System Design for Advanced Beginners — a friendly explanation of the what and why of systems, with acknowledgement of the real world like There are many tools out there, each with different strengths and weaknesses, and many ways to build a technology company. The real, honest reasons that we will make many of our technological […]

The Fuzzy Edges of Character Encoding — the history, politics, and computational basics of text-based character encoding and digital representations of text, from Morse Code to ASCII to Unicode (and emoji), as well as alternative text encoding schemes. (via Everest Pipkin) AutoHotkey — an automation scripting language for Windows. The Electronic Nose and its Applications: […]

FairMOT — one-shot multi-object tracking that remarkably outperforms the state-of-the-arts on the MOT challenge datasets at 30 FPS. pipedream — IFTTT for coders. Compiler Explorer — an interactive tool that lets you type code in one window and see the results of its compilation in another window. Using the site should be pretty self-explanatory: by […]

Introduction to COBOL — a 1999 web site (!) with slides from a University of Limerick course. IBM will offer free (presumably more modern) training. zoombot — a highly advanced AI to handle Zoom calls. storybook.js — open source toolkit and sandbox to build UI components in isolation so you can develop hard-to-reach states and […]

The Science of Happiness — free enrolment in Berkeley’s MOOC to teach positive psychology. Learn science-based principles and practices for a happy, meaningful life. The New Business of AI (A16Z) — many AI companies have: Lower gross margins due to heavy cloud infrastructure usage and ongoing human support; Scaling challenges due to the thorny problem […]

Coding vs Programming (John Gruber) — I’d noticed this linguistic change too. See also Engineering vs Programming vs Computer Science. Coding is shorter so it’s probably gaining in popularity because shorter is easier to say and thus more convenient. micrograd (Andre Karpathy) — A tiny Autograd engine (with a bite! :D). Implements backpropagation (reverse-mode autodiff) […]

Kanboard — free and open source Trello-like Kanban boards. Remote Work Playbook — really useful advice on the actual mechanics of working remotely, not just which tools to use but how to use them. E.g., As an individual contributor, is there something you just did that you think a colleague would have to do at […]

Nebula —open source distributed, scalable, lightning-fast graph database. COBOL Programming Course — from the Open Mainframe Project. Serverless Handbook — a resource teaching frontend engineers everything they need to know to dive into backend. Novel Annealing Processor Is the Best Ever at Solving Combinatorial Optimization Problems (IEEE Spectrum) — Dubbed STATICA (Stochastic Cellular Automata Annealer […]

CastleDB — a structured static database […]. CastleDB looks like any spreadsheet editor, except that each sheet has a data model. […] stores both its data model and the data contained in the rows into an easily readable JSON file. […] allows efficient collaboration on data editing. Mainframes Are Having a Moment (IEEE Spectrum) — […]

It’s Time to Learn (Scott Berkun) — a strong response to Marc Andreessen’s It’s Time to Build. It feels like we are in a disrupted time when anything is possible, and folks are wondering where the levers are to pull. pygraphistry — a library to extract, transform, and visually explore big graphs. Desert Island Devops […]

Posthog — open source product analytics. Into the Mainframe (Recurse) — the interviews with two mainframe programmers are a great reminder of how much things have changed. And how they haven’t. For instance, later in my career I kept a weighted punching clown in my office. As programmers, we liked our users, but we also […]

Moloch — Large scale, open source, indexed packet capture and search. 3Dify Instagram Photos — open source toolset for adding a 3d effect to photos on Instagram’s web site. It uses 3d-photo-inpainting running in Colab (free GPU) and Cloud pubsub/storage for communication. A glimpse of the future: we could augment all our apps with deep […]

The Suddenly Remote Playbook — I just want to note that if you have to look after kids when you’re supposed to be working, you’re not working from home. Not everyone’s getting a glorious introduction to the delights of working from home. taichi — a programming language designed for high-performance computer graphics. It is deeply […]

Teleforking a Process onto a Different Computer — a working proof of concept (I just don’t replicate tricky things so that I could keep it simple, meaning it’s just a fun tech demo you probably shouldn’t use for anything real) of a telefork() function call that spawns a process on another machine and returns the […]

Learning a Language — this list of questions facing anyone taking a new language for a test run just burns with truth. (Also: encouraging to see how many of these questions are answered by the Cookbook format) OpenVAS — Open Vulnerability Assessment Scanner, aka “what a cheap external security assessment vendor will run and then […]

podpaperscissors — From the classic “prisoner’s dilemma” to more obscure coördination games, Pod Paper Scissors takes game theory out of the dry textbook and into the real world. … Each episode will feature different kinds of games and situations. Experts in a variety of fields will casually converse with the hosts about how the particular […]

To Microservices and Back Again: Why Segment Went Back to a Monolith — microservices came with increased operational overhead and problems around code reuse. … If microservices are implemented incorrectly or used as a band-aid without addressing some of the root flaws in your system, you’ll be unable to do new product development because you’re […]

Tasmota — Alternative firmware for ESP8266 with easy configuration using webUI, OTA updates, automation using timers or rules, expandability and entirely local control over MQTT, HTTP, Serial or KNX. Selfie 2 Waifu — deep learning constructs an anime character from your photo. Paper for the underlying technique. (via @tkasasagi) The Handbook of Cyber Wargames: Wargaming […]

Popcorn Linux — exploring how to improve the programmability of emerging heterogeneous hardware, in particular, those with Instruction Set Architecture (ISA)-diverse cores, from node-scale (e.g., Xeon/Xeon-Phi, ARM/x86, CPU/GPU/FPGAs) to rack-scale (e.g., Scale-out processors, Firebox, The Machine), in both native and virtualized settings. Additionally, the project is exploring how to automatically compile/synthesize/execute code on ISA-heterogeneous hardware. […]

Leaving Amazon (Tim Bray) — May 1st was my last day as a VP and Distinguished Engineer at Amazon Web Services, after five years and five months of rewarding fun. I quit in dismay at Amazon firing whistleblowers who were making noise about warehouse employees frightened of Covid-19. Observability is a Many-Splendoured Thing (Charity Majors) […]

Raman Spectroscopy — Low Cost, High Performances, 100% Open Source Raman Spectrometer. […] We currently offer the spectrometer in a Starter Edition version designed for teaching Raman spectroscopy and we will soon release a Performance Edition version which achieves a tested 12 cm-1 resolution at low costs. Great to see this getting into the hands […]

Super Bootable 64 — Super Mario 64 shipped before the SDK was finalised, and it had to be compiled with optimisations turned off. This meant the binary was easily reversed to source code, and now the unportable has been ported. This site probably won’t last long, because DMCA, but it’s technically a sweet feat. (via […]

Mathematics for Machine Learning — We wrote a book on Mathematics for Machine Learning that motivates people to learn mathematical concepts. The book is not intended to cover advanced machine learning techniques because there are already plenty of books doing this. Instead, we aim to provide the necessary mathematical skills to read those other books. […]

Who is Patrick Mason? I just ran across a few short horror films he wrote and directed, and they're truly scary. Like edge-of-your-seat gasp-out-loud scary. Not only that, but they're beautifully made with good actors, especially Ayuda (see below). The three videos posted here are the ones I've seen so far, but there are more on his site, which I plan to watch tonight. Can't wait to see where this director takes us next.

Read the rest

You’ve got a short story that goes on too long? A chapter that reads at a dying snail’s pace? A challenge entry that trails beyond the maximum word count? You’re also relatively new to writing and haven’t yet dragged your way through all the millions of writing articles? If yes to the last and anything else to the rest, here’s your article.

1. Remove all redundancies. I repeat: Remove all redundancies. I repeat: ….

“Unknown strangers” or “asked a question” can be removed right away. While it may sound silly, there’s a good chance you have a few of those rummaging around in your work for you to weed out.

More significantly you will want to root out the various strings of wordy words writers conjure up. “At that point in time,” can be replaced with “back then,” or even “then,” if the placement in the sentence allows it. “Despite the fact” can be “although.” “Less than great,” is just a polite man’s “mediocre.”

As a lone appreciator of purple prose I won’t tell you to always cull all of these combos, for there’s a time and place for everything and it’s called colle… a long-form story, but if you’re trying to shorten things up these are the first words to go.

Continue reading Keeping It Short at Mythic Scribes.

Gijs den Besten
Sep 1, 2013; 54:2325-2340
Reviews

Optic atrophy 1 (OPA1) is a dynamin protein that mediates mitochondrial fusion at the inner membrane. OPA1 is also necessary for maintaining the cristae and thus essential for supporting cellular energetics. OPA1 exists as membrane-anchored long form (L-OPA1) and short form (S-OPA1) that lacks the transmembrane region and is generated by cleavage of L-OPA1. Mitochondrial dysfunction and cellular stresses activate the inner membrane–associated zinc metallopeptidase OMA1 that cleaves L-OPA1, causing S-OPA1 accumulation. The prevailing notion has been that L-OPA1 is the functional form, whereas S-OPA1 is an inactive cleavage product in mammals, and that stress-induced OPA1 cleavage causes mitochondrial fragmentation and sensitizes cells to death. However, S-OPA1 contains all functional domains of dynamin proteins, suggesting that it has a physiological role. Indeed, we recently demonstrated that S-OPA1 can maintain cristae and energetics through its GTPase activity, despite lacking fusion activity. Here, applying oxidant insult that induces OPA1 cleavage, we show that cells unable to generate S-OPA1 are more sensitive to this stress under obligatory respiratory conditions, leading to necrotic death. These findings indicate that L-OPA1 and S-OPA1 differ in maintaining mitochondrial function. Mechanistically, we found that cells that exclusively express L-OPA1 generate more superoxide and are more sensitive to Ca2+-induced mitochondrial permeability transition, suggesting that S-OPA1, and not L-OPA1, protects against cellular stress. Importantly, silencing of OMA1 expression increased oxidant-induced cell death, indicating that stress-induced OPA1 cleavage supports cell survival. Our findings suggest that S-OPA1 generation by OPA1 cleavage is a survival mechanism in stressed cells.

Dorte B. Bekker-Jensen
Apr 1, 2020; 19:716-729
Technological Innovation and Resources

State-of-the-art proteomics-grade mass spectrometers can measure peptide precursors and their fragments with ppm mass accuracy at sequencing speeds of tens of peptides per second with attomolar sensitivity. Here we describe a compact and robust quadrupole-orbitrap mass spectrometer equipped with a front-end High Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS) Interface. The performance of the Orbitrap Exploris 480 mass spectrometer is evaluated in data-dependent acquisition (DDA) and data-independent acquisition (DIA) modes in combination with FAIMS. We demonstrate that different compensation voltages (CVs) for FAIMS are optimal for DDA and DIA, respectively. Combining DIA with FAIMS using single CVs, the instrument surpasses 2500 peptides identified per minute. This enables quantification of >5000 proteins with short online LC gradients delivered by the Evosep One LC system allowing acquisition of 60 samples per day. The raw sensitivity of the instrument is evaluated by analyzing 5 ng of a HeLa digest from which >1000 proteins were reproducibly identified with 5 min LC gradients using DIA-FAIMS. To demonstrate the versatility of the instrument, we recorded an organ-wide map of proteome expression across 12 rat tissues quantified by tandem mass tags and label-free quantification using DIA with FAIMS to a depth of >10,000 proteins.

1 June 2008 , Number 6

Dmitri Medvedev’s appearance at the G8 Summit in Hokkaido will be his first step on the wide international stage that Vladimir Putin occupied with such a swagger. Expectations of change will be high, but they are likely to be unrealistic at such an early stage in Medvedev’s presidency. Putin’s foreign policy legacy is a heavy one and he has made it clear that Medvedev will be no soft touch, but will his approach ultimately be more productive?

Optic atrophy 1 (OPA1) is a dynamin protein that mediates mitochondrial fusion at the inner membrane. OPA1 is also necessary for maintaining the cristae and thus essential for supporting cellular energetics. OPA1 exists as membrane-anchored long form (L-OPA1) and short form (S-OPA1) that lacks the transmembrane region and is generated by cleavage of L-OPA1. Mitochondrial dysfunction and cellular stresses activate the inner membrane–associated zinc metallopeptidase OMA1 that cleaves L-OPA1, causing S-OPA1 accumulation. The prevailing notion has been that L-OPA1 is the functional form, whereas S-OPA1 is an inactive cleavage product in mammals, and that stress-induced OPA1 cleavage causes mitochondrial fragmentation and sensitizes cells to death. However, S-OPA1 contains all functional domains of dynamin proteins, suggesting that it has a physiological role. Indeed, we recently demonstrated that S-OPA1 can maintain cristae and energetics through its GTPase activity, despite lacking fusion activity. Here, applying oxidant insult that induces OPA1 cleavage, we show that cells unable to generate S-OPA1 are more sensitive to this stress under obligatory respiratory conditions, leading to necrotic death. These findings indicate that L-OPA1 and S-OPA1 differ in maintaining mitochondrial function. Mechanistically, we found that cells that exclusively express L-OPA1 generate more superoxide and are more sensitive to Ca2+-induced mitochondrial permeability transition, suggesting that S-OPA1, and not L-OPA1, protects against cellular stress. Importantly, silencing of OMA1 expression increased oxidant-induced cell death, indicating that stress-induced OPA1 cleavage supports cell survival. Our findings suggest that S-OPA1 generation by OPA1 cleavage is a survival mechanism in stressed cells.

There is a three-minute video about MathSciNet now available online on Vimeo. It is also available as part of a blog post from EBSCO, which mostly discusses Mathematics and Statistics Awareness Month and the really neat book Living Proof: Stories … Continue reading →

This week we saw the release of the much awaited Yellowhammer documents from the government, documents which outline some of the risks involved with Britain’s sudden departure from the EU. The documents themselves outline that there are risks to the supply of medicines - but do not set out the detail of how those risks have been mitigated, and...

WITH THE productive sector experiencing a shortage of foreign exchange for the past two months, the Jamaican Manufacturers and Exporters Association, JMEA, says it has appealed to the Bank of Jamaica, BOJ, for direct access to US currency for three...

China has been Africa’s largest trading partner since 2009, and as commerce and investment have increased, so have flows of people in both directions. With an estimated 1 million to 2 million Chinese migrants across Africa, some countries have relaxed their short-term visa requirements in hopes of facilitating cultural and business exchanges. High levels of Chinese investment do not, however, correlate with more liberal visa policies, as this article explores.

Association staff and members of the ADA Advisory Task Force on Dental Practice Recovery are aware and working diligently in addressing members’ concerns over the limited availability of certain personal protective equipment items.

An unexpected rise in U.S. crude inventories offset fears of potential risk to Venezuelan crude supply because of possible U.S. sanctions.

A 29 years old female presented to us in the metabolic clinic of the University of Port Harcourt Teaching Hospital (UPTH) on account of a week history of easy fatigability, weakness, and lower extremity muscle cramps associated with numbness and tingling sensation in the peri-oral area, fingers and toes. Two weeks prior to the onset of her presenting symptoms, she had visited a local pharmaceutical shop on account of a distressing epigastric discomfort and was subsequently placed on daily oral omeprazole 20mg daily for a month by a pharmacist. She had been on the omeprazole medication for two weeks before her present symptoms manifested. Her past medical history was not suggestive of hypoparathyroidism nor pancreatitis. She was married with three children and has an uneventful family, social and obstetric histories. On examination, she was a healthy well-oriented young female with positive Trousseau’s, Chvostek’s and epigastric tenderness signs. Further Laboratory evaluation revealed she had low plasma magnesium, low plasma albumin-corrected calcium, and low serum parathyroid hormone levels, while other laboratory parameters were essentially normal. A diagnosis of omeprazole-induced electrolyte disorders (hypomagnesaemia and hypocalcaemia) associated with hypoparathyroidism was made following the review of her clinical examination and laboratory findings. She was subsequently managed with oral magnesium supplements following the withdrawal of the omeprazole medication (replaced with oral ranitidine), monitored weekly, and full recovery was achieved after three weeks.

India is the world's largest source for immigrant physicians, and for Indian-trained doctors and nurses the allure of working abroad is strong despite an acute domestic shortage of health-care workers. Against this pull, the Indian government has enacted a number of policies to limit and regulate the emigration of health-care professionals, though these have been more ad hoc in nature and not part of a fully realized strategy.

A modern flavour twist on an old favourite. Enjoy!

Delicious steak with chimmi churri sauce