rediffGURU Patrick Dsouza, founder of Patrick100 offers advice on how to optimise your preparation for CAT 2025.

Mutual funds (MFs) reinforced their record monthly inflows in October with an investment of Rs 87,000 crore (up to October 29), softening the downside pressure on domestic markets. Their prior record for monthly inflows was Rs 48,139 crore in May. This unprecedented monthly buying partially countered record monthly sales by foreign portfolio investors (FPIs) of Rs 1.1 trillion last month.

The Reserve Bank of India on Monday issued an operational framework for reclassification of investment made by a foreign portfolio investor to foreign direct investment (FDI) if the entity breaches the prescribed limit. Markets regulator Sebi too has issued a circular on procedure for reclassification of FPI investment to FDI.

From serving traditional meals in car parks to packaging coffee decoction, the city’s wedding caterers are finding innovative ways to power through the COVID-19 crisis

Tamil Nadu bodybuilders move to Chennai to pursue their dreams of a ripped physique, medals, fame, and a Government job

The firm is also looking at multiple strategies to deepen its activity in Southeast Asia.

The post China’s Cathay Capital eyes opportunities in digitalisation, life sciences appeared first on DealStreetAsia.

As the Jiomart platform takes off and increases in scale, there could be interesting possibilities that may emerge.

The post Guest Post | Implications and second-order effects of Jio-Facebook deal appeared first on DealStreetAsia.

Like man buns on scooters and ukulele busking, Covid-19 has now spread to the north from London – inspiring a coronavirus soapcom from our self-isolating comedy-writer

Up here in the north-west, we’re used to living in the slipstream of London’s sleek urban shenanigans. Whatever the cultural breakthrough – man buns on scooters, cashless ukulele busking, emotional support bees – it takes a while to reach the Lancaster and Morecambe Non-Metropolitan Area. If it ever does.

A Street Stranger Watch leads to a death and the appearance at midnight of the street’s original Victorian inhabitants

US President-elect Donald Trump has said he would take $1 as his salary a year and not the $400,000 that comes with the US president's job and will refrain from going on any vacation.

Company: P & I Management Consultants
Experience: 4 to 6
location: India
Ref: 24341091
Summary: Job Description: JD for Senior Production Engineer-Fabrication for reputed company manufacturing EOT/Overhead/Mobile Cranes is as below:- 1. Candidate should have experience of 4 -6 years in:- i. Fabrication Industry ii. MIG Welding....

Company: P & I Management Consultants
Experience: 6 to 9
location: India, Ludhiana
Ref: 24657059
Summary: Job Description: Job Description Graduate with over 6 years experience in Sales and Counseling for Immigration to Canada and arranging Study Visa for Canada. Must be expert in Agent-Networking for Immigration Services, ....

Earlier, we reported about Ram Gopal Varma's prediction on the deadly Coronavirus outbreak and social distancing. Well, the ace filmmaker has yet again stolen the limelight with a recent Corona song.  RGV has released a song on the deadly

Lewis Library - ML410.M595 C53 2018

Hayden Library - TA418.9.N35 F87 2018

A total of 7,740 dedicated Covid-19 facilities in 483 districts have been identified, the Union health ministry said on Sunday, asserting that adequa

Reluctant schools and teachers have been forced to adapt to e-learning

A total of 7,740 dedicated COVID-19 facilities in 483 districts have been identified, the Union health ministry said on Sunday, asserting that adequate health infrastructure is in place to fight the pandemic.

Princeton University has a substantial impact on the New Jersey economy, generating an annual total of $1.58 billion in economic output as an employer, research and innovation leader, sponsor of construction projects, purchaser of goods and services, and financial and civic contributor to local communities. That total supports an estimated 13,450 jobs with $970.7 million in earnings.

A Princeton University-led study has found that trees worldwide develop thicker bark when they live in fire-prone areas. The findings suggest that bark thickness could help predict which forests and savannas will survive a warmer climate in which wildfires are expected to increase in frequency.

BOSTON - The U.S. Environmental Protection Agency (EPA) is proposing targeted modifications to the 2016 Small Municipal Separate Storm Sewer System (MS4) general permit for Massachusetts communities.

BOSTON - The U.S. Environmental Protection Agency (EPA) is proposing targeted modifications to the 2017 Small Municipal Separate Storm Sewer System (MS4) general permit for New Hampshire communities.

PHILADELPHIA (April 29, 2020) — The U.S. Environmental Protection Agency announced today that Lea Wang, a sophomore at Council Rock High School in Holland, Pennsylvania, is the 2019 winner of a President’s Environmental Youth Award (PEYA) for EPA’s Mid-Atlantic Region.

PHILADELPHIA (April 29, 2020) – The U.S. Environmental Protection Agency announced today that science teacher Ann Strozyk from the Howard County Public School District in Maryland is a 2019 winner of a Presidential Innovation Award for Environmental Educators (PIAEE).

Environmental News  FOR IMMEDIATE RELEASE

Filed under: Skiing, North America, United States

• 16 Easy Ski Vacation Ideas from U.S. Cities
• 50 Ways to Ski in 50 States

Where to Ski In Every State and 16 Ski Vacations Near Big U.S. Cities originally appeared on Gadling on Thu, 05 Dec 2013 11:29:00 EST. Please see our terms for use of feeds.

Filed under: Internet Tools, News

Continue reading Facebook, Instagram Release Top Checked-in Locations of 2013

Facebook, Instagram Release Top Checked-in Locations of 2013 originally appeared on Gadling on Fri, 13 Dec 2013 14:23:00 EST. Please see our terms for use of feeds.

Filed under: North America, United States

Continue reading Sorry Indianapolis, You're No Longer the Sole Location Offering TSA PreCheck Enrollment Background Checks

Sorry Indianapolis, You're No Longer the Sole Location Offering TSA PreCheck Enrollment Background Checks originally appeared on Gadling on Mon, 16 Dec 2013 15:07:00 EST. Please see our terms for use of feeds.

While the phased-in reopening of schools in South Africa in the midst of Covid-19 remains provisional and sensitive, the Western Cape Education Department has advised principals and school management teams to wait a few more days before returning.

The PGA Tour's Memorial Tournament will use radio frequency identification chips in spectator badges to ensure fans maintain social distance.

Antibodies are widely used as cancer therapeutics, but their current use is limited by the low number of antigens restricted to cancer cells. A receptor tyrosine kinase, receptor tyrosine kinase-like orphan receptor 2 (ROR2), is normally expressed only during embryogenesis and is tightly down-regulated in postnatal healthy tissues. However, it is up-regulated in a diverse set of hematologic and solid malignancies, thus ROR2 represents a candidate antigen for antibody-based cancer therapy. Here we describe the affinity maturation and humanization of a rabbit mAb that binds human and mouse ROR2 but not human ROR1 or other human cell-surface antigens. Co-crystallization of the parental rabbit mAb in complex with the human ROR2 kringle domain (hROR2-Kr) guided affinity maturation by heavy-chain complementarity-determining region 3 (HCDR3)-focused mutagenesis and selection. The affinity-matured rabbit mAb was then humanized by complementarity-determining region (CDR) grafting and framework fine tuning and again co-crystallized with hROR2-Kr. We show that the affinity-matured and humanized mAb retains strong affinity and specificity to ROR2 and, following conversion to a T cell–engaging bispecific antibody, has potent cytotoxicity toward ROR2-expressing cells. We anticipate that this humanized affinity-matured mAb will find application for antibody-based cancer therapy of ROR2-expressing neoplasms.

β-Glucocerebrosidase (GBA) hydrolyzes glucosylceramide (GlcCer) to generate ceramide. Previously, we demonstrated that lysosomal GBA1 and nonlysosomal GBA2 possess not only GlcCer hydrolase activity, but also transglucosylation activity to transfer the glucose residue from GlcCer to cholesterol to form β-cholesterylglucoside (β-GlcChol) in vitro. β-GlcChol is a member of sterylglycosides present in diverse species. How GBA1 and GBA2 mediate β-GlcChol metabolism in the brain is unknown. Here, we purified and characterized sterylglycosides from rodent and fish brains. Although glucose is thought to be the sole carbohydrate component of sterylglycosides in vertebrates, structural analysis of rat brain sterylglycosides revealed the presence of galactosylated cholesterol (β-GalChol), in addition to β-GlcChol. Analyses of brain tissues from GBA2-deficient mice and GBA1- and/or GBA2-deficient Japanese rice fish (Oryzias latipes) revealed that GBA1 and GBA2 are responsible for β-GlcChol degradation and formation, respectively, and that both GBA1 and GBA2 are responsible for β-GalChol formation. Liquid chromatography–tandem MS revealed that β-GlcChol and β-GalChol are present throughout development from embryo to adult in the mouse brain. We found that β-GalChol expression depends on galactosylceramide (GalCer), and developmental onset of β-GalChol biosynthesis appeared to be during myelination. We also found that β-GlcChol and β-GalChol are secreted from neurons and glial cells in association with exosomes. In vitro enzyme assays confirmed that GBA1 and GBA2 have transgalactosylation activity to transfer the galactose residue from GalCer to cholesterol to form β-GalChol. This is the first report of the existence of β-GalChol in vertebrates and how β-GlcChol and β-GalChol are formed in the brain.

Lens proteins become increasingly cross-linked through nondisulfide linkages during aging and cataract formation. One mechanism that has been implicated in this cross-linking is glycation through formation of advanced glycation end products (AGEs). Here, we found an age-associated increase in stiffness in human lenses that was directly correlated with levels of protein–cross-linking AGEs. α-Crystallin in the lens binds to other proteins and prevents their denaturation and aggregation through its chaperone-like activity. Using a FRET-based assay, we examined the stability of the αA-crystallin–γD-crystallin complex for up to 12 days and observed that this complex is stable in PBS and upon incubation with human lens–epithelial cell lysate or lens homogenate. Addition of 2 mm ATP to the lysate or homogenate did not decrease the stability of the complex. We also generated complexes of human αA-crystallin or αB-crystallin with alcohol dehydrogenase or citrate synthase by applying thermal stress. Upon glycation under physiological conditions, the chaperone–client complexes underwent greater extents of cross-linking than did uncomplexed protein mixtures. LC-MS/MS analyses revealed that the levels of cross-linking AGEs were significantly higher in the glycated chaperone–client complexes than in glycated but uncomplexed protein mixtures. Mouse lenses subjected to thermal stress followed by glycation lost resilience more extensively than lenses subjected to thermal stress or glycation alone, and this loss was accompanied by higher protein cross-linking and higher cross-linking AGE levels. These results uncover a protein cross-linking mechanism in the lens and suggest that AGE-mediated cross-linking of α-crystallin–client complexes could contribute to lens aging and presbyopia.

Extracellular matrix-evoked angiostasis and autophagy within the tumor microenvironment represent two critical, but unconnected, functions of the small leucine-rich proteoglycan, decorin. Acting as a partial agonist of vascular endothelial growth factor 2 (VEGFR2), soluble decorin signals via the energy sensing protein, AMP-activated protein kinase (AMPK), in the autophagic degradation of intracellular vascular endothelial growth factor A (VEGFA). Here, we discovered that soluble decorin evokes intracellular catabolism of endothelial VEGFA that is mechanistically independent of mTOR, but requires an autophagic regulator, paternally expressed gene 3 (PEG3). We found that administration of autophagic inhibitors such as chloroquine or bafilomycin A1, or depletion of autophagy-related 5 (ATG5), results in accumulation of intracellular VEGFA, indicating that VEGFA is a basal autophagic substrate. Mechanistically, decorin increased the VEGFA clearance rate by augmenting autophagic flux, a process that required RAB24 member RAS oncogene family (RAB24), a small GTPase that facilitates the disposal of autophagic compartments. We validated these findings by demonstrating the physiological relevance of this process in vivo. Mice starved for 48 h exhibited a sharp decrease in overall cardiac and aortic VEGFA that could be blocked by systemic chloroquine treatment. Thus, our findings reveal a unified mechanism for the metabolic control of endothelial VEGFA for autophagic clearance in response to decorin and canonical pro-autophagic stimuli. We posit that the VEGFR2/AMPK/PEG3 axis integrates the anti-angiogenic and pro-autophagic bioactivities of decorin as the molecular basis for tumorigenic suppression. These results support future therapeutic use of decorin as a next-generation protein therapy to combat cancer.

Fucosylation of the innermost GlcNAc of N-glycans by fucosyltransferase 8 (FUT8) is an important step in the maturation of complex and hybrid N-glycans. This simple modification can dramatically affect the activities and half-lives of glycoproteins, effects that are relevant to understanding the invasiveness of some cancers, development of mAb therapeutics, and the etiology of a congenital glycosylation disorder. The acceptor substrate preferences of FUT8 are well-characterized and provide a framework for understanding N-glycan maturation in the Golgi; however, the structural basis of these substrate preferences and the mechanism through which catalysis is achieved remain unknown. Here we describe several structures of mouse and human FUT8 in the apo state and in complex with GDP, a mimic of the donor substrate, and with a glycopeptide acceptor substrate at 1.80–2.50 Å resolution. These structures provide insights into a unique conformational change associated with donor substrate binding, common strategies employed by fucosyltransferases to coordinate GDP, features that define acceptor substrate preferences, and a likely mechanism for enzyme catalysis. Together with molecular dynamics simulations, the structures also revealed how FUT8 dimerization plays an important role in defining the acceptor substrate-binding site. Collectively, this information significantly builds on our understanding of the core fucosylation process.

As daunting as this COVID-19 pandemic has proven to be, and as devastating as its impact is on people both here at home and around the world, we must stand together, united. We must all act with purpose, resolve, love and compassion because when we...

At least 10 stores inside the Portmore Mall have fallen casualty to the economic chokehold brought on by the COVID-19 pandemic, with several others struggling to stay afloat as St Catherine businesses grapple with revenue losses. And with a 14-day...

MPI’s National Center on Immigrant Integration Policy convened a major public policy research symposium focused on young children of immigrants in the U.S.

A webinar showcasing MPI's profiles of unauthorized immigrants in the 94 U.S. counties with the largest populations potentially eligible for DACA or DAPA, and the implications of the data for implementation of the DACA and DAPA programs.

This report provides an overview of several commonly used translation and interpretation technologies. It aims to assist language access practitioners in understanding and identifying which systems would best meet their agency’s language access needs.

Many of Sweden's immigrants are refugees who lack the skills and education to gain employment soon after they arrive. Over time, however, newcomers to Sweden have improved their employment rates, displayed income growth similar to natives, and moved from low- to middle-skilled positions. This report assesses how new immigrants—refugees, labor migrants, and others—fare in Sweden's labor market.

Part of a series exploring issues likely to be addressed by the new National Integration Plan, this webinar, with perspectives from MPI, the National Partnership for New Americans, and the National Skills Coalition, looks at the role of adult education and English language and skills training in the immigrant integration process.

This webinar, with perspectives from MPI, the National Partnership for New Americans, and the National Skills Coalition, looks at the role of adult education and English language and skills training in the immigrant integration process.

Testimony of Marc R. Rosenblum, Deputy Director, U.S. Immigration Program, before the House Committee on Homeland Security Subcommittee on Oversight and Management Efficiency.