A large-scale update of the file names used for articles available via the PMC FTP service for bulk download was undertaken in early January 2017. The new file naming convention is PMCID-based (e.g., PMC4855680.tar.gz) rather than being built from article citation data (i.e., journal abbreviation_pub date_volume_issue_page). This update was made following user reports that the previous naming convention was resulting in missing contents in cases where citation data was duplicated across multiple articles. The new convention will ensure that file names are unique and that the corpus available via the FTP service is complete.

Peer review documents, including review reports and editor decision letters, are increasingly being published along with the articles they review. This practice is intended to make the publishing process more transparent. To support these efforts, PMC’s Tagging Guidelines have been updated to include the tagging of peer review documents. NLM encourages PMC-participating publishers, journals, and data providers to review this guidance. Please contact us at pubmedcentral@ncbi.nlm.nih.gov if you have any questions.

Title: Middle Age More Stressful Now Than in 1990s: Study
Category: Health News
Created: 5/7/2020 12:00:00 AM
Last Editorial Review: 5/8/2020 12:00:00 AM

Purpose: Untreated and poorly controlled diabetes causes increased levels of blood glucose associated with poor periodontal disease outcomes. Dental hygienists can play a significant role in screening patients for diabetes mellitus, leading to referral and early diagnosis. The purpose of this study was to determine the knowledge, attitudes, practices, and barriers faced by clinical dental hygienists regarding diabetes risk assessment and screenings.Methods: A mixed method design was used with a convenience sample of dental hygienists in clinical practice (n=316). A 32 item, electronic survey was validated at item-level, and participants were recruited through multiple dental hygiene Facebook groups. Descriptive statistics were used to analyze the data. The survey also included two open-ended attitude questions that were interpreted using thematic analysis to pinpoint common patterns within the data.Results: Dental hygienists had high knowledge scores regarding diabetes and oral health, although many were unaware of their states' specific statutes and regulations for screening practices. Nearly all (95.9%), were likely to educate and refer patients (82%), although fewer than half (40.9%), were likely to perform chairside screening for diabetes. Emergent themes for barriers to screening were time, money, patient acceptance/willingness, lack of education, not having the proper tools, and states' rules and regulations.Conclusion: Despite high knowledge scores regarding diabetes and oral health, there is a gap in regards to dental hygienists' willingness to perform diabetes screenings in a clinical setting. Dental hygienists should be capable of integrating chairside diabetes screening practices into the process of care with proper training.

In a nod to the people who came before them — and those who still live among them — the Minnesota Public Health Association is acknowledging ancestral lands.

In recent years, there have been intensive debates on whether healthy adults acquire new word knowledge through fast mapping (FM) by a different mechanism from explicit encoding (EE). In this study, we focused on this issue and investigated to what extent retention interval, prior knowledge (PK), and lure type modulated memory after FM and EE. Healthy young participants were asked to learn novel word-picture associations through both FM and EE. Half of the pictures were from familiar categories (i.e., high PK) and the other half were from unfamiliar categories (i.e., low PK). After 10 min and 1 wk, the participants were tested by forced-choice (FC) tasks, with lures from different categories (Experiment 1) or from the same categories of the target pictures (Experiment 2). Pseudowords were used to denote names of the novel pictures and baseline performance was controlled for each task. The results showed that in both Experiments 1 and 2, memory performance remained stable after FM, while it declined after EE from 10 min to 1 wk. Moreover, the effect of PK appeared at 10 min after FM while at 1 wk after EE in Experiment 2. PK enhanced memory of word-picture associations when the lures were from the same categories (Experiment 2), rather than from different categories (Experiment 1). These results were largely confirmed in Experiment 3 when encoding condition was manipulated as a between-subjects factor, while lure type as a within-subjects factor. The findings suggest that different from EE, FM facilitates rapid acquisition and consolidation of word-picture knowledge, and highlight that PK plays an important role in this process by enhancing access to detailed information.

The Henry Buckley Collection of Planktonic Foraminifera at the Natural History Museum in London (NHMUK) consists of 1665 single-taxon slides housing 23 897 individuals from 203 sites in all the major ocean basins, as well as a vast research library of Scanning Electron Microscope (SEM) photomicrographs. Buckley picked the material from the NHMUK Ocean-Bottom Deposit Collection and also from fresh tow samples. However, his collection remains largely unused as he was discouraged by his managers in the Mineralogy Department from working on or publicizing the collection. Nevertheless, Buckley published pioneering papers on isotopic interpretation of oceanographic and climatic change and was one of the first workers to investigate foraminiferal wall structure using the SEM technique. Details of the collection and images of each slide are available via the NHMUK Data Portal (http://dx.doi.org/10.5519/0035055). The Buckley Collection and its associated Ocean-Bottom Deposit Collection have great potential for taxon-specific studies as well as geochemical work, and both collections are available on request.

Quinolones, such as the antimalarial atovaquone, are inhibitors of the malarial mitochondrial cytochrome bc₁ complex, a target critical to the survival of both liver- and blood-stage parasites, making these drugs useful as both prophylaxis and treatment. Recently, several derivatives of endochin have been optimized to produce novel quinolones that are active in vitro and in animal models. While these quinolones exhibit potent ex vivo activity against Plasmodium falciparum and Plasmodium vivax, their activity against the zoonotic agent Plasmodium knowlesi is unknown. We screened several of these novel endochin-like quinolones (ELQs) for their activity against P. knowlesi in vitro and compared this with their activity against P. falciparum tested under identical conditions. We demonstrated that ELQs are potent against P. knowlesi (50% effective concentration, <117 nM) and equally effective against P. falciparum. We then screened selected quinolones and partner drugs using a longer exposure (2.5 life cycles) and found that proguanil is 10-fold less potent against P. knowlesi than P. falciparum, while the quinolones demonstrate similar potency. Finally, we used isobologram analysis to compare combinations of the ELQs with either proguanil or atovaquone. We show that all quinolone combinations with proguanil are synergistic against P. falciparum. However, against P. knowlesi, no evidence of synergy between proguanil and the quinolones was found. Importantly, the combination of the novel quinolone ELQ-300 with atovaquone was synergistic against both species. Our data identify potentially important species differences in proguanil susceptibility and in the interaction of proguanil with quinolones and support the ongoing development of novel quinolones as potent antimalarials that target multiple species.

A cluster of dendritic cells (termed mregDCs), observed in humans and mice, restricted antitumor immunity.

Background:

The Center to Reduce Cancer Health Disparities (CRCHD), NCI, implemented Screen to Save, NCI's Colorectal Cancer Outreach and Screening Initiative to promote awareness and knowledge of colorectal cancer in racial/ethnic and rural populations.

Escherichia coli DNA Pol I can carry out three enzymatic reactions: It possesses 5' -> 3' DNA polymerase activity and 3' -> 5' and 5' -> 3' exonuclease activity. Pol I can be cleaved by mild treatment with subtilisin into two fragments; the larger fragment is known as the Klenow fragment. The Klenow fragment retains the polymerizing activity and the 3' -> 5' exonuclease of the holo-enzyme but lacks its powerful 5' -> 3' exonuclease activity. These enzymes and their applications in molecular cloning are introduced here.

The Klenow fragment, which retains the template-dependent deoxynucleotide polymerizing activity and the 3' -> 5' exonuclease of the holo-enzyme but lacks its powerful 5' -> 3' exonuclease activity, is used to fill recessed 3' termini of dsDNA. In this protocol, fragments suitable as templates for the end-filling reaction are produced by digestion of DNA with an appropriate restriction enzyme. The Klenow enzyme is then used to catalyze the attachment of dNTPs to the recessed 3'-hydroxyl groups.

Most of us will stop renting and become a homeowner one day. But how do you know when it’s a good time to buy your own home? These are four signs that you’re ready to stop renting and become a home owner.

The upsurge of land price leads to a real estate bubble, pushing up production costs, creating a virtual tendency and gradually increasing bad debt.

On May 7th, we announced a change to our brand from the eLearning Guild to the Learning Guild. As a member of our community, you […]

The post The eLearning Guild is now The Learning Guild. Here’s Why. appeared first on e-Learning Feeds.

Sony Interactive Entertainment announced Rainbow Six Siege, The Evil Within 2 and Get Even are now available for PlayStation Now subscribers. 

Rainbow Six Siege will only be available to download and stream until Monday, November 2. Downloads for the game are limited to the PlayStation 4 only. 

PlayStation Now features over 800 games on the PlayStation 4, PlayStation 3 and PlayStation 2 available to stream and play on the PlayStation 4 and Windows PC. PS4 subscribers can download and install over 300 games to play locally. 

Here is an overview of the three games:

Rainbow Six Siege

It’s the perfect time to try out the deeply tactical, always tense multiplayer shooter that has become an esports phenomenon in the years since its 2015 debut. Strained matches are built around two competing teams of players using a variety of weapons, gadgets and sound strategies to triumph over the other. While you’ll need to adapt to its uncompromising gameplay, it only makes every well-earned victory all the sweeter. Please note: this is the base edition.

The Evil Within 2

Genre aficionados will have plenty to savour with this horror sequel as you enter the alternate reality town of Union in search of your long thought dead daughter. Sickeningly brilliant creature designs and jump scares await as you explore the town’s expansive areas filled with dangers and secrets. But armed as you are with an arsenal of monster-shredding weaponry, as well as having the option to keep things low-key with a more stealthy approach, you’re ready to take on every abomination that stands between you and your family reunion.

Get Even

Puzzler, shooter, psychological thriller. Developer The Farm 51’s mashup of genres makes for an intriguing first-person adventure. Finding yourself trapped inside an insane asylum with a virtual reality device surgically grafted to your head, you’re forced to track down and ‘enter’ memories to gradually unravel the mystery behind your incarceration. As gripping as any murder mystery and with events shaped by your decisions, Get Even is a must play for those who love stories with plenty of twists.

A life-long and avid gamer, William D'Angelo was first introduced to VGChartz in 2007. After years of supporting the site, he was brought on in 2010 as a junior analyst, working his way up to lead analyst in 2012. He has expanded his involvement in the gaming community by producing content on his own YouTube channel and Twitch channel dedicated to gaming Let's Plays and tutorials. You can contact the author at wdangelo@vgchartz.com or on Twitter @TrunksWD.

Full Article - https://www.vgchartz.com/article/443391/playstation-now-adds-rainbow-six-siege-the-evil-within-ii-and-get-even/

Microsoft and Turn 10 Studios announced Forza Street is now available worldwide for free on iOS and Android.

View the launch trailer below:

Here is an overview of the game:

This all-new Forza experience lets you jump into the game for quick, under-a-minute races where you compete to unlock new cars and upgrade parts to grow your car collection. We are excited for iOS and Android players to jump into this free to play mobile experience designed to be played anytime, anywhere, and excite anyone who loves cars.

Forza Street was also designed to be an evolving experience. The game is based in a street racing world with interesting characters, mystery, and  intrigue,  explored through a narrative driven campaign, weekly Spotlight Events, and limited time Themed Events, all of which provide players an opportunity to expand their car collections. For players looking for an additional challenge, weekly Rivals events let them take their collections against other players in the community in leaderboard based asynchronous challenges.

As a special gift to everyone who plays Forza Street between today and June 5 2020, players will receive a 2017 Ford GT and added in-game credits and gold to help you unlock new cars and grow your car collection! Don’t miss out on this chance to get this rare supercar added to your garage.

For gamers joining the fun via the Samsung Galaxy Store, we have two more gifts for you: anyone who downloads through the Galaxy Store on their Samsung devices will receive the 2015 Ford Mustang GT with a custom Galaxy themed paint. If players have the latest Galaxy S20 devices, they will also receive the 2015 Chevrolet Corvette Z06 with a Custom Galaxy, and in-game credits and gold.

Want to play Forza Street across multiple device? We got you covered – Forza Street supports Xbox Live. When you sign in with Xbox Live, players will be able to unlock Xbox Achievements and transfer game progress across your Windows, iOS, and Android devices.

A life-long and avid gamer, William D'Angelo was first introduced to VGChartz in 2007. After years of supporting the site, he was brought on in 2010 as a junior analyst, working his way up to lead analyst in 2012. He has expanded his involvement in the gaming community by producing content on his own YouTube channel and Twitch channel dedicated to gaming Let's Plays and tutorials. You can contact the author at wdangelo@vgchartz.com or on Twitter @TrunksWD.

Full Article - https://www.vgchartz.com/article/443397/forza-street-now-available-for-ios-and-android/

If you didn’t feel challenged enough by Indivisible‘s main platforming and punching campaign, here’s some good news for ya. Lab Zero have released a new DLC for their turn-ish-based RPG today that promises the “forty specially crafted levels await to test your platforming and combat skills.” The Razmi’s Challenges DLC is available now, so you […]

The millennium bug is back with a vengeance, after programmers in the 1990s simply pushed the problem back by 20 years

Twitter used to be full of cat memes and had a culture of sharing. Now, I pay a company to make sure my presence on the site is extremely limited, writes Annalee Newitz

The UK government will begin trials of its coronavirus contact-tracing app this week, but what impact it will have on slowing the spread of covid-19 is unclear

TfL as in Transport for London. Greeeeeaaat.

It does make several enormous assumptions about the future that now seem perilously flaky.

Up until now mask-wearing had only been an unenforced suggestion by the company.

As a very ugly standoff develops between legacy cinemas and legacy studios, Disney appears to be playing its cards close to its chest.

Just after the prosecutor assigned to the case resigned on Thursday, the Department of Justice announced that it dropped the charges against Michael Flynn, the former national security advisor who’d already pleaded guilty to lying to the FBI about his contacts with Russia. 

President Trump forecast this before it happened. Last week, he insisted that Flynn had been exonerated.  Apparently referring to his pardon power, Trump suggested that if the court did not do something he would use “a different kind of power.”

And now it’s happened. While the president has the broad power to pardon, he should not control individual prosecutorial decisions, especially those concerning a political ally. It is extremely unusual for the government to dismiss charges after a guilty plea. This is a sign that the historic independence of the Justice Department has been compromised. 

Read more at The Daily Beast.

“HOLY MOTHER OF GOD.” 

That’s how epidemiologist Eric Feigl-Ding began a since-deleted 14-tweet thread on Jan. 25 warning about the “thermonuclear pandemic level bad” infectiousness of the coronavirus that broke out in Wuhan, China.

The first confirmed U.S. case had been announced by the Centers for Disease Control and Prevention (CDC) days earlier outside Seattle. But the disease was not widely understood to be a potentially nightmarish pandemic. Many infectious disease experts had been ignored despite warning for years that the U.S. was not prepared for a seemingly inevitable health crisis. Feigl-Ding, a visiting scientist at Harvard’s Department of Nutrition, wanted to help ensure their message was heard.

Read more at The Daily Beast.

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A tiny meteorite found in Antarctica doesn’t match any asteroid or comet we know of. Instead, it must have come from a mystery parent body that’s full of water

Extraterrestrial oceans are an obvious place to search for alien life, but getting there and having a look won't be easy, says NASA's Kevin Hand in his book Alien Oceans

Astronomers found a star that appeared to be orbiting nothing at all – but it’s actually the closest black hole ever at just 1000 light years away

Startup Cerebras has built a wafer-size chip for AI, but it isn’t the only one possible

Your weekly selection of awesome robot videos

This robot dog can scale ladders that a real dog would struggle with