1. ICMR partners with Bharat Biotech International Limited for developing indigenous coronavirus COVID-19 vacc...  Zee News
2. Bharat Biotech to lead monoclonal antibodies project for Covid-19 therapy  The Financial Express
3. Bharat Biotech to lead project on monoclonal antibodies therapy for COVID-19  The New Indian Express
4. ICMR teams up with Bharat Biotech to develop Covid-19 vaccine  Times of India
5. CSIR approves project to develop human monoclonal antibodies that can neutralize COVID-19 in patients  Times Now
6. View Full coverage on Google News

ICMR teams up with Bharat Biotech to develop COVID-19 vaccine  The Financial ExpressView Full coverage on Google News

Protego Diabolica is the spell Grindelwald used to make those flames. What does that mean?

What "answers will be given"?

What is Mr. Blood? We...kind of can't remember

FB going to Brazil

Globus Mundi appears in the wizarding world

More about Leta Lestrange: what did Grindelwald really say about her? Is she really gone?

Is Newt on the spectrum of autism?

The big magical fights in the books, vs those in the movies

The return of the Mailbag!

...and more!

There’s nothing stopping Jimmy Donaldson aka Mr. Beast from giving away money, this time doing it via video calls. Watch as he and his friends pretend to be a news reporter, school professor, and many others as they interview people, and then surprise them by giving them thousands of dollars. Some recognize him and his team, however.

“A lot of people are going through a ruff time right now so I tried my best to do some good,” he said in the video description. He also posted this on his birthday, and said that liking or subscribing would be “a dope present.”

Very wholesome.

(Video Credit: MrBeast/ YouTube)

Pillow pug!

zmrd o to zákeřnější, že vždy útočí ze zálohy Příklad: „Vladěno, tamten chlápek je pořádný přizdizmrd, bacha na něho!”

Příspěvek přizdizmrd pochází z Čeština 2.0

The author of “The New York Nobody Knows: Walking 6,000 Miles in the City,” was 74.

Trump is correct that the decision is a very tough call. But it will be made by mayors and governors, not federal officials.

The Angels shut down Griffin Canning last summer because of elbow soreness. After his first appearance this spring, the soreness has returned.

The Angels announced starting pitcher Griffin Canning's MRI revealed "chronic changes to the UCL and acute joint irritation" in his right elbow.

"Mrs. America" creator Dahvi Waller on the books to read if you want to know more about the ERA

Helmreich, 74, an academic with eclectic interests whose areas of expertise ranged from race relations to urban life to Orthodox Jewry.

What the 'Mrs. America' gets right and wrong about Brenda and Marc Feigen Fasteau and Phyllis Schlafly's son in Episode 5.

"Mrs. America" depicts the battle over the Equal Rights Amendment in the 1970s. We're fact-checking its historical accuracy, episode by episode.

MRS HINCH - also known as Sophie Hinchcliffe - is an Instagram cleaning sensation who has inspired millions to clean their home. She is known for her cheap cleaning hacks and quick easy methods. A Hinch fan has recently revealed how she got her windows gleaming with minimal effort.

MRS HINCH - aka Sophie Hinchcliffe - is an Instagram cleaning sensation who has inspired millions over the past year to clean their homes. She reveals cheap and quick cleaning hacks online to help people speed clean their homes. A fan has recently revealed a quick hack on how to remove hair and dust from carpets with easy to follow steps.

Scooby Johnson of Benton Harbor is the 40th Michigan Mr. Basketball, narrowly beating out Flint Beecher's Jalen Terry for the award.

Avon grad among four local transfers trying to become starting QBs

Anthony Leal put individual numbers aside at Bloomington South. The result was a 26-0 record.

ICMR teams up with Bharat Biotech to develop Covid-19 vaccine  Times of IndiaView Full coverage on Google News

MRG is excited to release its highly comprehensive annual study IPTV Market Leader Report by Product and Region — Fall 2012.

MRG’s IPTV Market Leader Reports identify which IPTV systems and software companies are leading each of the six IPTV market segments: access systems, video headend systems, video-on-demand server software, set-top boxes, middleware, and content protection/digital rights management. The data is further segmented by geographic region as follows: North America, Latin America/Caribbean, Western Europe, Eastern Europe, Asia Pacific and the Middle East/Africa.

“IPTV is continuing its rapid growth worldwide but some of the more exciting opportunities are only just beginning in the Middle East/Africa and Latin America/Caribbean regions,” said Jose Alvear, Senior Research Analyst at MRG. “While the growth in the number of new IPTV service providers has been slowing down because telcos around the world have already deployed IPTV services for a number of years, there are still plenty of new opportunities available to vendors in emerging markets. In addition, vendors are now supplying multi-screen, content delivery and OTT services, as the global pay-TV industry continues to evolve into new screens and devices.”

strataconf: RT @bostontweetup: WEDS 6PM #BostonHealth7 hosted by @bostonpainpoint @mrkrieger @health_box @strataconf http://t.co/U6tj1uLR2Q @kalyankalwa

The transcription factor forkhead box P3 (FOXP3) is a biomarker for regulatory T cells and can also be expressed in cancer cells, but its function in cancer appears to be divergent. The role of hepatocyte-expressed FOXP3 in hepatocellular carcinoma (HCC) is unknown. Here, we collected tumor samples and clinical information from 115 HCC patients and used five human cancer cell lines. We examined FOXP3 mRNA sequences for mutations, used a luciferase assay to assess promoter activities of FOXP3's target genes, and employed mouse tumor models to confirm in vitro results. We detected mutations in the FKH domain of FOXP3 mRNAs in 33% of the HCC tumor tissues, but in none of the adjacent nontumor tissues. None of the mutations occurred at high frequency, indicating that they occurred randomly. Notably, the mutations were not detected in the corresponding regions of FOXP3 genomic DNA, and many of them resulted in amino acid substitutions in the FKH region, altering FOXP3's subcellular localization. FOXP3 delocalization from the nucleus to the cytoplasm caused loss of transcriptional regulation of its target genes, inactivated its tumor-inhibitory capability, and changed cellular responses to histone deacetylase (HDAC) inhibitors. More complex FKH mutations appeared to be associated with worse prognosis in HCC patients. We conclude that mutations in the FKH domain of FOXP3 mRNA frequently occur in HCC and that these mutations are caused by errors in transcription and are not derived from genomic DNA mutations. Our results suggest that transcriptional mutagenesis of FOXP3 plays a role in HCC.

Heat shock factor 1 (HSF1) regulates cellular adaptation to challenges such as heat shock and oxidative and proteotoxic stresses. We have recently reported a previously unappreciated role for HSF1 in the regulation of energy metabolism in fat tissues; however, whether HSF1 is differentially expressed in adipose depots and how its levels are regulated in fat tissues remain unclear. Here, we show that HSF1 levels are higher in brown and subcutaneous fat tissues than in those in the visceral depot and that HSF1 is more abundant in differentiated, thermogenic adipocytes. Gene expression experiments indicated that HSF1 is transcriptionally regulated in fat by agents that modulate cAMP levels, by cold exposure, and by pharmacological stimulation of β-adrenergic signaling. An in silico promoter analysis helped identify a putative response element for activating transcription factor 3 (ATF3) at −258 to −250 base pairs from the HSF1 transcriptional start site, and electrophoretic mobility shift and ChIP assays confirmed ATF3 binding to this sequence. Furthermore, functional assays disclosed that ATF3 is necessary and sufficient for HSF1 regulation. Detailed gene expression analysis revealed that ATF3 is one of the most highly induced ATFs in thermogenic tissues of mice exposed to cold temperatures or treated with the β-adrenergic receptor agonist CL316,243 and that its expression is induced by modulators of cAMP levels in isolated adipocytes. To the best of our knowledge, our results show for the first time that HSF1 is transcriptionally controlled by ATF3 in response to classic stimuli that promote heat generation in thermogenic tissues.

Guoan Chen
Apr 1, 2002; 1:304-313
Research

Although a robust inflammatory response is needed to combat infection, this response must ultimately be terminated to prevent chronic inflammation. One mechanism that terminates inflammatory signaling is the production of alternative mRNA splice forms in the Toll-like receptor (TLR) signaling pathway. Whereas most genes in the TLR pathway encode positive mediators of inflammatory signaling, several, including that encoding the MyD88 signaling adaptor, also produce alternative spliced mRNA isoforms that encode dominant-negative inhibitors of the response. Production of these negatively acting alternatively spliced isoforms is induced by stimulation with the TLR4 agonist lipopolysaccharide (LPS); thus, this alternative pre-mRNA splicing represents a negative feedback loop that terminates TLR signaling and prevents chronic inflammation. In the current study, we investigated the mechanisms regulating the LPS-induced alternative pre-mRNA splicing of the MyD88 transcript in murine macrophages. We found that 1) the induction of the alternatively spliced MyD88 form is due to alternative pre-mRNA splicing and not caused by another RNA regulatory mechanism, 2) MyD88 splicing is regulated by both the MyD88- and TRIF-dependent arms of the TLR signaling pathway, 3) MyD88 splicing is regulated by the NF-κB transcription factor, and 4) NF-κB likely regulates MyD88 alternative pre-mRNA splicing per se rather than regulating splicing indirectly by altering MyD88 transcription. We conclude that alternative splicing of MyD88 may provide a sensitive mechanism that ensures robust termination of inflammation for tissue repair and restoration of normal tissue homeostasis once an infection is controlled.

Motor protein-based active transport is essential for mRNA localization and local translation in animal cells, yet how mRNA granules interact with motor proteins remains poorly understood. Using an unbiased yeast two–hybrid screen for interactions between murine RNA-binding proteins (RBPs) and motor proteins, here we identified protein interaction with APP tail-1 (PAT1) as a potential direct adapter between zipcode-binding protein 1 (ZBP1, a β-actin RBP) and the kinesin-I motor complex. The amino acid sequence of mouse PAT1 is similar to that of the kinesin light chain (KLC), and we found that PAT1 binds to KLC directly. Studying PAT1 in mouse primary hippocampal neuronal cultures from both sexes and using structured illumination microscopic imaging of these neurons, we observed that brain-derived neurotrophic factor (BDNF) enhances co-localization of dendritic ZBP1 and PAT1 within granules that also contain kinesin-I. PAT1 is essential for BDNF-stimulated neuronal growth cone development and dendritic protrusion formation, and we noted that ZBP1 and PAT1 co-locate along with β-actin mRNA in actively transported granules in living neurons. Acute disruption of the PAT1–ZBP1 interaction in neurons with PAT1 siRNA or a dominant-negative ZBP1 construct diminished localization of β-actin mRNA but not of Ca2+/calmodulin-dependent protein kinase IIα (CaMKIIα) mRNA in dendrites. The aberrant β-actin mRNA localization resulted in abnormal dendritic protrusions and growth cone dynamics. These results suggest a critical role for PAT1 in BDNF-induced β-actin mRNA transport during postnatal development and reveal a new molecular mechanism for mRNA localization in vertebrates.