Got me boo'd up, boo'd up!

She's coming for all of us.

Poor, dim-witted humanity. We used to think we were the center of everything. That wasn’t that long ago, and even though we’ve made tremendous advancements in our understanding of our situation here in space, we still have huge blind spots. For one, we’re only now waking up to the reality of interstellar objects passing through …

Continue reading "A Cool Idea to Catch Up With an Interstellar Visitor"

The post A Cool Idea to Catch Up With an Interstellar Visitor appeared first on Universe Today.

Removed From Troubles (OPEN for application) Fonts: Pixel / Bitmap (Graphics / Layouts / Effects); Domains (.com) (Web Miscellany)

The post Reel Review: What’s New with the Shimano Stella SW 2020? appeared first on Ocean Blue Fishing Adventures.

[Image: An example of gravitational lens effects, via Wikipedia.] Over at WIRED, Daniel Oberhaus, author of the recent book Extraterrestrial Languages, takes a look at some proposals from NASA’s Innovative Advanced Concept (NIAC) program. “Among this year’s NIAC grants,” Oberhaus writes, “are proposals to turn a lunar crater into a giant radio dish, to develop … Continue reading "Gravitational Lensing, Interstellar Cinematography, and the Future of Magical Warfare in Space"

An interview with Viv Clelland, winner of the ’The untold story’ award at the Scottish Social Services Awards 2019. She is a support worker for looked after young people and families in East Ayrshire. 

Viv lived in a Kilmarnock children’s home from the age of 11 years until she was 17. She began her career doing a bit of everything: cleaning, working in bars, as well as volunteering for a horse riding school for disabled young people.

In 2000, she became a volunteer parent coach with Kilmarnock Harriers, which led to her being approached to volunteer with East Ayrshire Council. By 2006, she was employed as Sports Motivator – working in a variety of community coaching & children’s house sessions for the council.

As Viv developed, the job developed and her role became more focused on working with looked after and accommodated young people, based within the play and early intervention team. Her skills have also supported work in HMP Kilmarnock and with vulnerable families. 

Transcript of episode

Music Credit: Make your dream a reality by Scott Holmes.

This week: we go through the week's Potter news, including the new Wizarding World app as well as the upcoming release of the graphic edition of Harry Potter and the Goblet of Fire!

This week's What About That: Arabella Figg is here to change all our SEO results. Who was she? What's her history? Was she married? Was she gay?

Also: squibs in the wizarding world: what does it mean to be a squib? What's the deal with the book and the quill? Why was it mean to Neville? WHY WOULD ANYONE EVER BE MEAN TO NEVILLE?

Also this week from Mischief Media: Listen to A Story Most Queer, now available wherever you listen!

Don't forget that we have a Patreon where you can get extra (sorting-related!) content!

By the way: never listen to what number we call the show inside the recording. We're gonna stop doing that.

Also, in the drums: John explains Brexit! 

Produced by Adam Molina // Assoc. Produced by Kylie Madden

ACCC had threatened legal action

But Australia-New Zealand links could ramp up again in coming months

Agents' commission 'will be fully protected'

Shanghai Disneyland to reopen on Monday - but guests must wear masks

Asia and Middle East cruises axed

It was not the aching feet that were stopping me in my tracks . To be fair they were burning . This walking plays havoc with the arches of your feet . It was not my brain working overtime thinking of reasons not to go out . Although it would have been easy

Astronomer Avi Loeb believes that the interstellar object dubbed 'Oumuamua could actually be a probe sent by alien beings. Given the evidence that has so far been gathered, he says, it is a possible conclusion to draw.

New rules from federal Education Secretary Betsy DeVos would severely limit students' ability to clear debt by burying them in bureaucratic red tape, according to a new federal lawsuit.

Rugby Football Union chief executive Bill Sweeney has described the prospect of the sport being postponed into 2021 as "catastrophic".

The comet, 2I/Borisov, comes from another planetary system, but bears a remarkable resemblance to local space rocks.

Bernie Sanders supporters lash out after New York cancels its presidential primary, saying Democrats are trying to deny the former candidate a voice at the convention.

The Times is launching enhanced digital coverage of California politics, an effort that will be overseen by Christina Bellantoni, who currently is editor in chief of Roll Call, the Capitol Hill newspaper. 

Kings' coach Todd McLellan was thrilled with progress of streaking Kings before season was stopped, but wishes he could have seen how group endured.

A decade in the making, Tyra Banks' modeling utopia, ModelLand, 'will emulate a fantasy version of the modeling world.' The park opens in Santa Monica in May.

Vegan Fashion Week will livestream its runway behind closed doors. "The public event might be canceled, but the message cannot be," says founder.

Times fashion critic Adam Tschorn offers his thoughts on Episodes 7 and 8 of Amazon's fashion competition 'Making the Cut.'

Peter Sellars — opera director, spiritual thinker, optimist — reflects on changes triggered by coronavirus. Amid tragedy, what new life might come forth?

After tossing his Scottsdale, Ariz., home on the market last year, retired pitcher Mark Mulder has sold the amenity-loaded home for $1.81 million.

In Pasadena, the E. Lawrence Brown House, a stellar example of the Spanish Colonial Revival style, is for sale at $4.25 million.

Britain's biggest holiday company has axed nearly 900,000 trips so far

The Ryder Cup remains scheduled to take place in September despite other major golf tournaments being postponed.

Rafael Nadal revealed this week he was doubtful there will be further tennis in 2020.

Danville senior Ella Collier is Hendricks County's all-time leading scorer. And it didn't happen by accident.

Also look for a hot chicken food truck and another location in Bloomington.

Fans of the Disney animated movie celebrated its addition to the National Film Registry.

Some House Democrats have decided that the COVID-19 pandemic is an appropriate time to implement a key plank of the campaign of former Democratic presidential candidate Sen. Bernie Sanders of Vermont. Reps. Ilhan Omar of Minnesota, Rashida Tlaib of Michigan, Alexandria Ocasio-Cortez of New York and Ayanna Pressley of Massachusetts — who make up the…

The post ‘The Squad’ Pushes for Huge Student Loan Cancellation in Next Relief Bill appeared first on The Western Journal.

The transfer of a phosphate from ATP to a protein substrate, a modification known as protein phosphorylation, is catalyzed by protein kinases. Protein kinases play a crucial role in virtually every cellular activity. Recent studies of atypical protein kinases have highlighted the structural similarity of the kinase superfamily despite notable differences in primary amino acid sequence. Here, using a bioinformatics screen, we searched for putative protein kinases in the intracellular bacterial pathogen Legionella pneumophila and identified the type 4 secretion system effector Lpg2603 as a remote member of the protein kinase superfamily. Employing an array of biochemical and structural biology approaches, including in vitro kinase assays and isothermal titration calorimetry, we show that Lpg2603 is an active protein kinase with several atypical structural features. Importantly, we found that the eukaryote-specific host signaling molecule inositol hexakisphosphate (IP6) is required for Lpg2603 kinase activity. Crystal structures of Lpg2603 in the apo-form and when bound to IP6 revealed an active-site rearrangement that allows for ATP binding and catalysis. Our results on the structure and activity of Lpg2603 reveal a unique mode of regulation of a protein kinase, provide the first example of a bacterial kinase that requires IP6 for its activation, and may aid future work on the function of this effector during Legionella pathogenesis.

Systemic infection and proliferation of intracellular pathogens require the biogenesis of a growth-stimulating compartment. The gastrointestinal pathogen Salmonella enterica commonly forms highly dynamic and extensive tubular membrane compartments built from Salmonella-modified membranes (SMMs) in diverse host cells. Although the general mechanism involved in the formation of replication-permissive compartments of S. enterica is well researched, much less is known regarding specific adaptations to different host cell types. Using an affinity-based proteome approach, we explored the composition of SMMs in murine macrophages. The systematic characterization provides a broader landscape of host players to the maturation of Salmonella-containing compartments and reveals core host elements targeted by Salmonella in macrophages as well as epithelial cells. However, we also identified subtle host specific adaptations. Some of these observations, such as the differential involvement of the COPII system, Rab GTPases 2A, 8B, 11 and ER transport proteins Sec61 and Sec22B may explain cell line-dependent variations in the pathophysiology of Salmonella infections. In summary, our system-wide approach demonstrates a hitherto underappreciated impact of the host cell type in the formation of intracellular compartments by Salmonella.

The transfer of a phosphate from ATP to a protein substrate, a modification known as protein phosphorylation, is catalyzed by protein kinases. Protein kinases play a crucial role in virtually every cellular activity. Recent studies of atypical protein kinases have highlighted the structural similarity of the kinase superfamily despite notable differences in primary amino acid sequence. Here, using a bioinformatics screen, we searched for putative protein kinases in the intracellular bacterial pathogen Legionella pneumophila and identified the type 4 secretion system effector Lpg2603 as a remote member of the protein kinase superfamily. Employing an array of biochemical and structural biology approaches, including in vitro kinase assays and isothermal titration calorimetry, we show that Lpg2603 is an active protein kinase with several atypical structural features. Importantly, we found that the eukaryote-specific host signaling molecule inositol hexakisphosphate (IP6) is required for Lpg2603 kinase activity. Crystal structures of Lpg2603 in the apo-form and when bound to IP6 revealed an active-site rearrangement that allows for ATP binding and catalysis. Our results on the structure and activity of Lpg2603 reveal a unique mode of regulation of a protein kinase, provide the first example of a bacterial kinase that requires IP6 for its activation, and may aid future work on the function of this effector during Legionella pathogenesis.

(NASA/Goddard Space Flight Center) A bustling stellar nursery in the picturesque Orion Nebula will be a subject of study for NASA's James Webb Space Telescope, scheduled to launch in 2021. A team led by Mark McCaughrean, the Webb Interdisciplinary Scientist for Star Formation, will survey an inner region of the nebula called the Trapezium Cluster. This cluster is home to a thousand or so young stars, all crammed into a space only 4 light-years across -- about the distance from our Sun to Alpha Centauri.

(US Department of Agriculture - Agricultural Research Service) A new freeze-resistant Trichinella species has been discovered in wolverines by Agricultural Research Service scientists and their colleagues. Trichinella are parasites that cause the disease trichinosis (formally referred to as trichinellosis), which people can get by eating raw or undercooked meat from infected animals.

Georg H H Borner
Apr 28, 2020; 0:R120.001971v1-mcp.R120.001971
Review

Systemic infection and proliferation of intracellular pathogens require the biogenesis of a growth-stimulating compartment. The gastrointestinal pathogen Salmonella enterica commonly forms highly dynamic and extensive tubular membrane compartments built from Salmonella-modified membranes (SMMs) in diverse host cells. Although the general mechanism involved in the formation of replication-permissive compartments of S. enterica is well researched, much less is known regarding specific adaptations to different host cell types. Using an affinity-based proteome approach, we explored the composition of SMMs in murine macrophages. The systematic characterization provides a broader landscape of host players to the maturation of Salmonella-containing compartments and reveals core host elements targeted by Salmonella in macrophages as well as epithelial cells. However, we also identified subtle host specific adaptations. Some of these observations, such as the differential involvement of the COPII system, Rab GTPases 2A, 8B, 11 and ER transport proteins Sec61 and Sec22B may explain cell line-dependent variations in the pathophysiology of Salmonella infections. In summary, our system-wide approach demonstrates a hitherto underappreciated impact of the host cell type in the formation of intracellular compartments by Salmonella.

Niemann-Pick disease, type C1 (NPC1) is a lipid storage disorder in which cholesterol and glycosphingolipids accumulate in late endosomal/lysosomal compartments because of mutations in the NPC1 gene. A hallmark of NPC1 is progressive neurodegeneration of the cerebellum as well as visceral organ damage; however, the mechanisms driving this disease pathology are not fully understood. Phosphoinositides are phospholipids that play distinct roles in signal transduction and vesicle trafficking. Here, we utilized consensus spectra analysis of MS imaging datasets and orthogonal LC–MS analyses to evaluate the spatial distribution of phosphoinositides and quantify them in cerebellar tissue from Npc1-null mice. Our results suggest significant depletion of multiple phosphoinositide species, including phosphatidylinositol (PI), phosphatidylinositol monophosphate (PIP), and bisphosphate (PIP2), in the cerebellum of the Npc1-null mice in both whole-tissue lysates and myelin-enriched fractions. Additionally, we observed altered levels of the regulatory enzyme phosphatidylinositol 4-kinase type 2 α (PI4K2A) in Npc1-null mice. In contrast, the levels of related kinases, phosphatases, and transfer proteins were unaltered in the Npc1-null mouse model as observed by Western blot analysis. Our discovery of phosphoinositide lipid biomarkers for NPC1 opens new perspectives on the pathophysiology underlying this fatal neurodegenerative disease.

Protein subcellular localization is an essential and highly regulated determinant of protein function. Major advances in mass spectrometry and imaging have allowed the development of powerful spatial proteomics approaches for determining protein localization at the whole cell scale. Here, a brief overview of current methods is presented, followed by a detailed discussion of organellar mapping through proteomic profiling. This relatively simple yet flexible approach is rapidly gaining popularity, due to its ability to capture the localizations of thousands of proteins in a single experiment. It can be used to generate high-resolution cell maps, and as a tool for monitoring protein localization dynamics. This review highlights the strengths and limitations of the approach, and provides guidance to designing and interpreting profiling experiments. 

The transfer of a phosphate from ATP to a protein substrate, a modification known as protein phosphorylation, is catalyzed by protein kinases. Protein kinases play a crucial role in virtually every cellular activity. Recent studies of atypical protein kinases have highlighted the structural similarity of the kinase superfamily despite notable differences in primary amino acid sequence. Here, using a bioinformatics screen, we searched for putative protein kinases in the intracellular bacterial pathogen Legionella pneumophila and identified the type 4 secretion system effector Lpg2603 as a remote member of the protein kinase superfamily. Employing an array of biochemical and structural biology approaches, including in vitro kinase assays and isothermal titration calorimetry, we show that Lpg2603 is an active protein kinase with several atypical structural features. Importantly, we found that the eukaryote-specific host signaling molecule inositol hexakisphosphate (IP6) is required for Lpg2603 kinase activity. Crystal structures of Lpg2603 in the apo-form and when bound to IP6 revealed an active-site rearrangement that allows for ATP binding and catalysis. Our results on the structure and activity of Lpg2603 reveal a unique mode of regulation of a protein kinase, provide the first example of a bacterial kinase that requires IP6 for its activation, and may aid future work on the function of this effector during Legionella pathogenesis.

Nicky Riemer from Union Dining is one of 20 of Victoria's top chefs who will unite to raise funds for vulnerable children at Gastronomique on November 25. The gala food and wine charity evening will support Anglicare Victoria. Now in its 12th year, Gastronomique includes signature dishes, fine wine and entertainment. www.gastronomique.org.au