Annelies Wouters, Jan-Pieter Ploem, Sabine A. S. Langie, Tom Artois, Aziz Aboobaker, and Karen Smeets

Pluripotent stem cells hold great potential for regenerative medicine. Increased replication and division, such is the case during regeneration, concomitantly increases the risk of adverse outcomes through the acquisition of mutations. Seeking for driving mechanisms of such outcomes, we challenged a pluripotent stem cell system during the tightly controlled regeneration process in the planarian Schmidtea mediterranea. Exposure to the genotoxic compound methyl methanesulfonate (MMS) revealed that despite a similar DNA-damaging effect along the anteroposterior axis of intact animals, responses differed between anterior and posterior fragments after amputation. Stem cell proliferation and differentiation proceeded successfully in the amputated heads, leading to regeneration of missing tissues. Stem cells in the amputated tails showed decreased proliferation and differentiation capacity. As a result, tails could not regenerate. Interference with the body-axis-associated component β-catenin-1 increased regenerative success in tail fragments by stimulating proliferation at an early time point. Our results suggest that differences in the Wnt signalling gradient along the body axis modulate stem cell responses to MMS.

OBJECTIVE

To assess whether the risk of subsequent lower-limb amputations and death following an initial toe amputation among individuals with diabetes has changed over time and varies by demographic characteristics and geographic region.

PURPOSE

Although cesarean delivery is the most common surgical procedure in the United States, postoperative opioid prescribing varies greatly. We hypothesized that patient characteristics, procedural characteristics, or both would be associated with high vs low opioid use after discharge. This information could help individualize prescriptions.

Purpose:

In the phase III DUO trial, duvelisib, an oral dual PI3K-, inhibitor, demonstrated significantly improved efficacy versus ofatumumab [median (m) progression-free survival (PFS), 13.3 vs. 9.9 months (HR, 0.52; P < 0.0001); overall response rate [ORR], 74% vs. 45% (P < 0.0001)], with a manageable safety profile in patients with relapsed/refractory (R/R) chronic lymphocytic leukemia (CLL)/small lymphocytic lymphoma (SLL). We report results from patients with progressive disease (PD) after ofatumumab who crossed over to duvelisib in the DUO trial.

Tedizolid (TZD) and daptomycin (DAP) were assessed in a rat endocarditis model against Enterococcus faecalis, Enterococcus faecium (resistant to vancomycin and ampicillin), and Staphylococcus aureus. As a monotherapy, TZD for 5 days was not effective in a comparison with no-treatment controls, while DAP for 5 days was significantly effective against these bacteria. Step-down therapy (DAP for 3 days followed by TZD for 2 days) was as effective as DAP for 5 days and was comparable to 3 days of DAP plus ceftriaxone against all bacteria and to 3 days of DAP plus gentamicin against E. faecalis OG1RF.

In the third quarter of 2019, condotel and resort villa market saw both supply and consumption falling by double digits since the ‘Ghost month’ made investors hesitate.

Bearing in mind that maintaining a united front against the virus can bode well for the country.

As they implement stricter measures and distribute millions worth of donations to communities in need.

Hearing the kingdom’s cry for help, these developers are offering their resources to everyone in need.

The Vietnam’s real estate market has clearly seen signs of a downturn in many segments since the beginning of 2018. So how to gain profit from your realty investment?

Investing in homestay properties has emerged as a new trend in Vietnam. In order to maximize their profit in the long term, investors should take into account a number of factors.

If you're 13 to 24 years old and love to make movies, you'll want to enter the Windsor Youth Short Film Showcase next week. Organizer Gemma Eva says the project is meant to spotlight local "Gen-Z filmmakers."

Crawling on his belly through a sewer pipe beneath the streets of Warsaw, Poland, with a battle raging overhead, 16-year-old Lucjan Krause could scarcely have imagined he would survive the fighting, let alone go on to build a globally admired atomic physics program at the University of Windsor. Now 92 and still in full command […]

A self-powered device that generates electricity from the wind can also absorb and break down harmful nitric oxide and nitrogen dioxide from the air

In winter, the veins of leaves don’t get completely covered in frost. Mimicking this effect could be the best way to create ice-resistant surfaces

A 3D printed grate can be used to make a smart window that blocks heat from sunlight out in the summer while letting it through in the winter, conserving energy

Please, I implore you: jump on this bandwagon.

A space flight start-up called Astra is about to attempt to launch two small rockets into orbit over a few weeks to win $12 million from the US military

White dwarf stars are common in the galaxy, but astronomers have found one that doesn't seem to obey the rules. They think it was born when two smaller white dwarfs merged together

Venus’s surface is covered in strange wiggling lines that may form when colossal stacks of lava are carved by gentle winds over hundreds of millions of years

Brown dwarfs, which are halfway between huge planets and small stars, have extremely powerful winds whipping around them at speeds of about 650 metres per second

It's about more than just first place as teams look toward more competitions in 2020

Billionaire Alex Waislitz stands to make millions after two financial services companies agreed to merge.

This is a video of musician and Instagrammer nathanleighsays performing Saria's Song (the Lost Woods theme) from The Legend Of Zelda: Ocarina Of Time on 24 partially empty wine bottles. How about that! Of course this would never work at my aunt's house on account of the bottles all playing the same note because they're empty. And the cats! Soooooo many cats. Keep going for the performance as well as a clip of Link learning Saria's song from the actual game.

Epic will be hosting its latest in-game event in Fortnite this weekend, featuring the talents of Dillon Francis, Steve Aoki and deadmau5

Chris learns a hard lesson about how free-to-play isn’t really free. Alice learns how to import Gunpla games. Alex learns how to properly cross check...

PEOPLE who are not positive for Covid-19 could be told to isolate from their families before test results are issued as a precaution, Scotland’s national clinical director has warned.

Donald Trump takes a jab at the South Korean film Parasite, best picture at this year's Oscars, telling supporters in Colorado that the US has 'enough problems with South Korea', and: ‘Can we get Gone With the Wind back?’ He also dismisses Brad Pitt, who – during his Oscars speech said his 45-second slot was more than John Bolton received at the US president's Senate impeachment trial. Trump calls the actor a 'little wise guy'

• Parasite's best picture Oscar could kickstart a new era of internationalism
  

It’s only the beginning of May, but for local golfers and tennis players, Monday felt like Christmas. Golf courses and tennis courts were officially allowed to open Monday as a part ...

The winners and results of last night's Mega Millions lottery, plus how to avoid falling victim to a lottery scam.

Twitch is doubling down on esports in this new era of social distancing as a number of traditional sporting events have been cancelled. The company this week introduced a new esports directory on its site that will make it easier for viewers to find live matches, information on players, games with active competition leagues, a […]

“The rapid & global spread of coronavirus is a devastating reminder of the consequences of global disorder”: Tharoor

Oscar-winning Iranian director Asghar Farhadi said Sunday he will not attend the Academy Awards on Feb. 26, “even if exceptions were to be made for my trip,” as protests over President Donald Trump's immigration ban spread to Hollywood's biggest global stage.

HARRY KANE is now the clear favourite to win the World Cup Golden Boot after his hat-trick against Panama..

We reckon it's pretty damn good. Here's your chance to win one.

Winfrey eventually walked away from the Simmons project and had her name removed as a producer, citing creative differences.

The 87-year-old record producer - who worked extensively with Michael at the height of his pop career - was originally awarded

Hurd and Morris wed in 2018 after five years together.

Jordan Goudreau once pushed a plan to protect U.S. schools. Then he moved on to a more daring pursuit, which also didn't end well.

America can take its next steps toward reopening by placing an emphasis on emerging health data and closely examining how early states are performing, Housing and Urban Development Secretary Dr. Ben Carson asserted Saturday.

The Washington Capitals announced Friday that forward Brendan Leipsic was placed on unconditional waivers after his private messages were leaked on social media Wednesday.