(University of California - Santa Cruz) Restoration ecologist Karen Holl has a simple message for anyone who thinks planting 1 trillion trees will reverse the damage of climate change: 'We can't plant our way out of climate change.'

The respiratory epithelium comprises polarized cells at the interface between the environment and airway tissues. Polarized apical and basolateral protein secretions are a feature of airway epithelium homeostasis. Human respiratory syncytial virus (hRSV) is a major human pathogen that primarily targets the respiratory epithelium. However, the consequences of hRSV infection on epithelium secretome polarity and content remain poorly understood. To investigate the hRSV-associated apical and basolateral secretomes, a proteomics approach was combined with an ex vivo pediatric human airway epithelial (HAE) model of hRSV infection (data are available via ProteomeXchange and can be accessed at https://www.ebi.ac.uk/pride/ with identifier PXD013661). Following infection, a skewing of apical/basolateral abundance ratios was identified for several individual proteins. Novel modulators of neutrophil and lymphocyte activation (CXCL6, CSF3, SECTM1 or CXCL16), and antiviral proteins (BST2 or CEACAM1) were detected in infected, but not in uninfected cultures. Importantly, CXCL6, CXCL16, CSF3 were also detected in nasopharyngeal aspirates (NPA) from hRSV-infected infants but not healthy controls. Furthermore, the antiviral activity of CEACAM1 against RSV was confirmed in vitro using BEAS-2B cells. hRSV infection disrupted the polarity of the pediatric respiratory epithelial secretome and was associated with immune modulating proteins (CXCL6, CXCL16, CSF3) never linked with this virus before. In addition, the antiviral activity of CEACAM1 against hRSV had also never been previously characterized. This study, therefore, provides novel insights into RSV pathogenesis and endogenous antiviral responses in pediatric airway epithelium.

Research Event

Event participants

Dr Jon Gibbins, Senior Lecturer in the Department of Mechanical Engineering, Imperial College London
Jim Footner, Senior Climate Change Campaigner, Greenpeace

Some apps have the potential to encourage healthier habits and are accessible to most people, argues Iltifat Husain, but Des Spence notes the lack of any evidence of effectiveness and the potential for encouraging unnecessary anxiety. Read more about in our head to head "Can healthy people benefit from health apps?" -...

Providing information to enable informed choices about healthcare sounds immediately appealing to most of us. But Minna Johansson, GP trainee and PhD student at the University of Gothenburg, argues that preventive medicine and expanding disease definitions have changed the ethical premises of informed choice and our good intentions may...

Results from a University of Iowa College of Dentistry study suggest that mild and moderate dental fluorosis is generally less noticeable over time, validating the beliefs of some supporters of community water fluoridation that there have been overestimates of fluorosis prevalence made by anti-fluoridation activists.

Some 30 companies including Dow, Total and other big makers of plastic raised $1 billion to contribute to cleaner ocean waters.

The ambitious immigration overhaul package that Congress is studying has drawn criticism from conservatives who say it offers amnesty to lawbreakers, and from immigration advocates who say it will not do enough to bring millions of people out of the shadows.

200 gm flaked salt 200 gm brown sugar 1 side ocean trout, skin on, pinboned 1/2 cup smoking shavings/sawdust Salad 1 butter lettuce 1 baby endive lettuce 2 Lebanese cucumbers 200 ml creme fraiche 100 ml vinaigrette (25 ml sherry vinegar, 25 ml olive oil, 50 ml grape seed oil) Salt and pepper 2 Granny Smith apples 1 stick celery Bunch chives

The ocean is a naturally noisy place full of singing whales, grunting fish, snapping shrimp, cracking ice, wind and rain. But human-made sounds -- from ship engines to oil drilling -- have become an acute threat to marine life, says science journalist Nicola Jones. Watch (and listen) as she discusses the strange things that happen to underwater creatures in the face of ocean noise pollution -- and shares straightforward ways we can dial down the sound to see almost immediate impacts.

Fishery management -- United States -- History -- 20th century.

[Paris?] : [J. Androuet du Cerceau?], [1562?]

Today’s fisheries sector hosts a multibillion dollar industry that is a vital source of food and nutrition, employment, trade, economic wellbeing and recreation. What is blue growth? The concept of a "blue economy" came out of the 2012 Rio+20 Conference and emphasizes conservation and sustainable management, based on the premise that healthy ocean ecosystems are more productive and a must for sustainable [...]

The oceans have it all: from microscopic life to the largest animal that has ever lived on Earth, from the colourless to the shimmering, from the frozen to the boiling and from the sunlit to the mysterious dark of the deepest parts of the planet. Oceans are an essential component of the Earth's ecosystem -- a source of biodiversity, food, and [...]

It is well known that forests, especially rainforests, are key allies in our fight against climate change as they absorb greenhouse gas emissions. But did you know that oceans are the earth’s main buffer against climate change? In fact, about 25 percent of the greenhouse gases that we emit actually gets absorbed by the oceans, as does over 90 percent [...]

Ghost fishing. Sounds eerie, right? Unfortunately, it is indeed as eerie as it sounds. Ghost fishing occurs when lost or abandoned fishing gear stays in the ocean and traps fish or other marine life, indiscriminately killing whatever it catches. Abandoned, lost or otherwise discarded fishing gear (ALDFG), as it is known officially, is being recognized as a topic that we must [...]

With the health of our oceans at stake, illegal, unreported and unregulated (IUU) fishing has quickly become a monumental problem. The term “IUU fishing” is used for any fishing activities that operate outside of the law. There are many types of IUU fishing, for example, fishing without license or authorization, not accurately reporting the fish caught, fishing in prohibited areas [...]

Oceans cover 70 percent of our planet. But did you know that 40 percent of the earth’s surface is covered by what is known as our common oceans?  

‘What on earth were they doing there?’ one researcher asks

The impact that created Pluto’s 'heart' may have rippled through its ocean and damaged its rear

Researchers shared a video of this massive siphonophore, one of the longest of its kind ever recorded

The crustaceans' guts pulverize plastics into tiny bits that can be consumed by even smaller creatures at the base of the ocean food chain

Before probing blots for the presence of an antigen, the total composition of the transferred proteins can be determined by staining the nitrocellulose or polyvinylidene fluoride (PVDF) membrane. Staining for proteins is useful to determine the position of the non-prestained molecular weight markers or individual lanes on the gel and to ensure that efficient transfer has occurred. It can be also used to verify equal loading of the samples in the gel when a comparison of the protein of interest between the different samples is important. The conventional procedures such as Coomassie Blue and silver staining methods used for staining polyacrylamide gels are incompatible with immunoblotting. Ponceau S is the more common staining method in immunoblotting protocols because it is compatible with antibody–antigen binding, is cost efficient, and provides a good contrast between the stained bands and background. In this protocol, nitrocellulose or PVDF membrane is rinsed with ultrapure H₂O after the transfer of proteins. Ponceau S dye is applied as an acidic aqueous solution, and the proteins on the membrane are stained with red color. The membrane is briefly destained with water and can be photographed or scanned to obtain the image of the total protein staining. Individual lane positions or the molecular weight standards can be marked with a pencil, if required.

Officials with Maritime Pride Eggs say their Summerside, P.E.I., egg facility will cease processing operations on June 5.

Most states take a very hands-off approach to regulating home schooling, and some advocates worry that makes it attractive to neglectful and abusive parents.

The Cpx stress response is widespread among Enterobacteriaceae. We have previously reported a mutation in cpxA in a multidrug resistant strain of Klebsiella aerogenes isolated from a patient treated with imipenem. This mutation yields to a single amino acid substitution (Y144N) located in the periplasmic sensor domain of CpxA. In this work, we sought to characterize this mutation in Escherichia coli by using genetic and biochemical approaches. Here, we show that cpxA^Y144N is an activated allele that confers resistance to β-lactams and aminoglycosides in a CpxR-dependent manner, by regulating the expression of the OmpF porin and the AcrD efflux pump, respectively. We also demonstrate the intimate interconnection between Cpx system and peptidoglycan integrity on the expression of an exogenous AmpC β-lactamase by using imipenem as a cell wall active antibiotic or inactivation of penicillin-binding proteins. Moreover, our data indicate that the Y144N substitution abrogates the interaction between CpxA and CpxP and increase phosphotransfer activity on CpxR. Because the addition of a strong AmpC inducer such as imipenem is known to causes abnormal accumulation of muropeptides (disaccharide-pentapeptide, N-acetylglucosamyl-1,6-anhydro-N-acetylmuramyl-l-alanyl-d-glutamy-meso-diaminopimelic-acid-d-alanyl-d-alanine) in the periplasmic space, we propose these molecules activate the Cpx system by displacing CpxP from the sensor domain of CpxA. Altogether, these data could explain why large perturbations to peptidoglycan caused by imipenem lead to mutational activation of the Cpx system and bacterial adaptation through multidrug resistance. These results also validate the Cpx system, in particular the interaction between CpxA and CpxP, as a promising therapeutic target.

QPX7728 is an ultra-broad-spectrum boronic acid beta-lactamase inhibitor that demonstrates inhibition of key serine and metallo beta-lactamases at a nano molar range in biochemical assays with purified enzymes. The broad-spectrum inhibitory activity of QPX7728 observed in biochemical experiments translates into enhancement of the potency of many beta-lactams against strains of target pathogens producing beta-lactamases. The impact of bacterial efflux and permeability on inhibitory potency were determined using isogenic panels of KPC-3 producing isogenic strains of K. pneumoniae and P. aeruginosa and OXA-23-producing strains of A. baumannii with various combinations of efflux and porin mutations. QPX7728 was minimally affected by multi-drug resistance efflux pumps in either Enterobacteriaceae, or in non-fermenters such as P. aeruginosa or A. baumannii. In P. aeruginosa, the potency of QPX7728 was further enhanced when the outer membrane is permeabilized. The potency of QPX7728 in P. aeruginosa is not affected by inactivation of the carbapenem porin OprD. While changes in OmpK36 (but not OmpK35) reduced the potency of QPX7728 (8-16-fold), QPX7728 (4 μg/ml) nevertheless completely reversed KPC-mediated meropenem resistance in strains with porin mutations, consistent with a lesser effect of these mutations on the potency of QPX7728 compared to other agents. The ultra-broad-spectrum beta-lactamase inhibition profile combined with enhancement of the activity of multiple beta-lactam antibiotics with varying sensitivity to the intrinsic resistance mechanisms of efflux and permeability indicate QPX7728 is a useful inhibitor for use with multiple beta-lactam antibiotics.

The hysteria about FaceApp privacy is mostly overblown, but the app does some shady things that many other apps do, too. Senior Security Analyst Max Eddy examines whether deleting apps like FaceApp can restore your privacy.

From the ashes of the failed Fisker Karma sports car, automotive designer Henrik Fisker has designed a futuristic battery-powered luxury SUV that won't break the bank.

Warranties expiring between March 1, 2020 and May 31, 2020 have now been extended for a period of 3 months.

Delaware's Division of Public Health (DPH) is advising Ocean View residents who reside in the Plantation Park community of a positive case of rabies in a feral kitten that came into contact with a human.

Ocean View --

Location: West Avenue to Central Avenue and Atlantic Avenue, Ocean View (Stingray Harbor Subdivision).

Times and Dates: 7:00 a.m. until 5:00 p.m. on Tuesday, March 17, 2020, pending weather.

Rain Date: Wednesday, March 18, 2020 starting at 7:00 a.m. until 5:00 p.m. [More]