A new technique helps anesthesiologists track changes in states of consciousness

Consumers thinking of dining out for their next meal may want to consider one instruction for the server -- hold the salt.

It’s a wrap! The Texas Advanced Computing Center (TACC) at UT Austin welcomed more than 100 participants for the 7th annual TACC Symposium for Texas Researchers (TACCSTER). The event exists […]

The post Research Insights, HPC Expertise, Meaningful Collaborations Abound at TACCSTER 2024 appeared first on HPCwire.

What makes for a meaningful life? Social psychologist Brian S. Lowery explores three ideas tied to the experience of meaning and shows why simply pursuing personal achievements isn't the best way to find it.

Sara Giusto is a talent manager, but not in the sense you might imagine. Her biggest client is imma, an influencer with pink hair ... who isn't human. Giusto discusses what the rise of "virtual humans" means for the real world — and invites imma onstage to explore an important question: In an increasingly digital world, what's really real?

Looking to infuse more connection into your time with friends and family? Facilitator Priya Parker urges us to approach gathering with intention and creativity. Diving into the evolving significance of coming together both in-person and virtually, she demystifies the role of a host, outlines mistakes we should avoid and shares the potential of thoughtfully designed get-togethers to bring lasting change to communities, workplaces and personal relationships. (This conversation was hosted by TED's Cloe Shasha Brooks. Visit ted.com/membership to support TED today and join more exclusive events like this one.)

We tend to consider romantic partners and family ties to be our most important relationships, but deep friendships can be just as meaningful. In a perspective-shifting talk, author Rhaina Cohen introduces us to the people unsettling norms by choosing a friend as a life partner — and shows why we're all better off recognizing there's more than one kind of significant other.

Groups on all sides of the debate over private school choice agree that a U.S. Supreme Court ruling will be tremendously consequential. But it may take some time for the ripple effects to spread.

Young people deserve a say in how to keep their communities safe, writes high school student M’munga Songolo.

Some states, such as California, Kentucky and North Dakota plan to use the Every Student Succeeds Act to bolster the decision-making powers of their local school boards in the coming years.

Two infectious disease experts weigh in on how a COVID-19 vaccine that’s 90 percent effective, as early results are showing, could change school health and safety protocols.

The extensions will let schools and community groups continue feeding students with fewer restrictions than typical under the National School Lunch and Breakfast Programs.

Districts that can screen, interview, and select candidates virtually will have less disruption to their hiring, despite how coronavirus is upending every aspect of school operations.

A school cook in Nebraska was canned after he mixed kangaroo meat into chili made for students.

Virginia Gov.-elect Ralph Northam has said he would further restrict that state's charter laws, and New Jersey Gov.-elect Phil Murphy has promised to pull the state out of the PARCC testing consortium.

"Aloha" isn't just a greeting; in a way it is their way of life, and when you distort that sacred word, you distort their way of life.

Idaho officials asked a U.S. Supreme Court justice to block an injunction that allows a group backing an education ballot initiative to collect electronic signatures because of COVID-19.

Neuronal cytotoxic edema is implicated in neuronal injury and death, yet mitigating brain edema with osmotic and surgical interventions yields poor clinical outcomes. Importantly, neuronal swelling and its downstream consequences during early brain development remain poorly investigated, and new treatment approaches are needed. We explored Ca²⁺-dependent downstream effects after neuronal cytotoxic edema caused by diverse injuries in mice of both sexes using multiphoton Ca²⁺ imaging in vivo [Postnatal Day (P)12–17] and in acute brain slices (P8–12). After different excitotoxic insults, cytosolic GCaMP6s translocated into the nucleus after a few minutes in a subpopulation of neurons, persisting for hours. We used an automated morphology-detection algorithm to detect neuronal soma and quantified the nuclear translocation of GCaMP6s as the nuclear to cytosolic intensity (N/C ratio). Elevated neuronal N/C ratios occurred concurrently with persistent elevation in Ca²⁺ loads and could also occur independently from neuronal swelling. Electron microscopy revealed that the nuclear translocation was associated with the increased nuclear pore size. The nuclear accumulation of GCaMP6s in neurons led to neocortical circuit dysfunction, mitochondrial pathology, and increased cell death. Inhibiting calpains, a family of Ca²⁺-activated proteases, prevented elevated N/C ratios and neuronal swelling. In summary, in the developing brain, we identified a calpain-dependent alteration of nuclear transport in a subpopulation of neurons after disease-relevant insults leading to long-term circuit dysfunction and cell death. The nuclear translocation of GCaMP6 and other cytosolic proteins after acute excitotoxicity can be an early biomarker of brain injury in the developing brain.

Global exchange of information on compliance with national, regional, and international fisheries laws and regulations governing sustainable fishing is now possible with the launch of the Global [...]

The holiday was used as a call for freedom during the civil rights movement and as a day of mourning by Native Americans.

Paul Theroux's Quest to Define Hawaii: http://j.mp/HPVhp8 For Hawaiians, both native and those who have made it their adopted home, the Hula is more than just a dance, it is a artistic representation of the islands themselves

Biographer David Maraniss says that in order to truly understand Clemente's importance to the sport, you have to look beyond his spectacular numbers

A "staggering array" of markings have been hiding in plain sight carved into the walls of Gainsborough Old Hall, a 500-year-old home in Lincolnshire, England

Mosquitoes take blood meals from a diverse range of host animals and their host associations vary by species. Characterizing these associations is an important element of the transmission dynamics of mosquito-vectored pathogens. To characterize mosquito host associations, various molecular techniques have been developed, which are collectively referred to as blood meal analysis. DNA barcoding has diverse biological applications and is well-suited to mosquito blood meal analysis. The standard DNA barcoding marker for animals is a 5' fragment of the cytochrome c oxidase I (COI) gene. A major advantage of this marker is its taxonomic coverage in DNA sequence reference databases, making it feasible to identify a wider range of mosquito host species than with any other gene. However, the COI gene contains high sequence variation at potential priming sites between vertebrate orders. Coupled with the need for primer sequences to be mismatched with mosquito priming sites so that annealing to mosquito DNA is inhibited, it can be difficult to design primers suitable for blood meal analysis applications. Several primers are available that perform well in mosquito blood meal analysis, annealing to priming sites for most vertebrate host taxa, but not to those of mosquitoes. Because priming site sequence variation among vertebrate taxa can cause amplification to fail, a hierarchical approach to DNA barcoding-based blood meal analysis can be applied. In such an approach, no single primer set is expected to be effective for 100% of potential host species. If amplification fails in the initial reaction, a subsequent reaction is attempted with primers that anneal to different priming sites, and so on, until amplification is successful.

Mosquito species vary in their host associations. Although some species are relative generalists, most specialize, to varying extents, on particular types of host animals. Mosquito host associations are among the most important factors that influence the transmission dynamics of mosquito-vectored pathogens, and understanding these associations can provide insight on how such pathogens move within ecosystems. Characterization of the host associations of mosquito species requires applying blood meal analysis to the largest possible sample size of mosquito blood meals. Processing large samples of mosquito blood meals can be time-consuming, especially when chain-termination sequencing is used, necessitating individual processing of each specimen. Various methods and commercially available kits and products are available for extracting DNA from mosquito blood meals. The hot sodium hydroxide and Tris (HotSHOT) method is a rapid and inexpensive method of DNA extraction that is compatible with the recovery of DNA from mosquito blood meals preserved on QIAcard Flinders Technology Associates (FTA) Classic Cards (FTA cards). FTA cards allow nucleic acids found in blood meals to be preserved easily, even in field conditions. DNA prepared using this method is suitable for polymerase chain reaction (PCR)-based blood meal analysis.

All PCR- and DNA-based blood meal analyses require host DNA from a mosquito blood meal to be effectively preserved between the time when the specimen is collected and the extraction of DNA. As soon as a mosquito ingests blood from a host animal, digestion of host cells and cellular components within the blood meal by enzymes in the mosquito midgut begins to degrade the host DNA templates that are the targets of polymerase chain reaction (PCR) amplification. Without effective preservation, host DNA is typically undetectable by PCR 48 h after feeding, because of digestion. Preservation methods for mosquito blood meals vary in their efficacy, and the logistics of fieldwork can limit the options for preservation of blood meals and maintenance of the integrity of host DNA. This protocol describes a method of blood meal preservation that is effective, convenient, and amenable to fieldwork in remote locations where cryopreservation at –20°C or –80°C may not be feasible. It uses a Flinders Technology Associates (FTA) card, which is a chemically treated card that lyses cells and allows nucleic acids to be preserved. This method is also expected to preserve the DNA or RNA of pathogens present within the engorged mosquito abdomen, including RNA viruses.

With president-elect Donald Trump promising to drill in Alaska's Arctic National Wildlife Refuge, the Gwich'in are preparing to fight for the Porcupine caribou herd.

There have been 25 confirmed measles cases in New Brunswick since October after the identification of 11 new cases, according to a news release Saturday from Public Health.

A museum in Fort McMurray is helping visitors gain a deeper understanding of Canada’s role in previous wars. The Wood Buffalo Military Museum Centre is offering a chance to get up close to military artifacts. Young visitors say seeing the remnants of conflict gives them a new understanding of Remembrance Day's meaning.

Wildland firefighters were at Queen’s Park on Wednesday to outline why they feel a new bill that extends protections for cancers, heart injuries and post-traumatic stress disorder linked to their occupation falls short.

Chair Rick Petrella and trustees Dan Dignard, Bill Chopp and Mark Watson of the Brant Haldimand Norfolk Catholic District School Board were to Italy between July 8 and 15 to buy $100,000 in custom artwork for two new high schools. It's among trips by Ontario school boards that have come under scrutiny for the way public money is spent.

Carleton University will soon need to take 'significant measures' to balance its budget, according to a letter the Ottawa institution recently sent out to staff and students.

Some Ukrainians living in Calgary say they're even more worried for their friends and family back home in light of Donald Trump's election win.

The host associations of mosquitoes vary by species, with some species being relative generalists, whereas others specialize, to varying extents, on a particular subset of the available host community. These host associations are driving factors in transmission dynamics of mosquito-vectored pathogens. For this reason, characterizing the host associations of mosquito species is critical for understanding the epidemiology of mosquito-vectored pathogens. Diverse methods have been used to associate mosquito species with their hosts. These typically include collecting mosquitoes that bite a restrained host (bait) or collecting wild blood-engorged mosquitoes and matching their blood meal to reference samples (blood meal analysis). Blood meal analysis refers to a collection of molecular techniques for determining the taxonomic identity of the source of a mosquito blood meal using cytological, serological, or DNA-based characteristics of the blood meal. Blood meal analyses that are based on DNA markers have advantages over cytological and serological methods and are effective for determining species-level identities of hosts from a broad range of potential host taxa. Here, we discuss effective techniques for analyzing blood meals.

Customized valve and cylinder maker sees 420 percent increase in online transactions in the first three months

New product for intensive care units automates costly and time-consuming nursing work

Donald Trump's victory in the 2024 US presidential election is likely to usher in a raft of economic changes at home and abroad, touching everything from foreign trade to the independence of the US central bank.

The Creative Arts team perform their Christmas show for school children all over Ireland in the course of three weeks.

A public baptism ceremony at the river becomes an opportunity to teach an onlooker about the true meaning of baptism.

A Christian football coach experiences what he says is the best gift from God, something “so much more than winning”.

It matters not if we are from 200 countries; we are one in Christ and shall be for eternity. OMNI-team member Greg Kernaghan about ‘globalisation’.

A Crimean Tatar man shares how he gained courage and learnt vital truths through reading Into the Den of the Infidels, produced by OM EAST.

"In order to fulfil our mandate, there are several needs we must invest in," says Stephan Bauer.

In this Facebook Live discussion, Education Week reporters Alyson Klein and Andrew Ujifusa discuss President Trump's budget, and what it means for public education.

Native-language assessments may more fully reflect what English-language learners know and can do academically after months away from school. But not all states offer them.

Would measurement approaches based on "learning growth" resolve some of the concerns over NCLB?

High-quality professional learning is difficult to provide in education, principal Jasmine Kullar writes. Here's a solution.

The justices hear arguments Nov. 12 on the Trump administration's effort to end deportation relief under Deferred Action for Childhood Arrivals, in a case pitting the administration and GOP-leaning states against a host of education and advocacy groups.

Idaho officials asked a U.S. Supreme Court justice to block an injunction that allows a group backing an education ballot initiative to collect electronic signatures because of COVID-19.