The BMW M8 Coupe is the flagship offering from the German auto giant in India and promises a blend of luxury with dynamic performance via a 4.4-litre V8 motor.

Participants in Delaware's Women, Infants and Children (WIC) program can now use special coupons to purchase fresh fruits and vegetables at 11 Delaware farmers' markets under a pilot program beginning this week.

 Prudential norms on Investment in Zero Coupon Bonds

BMW 8 Series line-up will be positioned above the 6 Series Gran Coupe and will come in two variants - the 840i Gran Coupe and the M8 Coupe.

Ishant Sharma travelled for 24 hours and arrived in New Zealand 72 hours before the start of the first Test at Wellington.

Market participants have been anticipating the coupon on the new 10-year bonds to settle at levels close to 5.85%, around 15-18 basis points lower than the yield on the current benchmark bonds.

2020 Hyundai Elantra vs. 2020 Toyota Corolla: Compare Cars The 2020 Toyota Corolla and 2020 Hyundai Elantra are two compact cars—and hatchbacks—that are great values with thrifty hybrids. Mailbag: What's the difference between car, crossover, and SUV anyway? "Why isn't an SUV a station wagon or a crossover?" a perplexed reader wrote...

BMW has been spotted testing what appears to be a new performance flagship for the M5 range. Word on the street is that the car, which will likely be dubbed an M5 CS, is powered by a newly developed V-8. Porsche looks to be readying a Cayenne GTS Coupe to help bridge the performance gap between the S and Turbo. The new variant should debut next...

We spent some time in the 2020 BMW 8-Series Gran Coupe; we drove the 2020 Genesis G70; and coronavirus has affected the 2020 Chevrolet Corvette. It's the Week in Reverse, right here at Motor Authority. The 2020 BMW 8-Series Gran Coupe is a more stylish four-door alternative to the 7-Series. We found it has a Teutonic rumble from its twin-turbo...

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U.S. President, Donald Trump, on Friday, said he would send an army to topple his Venezuelan counterpart, Nicolás Maduro if he wanted to. Trump said this while speaking on Fox and Friends, a live talk show on Fox Television as he denied the U.S. involvement in an alleged recent coup attempt on Maduro. “We have […]

Trump clears air on alleged coup to overthrow Venezuela leader

A government study said it had injected a total of 168.7 trillion won (US$150.8 billion) since the 1997 Asian financial crisis as part of an effort to clean up insolvent financial services firms and has only recouped 115 trillion won as of the end of the second quarter of this year. This is the recovery ratio of 68.3 percent in 20 years' time. Even though it looks good in appearance, there is a big difference from that of the U.S. government. The U.S. government had spent $426.4 billion for...

Police are investigating a house robbery in which a couple in Amanzimtoti, on the south coast of KwaZulu-Natal, believed to be in their 50s was stabbed. ......

A married couple from Maryland ages 85 and 86 were the victims of a deadly shooting at a veterans cemetery in Delaware.

The Venezuelan military defector...

Calls for Australian businesses to diversify away from China as their main trading partner are nothing more than a “zombie economic idea” that is being used as a political tool to expedite the decoupling of the two countries, according to a report by the Australia-China Relations Institute at the University of Technology Sydney.The debate about Australia’s over-dependence on China for its livelihood has ramped up recently, triggered by the interruption of supply chains during China’s…

They had visited more than 20 countries before coronavirus struck.

An analysis of the topics that cause arguments between newly-wed heterosexual couples puts a lack of affection at the top of the list, with little concern about who sleeps on which side of the bed

Title: What Couples Considering IVF Need to Know
Category: Health News
Created: 5/1/2019 12:00:00 AM
Last Editorial Review: 5/2/2019 12:00:00 AM

Title: Love in the Time of Coronavirus: Couples Feel the Strain of Lockdown
Category: Health News
Created: 4/28/2020 12:00:00 AM
Last Editorial Review: 4/29/2020 12:00:00 AM

ABSTRACT

Cell division requires proper spatial coordination with the chromosome, which undergoes dynamic changes during chromosome replication and segregation. FtsZ is a bacterial cytoskeletal protein that assembles into the Z-ring, providing a platform to build the cell division apparatus. In the model bacterium Caulobacter crescentus, the cellular localization of the Z-ring is controlled during the cell cycle in a chromosome replication-coupled manner. Although dynamic localization of the Z-ring at midcell is driven primarily by the replication origin-associated FtsZ inhibitor MipZ, the mechanism ensuring accurate positioning of the Z-ring remains unclear. In this study, we showed that the Z-ring colocalizes with the replication terminus region, located opposite the origin, throughout most of the C. crescentus cell cycle. Spatial organization of the two is mediated by ZapT, a previously uncharacterized protein that interacts with the terminus region and associates with ZapA and ZauP, both of which are part of the incipient division apparatus. While the Z-ring and the terminus region coincided with the presence of ZapT, colocalization of the two was perturbed in cells lacking zapT, which is accompanied by delayed midcellular positioning of the Z-ring. Moreover, cells overexpressing ZapT showed compromised positioning of the Z-ring and MipZ. These findings underscore the important role of ZapT in controlling cell division processes. We propose that ZapT acts as a molecular bridge that physically links the terminus region to the Z-ring, thereby ensuring accurate site selection for the Z-ring. Because ZapT is conserved in proteobacteria, these findings may define a general mechanism coordinating cell division with chromosome organization.

IMPORTANCE Growing bacteria require careful tuning of cell division processes with dynamic organization of replicating chromosomes. In enteric bacteria, ZapA associates with the cytoskeletal Z-ring and establishes a physical linkage to the chromosomal replication terminus through its interaction with ZapB-MatP-DNA complexes. However, because ZapB and MatP are found only in enteric bacteria, it remains unclear how the Z-ring and the terminus are coordinated in the vast majority of bacteria. Here, we provide evidence that a novel conserved protein, termed ZapT, mediates colocalization of the Z-ring with the terminus in Caulobacter crescentus, a model organism that is phylogenetically distant from enteric bacteria. Given that ZapT facilitates cell division processes in C. crescentus, this study highlights the universal importance of the physical linkage between the Z-ring and the terminus in maintaining cell integrity.

In order to establish sustainable heat loading (heat removal and storage) in abandoned flooded mine workings it is important to understand the geomechanical impact of the cyclical heat loading caused by fluid injection and extraction. This is particularly important where significantly more thermal loading is planned than naturally occurs. A simple calculation shows that the sustainable geothermal heat flux from abandoned coal mines can provide less than a tenth of Scotland's annual domestic heating demand. Any heat removal greater than the natural heat flux will lead to heat mining unless heat storage options are also considered.

As a first step, a steady-state, fully saturated, 2D coupled hydromechanical model of a generalized section of pillar-and-stall workings has been created. Mine water rebound was modelled by increasing the hydrostatic pressure sequentially, in line with monitored mine water-level data from Midlothian, Scotland. The modelled uplift to water-level rise ratio of 1.4 mm m^–1 is of the same order of magnitude (1 mm m^–1) as that observed through interferometric synthetic aperture radar (InSAR) data in the coalfield due to mine water rebound. The modelled magnitude of shear stress at the pillar corners, as a result of horizontal and vertical displacement, is shown to increase linearly with water level. Mine heat systems are expected to cause smaller changes in pressure than those modelled but the results provide initial implications on the potential geomechanical impacts of mine water heat schemes which abstract or inject water and heat into pillar-and-stall coal mine workings.

Thematic collection: This article is part of the SJG Collection on Early-Career Research available at: https://www.lyellcollection.org/cc/SJG-early-career-research

Many noncoding RNAs are known to play a role in the cell directly linked to their structure. Structure prediction based on the sole sequence is, however, a challenging task. On the other hand, thanks to the low cost of sequencing technologies, a very large number of homologous sequences are becoming available for many RNA families. In the protein community, the idea of exploiting the covariance of mutations within a family to predict the protein structure using the direct-coupling-analysis (DCA) method has emerged in the last decade. The application of DCA to RNA systems has been limited so far. We here perform an assessment of the DCA method on 17 riboswitch families, comparing it with the commonly used mutual information analysis and with state-of-the-art R-scape covariance method. We also compare different flavors of DCA, including mean-field, pseudolikelihood, and a proposed stochastic procedure (Boltzmann learning) for solving exactly the DCA inverse problem. Boltzmann learning outperforms the other methods in predicting contacts observed in high-resolution crystal structures.

Allostery exploits the conformational dynamics of enzymes by triggering a shift in population ensembles toward functionally distinct conformational or dynamic states. Allostery extensively regulates the activities of key enzymes within biosynthetic pathways to meet metabolic demand for their end products. Here, we have examined a critical enzyme, 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase (DAH7PS), at the gateway to aromatic amino acid biosynthesis in Mycobacterium tuberculosis, which shows extremely complex dynamic allostery: three distinct aromatic amino acids jointly communicate occupancy to the active site via subtle changes in dynamics, enabling exquisite fine-tuning of delivery of these essential metabolites. Furthermore, this allosteric mechanism is co-opted by pathway branchpoint enzyme chorismate mutase upon complex formation. In this study, using statistical coupling analysis, site-directed mutagenesis, isothermal calorimetry, small-angle X-ray scattering, and X-ray crystallography analyses, we have pinpointed a critical node within the complex dynamic communication network responsible for this sophisticated allosteric machinery. Through a facile Gly to Pro substitution, we have altered backbone dynamics, completely severing the allosteric signal yet remarkably, generating a nonallosteric enzyme that retains full catalytic activity. We also identified a second residue of prime importance to the inter-enzyme communication with chorismate mutase. Our results reveal that highly complex dynamic allostery is surprisingly vulnerable and provide further insights into the intimate link between catalysis and allostery.

Chalongrat Noree and Naraporn Sirinonthanawech

Previously, we have developed an extramitochondrial assembly system, where mitochondrial targeting signal (MTS) can be removed from a given mitochondrial enzyme, which could be used to characterize the regulatory factors involved in enzyme assembly/disassembly in vivo. Here, we demonstrate that addition of exogenous acetaldehyde can quickly induce the supramolecular assembly of MTS-deleted aldehyde dehydrogenase Ald4p in yeast cytoplasm. Also, by using PCR-based modification of the yeast genome, cytoplasmically targeted Ald4p cannot polymerize into long filaments when key functional amino acid residues are substituted, as shown by N192D, S269A, E290K and C324A mutations. This study has confirmed that extramitochondrial assembly could be a powerful external system for studying mitochondrial enzyme assembly, and its regulatory factors outside the mitochondria. In addition, we propose that mitochondrial enzyme assembly/disassembly is coupled to the regulation of a given mitochondrial enzyme activity.

Burkholderia sp. strain SG-MS1 and Pseudomonas sp. strain SG-MS2 have previously been found to mineralize (+)-pinoresinol through a common catabolic pathway. Here, we used comparative genomics, proteomics, protein semipurification, and heterologous expression to identify a flavoprotein from the vanillyl alcohol oxidase/p-cresol methyl hydroxylase (VAO/PCMH) enzyme family in SG-MS2 that carries out the initial hydroxylation of (+)-pinoresinol at the benzylic carbon. The cognate gene is translationally coupled with a downstream cytochrome gene, and the cytochrome is required for activity. The flavoprotein has a unique combination of cofactor binding and cytochrome requirements for the VAO/PCMH family. The heterologously expressed enzyme has a K_m of 1.17 μM for (+)-pinoresinol. The enzyme is overexpressed in strain SG-MS2 upon exposure to (+)-pinoresinol, along with 45 other proteins, 22 of which were found to be encoded by genes in an approximately 35.1-kb cluster also containing the flavoprotein and cytochrome genes. Homologs of 18 of these 22 genes, plus the flavoprotein and cytochrome genes, were also found in a 38.7-kb cluster in SG-MS1. The amino acid identities of four of the other proteins within the SG-MS2 cluster suggest they catalyze conversion of hydroxylated pinoresinol to protocatechuate and 2-methoxyhydroquinone. Nine other proteins upregulated in SG-MS2 on exposure to (+)-pinoresinol appear to be homologs of proteins known to comprise the protocatechuate and 2-methoxyhydroquinone catabolic pathways, but only three of the cognate genes lie within the cluster containing the flavoprotein and cytochrome genes.

IMPORTANCE (+)-Pinoresinol is an important plant defense compound, a major food lignan for humans and some other animals, and the model compound used to study degradation of the β-β' linkages in lignin. We report a gene cluster, in one strain each of Pseudomonas and Burkholderia, that is involved in the oxidative catabolism of (+)-pinoresinol. The flavoprotein component of the α-hydroxylase which heads the pathway belongs to the 4-phenol oxidizing (4PO) subgroup of the vanillyl alcohol oxidase/p-cresol methyl hydroxylase (VAO/PCMH) enzyme family but constitutes a novel combination of cofactor and electron acceptor properties for the family. It is translationally coupled with a cytochrome gene whose product is also required for activity. The work casts new light on the biology of (+)-pinoresinol and its transformation to other bioactive molecules. Potential applications of the findings include new options for deconstructing lignin into useful chemicals and the generation of new phytoestrogenic enterolactones from lignans.

To make the dream of settling down in a prosperous city come true, young couples, especially ones with limited budgets, need to prepare the right steps to get your home sweet home.

लग्जरी कार बनाने वाली जर्मनी की दिग्गज ऑटोमोबाइल कंपनी BMW (बीएमडब्ल्यू) ने अपनी BMW 8 Series Gran Coupe (8-सीरीज ग्रैन कूपे) और BMW M8 Coupe (एम-8 कूपे) भारतीय बाजार में लॉन्च कर दिया है।

Getting married is one of life’s most memorable — and emotional — milestones in life. But with tight restrictions on gatherings due to COVID-19 pandemic, couples throughout Windsor and Essex County who had scheduled their weddings for this spring or summer have had little choice but to postpone their big day. “Weddings are an extremely […]

MP called for love to be added to list of permitted reasons for long-distance journeys

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Couples separated by France’s strict coronavirus rules will remain lovelorn after ministers ruled out a proposed change to the law extending the country’s state of health emergency.

The “lovers’ amendment”, as it was called, was proposed by an MP during a debate on the legislation in the lower house the national assembly.

For the first time, researchers have succeeded in creating strong coupling between quantum systems over a greater distance. They accomplished this with a novel method in which a laser loop connects the systems, enabling nearly lossless exchange of information and strong interaction between them. The physicists reported that the new method opens up new possibilities in quantum networks and quantum sensor technology.

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A couple have been fined after getting stranded on a rock on the Scottish coast during the coronavirus lockdown.

A couple who were living their dream of sailing across the Atlantic have shared their "shock" of returning to land and hearing the news about the global coronavirus pandemic.

Flamingos form long-lasting friendships and some "behave like married couples", according to new research.

A couple spending their diamond wedding anniversary apart because of the coronavirus pandemic each got a special treat to make their celebrations a little sweeter thanks to their local supermarket.

Tributes have been paid to Ken and Betty Hill, who were together for more than 40 years

An Australian couple has discovered a novel way to meet up with neighbours and enjoy the good weather, despite the coronavirus lockdown.

A new biography of Harry and Meghan will "go beyond the headlines" and "reveal unknown details" of the couple's life together.

Auto-pay compounded Comcast error, leading to a year of $50 monthly overcharges.

'We've never been apart for sixty plus years, we don't do apart,' says Sidney Moore

Netflix's new dating series ended with a polarising finale, and one that also left a number of lingering questions about the show's two central couples

Cornwall was considered as a new location for the 2020 series

Has your wedding been affected by coronavirus? Deliveroo wants to help

Thanks to Zoom, 50 of their friends were able to attend.

For the first time, researchers have succeeded in creating strong coupling between quantum systems over a greater distance. They accomplished this with a novel method in which a laser loop connects the systems, enabling nearly lossless exchange of information and strong interaction between them. In the scientific journal Science, the physicists from the University of Basel and University of Hanover reported that the new method opens up new possibilities in quantum networks and quantum sensor technology.