Company: S R IT Services
Experience: 0 to 5
location: Indore, Nashik
Ref: 24731858
Summary: FMCG Production and Quality Requirements and also Food Beverages.

Company: P & I Management Consultants
Experience: 6 to 9
location: India, Ludhiana
Ref: 24657059
Summary: Job Description: Job Description Graduate with over 6 years experience in Sales and Counseling for Immigration to Canada and arranging Study Visa for Canada. Must be expert in Agent-Networking for Immigration Services, ....

Company: SP Staffing Services Private Limited
Qualification: Other
Experience: 1 to 5
Salary: 3.50 to 6.10
location: Chennai
Ref: 24374534
Summary: Immediate Hiring For Python Developer

A trailblazing satirist who battled racial injustice in America

Healthcare for the unemployed set to become key election issue

Athlete's foot (AF), also known as tinea pedis is a fungal infection of the foot, mostly found between the toes. It is named so as the infection is common in athletes and swimmers. The AF can spread to hands or other

Here are the key developments related to Coronavirus pandemic on May 9.

As part of the Mission Samudra Setu, INS Jalashwa reached Maldives on May 7 to evacuate Indian nationals.

Lewis Library - ML410.M382 A3 2018

Lewis Library - ML423.B52 A4 2018

An Air India flight landed here from London at 1.30 am on Sunday carrying 239 Indians from the UK who were stranded there due to suspension of commer

The time has come for India to adopt a “whole-of-government approach” in decisively confronting the “strategic uncertainties” looming on its horizon a

Coal Secretary Anil Jain said FY20 coal production was lower than the target of 660 million tonnes because of flooding of a key coal mine.

HDFC Bank says “minuscule” number of employees told to leave as part of annual appraisal process

Convincing migrating students to stay with domestic options post-Covid will require sweeping reforms in the education system

Railways will start online reservation for special trains with effect from 4 pm on May 11

Reluctant schools and teachers have been forced to adapt to e-learning

But, logistic issues could torpedo the plan

Fiscal constraints should be put aside to support the country through Covid-19

Before this, David and his wife Candice were seen grooving to recent Telugu hit track Butta Bomma from Allu Arjun starrer Ala Vaikuntapuramlo.

News reports suggest that a total of 150 soldiers were involved in the face-off that allegedly took place a few days back.

This deployment is in line with India's role as the first responder in the region.

1. South Korea takes first cautious steps into a post-Covid world  The Guardian
2. S. Korea warned of COVID-10 second wave  NEWS.com.au
3. South Korea reverses on reopenings amid nightclub outbreak  The Age
4. S.Korea leader says no panic as cases rise  SBS News
5. Coronavirus: How South Korea 'crushed' the curve  BBC News
6. View Full coverage on Google News

1. NSW warns ‘complacency biggest threat’ as most states move to ease Covid-19 restrictions  The Guardian
2. NSW coronavirus restrictions to lift from Friday, but will not follow all National Cabinet measures  ABC News
3. Coronavirus Australia: NSW Premier issues firm warning to NSW residents  Jimmys Post
4. NSW to ease lockdown restrictions from Friday  Sydney Morning Herald
5. NSW Premier Gladys Berejiklian says relaxation of restrictions doesn't mean coronavirus is less deadly  ABC News
6. View Full coverage on Google News

1. Ten arrested and police officer injured at protest against Victoria’s Covid-19 lockdown laws  The Guardian
2. Hundreds of anti-vaxxers clash with police during protest in Melbourne's CBD  Daily Mail
3. Coronavirus: Anti-lockdown protesters clash with police in Melbourne  NEWS.com.au
4. Ten arrested, police officer injured in anti-lockdown protest  The Age
5. Coronavirus Australia updates: anti-lockdown protest in Melbourne as NSW and WA set to ease Covid-19 restrictions – as it happened  The Guardian
6. View Full coverage on Google News

Dr. Dillon Diaz is accepting new patients, he specializes in deep cavity research. See more at NoirMale.

The post NoirMale: Dillon Diaz appeared first on QueerClick.

WASHINGTON (April 28, 2020) —  Today, the U.S. Environmental Protection Agency (EPA) and State of Indiana announced that recent air monitoring data show the city of Indianapolis, and the areas of Muncie, Lake and Porter counties are meeting federal air quality standards.

Games involving Notre Dame, Purdue and Indiana could have a lot at stake this season.

Now you can read all of IndyStar's sports content in one place with our free INSports app.

From Notre Dame to the Monon Bell. We rank Indiana's top 50 college football games in 2018.

Indianapolis Metropolitan Police Department are on the scene in the area of East 32nd Street and Baltimore Avenue.

Indianapolis Metropolitan Police Department are on the scene in the area of East 32nd Street and Baltimore Avenue.

Long-time Slashdot reader UnknowingFool writes: As NVidia and AMD battle it out with their GPUs in consumer gaming, behind the scenes both manufacturers were competing against each for manufacturing capacity with TSMC's lines. It appears that early on in the 7nm line, NVidia lost out as it is rumored their strategy was to use Samsung more only to go back to TSMC when that failed By then TSMC had sold out their capacity to AMD. For their next generation GPUs it appears both NVidia is securing both 5nm early while grabbing as much 7nm as possible.

Read more of this story at Slashdot.

1. Earthquake of magnitude 3.5 hits Delhi-NCR  Times of India
2. Magnitude 3.4 Earthquake Strikes Delhi, Epicentre Near UP Border  News18
3. 3.5 Magnitude Earthquake Hits Delhi-NCR, no Casualty Reported  India.com
4. Breaking news live : Eathquake tremors felt in Delhi, adjoining areas  Times of India
5. Earthquake in Delhi: Tremors felt in national capital region  The Financial Express
6. View Full coverage on Google News

Apple India Website Puts Siri in Silent Mode

Dr John Wright is intrigued by some singers who became ill long before the UK's first known Covid-19 case.

Filed under: North America, United States

Continue reading Sorry Indianapolis, You're No Longer the Sole Location Offering TSA PreCheck Enrollment Background Checks

Sorry Indianapolis, You're No Longer the Sole Location Offering TSA PreCheck Enrollment Background Checks originally appeared on Gadling on Mon, 16 Dec 2013 15:07:00 EST. Please see our terms for use of feeds.

Canadians working aboard two cruise ships who weren't allowed to come to shore because of concerns about COVID-19 are finally able to return home.

27 January 2015

Members Event

Event participants

Dr Mukulika Banerjee, Director, South Asia Centre, London School of Economics
Nandan Nilekani, Co-founder and Chairman, EkStep; Chairman, Unique Identification Authority of India (UIAI) (2009-14)
Dr Gareth Price, Senior Research Fellow, Asia Programme, Chatham House
Mihir Swarup Sharma, India Columnist, Bloomberg View; Senior Fellow, Observer Research Foundation, New Delhi
Chair: James Crabtree, Contributing Editor, Financial Times; Senior Visiting Fellow, Lee Kuan Yew School of Public Policy

6 January 2017 , Volume 93, Number 1

South Dakota Governor Kristi Noem sent letters to two Sioux tribes demanding they remove COVID-19 checkpoints because they interfere with traffic.

Nepal protested India's inauguration of a new road to China that passes through territory claimed by Kathmandu on Saturday, with police arresting dozens demonstrating close to India's embassy. Indian defence minister Rajnath Singh on Friday inaugurated via video link the 80-kilometre (50 miles) long road from Ghatiabagarh in northern Uttarakhand state to the Lipu Lekh pass high in the Himalaya. The pass is claimed by Nepal based on an 1816 treaty that defines its western border with India.

In a tweet Saturday morning, Tesla's chief executive said it would file a lawsuit against county officials over not being able to run its factory.

Although a robust inflammatory response is needed to combat infection, this response must ultimately be terminated to prevent chronic inflammation. One mechanism that terminates inflammatory signaling is the production of alternative mRNA splice forms in the Toll-like receptor (TLR) signaling pathway. Whereas most genes in the TLR pathway encode positive mediators of inflammatory signaling, several, including that encoding the MyD88 signaling adaptor, also produce alternative spliced mRNA isoforms that encode dominant-negative inhibitors of the response. Production of these negatively acting alternatively spliced isoforms is induced by stimulation with the TLR4 agonist lipopolysaccharide (LPS); thus, this alternative pre-mRNA splicing represents a negative feedback loop that terminates TLR signaling and prevents chronic inflammation. In the current study, we investigated the mechanisms regulating the LPS-induced alternative pre-mRNA splicing of the MyD88 transcript in murine macrophages. We found that 1) the induction of the alternatively spliced MyD88 form is due to alternative pre-mRNA splicing and not caused by another RNA regulatory mechanism, 2) MyD88 splicing is regulated by both the MyD88- and TRIF-dependent arms of the TLR signaling pathway, 3) MyD88 splicing is regulated by the NF-κB transcription factor, and 4) NF-κB likely regulates MyD88 alternative pre-mRNA splicing per se rather than regulating splicing indirectly by altering MyD88 transcription. We conclude that alternative splicing of MyD88 may provide a sensitive mechanism that ensures robust termination of inflammation for tissue repair and restoration of normal tissue homeostasis once an infection is controlled.

Inter-α-inhibitor is a proteoglycan essential for mammalian reproduction and also plays a less well-characterized role in inflammation. It comprises two homologous “heavy chains” (HC1 and HC2) covalently attached to chondroitin sulfate on the bikunin core protein. Before ovulation, HCs are transferred onto the polysaccharide hyaluronan (HA) to form covalent HC·HA complexes, thereby stabilizing an extracellular matrix around the oocyte required for fertilization. Additionally, such complexes form during inflammatory processes and mediate leukocyte adhesion in the synovial fluids of arthritis patients and protect against sepsis. Here using X-ray crystallography, we show that human HC1 has a structure similar to integrin β-chains, with a von Willebrand factor A domain containing a functional metal ion-dependent adhesion site (MIDAS) and an associated hybrid domain. A comparison of the WT protein and a variant with an impaired MIDAS (but otherwise structurally identical) by small-angle X-ray scattering and analytical ultracentrifugation revealed that HC1 self-associates in a cation-dependent manner, providing a mechanism for HC·HA cross-linking and matrix stabilization. Surprisingly, unlike integrins, HC1 interacted with RGD-containing ligands, such as fibronectin, vitronectin, and the latency-associated peptides of transforming growth factor β, in a MIDAS/cation-independent manner. However, HC1 utilizes its MIDAS motif to bind to and inhibit the cleavage of complement C3, and small-angle X-ray scattering–based modeling indicates that this occurs through the inhibition of the alternative pathway C3 convertase. These findings provide detailed structural and functional insights into HC1 as a regulator of innate immunity and further elucidate the role of HC·HA complexes in inflammation and ovulation.

Lens proteins become increasingly cross-linked through nondisulfide linkages during aging and cataract formation. One mechanism that has been implicated in this cross-linking is glycation through formation of advanced glycation end products (AGEs). Here, we found an age-associated increase in stiffness in human lenses that was directly correlated with levels of protein–cross-linking AGEs. α-Crystallin in the lens binds to other proteins and prevents their denaturation and aggregation through its chaperone-like activity. Using a FRET-based assay, we examined the stability of the αA-crystallin–γD-crystallin complex for up to 12 days and observed that this complex is stable in PBS and upon incubation with human lens–epithelial cell lysate or lens homogenate. Addition of 2 mm ATP to the lysate or homogenate did not decrease the stability of the complex. We also generated complexes of human αA-crystallin or αB-crystallin with alcohol dehydrogenase or citrate synthase by applying thermal stress. Upon glycation under physiological conditions, the chaperone–client complexes underwent greater extents of cross-linking than did uncomplexed protein mixtures. LC-MS/MS analyses revealed that the levels of cross-linking AGEs were significantly higher in the glycated chaperone–client complexes than in glycated but uncomplexed protein mixtures. Mouse lenses subjected to thermal stress followed by glycation lost resilience more extensively than lenses subjected to thermal stress or glycation alone, and this loss was accompanied by higher protein cross-linking and higher cross-linking AGE levels. These results uncover a protein cross-linking mechanism in the lens and suggest that AGE-mediated cross-linking of α-crystallin–client complexes could contribute to lens aging and presbyopia.

The dextransucrase DSR-OK from the Gram-positive bacterium Oenococcus kitaharae DSM17330 produces a dextran of the highest molar mass reported to date (∼109 g/mol). In this study, we selected a recombinant form, DSR-OKΔ1, to identify molecular determinants involved in the sugar polymerization mechanism and that confer its ability to produce a very-high-molar-mass polymer. In domain V of DSR-OK, we identified seven putative sugar-binding pockets characteristic of glycoside hydrolase 70 (GH70) glucansucrases that are known to be involved in glucan binding. We investigated their role in polymer synthesis through several approaches, including monitoring of dextran synthesis, affinity assays, sugar binding pocket deletions, site-directed mutagenesis, and construction of chimeric enzymes. Substitution of only two stacking aromatic residues in two consecutive sugar-binding pockets (variant DSR-OKΔ1-Y1162A-F1228A) induced quasi-complete loss of very-high-molar-mass dextran synthesis, resulting in production of only 10–13 kg/mol polymers. Moreover, the double mutation completely switched the semiprocessive mode of DSR-OKΔ1 toward a distributive one, highlighting the strong influence of these pockets on enzyme processivity. Finally, the position of each pocket relative to the active site also appeared to be important for polymer elongation. We propose that sugar-binding pockets spatially closer to the catalytic domain play a major role in the control of processivity. A deep structural characterization, if possible with large-molar-mass sugar ligands, would allow confirming this hypothesis.

Contact between inflammatory cells and endothelial cells (ECs) is a crucial step in vascular inflammation. Recently, we demonstrated that the cell-surface level of endomucin (EMCN), a heavily O-glycosylated single-transmembrane sialomucin, interferes with the interactions between inflammatory cells and ECs. We have also shown that, in response to an inflammatory stimulus, EMCN is cleared from the cell surface by an unknown mechanism. In this study, using adenovirus-mediated overexpression of a tagged EMCN in human umbilical vein ECs, we found that treatment with tumor necrosis factor α (TNF-α) or the strong oxidant pervanadate leads to loss of cell-surface EMCN and increases the levels of the C-terminal fragment of EMCN 3- to 4-fold. Furthermore, treatment with the broad-spectrum matrix metalloproteinase inhibitor batimastat (BB94) or inhibition of ADAM metallopeptidase domain 10 (ADAM10) and ADAM17 with two small-molecule inhibitors, GW280264X and GI254023X, or with siRNA significantly reduced basal and TNFα-induced cell-surface EMCN cleavage. Release of the C-terminal fragment of EMCN by TNF-α treatment was blocked by chemical inhibition of ADAM10 alone or in combination with ADAM17. These results indicate that cell-surface EMCN undergoes constitutive cleavage and that TNF-α treatment dramatically increases this cleavage, which is mediated predominantly by ADAM10 and ADAM17. As endothelial cell-surface EMCN attenuates leukocyte–EC interactions during inflammation, we propose that EMCN is a potential therapeutic target to manage vascular inflammation.