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Small-angle neutron scattering (SANS) and spin-echo SANS measurements reveal the logarithmic fractal structure of the large-scale chromatin organization in HeLa nuclei

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Forthcoming article in Acta Crystallographica Section B Structural Science, Crystal Engineering and Materials




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Open-access and free articles in Acta Crystallographica Section F: Structural Biology and Crystallization Communications





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Structure of Thermococcus litoralis Δ1-pyrroline-2-carboxylate reductase in complex with NADH and l-proline

l-Hydroxyproline (l-Hyp) is a nonstandard amino acid that is present in certain proteins, in some antibiotics and in the cell-wall components of plants. l-Hyp is the product of the post-translational modification of protein prolines by prolyl hydroxylase enzymes, and the isomers trans-3-hydroxy-l-proline (T3LHyp) and trans-4-hydroxy-l-proline (T4LHyp) are major components of mammalian collagen. T4LHyp follows two distinct degradation pathways in bacteria and mammals, while T3LHyp is metabolized by a two-step metabolic pathway that is conserved in bacteria and mammals, which involves a T3LHyp dehydratase and a Δ1-pyrroline-2-carboxylate (Pyr2C) reductase. In order to shed light on the structure and catalysis of the enzyme involved in the second step of the T3LHyp degradation pathway, the crystal structure of Pyr2C reductase from the archaeon Thermococcus litoralis DSM 5473 complexed with NADH and l-proline is presented. The model allows the mapping of the residues involved in cofactor and product binding and represents a valid model for rationalizing the catalysis of Pyr2C reductases.




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Structure–function study of AKR4C14, an aldo-keto reductase from Thai jasmine rice (Oryza sativa L. ssp. indica cv. KDML105)

Aldo-keto reductases (AKRs) are NADPH/NADP+-dependent oxidoreductase enzymes that metabolize an aldehyde/ketone to the corresponding alcohol. AKR4C14 from rice exhibits a much higher efficiency in metabolizing malondialdehyde (MDA) than do the Arabidopsis enzymes AKR4C8 and AKR4C9, despite sharing greater than 60% amino-acid sequence identity. This study confirms the role of rice AKR4C14 in the detoxification of methylglyoxal and MDA, and demonstrates that the endogenous contents of both aldehydes in transgenic Arabidopsis ectopically expressing AKR4C14 are significantly lower than their levels in the wild type. The apo structure of indica rice AKR4C14 was also determined in the absence of the cofactor, revealing the stabilized open conformation. This is the first crystal structure in AKR subfamily 4C from rice to be observed in the apo form (without bound NADP+). The refined AKR4C14 structure reveals a stabilized open conformation of loop B, suggesting the initial phase prior to cofactor binding. Based on the X-ray crystal structure, the substrate- and cofactor-binding pockets of AKR4C14 are formed by loops A, B, C and β1α1. Moreover, the residues Ser211 and Asn220 on loop B are proposed as the hinge residues that are responsible for conformational alteration while the cofactor binds. The open conformation of loop B is proposed to involve Phe216 pointing out from the cofactor-binding site and the opening of the safety belt. Structural comparison with other AKRs in subfamily 4C emphasizes the role of the substrate-channel wall, consisting of Trp24, Trp115, Tyr206, Phe216, Leu291 and Phe295, in substrate discrimination. In particular, Leu291 could contribute greatly to substrate selectivity, explaining the preference of AKR4C14 for its straight-chain aldehyde substrate.





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Spectacular June 7 solar flare seen through the Atmospheric Imaging Assembly aboard Solar Dynamics Observatory

On June 7 the Sun unleashed an spectacular solar flare with a substantial coronal mass ejection. A large cloud of plasma mushroomed up, and while some parts fell back into the Sun, most rushed off into space. The first two segments of this video are seen through the Atmospheric Imaging Assembly aboard NASA's Solar Dynamics Observatory. The AIA was developed by Smithsonian scientists.

The post Spectacular June 7 solar flare seen through the Atmospheric Imaging Assembly aboard Solar Dynamics Observatory appeared first on Smithsonian Insider.




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Sweet life: tropical plants attract ants with sugary nectar

Scientific inspiration springs from many sources. In the case of Smithsonian botanist David Kenfack, ant bites were the inspiration for a recent paper he co-authored […]

The post Sweet life: tropical plants attract ants with sugary nectar appeared first on Smithsonian Insider.




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Forthcoming article in Acta Crystallographica Section E Crystallographic Communications






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Open-access and free articles in Acta Crystallographica Section C: Crystal Structural Communications




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Forthcoming article in Acta Crystallographica Section A Foundations and Advances




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New Report Calls for Eliminating Correctable and Avoidable Vision Impairments by 2030

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Global Grand Challenges Summit 2019 Will Bring Over 900 Engineers to London to Address Engineering in an Unpredictable World

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New Report Finds K-12 Teachers Face New Expectations and More Demands - Training and Workforce Changes Could Help

A new report from the National Academies of Sciences, Engineering, and Medicine finds K-12 teachers face new expectations and more demands from policymakers, parents, students, and schools, including addressing changes in curriculum standards, the emergence of more explicit teaching goals, and shifts in what it means to support all students in their development.




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Kids' Climate Case 'Reluctantly' Dismissed By Appeals Court

Levi Draheim, 11, wears a dust mask as he participates in a demonstration in Miami in July 2019. A lawsuit file by him and other young people urging action against climate change was thrown out by a federal appeals court Friday.; Credit: Wilfredo Lee/AP

Nathan Rott | NPR

A federal appeals court has dismissed a lawsuit brought by nearly two dozen young people aimed at forcing the federal government to take bolder action on climate change, saying the courts were not the appropriate place to address the issue.

A three-judge panel of the 9th U.S. Circuit Court of Appeals said Friday the young plaintiffs had "made a compelling case that action is needed," but they did not have legal standing to bring the case.

The lawsuit, Juliana v. United States, was filed in 2015 on behalf of a group of children and teenagers who said the U.S. government continued to use and promote the use of fossil fuels, knowing that such consumption would destabilize the climate, putting future generations at risk.

By doing so, the plaintiffs argued, the U.S. government had violated their constitutional rights to life, liberty and property.

Judge Andrew D. Hurwitz agreed with some of that assertion, writing in a 32-page opinion that "the federal government has long promoted fossil fuel use despite knowing that it can cause catastrophic climate change."

But, he continued, it was unclear if the court could compel the federal government to phase out fossil fuel emissions and draw down excess greenhouse gas emissions as the plaintiffs requested.

"Reluctantly, we conclude that such relief is beyond our constitutional power," Hurwitz wrote, "Rather, the plaintiffs' impressive case for redress must be presented to the political branches of government."

The decision reversed an earlier ruling by a district court judge that would have allowed the case to move forward.

Philip Gregory, who served as co-counsel for the plaintiffs, strongly disagreed with the 2-1 ruling, saying in an interview with NPR that they would seek an "en banc petition," which would put the issue before the full 9th Circuit for review.

Gregory, who spoke to some of the young plaintiffs following the decisions, says they were hopeful that their pending petition will be considered, "because as we all know, this Congress and this President will do nothing to ameliorate the climate crisis."

Both the Trump and Obama administrations opposed the lawsuit. All three of the judges involved in Friday's ruling were appointed under Obama.

Hurwitz and Judge Mary Murguia made up the majority but the third, Judge Josephine L. Staton, wrote a blistering dissent.

"In these proceedings, the government accepts as fact that the United States has reached a tipping point crying out for a concerted response — yet presses ahead toward calamity," she wrote. "It is as if an asteroid were barreling toward Earth and the government decided to shut down our only defenses."

Copyright 2020 NPR. To see more, visit https://www.npr.org.

This content is from Southern California Public Radio. View the original story at SCPR.org.




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Climate change impacts not yet detectable in river flow data




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How long do you want to live? Your expectations for old age matter

Why do some people want to live a very long time, while others would prefer to die relatively young? In a latest study, a team of researchers including Vegard Skirbekk, PhD, at the Robert N. Butler Columbia Aging Center, investigated how long young and middle-aged adults in the United States say they want to live in relation to a number of personal characteristics. The results showed that more than one out of six people would prefer to die younger than age 80, before reaching average life expectancy. There was no indication that the relationship between preferring a life shorter or longer than average life expectancy depended on age, gender or education.

read more



  • Psychology & Sociology

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<new>How predictable is the biological response to chemical toxicity?

To assess the impact on ecosystems of chemical contamination in aquatic environments, scientists need to measure not only the concentration of the contaminants, but also the extent to which they can disrupt biological processes at a cellular level in plants and animals and at an ecosystem level. According to a new study, considering these complex inter-relationships in combination will help improve the ecological status of waterways, in line with Water Framework Directive (WFD) commitments.




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Bees actively prefer nectar contaminated with neonicotinoid pesticides

Honeybees and bumblebees prefer feeding on nectar laced with certain neonicotinoid pesticides to uncontaminated food, new research has shown. Far from the predictions of some, that bees would avoid food contaminated with neonicotinoid pesticides if given the choice, a new study has shown that bees did not avoid any of the three most common neonicotinoids: imidacloprid, thiamethoxam or clothianidin. Furthermore, they showed a preference for imidacloprid and thiamethoxam over uncontaminated sugar solutions.




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Policy, not market alone, must dictate future transport emissions

Existing technologies could reduce emissions of CO2 and air pollutants from land transport by almost a third. But, reductions will not be delivered through markets alone, according to a recent assessment, particularly for CO2. The researchers argue that strong policy interventions will be essential to mitigating climate change caused by emissions from land transport.




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Increasing energy efficiency in the home may boost life expectancy and health

Changes in the home that increase energy efficiency, such as improved insulation and ventilation control, have the potential to reduce indoor air pollution. This study assessed the health impact of interventions in the UK arising from changes to indoor concentrations of fine particulate matter and found that such changes could improve health and increase life expectancy for men and women by three and two months, respectively.




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Effects of air pollution on Mediterranean plants could be studied with reflectance spectroscopy

A technique called reflectance spectroscopy is the subject of a new literature review focusing on the use of this tool to study the effects of air pollution on vegetation. In particular, the researchers suggest that the technique could be more widely applied in the Mediterranean region, to study the effects of climate change and air pollution, which will be detrimental to crop growth as well as other vegetation. It could also be used as a more general biomonitoring technique for assessing pollutant levels in the environment.




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Trainline upgrades revenue expectations as sales steam ahead





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Eastern Pacific Post-Tropical Cyclone Octave Discussion Number 9


000
WTPZ43 KNHC 192039
TCDEP3

Post-Tropical Cyclone Octave Discussion Number 9
NWS National Hurricane Center Miami FL EP182019
200 PM PDT Sat Oct 19 2019

Deep convection has not returned, now being absent for at least 12
hours. The environment around Octave is dry and stable, and it is
unlikely that significant convection will come back any time soon.
Octave has therefore become a remnant low, and its maximum winds are
estimated to be 30 kt based on recent scatterometer data. The
remnant low is expected to linger for at least the next 5 days with
its intensity holding steady or decreasing due to moderate
southeasterly shear and mid-level relative humidities of 30-40
percent.

The initial motion remains very slowly east-northeastward, or 070/2
kt. The remnant low is trapped in a weak steering regime, and it
is expected to meander for the next 5 days, only moving a net
distance of 50-100 n mi during that period. A slight westward
adjustment was made to this last NHC official forecast to account
for the latest multi-model consensus aids and the ECMWF, which lies
along the western edge of the guidance envelope.


FORECAST POSITIONS AND MAX WINDS

INIT 19/2100Z 11.6N 125.7W 30 KT 35 MPH...POST-TROPICAL
12H 20/0600Z 11.4N 125.7W 25 KT 30 MPH...POST-TROP/REMNT LOW
24H 20/1800Z 11.1N 125.7W 25 KT 30 MPH...POST-TROP/REMNT LOW
36H 21/0600Z 10.8N 125.7W 25 KT 30 MPH...POST-TROP/REMNT LOW
48H 21/1800Z 10.9N 125.7W 25 KT 30 MPH...POST-TROP/REMNT LOW
72H 22/1800Z 11.7N 125.8W 25 KT 30 MPH...POST-TROP/REMNT LOW
96H 23/1800Z 12.4N 125.4W 20 KT 25 MPH...POST-TROP/REMNT LOW
120H 24/1800Z 12.0N 124.6W 20 KT 25 MPH...POST-TROP/REMNT LOW

$$
Forecaster Berg




cta

Eastern Pacific Post-Tropical Cyclone OCTAVE Forecast/Advisory Number 9 NWS NATIONAL Hurricane CENTER MIAMI FL EP182019 2100 UTC SAT OCT 19 2019 THERE ARE NO COASTAL WATCHES OR WARNINGS IN EFFECT. POST-TROPICAL Cyclone CENTER LOCATED NEAR 11.6N 125.7W AT


000
WTPZ23 KNHC 192037
TCMEP3

POST-TROPICAL CYCLONE OCTAVE FORECAST/ADVISORY NUMBER 9
NWS NATIONAL HURRICANE CENTER MIAMI FL EP182019
2100 UTC SAT OCT 19 2019

THERE ARE NO COASTAL WATCHES OR WARNINGS IN EFFECT.

POST-TROPICAL CYCLONE CENTER LOCATED NEAR 11.6N 125.7W AT 19/2100Z
POSITION ACCURATE WITHIN 15 NM

PRESENT MOVEMENT TOWARD THE EAST-NORTHEAST OR 70 DEGREES AT 2 KT

ESTIMATED MINIMUM CENTRAL PRESSURE 1009 MB
MAX SUSTAINED WINDS 30 KT WITH GUSTS TO 35 KT.
WINDS AND SEAS VARY GREATLY IN EACH QUADRANT. RADII IN NAUTICAL
MILES ARE THE LARGEST RADII EXPECTED ANYWHERE IN THAT QUADRANT.

REPEAT...CENTER LOCATED NEAR 11.6N 125.7W AT 19/2100Z
AT 19/1800Z CENTER WAS LOCATED NEAR 11.6N 125.8W

FORECAST VALID 20/0600Z 11.4N 125.7W...POST-TROP/REMNT LOW
MAX WIND 25 KT...GUSTS 35 KT.

FORECAST VALID 20/1800Z 11.1N 125.7W...POST-TROP/REMNT LOW
MAX WIND 25 KT...GUSTS 35 KT.

FORECAST VALID 21/0600Z 10.8N 125.7W...POST-TROP/REMNT LOW
MAX WIND 25 KT...GUSTS 35 KT.

FORECAST VALID 21/1800Z 10.9N 125.7W...POST-TROP/REMNT LOW
MAX WIND 25 KT...GUSTS 35 KT.

FORECAST VALID 22/1800Z 11.7N 125.8W...POST-TROP/REMNT LOW
MAX WIND 25 KT...GUSTS 35 KT.

EXTENDED OUTLOOK. NOTE...ERRORS FOR TRACK HAVE AVERAGED NEAR 100 NM
ON DAY 4 AND 150 NM ON DAY 5...AND FOR INTENSITY NEAR 15 KT EACH DAY

OUTLOOK VALID 23/1800Z 12.4N 125.4W...POST-TROP/REMNT LOW
MAX WIND 20 KT...GUSTS 30 KT.

OUTLOOK VALID 24/1800Z 12.0N 124.6W...POST-TROP/REMNT LOW
MAX WIND 20 KT...GUSTS 30 KT.

REQUEST FOR 3 HOURLY SHIP REPORTS WITHIN 300 MILES OF 11.6N 125.7W

THIS IS THE LAST FORECAST/ADVISORY ISSUED BY THE
NATIONAL HURRICANE CENTER ON THIS SYSTEM

$$
FORECASTER BERG





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Eastern Pacific Post-Tropical Cyclone Octave Advisory Number 9


000
WTPZ33 KNHC 192038
TCPEP3

BULLETIN
Post-Tropical Cyclone Octave Advisory Number 9
NWS National Hurricane Center Miami FL EP182019
200 PM PDT Sat Oct 19 2019

...OCTAVE BECOMES A REMNANT LOW...
...THIS IS THE LAST ADVISORY...


SUMMARY OF 200 PM PDT...2100 UTC...INFORMATION
----------------------------------------------
LOCATION...11.6N 125.7W
ABOUT 1300 MI...2090 KM SW OF THE SOUTHERN TIP OF BAJA CALIFORNIA
MAXIMUM SUSTAINED WINDS...35 MPH...55 KM/H
PRESENT MOVEMENT...ENE OR 70 DEGREES AT 2 MPH...4 KM/H
MINIMUM CENTRAL PRESSURE...1009 MB...29.80 INCHES


WATCHES AND WARNINGS
--------------------
There are no coastal watches or warnings in effect.


DISCUSSION AND OUTLOOK
----------------------
At 200 PM PDT (2100 UTC), the center of Post-Tropical Cyclone Octave
was located near latitude 11.6 North, longitude 125.7 West. Octave
is moving toward the east-northeast near 2 mph. A slow and erratic
motion is expected during the next few days.

Maximum sustained winds are near 35 mph (55 km/h) with higher gusts.
Little change in strength is forecast during the next few days.

The estimated minimum central pressure is 1009 mb (29.80 inches).


HAZARDS AFFECTING LAND
----------------------
None


NEXT ADVISORY
-------------
This is the last public advisory issued by the National Hurricane
Center on this system. For additional information on the remnant low
please see High Seas Forecasts issued by the National Weather
Service, under AWIPS header NFDHSFEPI, WMO header FZPN02 KWBC, and
on the web at ocean.weather.gov/shtml/NFDHSFEPI.php

$$
Forecaster Berg




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Eastern Pacific Post-Tropical Cyclone OCTAVE ICAO Advisory Number 9 NWS NATIONAL Hurricane CENTER MIAMI FL EP182019 2100 UTC SAT OCT 19 2019 TC ADVISORY DTG: 20191019/2100Z TCAC: KNHC TC: OCTAVE NR: 009 PSN: N1136 W12542 MOV: ENE 02KT C: 1009HPA MAX WIND:

 
 000
 FKPZ23 KNHC 192039
 TCAPZ3
 
 POST-TROPICAL CYCLONE OCTAVE ICAO ADVISORY NUMBER   9
 NWS NATIONAL HURRICANE CENTER MIAMI FL       EP182019
 2100 UTC SAT OCT 19 2019
 
 TC ADVISORY
 DTG:                      20191019/2100Z
 TCAC:                     KNHC
 TC:                       OCTAVE
 NR:                       009
 PSN:                      N1136 W12542
 MOV:                      ENE 02KT
 C:                        1009HPA
 MAX WIND:                 030KT
 FCST PSN +6 HR:           20/0300Z N1127 W12542
 FCST MAX WIND +6 HR:      025KT
 FCST PSN +12 HR:          20/0900Z N1119 W12542
 FCST MAX WIND +12 HR:     025KT
 FCST PSN +18 HR:          20/1500Z N1110 W12542
 FCST MAX WIND +18 HR:     025KT
 FCST PSN +24 HR:          20/2100Z N1101 W12542
 FCST MAX WIND +24 HR:     025KT
 RMK:                      THE FORECAST POSITION INFORMATION IN
                           THIS PRODUCT IS INTERPOLATED FROM
                           OFFICIAL FORECAST DATA VALID AT 0000...
                           0600...1200...AND 1800Z.
 NXT MSG:                  NO MSG EXP
 
 ..
 
 




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Eastern Pacific Post-Tropical Cyclone OCTAVE Wind Speed Probabilities Number 9 NWS NATIONAL Hurricane CENTER MIAMI FL EP182019 2100 UTC SAT OCT 19 2019 AT 2100Z THE CENTER OF POST-TROPICAL Cyclone OCTAVE WAS LOCATED NEAR LATITUDE 11.6 NORTH...LONGITUDE 12

 
 000
 FOPZ13 KNHC 192039
 PWSEP3
                                                                     
 POST-TROPICAL CYCLONE OCTAVE WIND SPEED PROBABILITIES NUMBER   9    
 NWS NATIONAL HURRICANE CENTER MIAMI FL       EP182019               
 2100 UTC SAT OCT 19 2019                                            
                                                                     
 AT 2100Z THE CENTER OF POST-TROPICAL CYCLONE OCTAVE WAS LOCATED NEAR
 LATITUDE 11.6 NORTH...LONGITUDE 125.7 WEST WITH MAXIMUM SUSTAINED   
 WINDS NEAR 30 KTS...35 MPH...55 KM/H.                               
                                                                     
 Z INDICATES COORDINATED UNIVERSAL TIME (GREENWICH)                  
    PACIFIC  DAYLIGHT TIME (PDT)...SUBTRACT 7 HOURS FROM Z TIME      
    HAWAIIAN STANDARD TIME (HST)...SUBTRACT 10 HOURS FROM Z TIME     
                                                                     
 WIND SPEED PROBABILITY TABLE FOR SPECIFIC LOCATIONS                 
                                                                     
 CHANCES OF SUSTAINED (1-MINUTE AVERAGE) WIND SPEEDS OF AT LEAST     
    ...34 KT (39 MPH... 63 KM/H)...                                  
    ...50 KT (58 MPH... 93 KM/H)...                                  
    ...64 KT (74 MPH...119 KM/H)...                                  
 FOR LOCATIONS AND TIME PERIODS DURING THE NEXT 5 DAYS               
                                                                     
 PROBABILITIES FOR LOCATIONS ARE GIVEN AS OP(CP) WHERE               
     OP  IS THE PROBABILITY OF THE EVENT BEGINNING DURING            
         AN INDIVIDUAL TIME PERIOD (ONSET PROBABILITY)               
    (CP) IS THE PROBABILITY OF THE EVENT OCCURRING BETWEEN           
         18Z SAT AND THE FORECAST HOUR (CUMULATIVE PROBABILITY)      
                                                                     
 PROBABILITIES ARE GIVEN IN PERCENT                                  
 X INDICATES PROBABILITIES LESS THAN 1 PERCENT                       
 PROBABILITIES FOR 34 KT AND 50 KT ARE SHOWN AT A GIVEN LOCATION WHEN
 THE 5-DAY CUMULATIVE PROBABILITY IS AT LEAST 3 PERCENT.             
 PROBABILITIES FOR 34...50...64 KT SHOWN WHEN THE 5-DAY              
 64-KT CUMULATIVE PROBABILITY IS AT LEAST 1 PERCENT.                 
                                                                     
                                                                     
   - - - - WIND SPEED PROBABILITIES FOR SELECTED LOCATIONS - - - -   
                                                                     
                FROM    FROM    FROM    FROM    FROM    FROM    FROM 
   TIME       18Z SAT 06Z SUN 18Z SUN 06Z MON 18Z MON 18Z TUE 18Z WED
 PERIODS         TO      TO      TO      TO      TO      TO      TO  
              06Z SUN 18Z SUN 06Z MON 18Z MON 18Z TUE 18Z WED 18Z THU
                                                                     
 FORECAST HOUR    (12)   (24)    (36)    (48)    (72)    (96)   (120)
 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 
 LOCATION       KT                                                   
                                                                     
 10N 125W       34  1   2( 3)   4( 7)   7(14)   1(15)   1(16)   2(18)
  
 15N 125W       34  X   X( X)   X( X)   1( 1)   1( 2)   1( 3)   2( 5)
  
 $$                                                                  
 FORECASTER BERG                                                     
 




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&#39;Injectable bandage&#39; stops the bleeding with an assist from seaweed

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Spectacular splintering of Perito Moreno glacier caught on film

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These stone fruits look and taste similar, but they're not always interchangeable in recipes.




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U.S. infant death rate down, life expectancy steady

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What is nectar dearth?

When flowers start to dry up and die, bees struggle to find nectar and pollen. Here are some signs of nectar death and what you can do to help.



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Spectacular things happen when you let winter do your hair

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