There are (at least) 9 things that you used to be able to do on a cruise that you are not able to do any more. Discover what things cruisers are not allowed to do on a cruise ship. Whilst some may be obvious, there are quite a few surprises. These cruise tips, secrets and facts will, hopefully, entertain as well as inform you. How many things did you not know you are not allowed to do on your cruise and cruising vacation now?

Do you have a cruise or travel bucket list? If so, should a French Polynesia cruise be on that list? In this I discuss the Tahiti and French Polynesia region and review what I think are the 4 pros and the 4 cons of cruising that I believe you should consider as you consider this as a cruising vacation option. I travelled to the region, which is pretty remote to explore. It takes well over 8 hours to fly from Los Angeles and the trip from Europe is 21 hours. It is very unique, different and beautiful. But, is it worth going all the way there? Find out what to expect and if I think you should add this to your travel and cruise bucket list - especially when there are so many other things you could have on your travel and cruise bucket list.

Note: Some of the costs of my trip to French Polynesia were covered by Paul Gauguin Cruises and Air Tahiti Nui before making this video.

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Gary Bembridge's Tips For Travellers aims to help you make more of your precious travel time and money on land and when cruising the oceans or rivers of the world. To help you, in every video I draw on my first-hand tips and advice from travelling every month for over 20 years and 60+ cruises.

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Saga Cruises launched Spirit of Discovery in July 2019, the first of two sister cruise ships (Spirit of Adventure in 2020). These are the first new-build ships for cruising ever for Saga, why had previously bought and refurbished existing cruise ships. The Spirit of Discovery holds 999 passengers and in this I discuss the 8 things that I think are best about the ship, and show and explain why. I also talk about one major watch-out and issue that could stop you from cruising.

Note: I travelled as a guest of the cruise line on a pre-naming cruise before making this video. Saga Cruises had no input in the video and content.

** Subscribe to my channel: http://bit.ly/TFT_YouTube2
** Buy one of my unique Cruise T-shirts: http://bit.ly/TFTStore
** Get great cruise deals via CRUISEDIRECT.COM: http://bit.ly/TFTBookCruise

Gary Bembridge's Tips For Travellers aims to help you make more of your precious travel time and money on land and when cruising the oceans or rivers of the world. To help you, in every video I draw on my first-hand tips and advice from travelling every month for over 20 years and 60+ cruises.

Follow Tips For Travellers on:
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Discover the 10 things that you should never do on a cruise ship balcony. Some of them are rules from the cruise line, which if you break you could find yourself thrown off your cruising vacation (or even banned by the cruise line for life!). Some are things that will make your and the other cruisers around you have a more enjoyable cruise. And some even could save you a lot of embarrassment....

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** UK Cruisers get great cruise deals with CRUISE.CO.UK: http://bit.ly/BookCruiseUK

Gary Bembridge's Tips For Travellers aims to help you make more of your precious travel time and money on land and when cruising the oceans or rivers of the world. To help you, in every video I draw on my first-hand tips and advice from travelling every month for over 20 years and 60+ cruises.

Follow Tips For Travellers on:
- Instagram: http://www.instagram.com/garybembridge
- Facebook: http://www.facebook.com/tipsfortravellers
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How can you get free things on your next cruise, and get much more bang for your cruising bucks? I discuss the 6 main areas of cruise freebies that are available, if you go and ask for them or seek them out. They are not things that cruise brochures and websites usually reveal, or talk about. I tell you the things to focus on and how to get as many free things as you possibly can, including drinks, special food, gifts, spa treatments, better and improved cabins and more.

** Buy my Cruise T-shirts: http://bit.ly/TFTStore
** USA cruisers get great cruise deals CRUISEDIRECT.COM: http://bit.ly/TFTBookCruise
** UK Cruisers get great cruise deals with CRUISE.CO.UK: http://bit.ly/BookCruiseUK

Gary Bembridge's Tips For Travellers aims to help you make more of your precious travel time and money on land and when cruising the oceans or rivers of the world. To help you, in every video I draw on my first-hand tips and advice from travelling every month for over 20 years and 60+ cruises.

Follow Tips For Travellers on:
- Instagram: http://www.instagram.com/garybembridge
- Facebook: http://www.facebook.com/tipsfortravellers
- Twitter: http://www.twitter.com/garybembridge

I am not suggesting, or recommending, you should sneak booze on your next next cruising vacation as you could, in worst case, risk being refused boarding if caught. However despite this, there are cruisers who use surprising and unusual techniques to smuggle alcohol on to a cruise ship to avoid having to buy drinks on board. In this video I explore the different ways that people are using to sneak their alcohol onto their cruise ship. The cost of drinking on a cruise is increasing, and with some of the drinks packages you could be spending almost as much as the cruise itself costs and some travellers are resorting to innovative methods to bring their own alcohol onto cruise ships. There are even companies making products, devices and accessories specifically designed to assist.

Is Saga Cruises the best cruise line for your cruise vacation? In this I explore what I think Saga Cruises do better, the same or worse than other premium cruise lines. I also look at who they are and who they are most suited for and why. Saga Cruises is a UK-based line with a different take on a number of areas of cruising, which you will discover...

Note: I travelled as a guest of the cruise line a number of times over the last few years before making this video

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Buying my Cruise T-shirts: http://bit.ly/TFTStoreBooking your next cruise with CRUISEDIRECT.COM: http://bit.ly/TFTBookCruise

Gary Bembridge's Tips For Travellers aims to help you make more of your precious travel time and money on land and when cruising the oceans or rivers of the world. To help you, in every video I draw on my first-hand tips and advice from travelling every month for over 20 years and 60+ cruises.

Follow Tips For Travellers on:
- Instagram: http://www.instagram.com/garybembridge- Facebook: http://www.facebook.com/tipsfortravellers

- Twitter: http://www.twitter.com/garybembridge

There are ways to curb the pain of economic and political changes.

Young medical students and health-care workers simply want to keep contributing in the only home they know.

strataconf: A roundup from the data journalism beat http://t.co/y8RVUwHO4G Global open data, scholarships, mapping a civil war & more #strataconf

strataconf: Innovative ways journalists are using data to tell stories http://t.co/y8RVUwHO4G Global open data, scholarships, mapping a civil war & more

strataconf: The Future Is Graph Databases http://t.co/BVxOZwtoKS A Conversation with @EmilEifrem, founder of @Neo4J #strataconf

The communications regulator is asking telecom companies to consider lowering the cost of data in a bid to ensure productivity during the period the coronavirus outbreak.

This one-pan chicken dinner is made with potatoes, onions, garlic, cherry tomatoes, and an easy paprika sauce. Dairy-free, gluten-free. 1 hour start to finish.

Continue reading "One-Pan Paprika Chicken with Potatoes and Tomatoes" »

velocityconf: @tsantero @garethr No, there's just a lot that goes into producing #velocityconf. Plus the chairs are getting ready for Santa Clara + NY! :)

With the working class resorting to online vouchers for groceries and the poor eating less bread than before, is it not impractical for the president to expect ordinary citizens to tighten their belts when MPs, Ministers and government officials' well-fed bellies belie the trouble the economy is in?

South Africans would do well to verify information which they receive through social media channels, and avoid re-posting or forwarding information which they have not properly verified.

Edmonton and Jasper, Alberta Canada

Lake Louise, Banff and Calgary, Alberta Canada

Baja California

Hear about Trekking the Annapurna Circuit in Nepal as the Amateur Traveler talks to the Su Family from sufamilyadventures.com about tackling this well known route.

Hear about travel to Oklahoma City as the Amateur Traveler talks to Sheila Scarborough from Tourism Currents about what she calls a "tremendously underrated destination".

An evangelical church network in California will ignore Democratic Gov. Gavin Newsom’s executive prohibitions this month, opening sanctuary doors for communal worship despite the ongoing coronavirus pandemic. According to Fox News, California Church United, representing roughly 3,000 churches and 2.5 million congregants statewide, announced Thursday that it will return to conducting in-person services by Pentecost…

The post Huge Group of California Churches Announces Plan To Defy Governor Newsom’s Order appeared first on The Western Journal.

It’s not good for people to be alone. Quarantining has been difficult for many people who have gotten used to socializing daily, and because of that, some have found ways to follow the rules but feel a little less isolated. Some neighborhoods have decided to be social while maintaining the suggested distance apart, whether that’s…

The post ‘The Voice’ Contestant Alexa Cappelli Treats Neighbors to Weekly Performance in Her Cul-de-Sac appeared first on The Western Journal.

At least 65,116 individuals have been "forcibly disappeared" by the Syrian government, according to a new report by Amnesty International.

As ISIS expands its reach into Afghanistan, it is training children and teenagers to become the next generation of jihadis.

Striso is a digital instrument with pressure-sensitive buttons that plays a variety of acoustic-like sounds.  Read more...

TL;DR: Get trained in cybersecurity with The Ultimate 2020 White Hat Hacker Certification Bundle for $39.90, a 97% as of May 9.

When you think of superheroes, you probably picture capes and some sort of otherworldly powers. But in the digital world, superheroes are actually normal people like you and me. They just happen to know a thing or two about stopping cybercriminals in their tracks.

These superheroes are in short supply across the world. In fact, there are over half a million cybersecurity job openings in the U.S. alone. That just means this could be your chance to swoop in, superhero style, and save the day. But first things first, you need to learn the ropes and the Ultimate 2020 White Hat Hacker Certification Bundle is a great place to start. Read more...

I have a love/hate relationship with musical reaction videos. There are kids reacting to heavy metal (or The Beatles), vocal teachers and opera singers reacting to rock vocalists, millennials reacting to classic rock, and on and on. Some of these are quite moving, for instance, a 20-something hearing Zep's "Since I've Been Loving You" for the first time or a vocal teacher reacting to a Black Metal vocalist's cookie monster growl.

In these two videos, Heline, a classical flutist and music teacher, listens to Jethro Tull's Ian Anderson performing "Bouree" at a 1969 concert and then she listens and reacts to a performance from 1976.

I didn't realize that Anderson had only been playing the flute for a year and had no formal training at the time of the 69 video (their first tour). Heline can appreciate his chutzpah in the first performance and the fact that he's only been playing for a year(!), but is perhaps predictably critical of his playing. She is more impressed with what she sees and hears in the 76 performance.

Personally, I always thought his playing was inspired and his playing, vocalizing, singing and playing, and his other stage antics (the goofy one-legged stance) were a perfect example of "the street finding its own uses for things," using technology in ways in which it was never intended. As with all things artistic, your mileage may vary.

Image: YouTube Read the rest

The Vuelta a Espana organisers have confirmed that a race already rescheduled and shortened would also now cut its foray into neighbouring Portugal from the 2020 itinerary.

Police officers responding to a call about a man with a machete in Oppenheimer Park Friday afternoon found what they were looking for, and then some.

T.J. Lovell had just 30 minutes to pack up his belongings from the tent city in Oppenheimer Park if he wanted access to a hotel room that he could share with his father.

The following article, Donald Trump Jr. Offers To Walk Bride-to-Be Down The Aisle After Liberal Parents Refuse To Attend Her Wedding Because Fiance Is A Conservative, was first published on 100PercentFedUp.com.

TDS or Trump Derangement Syndrome brings out the worst in a lot of people. One young Texan girl’s story of her intolerant parents who refuse to attend her wedding because she’s marrying a conservative, however, is a new low. Lawyer and conservative civil rights activist, Rogan O’Handley tweeted a Tik Tok video of a Texan […]

Continue reading: Donald Trump Jr. Offers To Walk Bride-to-Be Down The Aisle After Liberal Parents Refuse To Attend Her Wedding Because Fiance Is A Conservative ...

The following article, Legal Immigrant in Michigan Sends a Message to MI Gov Whitmer and Media Criticizing Freedom-Loving Protesters, was first published on 100PercentFedUp.com.

The media and even some governors are trying to paint the Americans who are protesting to open businesses up as racists and even Nazis. The Governor of Michigan recently made a derogatory statement about the protesters implying they are racists. Painting a negative picture of the people who want their freedom and businesses demonizes our […]

Continue reading: Legal Immigrant in Michigan Sends a Message to MI Gov Whitmer and Media Criticizing Freedom-Loving Protesters ...

The following article, Video: Dallas Salon Owner Shelley Luther Gets Surprise Visit from Sen Ted Cruz for a Celebratory Haircut, was first published on 100PercentFedUp.com.

When Dallas, Texas salon owner Shelley Luther opened her salon in defiance of the lockdown order in Texas, she was visited numerous times by the local police and then sentenced to 7 days in jail with a fine of $7,000. After public outrage at her punishment, the Texas Supreme Court stepped in to demand her […]

Continue reading: Video: Dallas Salon Owner Shelley Luther Gets Surprise Visit from Sen Ted Cruz for a Celebratory Haircut ...

Telomeres are specific nucleoprotein structures that are located at the ends of linear eukaryotic chromosomes and play crucial roles in genomic stability. Telomere DNA consists of simple repeats of a short G-rich sequence: TTAGGG in mammals and TTTAGGG in most plants. In recent years, the mammalian telomeric G-rich repeats have been shown to form G-quadruplex (G4) structures, which are crucial for modulating telomere functions. Surprisingly, even though plant telomeres are essential for plant growth, development, and environmental adaptions, only few reports exist on plant telomeric G4 DNA (pTG4). Here, using bulk and single-molecule assays, including CD spectroscopy, and single-molecule FRET approaches, we comprehensively characterized the structure and dynamics of a typical plant telomeric sequence, d[GGG(TTTAGGG)3]. We found that this sequence can fold into mixed G4s in potassium, including parallel and antiparallel structures. We also directly detected intermediate dynamic transitions, including G-hairpin, parallel G-triplex, and antiparallel G-triplex structures. Moreover, we observed that pTG4 is unfolded by the AtRecQ2 helicase but not by AtRecQ3. The results of our work shed light on our understanding about the existence, topological structures, stability, intermediates, unwinding, and functions of pTG4.

Type 2 diabetes mellitus (T2DM) is characterized by impaired glucose-stimulated insulin secretion and increased peripheral insulin resistance. Unremitting endoplasmic reticulum (ER) stress can lead to beta-cell apoptosis and has been linked to type 2 diabetes. Although many studies have attempted to link ER stress and T2DM, the specific effects of ER stress on beta-cell function remain incompletely understood. To determine the interrelationship between ER stress and beta-cell function, here we treated insulin-secreting INS-1(832/13) cells or isolated mouse islets with the ER stress–inducer tunicamycin (TM). TM induced ER stress as expected, as evidenced by activation of the unfolded protein response. Beta cells treated with TM also exhibited concomitant alterations in their electrical activity and cytosolic free Ca2+ oscillations. As ER stress is known to reduce ER Ca2+ levels, we tested the hypothesis that the observed increase in Ca2+ oscillations occurred because of reduced ER Ca2+ levels and, in turn, increased store-operated Ca2+ entry. TM-induced cytosolic Ca2+ and membrane electrical oscillations were acutely inhibited by YM58483, which blocks store-operated Ca2+ channels. Significantly, TM-treated cells secreted increased insulin under conditions normally associated with only minimal release, e.g. 5 mm glucose, and YM58483 blocked this secretion. Taken together, these results support a critical role for ER Ca2+ depletion–activated Ca2+ current in mediating Ca2+-induced insulin secretion in response to ER stress.

Neoantimycins are anticancer compounds of 15-membered ring antimycin-type depsipeptides. They are biosynthesized by a hybrid multimodular protein complex of nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS), typically from the starting precursor 3-formamidosalicylate. Examining fermentation extracts of Streptomyces conglobatus, here we discovered four new neoantimycin analogs, unantimycins B–E, in which 3-formamidosalicylates are replaced by an unusual 3-hydroxybenzoate (3-HBA) moiety. Unantimycins B–E exhibited levels of anticancer activities similar to those of the chemotherapeutic drug cisplatin in human lung cancer, colorectal cancer, and melanoma cells. Notably, they mostly displayed no significant toxicity toward noncancerous cells, unlike the serious toxicities generally reported for antimycin-type natural products. Using site-directed mutagenesis and heterologous expression, we found that unantimycin productions are correlated with the activity of a chorismatase homolog, the nat-hyg5 gene, from a type I PKS gene cluster. Biochemical analysis confirmed that the catalytic activity of Nat-hyg5 generates 3-HBA from chorismate. Finally, we achieved selective production of unantimycins B and C by engineering a chassis host. On the basis of these findings, we propose that unantimycin biosynthesis is directed by the neoantimycin-producing NRPS–PKS complex and initiated with the starter unit of 3-HBA. The elucidation of the biosynthetic unantimycin pathway reported here paves the way to improve the yield of these compounds for evaluation in oncotherapeutic applications.

Invitation Only Research Event

5 August 2016

Based on a workshop which played out a scenario of rising tensions between Turkey and Russia, this paper finds that the situation would have to escalate dramatically to threaten transatlantic unity.

9 December 2019

23 April 2020

Junmei Zhang
Feb 1, 2009; 8:215-225
Research

Sean R. Collins
Mar 1, 2007; 6:439-450
Research

Jesper V. Olsen
Dec 1, 2005; 4:2010-2021
Technology

Treatment of patients with chronic lymphocytic leukemia (CLL) with inhibitors of Bruton's tyrosine kinase (BTK), such as ibrutinib, is limited by primary or secondary resistance to this drug. Examinations of CLL patients with late relapses while on ibrutinib, which inhibits BTK's catalytic activity, revealed several mutations in BTK, most frequently resulting in the C481S substitution, and disclosed many mutations in PLCG2, encoding phospholipase C-γ2 (PLCγ2). The PLCγ2 variants typically do not exhibit constitutive activity in cell-free systems, leading to the suggestion that in intact cells they are hypersensitive to Rac family small GTPases or to the upstream kinases spleen-associated tyrosine kinase (SYK) and Lck/Yes-related novel tyrosine kinase (LYN). The sensitivity of the PLCγ2 variants to BTK itself has remained unknown. Here, using genetically-modified DT40 B lymphocytes, along with various biochemical assays, including analysis of PLCγ2-mediated inositol phosphate formation, inositol phospholipid assessments, fluorescence recovery after photobleaching (FRAP) static laser microscopy, and determination of intracellular calcium ([Ca2+]i), we show that various CLL-specific PLCγ2 variants such as PLCγ2S707Y are hyper-responsive to activated BTK, even in the absence of BTK's catalytic activity and independently of enhanced PLCγ2 phospholipid substrate supply. At high levels of B-cell receptor (BCR) activation, which may occur in individual CLL patients, catalytically-inactive BTK restored the ability of the BCR to mediate increases in [Ca2+]i. Because catalytically-inactive BTK is insensitive to active-site BTK inhibitors, the mechanism involving the noncatalytic BTK uncovered here may contribute to preexisting reduced sensitivity or even primary resistance of CLL to these drugs.