and will continue to be split between family photos and everything else. While my boys are in their most formative years I’ll continue to put my emphasis behind experiencing nature versus artistically capturing it. The fact that my boys regularly tell me, often out of the blue, they want to go camping again means the world to me. I’m incredibly grateful one aspect of my professional outdoor photographer mind has not lost a beat and that is timing our trips for optimal conditions. This used to torment me as I’d leave my DSLR behind, but the more my boys reminisce and ask for more nature experiences the more I know I’ve made the right call. Now that that’s out of the way… my favorite photos, in no particular order are below. Enjoy and thanks for taking the time to view this post and my blog in general.  If you happen to have a recap of your best photos of 2017 I invite you to submit it to my blog project, where I list the best photos of photographers who read my blog. It’s a lot of fun and a great way to get inspired at the beginning of the new year. Best of  Landscape & Nature I took my boys to witness the total solar eclipse in August of 2017. We went off road to an isolated mountaintop with 360 degree views in eastern Oregon. My focus for the eclipse itself was on the experience with my boys, but we did star gaze a lot at night before the big day. In trying to explain how the earth moves I set up a quick star trail sequence to show the boys. This was the result. Water levels were near record levels this spring in Yosemite Valley. I made what has become a regular hike to Upper Yosemite Falls to take time-lapses of moonbows with my friend, Brian Hawkins. Like me, he seems to have enough screws loose to think this night hike is a good idea along with huddling in ice cold mist to capture this view. At the beginning of the year I timed a trip to Yosemite Valley to capture some long overdue winter star trail photos. This was a single 2+ hour exposure taken with my Canon 5D Mark II. Pictured is Three Brothers with star trails reflected in the Merced River. Another single 2+ hour exposure taken with my Canon 5D Mark II. Pictured is Upper Yosemite Falls as seen from the valley floor. For most of my family trips this year I’ve rented an SUV outfitted with a rooftop tent via PacificOverlander. I took this photo on the first night of one of my trips with the boys as we explored the Owens Valley. While they slept I snuck in this photo of our truck/tent under the Milky Way. If you’re unfamiliar with PacificOverlander it’s well worth investigating. I’ve had several amazing experiences and my boys keep asking me why I keep giving the truck back. They regularly have so much fun on these trips it would seem they’re sold on having a truck/rig like this of their very own.   Best of Family Taken on our trip to see the total solar eclipse, the boys enjoy hanging out in the tent atop our PacificOverlander SUV. This photo sits atop both my home and work desk. It’s probably my favorite photo of the year as it captures the raw joy my boys have on our camping excursions. I spoiled my kids with ice cream atop the Glacier Point lookout in Yosemite National Park. A moment I enjoy remembering through this quick snapshot. Wrangling family for a group photos is… a challenge. This is especially true when the boys enjoy making faces and test the patience of my wife. Every so often though a photo comes out just right. This was one that I took while we enjoyed a sunny day at a beach near Half Moon Bay. Not a technically […]

2018 has been quite the year. You wouldn’t know it from my blog, but it’s been one of the most hectic years I’ve had in a long time. Much of that centered around establishing a new home base for my family, albeit in the same area. Uprooting a family of 4 in the SF bay area is not for the light hearted. Luckily everyone has been quite excited about our move, but it has taken up most of my free time. I’m happy to say that I’m starting to get my nose above water and eager to do a bit more photography. Another development this year was my acquisition of the domain calphoto.com and the CALPHOTO California photographic condition mailing list associated with the domain. Interested in real-time photo conditions across California? Then be sure to join. CALPHOTO is comprised of a great community of people who share a common interest in photographing and preserving the beauty of our state. With limited time this year I focused on trips for my kids versus dedicated photo trips. In years past I’ve mentioned how my focus was to foster a deep appreciation of nature and the outdoors with my boys and that has firmly taken hold. I am regularly asked when our next camping trip will be and what other cool places we can visit. I also get a great deal of joy from the kids when we’re driving around town and an epiphany hits that we haven’t been to Yosemite in a while. I suppose that adds to the prospect of more photography and photo trips as they’ve been picking up an interest in taking photos too. That should give you an idea of 2018 in a nutshell. Here are my 8 best photos of 2018… Landscapes Fall Color Abstract Redwood Circle of Life Fresh Snow in Yosemite Valley Rays of Sun in the Redwoods National Forest Family Fun Someone has picked up good tripod technique by watching his dad. He started doing this on his own randomly during our trip. He was insistent he use my tripod even if he was just resting his camera on top of it without a base plate. Fun at the Trees of Mystery Trees of Mystery Portrait Holiday Photo Hijinks Thanks for looking and have a great new year!

Thanks for checking in to see my best photos of 2019. This year for a variety of reasons I didn’t have many opportunities to get out and pursue photography to the degree I’d have preferred. Some of the reasons why were good (new house, settling in, kid activities with both boys being in elementary school for the first time, etc.) and some were downright bummers (passing of my grandfather, getting used to this aging thing, etc.). Through it all though I got the kids out on some bigger trips this year beyond our regular trips to Yosemite. We drove from California to Yellowstone National Park in Wyoming and Glacier National Park in Montana. With my focus on the boys having a great experience my normal serious photographic endeavors were kept on the sideline. As always I felt good with that decision after hearing how much they loved Glacier National Park, especially given Glacier it is a personal favorite of mine. In 2020 I’m looking forward to exploring more of my favorite locations with them and seeing it all a new through their eyes.  All that being said enjoy my best of the year…   Landscapes & Wildlife Much of my time outdoors this year was spent showing the boys more of my favorite locations and experiencing the great outdoors while indulging them with great views and of course many evenings of campfires and s’mores. The following view at Two Medicine lake in Glacier National Park was incredibly memorable as we explored the shore and skipped rocks.   Of all my time at Glacier National Park I’ve never once seen a Pika… until this year. Not only are they well camouflaged and hard to see, but they’re incredibly fast. Being small even photographing them with a 400mm lens isn’t nearly enough magnification. The first photo below is a severely cropped photo taken at 400mm. The uncropped photos, 2 down, is included to give you an idea of how hard it is to find them in a rock pile. Add in their constant state of running, to forage food for the winter, you have an even tougher subject to photograph. That said I do have a good number of Pika butt photos now.   While in Glacier National Park it’s always fun to see marmots scurrying about. On a hike at Logan Pass my boys and I saw several. One of the more interesting was perched in a tree surveying his territory.   Adventures with the Kids My boys enjoying a brief encounter with a curious ground squirrel was amusing. Even more amusing was the play structure my youngest son built for him with rocks near his den.   My youngest still has not shaken his interest in photography. While he wasn’t as steadfast about using a tripod this year he still seemed to enjoy taking some ‘serious’ photos.   A brief moment my kids weren’t a blur from running around, tackling each other, and/or tackling me.   A personal favorite of mine taken by a passing hiker. Thanks unknown hiker, I salute you and your ability to capture a well framed photo while maintaining focus. You’d be surprised how hard it is to get that even with an iPhone. Thanks for checking in and Happy New Year!

In cite{CC} the authors, investigating a model of population dynamics, find the following result. Let $Omegasubset mathbb{R}^N$, $Ngeq 1$, be a bounded smooth domain. The weighted eigenvalue problem $-Delta u =lambda m u $ in $Omega$ under homogeneous Dirichlet boundary conditions, where $lambda in mathbb{R}$ and $min L^infty(Omega)$, is considered. The authors prove the existence of minimizers $check m$ of the principal positive eigenvalue $lambda_1(m)$ when $m$ varies in a class $mathcal{M}$ of functions where average, maximum, and minimum values are given. A similar result is obtained in cite{CCP} when $m$ is in the class $mathcal{G}(m_0)$ of rearrangements of a fixed $m_0in L^infty(Omega)$. In our work we establish that, if $Omega$ is Steiner symmetric, then every minimizer in cite{CC,CCP} inherits the same kind of symmetry.

We show that any toric K"ahler cone with smooth compact cross-section admits a family of Calabi-Yau cone metrics with conical singularities along its toric divisors. The family is parametrized by the Reeb cone and the angles are given explicitly in terms of the Reeb vector field. The result is optimal, in the sense that any toric Calabi-Yau cone metric with conical singularities along the toric divisor (and smooth elsewhere) belongs to this family. We also provide examples and interpret our results in terms of Sasaki-Einstein metrics.

We construct semiglobal singular spacetimes for the Einstein equations coupled to a massless scalar field. Consistent with the heuristic analysis of Belinskii, Khalatnikov, Lifshitz or BKL for this system, there are no oscillations due to the scalar field. (This is much simpler than the oscillatory BKL heuristics for the Einstein vacuum equations.) Prior results are due to Andersson and Rendall in the real analytic case, and Rodnianski and Speck in the smooth near-spatially-flat-FLRW case. Similar to Andersson and Rendall we give asymptotic data at the singularity, which we refer to as final data, but our construction is not limited to real analytic solutions. This paper is a test application of tools (a graded Lie algebra formulation of the Einstein equations and a filtration) intended for the more subtle vacuum case. We use homological algebra tools to construct a formal series solution, then symmetric hyperbolic energy estimates to construct a true solution well-approximated by truncations of the formal one. We conjecture that the image of the map from final data to initial data is an open set of anisotropic initial data.

In this paper, we give a criterion of the Gorenstein property of the Ehrhart ring of the stable set polytope of an h-perfect graph: the Ehrhart ring of the stable set polytope of an h-perfect graph $G$ is Gorenstein if and only if (1) sizes of maximal cliques are constant (say $n$) and (2) (a) $n=1$, (b) $n=2$ and there is no odd cycle without chord and length at least 7 or (c) $ngeq 3$ and there is no odd cycle without chord and length at least 5.

Bernstein polynomials, long a staple of approximation theory and computational geometry, have also increasingly become of interest in finite element methods. Many fundamental problems in interpolation and approximation give rise to interesting linear algebra questions. When attempting to find a polynomial approximation of boundary or initial data, one encounters the Bernstein-Vandermonde matrix, which is found to be highly ill-conditioned. Previously, we used the relationship between monomial Bezout matrices and the inverse of Hankel matrices to obtain a decomposition of the inverse of the Bernstein mass matrix in terms of Hankel, Toeplitz, and diagonal matrices. In this paper, we use properties of the Bernstein-Bezout matrix to factor the inverse of the Bernstein-Vandermonde matrix into a difference of products of Hankel, Toeplitz, and diagonal matrices. We also use a nonstandard matrix norm to study the conditioning of the Bernstein-Vandermonde matrix, showing that the conditioning in this case is better than in the standard 2-norm. Additionally, we use properties of multivariate Bernstein polynomials to derive a block $LU$ decomposition of the Bernstein-Vandermonde matrix corresponding to equispaced nodes on the $d$-simplex.

The invention relates compounds of general formula (I) and pharmaceutically acceptable salts and solvates thereof wherein R1 is halogen, vinylene-aryl, substituted aryl, heteroaryl or a benzo[1,3]dioxolil group,W is a group of formula —NH—SO2—R2 or heteroaryl group or NHR3 group where R3 is hydrogen or heteroaryl; and n is 1, 2, 3 or 4. Furthermore, the present invention is directed to pharmaceutical composition containing at least one compound of general formula (I) and/or pharmaceutically acceptable salts or solvates thereof and for the use of them for the preparation of pharmaceutical compositions for the prophylaxis and/or the treatment of protein kinase related, especially CDK9-related diseases e.g. cell proliferative disease, infectious disease, pain, cardiovascular disease and inflammation.

RNA interference is provided for inhibition of Frizzled Related Protein-1 mRNA expression, in particular, for treating patients having glaucoma or at risk of developing glaucoma.

The present invention relates to the field of cardiology. More specifically, the present invention provides methods and compositions for inducing proliferation of cardiomyocytes. In a specific embodiment, a method for inducing proliferation of cardiomyocytes comprises the step of administering an effective amount of an ALMS1 inhibitor.

A method of labeling a sulfenic acid (—SOH) group of a cysteine residue in a protein; or peptide, comprises contacting said protein or peptide with a beta-ketoester to covalently couple said beta-ketoester to said cysteine residue and form a beta-ketoester-labeled cysteine residue in said protein or peptide.

Tissue slices and whole organisms offer substantial challenges to fluorescence imaging. Autofluorescence and absorption via intrinsic chromophores, such as flavins, melanin, and hemoglobins, confound and degrade output from all fluorescent tags. An “optical window,” farther red than most autofluorescence sources and in a region of low hemoglobin and water absorbance, lies between 650 and 900 nm. This valley of relative optical clarity is an attractive target for fluorescence-based studies within tissues, intact organs, and living organisms. Novel fluorescent tags were developed herein, based upon a genetically targeted fluorogen activating protein and cognate fluorogenic dye that yields emission with a peak at 733 nm exclusively when complexed as a “fluoromodule”. This tool improves substantially over previously described far-red/NIR fluorescent proteins in terms of brightness, wavelength, and flexibility by leveraging the flexibility of synthetic chemistry to produce novel chromophores.

The present invention relates to novel synthetic substituted heterocyclic compounds and pharmaceutical compositions containing the same that are capable of inhibiting or antagonizing a family of receptor tyrosine kinases, Tropomysosin Related Kinases (Trk), in particular the nerve growth factor (NGF) receptor, TrkA. The invention further concerns the use of such compounds in the treatment and/or prevention of pain, cancer, restenosis, atherosclerosis, psoriasis, thrombosis, or a disease, disorder or injury relating to dysmyelination or demyelination or the disease or disorder associated with abnormal activities of NGF receptor TrkA.

The invention is directed to novel substituted benzylamino quinolines, compounds comprising substituted benzylamino quinolines, methods of making substituted benzylamino quinolines, the use of substituted benzylamino quinolines for treating or preventing a variety of conditions or diseases associated with lipoprotein metabolism, and the use of substituted benzylamino quinolines as cholesterol ester-transfer protein inhibitors.

One-part binder compositions are described that may include a protein and a crosslinking combination. The crosslinking combination may include at least a first crosslinking compound and a second crosslinking compound. The first and second crosslinking compounds are individually crosslinkable with each other and with the protein. Examples of the protein include soy protein. Fiber products and methods of making the fiber products are also described. The fiber products may include organic fibers, inorganic fibers, or both, in a cured thermoset binder based on solutions of the one-part binder compositions.

A polymeric dendrimer-like structure with four branches of monomethoxy-polyethylene glycol that can be represented as: The carboxylic group of the previous structure can be functionalized for the production of conjugates of pharmaceutical interest. The binding of this dendrimer-like polyethylene glycol to therapeutic proteins improves their in vitro and in vivo stability.

A wood-containing composite are described that may include a lignocellulosic material, and a formaldehyde-free binder in contact with at least a portion of the lignocellulose material. The binder is formed from a binder composition that includes a soy flour, a polymer, and a crosslinking agent, at least a portion of each of which are covalently crosslinked to each other in the binder. Also described are methods of making wood-containing composites by providing a pre-mixed, one-part binder composition of at least 60 wt. % soy flour, a polymer, and a crosslinking agent. The binder composition is applied to lignocellulosic material, and the combination may be heated at a temperature of about 100° C. or more to cure the binder composition into a binder. The cured binder has the soy protein, polymer, and crosslinking agent covalently bonded to each other.

The invention provides protein adhesives and methods of making and using such adhesives. The protein adhesives contain ground plant meal or an isolated polypeptide composition obtained from plant biomass.

One-part binder compositions are described that may include a protein and a crosslinking combination. The crosslinking combination may include at least a first crosslinking compound and a second crosslinking compound. The first and second crosslinking compounds are individually crosslinkable with each other and with the protein. Examples of the protein include soy protein. Fiber products and methods of making the fiber products are also described. The fiber products may include organic fibers, inorganic fibers, or both, in a cured thermoset binder based on solutions of the one-part binder compositions.

The present disclosure provides a photoreactive synthetic regulator of protein function. The present disclosure further provides a light-regulated polypeptide that includes a subject synthetic regulator. Also provided are cells and membranes comprising a subject light-regulated polypeptide. The present disclosure further provides methods of modulating protein function, involving use of light.

Disclosed herein are compounds that inhibit Hsp90 interactions with IAP proteins, such as Survivin, XIAP, cIAP1, or cIAP2, and methods for identifying and using such compounds.

The present invention relates to isolation of whey proteins and the preparation of a whey product and a whey isolate. In particular the present invention relates to the isolation of a β-lactoglobulin product and the isolation of an α-enriched whey protein isolate from whey obtained from an animal. The α-enriched whey protein isolate provided by the present invention is besides from being low in β-lactoglobulin also high in α-lactalbumin and immunoglobulin G.

The present invention relates to novel muteins derived from human lipocalin 2 (hNGAL) and related proteins that bind a given non-natural ligand with detectable affinity. The invention also related to corresponding nucleic acid molecules encoding such a mutein and to a method for their generation. The invention further relates to a method for producing such a mutein. Furthermore, the invention is directed to a pharmaceutical composition comprising such a lipocalin mutein as well as to various uses of the mutein.

A method of forming a cross-linked protein structures includes preparing a solution of protein dissolved in a benign solvent and forming an intermediate protein structure from the solution. The intermediate protein structure can be cross-linked by providing for a specific ratio of chemical cross-linking agents to form the cross-linked protein structure. The solution can be prepared by adding a cross-linker of N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) at a ratio of two-to-one of NHS to EDC to alcohol. PBS buffer (20×) can be added to the solution until the volume ratio of PBS buffer (20×) to alcohol is about one-to-one. About 16 percent by weight of protein can be dissolved in the solution. The solution can be electrospun to form an intermediate protein structure. After a period of time, the protein structure can be cross-linked to form the cross-linked protein structure.

The present application relates to antigen-binding proteins that are capable of binding to mammalian IgG. The frame-work regions of the antigen-binding proteins of the application preferably correspond to those of antibodies naturally that are devoid of light chains as may e.g. be found in camelids. The application further relates to nucleic acids that encode such antigen-binding proteins, to immunoadsorbent materials that comprise such proteins, to the uses of such immunoadsorbent materials for the purification of mammalian IgG antibodies and for therapeutic apheresis.

Antigen binding proteins with T cell receptor-like paratopes, that is, with an antigen binding region specific for an human leukocyte antigen-A2 restricted peptide are disclosed. The antigen binding proteins encompass antibodies in a variety of forms, including full-length antibodies, substantially intact antibodies, Fab fragments, F(ab')2 fragments, and single chain Fv fragments. Fusion proteins, such as scFv fusions with immunoglobulin or T-cell receptor domains, incorporating the specificity of the antigen binding region for each peptide are also contemplated by the invention. Furthermore, immunoconjugates may include antibodies to which is linked a radioisotope, fluorescent or other detectable marker, cytotoxin, or other molecule are also encompassed by the invention. Among other things, immunoconjugates can be used for delivery of an agent to elicit a therapeutic effect or to facilitate an immune effector function.

A chimeric protein containing neutrophil inhibitory factor and hirugen, the chimeric protein having an amino acid sequence that includes FPRPGSGG (SEQ ID NO:21) Also provided is a pharmaceutical composition comprising the chimeric protein, which can be used for treating or preventing cerebral injury and cerebral edema, or for inhibiting platelet aggregation.

A protein which is composed N-terminally of one or several C-terminal parts of the amino acid sequence of the mature tumor necrosis factor and C-terminally of one or several N-terminal parts of the amino acid sequence of the mature tumor necrosis factor, which activates epithelial ion channels and improves the lung function and which can be used for the manufacture of medicaments for the treatment of diseases associated with the lung function, such as oedemas.

The present invention provides a method for enhancing an immune response in a mammal to facilitate the elimination of a chronic pathology. The method involves the removal of immune system inhibitors such as soluble TNF receptor from the circulation of the mammal, thus, enabling a more vigorous immune response to the pathogenic agent. The removal of immune system inhibitors is accomplished by contacting biological fluids of a mammal with one or more binding partner(s) such as TNFα muteins capable of binding to and, thus, depleting the targeted immune system inhibitor(s) from the biological fluids. Particularly useful in the invention is an absorbent matrix composed of an inert, biocompatible substrate joined covalently to a binding partner, such as a TNFα mutein, capable of specifically binding to a targeted immune system inhibitor such as soluble TNF receptor.

Compositions and methods for the treatment of asthma and inflammatory ocular disorders are disclosed.

Disclosed herein are polypeptides, polynucleotides encoding, cells and organisms comprising novel DNA-binding domains, including TALE DNA-binding domains. Also disclosed are methods of using these novel DNA-binding domains for modulation of gene expression and/or genomic editing of endogenous cellular sequences.

An insect-resistant protein exhibiting a sufficient resistance to insects; an insect-resistance gene encoding the insect-resistant protein; a recombinant vector containing the insect-resistance gene; a host cell and a plant cell having the recombinant vector transfected thereinto; a transformant transformed by the insect-resistance gene and a method for producing the same; a protein recovered thereby; and an insect-resistant agent comprising them as active ingredients. In accordance with the present invention, the insect-resistant protein is derived from a plant.

Disclosed are polypeptides, nucleic acids, and related compositions that render plants resistant to bacterial pathogens. Also disclosed are transgenic plants having the nucleic acids and resistant to bacterial pathogens.

IL4/IL13-binding proteins comprise binding domains, which inhibit IL4/IL13 binding to IL4Ralpaha and common gamma chain complexes (Type 1) and inhibit IL4 binding to IL4Ralpha and IL13Ralpha1 complexes (Type 2), and IL13 binding to IL13Ralpha1 and/or IL13Ralpha2, are useful in the treatment of cancer, inflammatory, and other pathological conditions, such as allergic or fibrotic conditions, especially pulmonary conditions.

The present invention provides variable mass labeling reagents, a set of the variable mass labeling reagents, and a multiplexed set of variable mass labeling reagents.

Microfluidic devices having a protein renaturation component and methods for using the same are provided. Aspects of the present disclosure include microfluidic devices that include a separation medium with a first flow path and a protein renaturation component in fluid communication with the separation medium and having a second flow path. Also provided are methods of using the devices as well as systems and kits that include the devices. The devices, systems and methods find use in a variety of different applications, including diagnostic and validation assays.

The invention encompasses analyzers and analyzer systems that include a single particle analyzer, methods of using the analyzers and analyzers systems to analyze samples, either for single particles, e.g., protein molecules, or for multiple particles (multiplexing), methods of doing business based on the use of the analyzers or analyzer systems of the system, and electronic media for storing parameters useful in the analyzers and analyzer systems of the invention.

A process for cooking a food in oil and/or fat is provided. A dry pea protein mixture or an aqueous pea protein is added to a food prior to cooking. The amount of oil and/or fat absorbed by the food during cooking is substantially reduced.

The present invention provides protein hydrolysate compositions, processes for making protein hydrolysate compositions, and food products comprising protein hydrolysate compositions. The protein hydrolysate compositions generally comprise polypeptide fragments having primarily either an arginine residue or a lysine residue at each carboxyl terminus.

Isolated wheat proteins that are substantially free of sulfites and compositions formed from the isolated wheat proteins are described. Methods of forming compositions which include isolated wheat proteins that are substantially free of sulfites are also described.