In a study published in online journal npj Schizophrenia, Johns Hopkins researchers found that a history of Candida yeast infections was more common in a group of subjects with schizophrenia or bipolar disorder than in those without these disorders...

An emphasis on plant-based ingredients aligns with Pureture's commitment to environmentally friendly and sustainable food production, ensuring the health and longevity of future food sources. 

Cymbiotika, a nutritional supplement brand known for creating pure, clinically backed supplements, launched Red Yeast Rice. In the United states, Red Yeast Rice has been used as a natural alternative to statin therapy in treating people with mild to moderate hypercholesterolemia by significantly lowering LDL (“bad”) cholesterol levels, as well as total cholesterol.

Today's industrial yeast strains are used to make beer, wine, bread, biofuels, and more, but their evolutionary history is not well studied. In a Cell paper publishing September 8, researchers describe a family tree of these microbes with an emphasis on beer yeast. The resulting genetic relationships reveal clues as to when yeast was first domesticated, who the earliest beer brewers were, and how humans have shaped this organism's development.

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Ajinomoto Health & Nutrition North America on its Savorboost K yeast extract and how it works in snack applications.

Biospringer North America Corporation has announced an expansion on its yeast extract site in Cedar Rapids, Iowa, to support the growing market trend for natural origin ingredients derived from fermentation.  

Renaissance's non-GMO acrylamide-reducing yeast is a clean-label ingredient that reduces the formation of acrylamide in many common foods, including chips, crackers, bread, cereals and many other everyday foods and snacks

Grant and support from the Canadian Food Innovation Network and the Canadian Technology Accelerator program propel the commercial development of yeast expected to greatly reduce off-flavors found in plant-based proteins.

Advisory and funding support is helping to advance this environmentally safe and sustainable yeast-based RNA production and oral delivery technology to precisely target specific genes in specific organisms, while avoiding damage to other organisms.

How our billion-year-old cousin, baker’s yeast, can reveal — more reliably than leading algorithms — whether a genetic mutation is actually harmful.Toronto, ON – It’s easier than ever to sequence our DNA, but doctors still can’t exactly tell from our genomes which diseases might befall us. Professor Fritz Roth is setting out to change this by […]

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The Yeast All Around Us

Alexander S. Hebert
Jan 1, 2014; 13:339-347
Technological Innovation and Resources

Albrecht Gruhler
Mar 1, 2005; 4:310-327
Research

The HIV-1 protein Gag assembles at the plasma membrane and drives virion budding, assisted by the cellular endosomal complex required for transport (ESCRT) proteins. Two ESCRT proteins, TSG101 and ALIX, bind to the Gag C-terminal p6 peptide. TSG101 binding is important for efficient HIV-1 release, but how ESCRTs contribute to the budding process and how their activity is coordinated with Gag assembly is poorly understood. Yeast, allowing genetic manipulation that is not easily available in human cells, has been used to characterize the cellular ESCRT function. Previous work reported Gag budding from yeast spheroplasts, but Gag release was ESCRT-independent. We developed a yeast model for ESCRT-dependent Gag release. We combined yeast genetics and Gag mutational analysis with Gag-ESCRT binding studies and the characterization of Gag-plasma membrane binding and Gag release. With our system, we identified a previously unknown interaction between ESCRT proteins and the Gag N-terminal protein region. Mutations in the Gag-plasma membrane–binding matrix domain that reduced Gag-ESCRT binding increased Gag-plasma membrane binding and Gag release. ESCRT knockout mutants showed that the release enhancement was an ESCRT-dependent effect. Similarly, matrix mutation enhanced Gag release from human HEK293 cells. Release enhancement partly depended on ALIX binding to p6, although binding site mutation did not impair WT Gag release. Accordingly, the relative affinity for matrix compared with p6 in GST-pulldown experiments was higher for ALIX than for TSG101. We suggest that a transient matrix-ESCRT interaction is replaced when Gag binds to the plasma membrane. This step may activate ESCRT proteins and thereby coordinate ESCRT function with virion assembly.

Melissa Gómez
Dec 1, 2020; 61:1658-1674
Research Articles

Xanthophyllomyces dendrorhous is a basidiomycete yeast that produces carotenoids, mainly astaxanthin. Astaxanthin is an organic pigment of commercial interest due to its antioxidant and coloring properties. X. dendrorhous has a functional SREBP pathway, and the Sre1 protein is the SREBP homolog in this yeast. However, how sterol regulatory element (Sre)1 promotes the biosynthesis of sterols and carotenoids in X. dendrorhous is unknown. In this work, comparative RNA-sequencing analysis between modified X. dendrorhous strains that have an active Sre1 protein and the WT was performed to identify Sre1-dependent genes. In addition, Sre1 direct target genes were identified through ChIP combined with lambda exonuclease digestion (ChIP-exo) assays. SRE motifs were detected in the promoter regions of several Sre1 direct target genes and were consistent with the SREs described in other yeast species. Sre1 directly regulates genes related to ergosterol biosynthesis as well as genes related to the mevalonate (MVA) pathway, which synthesizes the building blocks of isoprenoids, including carotenoids. Two carotenogenic genes, crtE and crtR, were also identified as Sre1 direct target genes. Thus, carotenogenesis in X. dendrorhous is regulated by Sre1 through the regulation of the MVA pathway and the regulation of the crtE and crtR genes. As the crtR gene encodes a cytochrome P450 reductase, Sre1 regulates pathways that include cytochrome P450 enzymes, such as the biosynthesis of carotenoids and sterols. These results demonstrate that Sre1 is a sterol master regulator that is conserved in X. dendrorhous.

Maker of the “world famous buttermilk drop,” New Orleans actor Dwight Henry is expanding his baking empire

Title: Yeast infections vs. STDs in Men and Women
Category: Diseases and Conditions
Created: 1/8/2020 12:00:00 AM
Last Editorial Review: 7/29/2022 12:00:00 AM

Adding enzyme to engineered yeast boosts production of thebaine, a key morphine intermediate

Single-particle electron cryo-microscopy (cryoEM) has undergone a `resolution revolution' that makes it possible to characterize megadalton (MDa) complexes at atomic resolution without crystals. To fully exploit the new opportunities in molecular microscopy, new procedures for the cloning, expression and purification of macromolecular complexes need to be explored. Macromolecular assemblies are often unstable, and invasive construct design or inadequate purification conditions and sample-preparation methods can result in disassembly or denaturation. The structure of the 2.6 MDa yeast fatty acid synthase (FAS) has been studied by electron microscopy since the 1960s. Here, a new, streamlined protocol for the rapid production of purified yeast FAS for structure determination by high-resolution cryoEM is reported. Together with a companion protocol for preparing cryoEM specimens on a hydrophilized graphene layer, the new protocol yielded a 3.1 Å resolution map of yeast FAS from 15 000 automatically picked particles within a day. The high map quality enabled a complete atomic model of an intact fungal FAS to be built.

Ana Loncar, Sergio A. Rincon, Manuel Lera Ramirez, Anne Paoletti, and Phong T. Tran

To segregate the chromosomes faithfully during cell division, cells assemble a spindle that captures the kinetochores and pulls them towards opposite poles. Proper spindle function requires correct interplay between microtubule motors and non-motor proteins. Defects in spindle assembly or changes in spindle dynamics are associated with diseases like cancer or developmental disorders. Here we compared mitotic and meiotic spindles in fission yeast. We show that even though mitotic and meiotic spindles undergo the typical three phases of spindle elongation, they have distinct features. We found that the relative concentration of kinesin-14 Pkl1 is decreased in meiosis I compared to mitosis, while the concentration of kinesin-5 Cut7 remains constant. We identified the second kinesin-14 Klp2 and microtubule dynamics as factors necessary for proper meiotic spindle assembly. This work defines differences between mitotic and meiotic spindles in fission yeast, and provides prospect for future comparative studies.

Julian Chollet, Alexander Dünkler, Anne Bäuerle, Laura Vivero-Pol, Medhanie A. Mulaw, Thomas Gronemeyer, and Nils Johnsson

Yeast cells select the position of their new bud at the beginning of each cell cycle. The recruitment of the septins to this prospective bud site is one of the critical events in a complex assembly pathway that culminates in the outgrowth of a new daughter cell. Hereby, the septin-rods follow the high concentration of Cdc42_GTP that is generated by the focused localization of its GEF Cdc24. We show that shortly before budding Cdc24 not only activates Cdc42 but also transiently interacts with Cdc11, the septin subunit that caps both ends of the septin rods. Mutations in Cdc24 reducing the affinity to Cdc11 impair septin recruitment and decrease the stability of the polarity patch. The interaction between septins and Cdc24 thus reinforces bud assembly at sites where septin structures are formed. Once the septins polymerize into the ring, Cdc24 is found at the cortex of the bud and directs its further outgrowth from this position.

Untreated wastewater from the baker’s yeast industry can be used to irrigate crops without negatively affecting the chemical quality of the groundwater beneath, recently published research concludes. Although the wastewater increased concentrations of some groundwater contaminants in an area with a high water table, these levels would not pose a risk to human health even if this water was used for drinking.

Most women know the burning, itching signs of a yeast infection, but before reaching for an OTC medication, you might try these natural home remedies.

You may not realize that nutritional yeast is a vitamin-packed protein. Here's how to add it to salads, pastas and more.

A yeast extract containing a peptide, such as γ-Glu-X and γ-Glu-X-Gly, wherein X can represent an amino acid or an amino acid derivative other than Cys and derivatives thereof, is prepared by culturing a yeast in a medium containing a peptide such as γ-Glu-X, γ-Glu-X-Gly and X-Gly, and preparing a yeast extract from the obtained cells.

It has unexpectedly been found that Pichia spp. strains have advantageous properties useful in the beer fermentation process. In particular, Pichia spp. yeast strains can be combined with normal beer yeast strains and different hop varieties in a fermentation process to produce synergistic effects—namely, the increased production of esters in the fermentation product. More specifically, the yeast can be used to produce increased levels of isoamyl acetate, isobutyl acetate, ethyl propionate, ethyl valerate, ethyl butyrate, ethyl decanoate and ethyl octanoate in beer. In addition, the Pichia spp. strain interacts differently with different hop varieties, so the flavor profile of beer can be tuned by using different combinations of Pichia spp. strains and hops. The present invention relates to a method of brewing beer using a Pichia spp. yeast strain and at least one hop variety, a beer obtainable by such a method and use of a Pichia spp. yeast strain according to the present invention.

Scallion pancakes are a savory Chinese stovetop bread that can be a fun cooking project. They use a dough that doesn't require yeast.

SOURDOUGH BREAD is a popular recipe for those wanting to cook during the lockdown, but how can you make it without yeast?

SOURDOUGH BREAD is a popular recipe for those wanting to cook during the lockdown, but how can you make it without yeast?

Albrecht Gruhler
Mar 1, 2005; 4:310-327
Research

David Cluet
Apr 1, 2020; 19:701-715
Technological Innovation and Resources

Andreas Linden
Apr 24, 2020; 0:RA120.002028v1-mcp.RA120.002028
Research

We present a technological advancement for the estimation of the affinities of Protein-Protein Interactions (PPIs) in living cells. A novel set of vectors is introduced that enables a quantitative yeast two-hybrid system based on fluorescent fusion proteins. The vectors allow simultaneous quantification of the reaction partners (Bait and Prey) and the reporter at the single-cell level by flow cytometry. We validate the applicability of this system on a small but diverse set of PPIs (eleven protein families from six organisms) with different affinities; the dissociation constants range from 117 pm to 17 μm. After only two hours of reaction, expression of the reporter can be detected even for the weakest PPI. Through a simple gating analysis, it is possible to select only cells with identical expression levels of the reaction partners. As a result of this standardization of expression levels, the mean reporter levels directly reflect the affinities of the studied PPIs. With a set of PPIs with known affinities, it is straightforward to construct an affinity ladder that permits rapid classification of PPIs with thus far unknown affinities. Conventional software can be used for this analysis. To permit automated analysis, we provide a graphical user interface for the Python-based FlowCytometryTools package.

Keisuke Mori
Apr 29, 2020; 0:jlr.RA120000803v1-jlr.RA120000803
Research Articles

The yeast protein Mpo1 belongs to a protein family that is widely conserved in bacteria, fungi, protozoa, and plants, and is the only protein of this family whose function has so far been elucidated. Mpo1 is an Fe²⁺-dependent dioxygenase that catalyzes the α-oxidation reaction of 2-hydroxy (2-OH) long-chain FAs produced in the degradation pathway of the long-chain base phytosphingosine. However, several biochemical characteristics of Mpo1, such as its catalytic residues, membrane topology, and substrate specificity, remain unclear. Here, we report that yeast Mpo1 contains two transmembrane domains and that both its N- and C-terminal regions are exposed to the cytosol. Mutational analyses revealed that three histidine residues conserved in the Mpo1 family are especially important for Mpo1 activity, suggesting that they may be responsible for the formation of coordinate bonds with Fe²⁺. We found that, in addition to activity toward 2-OH long-chain FAs, Mpo1 also exhibits activity toward 2-OH very-long-chain FAs derived from the FA moiety of sphingolipids. These results indicate that Mpo1 is involved in the metabolism of long-chain to very-long-chain 2-OH FAs produced in different pathways. We noted that the growth of mpo1 cells is delayed upon carbon deprivation, suggesting that the Mpo1-mediated conversion of 2-OH FAs to non-hydroxy FAs is important for utilizing 2-OH FAs as a carbon source under carbon starvation. Our findings help to elucidate the as-yet-unknown functions and activities of other Mpo1 family members.

Protein cross-linking and the analysis of cross-linked peptides by mass spectrometry is currently receiving much attention. Not only is this approach applied to isolated complexes to provide information about spatial arrangements of proteins but it is also increasingly applied to entire cells and their organelles. As in quantitative proteomics, the application of isotopic labelling further makes it possible to monitor quantitative changes in the protein-protein interactions between different states of a system. Here, we cross-linked mitochondria from Saccharomyces cerevisiae grown on either glycerol- or glucose-containing medium to monitor protein-protein interactions under non-fermentative and fermentative conditions. We investigated qualitatively the protein-protein interactions of the 400 most abundant proteins applying stringent data-filtering criteria, i.e. a minimum of two cross-linked peptide spectrum matches and a cut-off in the spectrum scoring of the used search engine. The cross-linker BS3 proved to be equally suited for connecting proteins in all compartments of mitochondria when compared with its water-insoluble but membrane-permeable derivative DSS. We also applied quantitative cross-linking to mitochondria of both the growth conditions using stable-isotope labelled BS3. Significant differences of cross-linked proteins under glycerol and glucose conditions were detected, however, mainly due to the different copy numbers of these proteins in mitochondria under both the conditions. Results obtained from the glycerol condition indicate that the internal NADH:ubiquinone oxidoreductase Ndi1 is part of an electron transport chain supercomplex. We have also detected several hitherto uncharacterized proteins and identified their interaction partners. Among those, Min8 was found to be associated with cytochrome c oxidase. BN-PAGE analyses of min8 mitochondria suggest that Min8 promotes the incorporation of Cox12 into cytochrome c oxidase.

Xanthophyllomyces dendrorhous is a basidiomycete yeast known as a natural producer of astaxanthin, a carotenoid of commercial interest because of its antioxidant properties. Recent studies indicated that X. dendrorhous has a functional SREBP pathway involved in the regulation of isoprenoid compound biosynthesis, which includes ergosterol and carotenoids. SREBP is a major regulator of sterol metabolism and homeostasis in mammals; characterization in fungi also provides information about its role in the hypoxia adaptation response and virulence. SREBP protease processing is required to activate SREBP pathway functions in fungi. Here, we identified and described the STP1 gene, which encodes a metallopeptidase of the M50 family involved in the proteolytic activation of the transcription factor Sre1 of the SREBP pathway, in X. dendrorhous. We assessed STP1 function in stp1 strains derived from the wild-type and a mutant of ergosterol biosynthesis that overproduces carotenoids and sterols. Bioinformatic analysis of the deduced protein predicted the presence of characteristic features identified in homologs from mammals and fungi. The stp1 mutation decreased yeast growth in the presence of azole drugs and reduced transcript levels of Sre1-dependent genes. This mutation also negatively affected the carotenoid- and sterol-overproducing phenotype. Western blot analysis demonstrated that Sre1 was activated in the yeast ergosterol biosynthesis mutant and that the stp1 mutation introduced in this strain prevented Sre1 proteolytic activation. Overall, our results demonstrate that STP1 encodes a metallopeptidase involved in proteolytic activation of Sre1 in X. dendrorhous, contributing to our understanding of fungal SREBP pathways.

Manogepix (APX001A) is the active moiety of the novel drug candidate fosmanogepix (APX001). We previously reported the broad-spectrum activity of manogepix but also observed a correlation between increased manogepix and fluconazole MICs. Here we extended this study and included isolates with acquired fluconazole resistance.

Isolates (n=835) were identified using CHROMagar, MALDI-TOF and, when needed, ITS-sequencing. EUCAST E.Def 7.3.1 susceptibility testing included manogepix, amphotericin B, anidulafungin, micafungin, fluconazole and voriconazole. Manogepix wildtype-upper-limit (WT-UL) values were established following EUCAST-principles for ECOFF setting allowing wildtype/non-wildtype classification. Drug-specific MIC correlations were investigated using Pearson's correlation.

Manogepix modal MICs were low (range 0.004-0.06 mg/L against 16/20 included species). Exceptions were C. krusei and C. inconspicua, and to a lesser extent C. kefyr and Pichia kluyveri. The activity was independent of Fks echinocandin hot-spot alterations (n=17). Adopting the WT-UL established for C. albicans, C. dubliniensis, C. glabrata, C. parapsilosis and C. tropicalis, 14/724 (1.9%) isolates were non-wildtype for manogepix. Twelve of these (85.7%) were also non-wildtype for fluconazole. A statistically significant correlation was observed between manogepix and fluconazole MICs for C. albicans, C. dubliniensis, C. glabrata, C. parapsilosis and C. tropicalis (Pearson r=0.401-0.575), but not between manogepix and micafungin or amphotericin B MICs for any species except C. tropicalis (r=0.519 for manogepix versus micafungin).

Broad-spectrum activity was confirmed for manogepix against contemporary yeast. However, a 1-4 two-fold-dilution increase in manogepix MICs is observed in a subset of isolates with acquired fluconazole resistance. Further studies on the potential underlying mechanism and implication for optimal dosing are warranted.

By Sr. Joan L. Roccasalvo, C.S.J.

It’s a wonderful phenomenon—yeast.  It permeates lifeless flour and causes it to rise and expand.  The power of yeast effects the brewing of beer and the making of wine.   The yeast plant is a fungus that grows without limits to its borders.  Only if yeast is alive and active will it interact with the dough.

On her TV program, “Martha Bakes,” the talented Ms. Stewart cannot contain her delight when she makes yeast dough: “Look at the sheen—so soft and shiny! The aroma is “bee-you-tee-ful,” and the fragrance gratifies all the senses!” Follow these instructions: proof active yeast, blend it into the flour mixture, and let it rise to double the size.  From yeast dough come baked goods such as breads, sticky buns and sugar buns, and monkey bread.  “Soo pretty, soo delicious,” Ms. Stewart swoons over her culinary works of art.

Yeast as a Metaphor

In the Matthean parable (13:33), the reign of God is like yeast that a woman took and kneaded into three measures of flour.  Eventually the entire mass of dough began to rise.  The image of yeast was a favorite in the Early Church.  Everyone understood the inner power of yeast with its limitless ability to make things grow, even in small beginnings with “three measures of flour.”  They grasped the comparison.  The yeast referred to the Church as an unlimited and growing reality, “destined ultimately to be present everywhere and to affect everything, though by no means to convert everything into itself” (Walter J. Ong, “Yeast: A Parable for Catholic Higher Education,” America Magazine, April 7, 1990).  The Church is catholic because it has always been expanding into new and shiny ‘dough’ without limit. Katholicos, from kata or kath and holos, means “through-the-whole or “throughout-the-whole.”

The Laity: Worldly and Yet Unworldly    

The laity are catholic, yeast in business and finance, entertainment, nursing and medicine, arts and science, law and law enforcement, politics, and sports.  They are the inner power with its limitless ability to make things grow, even in small ways. The laity find their holiness in the world with its financial concerns and family responsibilities.  Those who marry and have children become not just a family but also the Domestic Church.

In 1987, the Catholic Church held a World Synod on the Laity, one of many, beginning with Vatican II in the 1960s.  According to the synod’s final document, the laity are equal with clergy and consecrated religious in the life and mission of the Church.  

The call to holiness of the laity differs from the vocation of consecrated religious.  The laity are to be in the world in an unworldly way.  They approach life with wisdom that teaches the limited and relative value of material things. This would seem to be a contradiction in terms.  How to be worldly and unworldly at the same time?   It cannot be easy, for at times, the challenges seem insurmountable.  Yet, it remains for the lay vocation to find a theology of being present in the world. It is a practical spirituality of the family and the workplace.  For the laity, this is where holiness resides.*  

Holiness of the Laity

The holiness of the laity began with Jesus himself.  He was a rabbi and teacher, as were his disciples. Peter was a married man, and for all we know, so were the other apostles, the exception being John, the Beloved Disciple.  

St. Paul addresses and refers to those he evangelized as ‘saints,’ meaning that they were on their way to becoming saints.  In the Early Church, there were no consecrated institutes of men and women.  All Christians grasped the importance of living as disciples and ambassadors of the Lord.

As increasing numbers of Christians came to view the world as wicked, they flocked to the desert to live alone. When the desert grew so overcrowded with these solitaries, they came together and formed religious communities.  Thus, the start of monastic orders of men and women.

Prayer

Consecrated men and women, and especially those who live in cloisters, spend several hours a day in prayer.

This is not the way of the laity. Their days focus almost entirely on family and the means of supporting it.  Their prayer is measured not in hours but in minutes—two here, five there, perhaps a Holy Hour or Retreat Day on rare occasions.

The conciliar document on the sacred liturgy encourages Catholic families to pray portions of the Liturgy of the Hours (#102-111).  The Hours are not private or devotional prayer but the prayer of the entire Church, the Church at prayer.  Praying the psalms nourishes Catholic family life whose welfare is daily beset with conflicting external forces. If prayer is the underlying power of strong family life, then parents can find ways to incorporate parts of the Hours into their daily schedule. In prayer, married couples derive the strength of God’s grace to live their married vocation.  

As children mature, they too must learn to travel the road to discipleship in the Lord.  Small children can be taught to pray a psalm or two at bed time. If this is not feasible during the week, then prayer on weekend is an alternate possibility.  

A minimal and external Christianity will not fortify today’s Domestic Church but only a vibrant Christianity in which Christ is a living reality.  It takes a few minutes to pray short sections of the Hours, even on public transit.  It is a consoling thought to recall that “in him, we live and move and have our being” (Acts 17:28).  

At Pre-Cana instructions, couples can learn the practice of making the Hours an integral part of their married life.

Can Yeast Corrupt?

The image of yeast is not always positive.  In First Corinthians 5:6-8, St. Paul mentions what all Jews understood.  At the Paschal festival time, they were to destroy all yeasted products because leaven was a metaphor for the corruptive influence of evil, for puffing up the self, leaving no room for God.   

Proofing the yeast in warm water will yield bubbles around the surface, and the yeast will become puffed up if it does not interact with the flour dough.  The puffed up yeast will die.  In this sense, neither the laity, nor any minister in the Church, can afford to be puffed up with pride.

Élisabeth Leseur (1866-1914) and Félix Leseur (1861-1950)

The story of Élisabeth Arrighi Leseur exemplifies the limitless power of marital love.  Élisabeth was born into a wealthy French Catholic family of Corsican descent.  As a child, she had contracted hepatitis, a disease from which she suffered all her life.  At twenty-one, she met Félix Leseur, a medical doctor, who also came from an affluent Catholic family.  Shortly before they were to be married, Élisabeth discovered that Félix was no longer a practicing Catholic.  Soon he became well known as the editor of an anti-clerical, atheistic newspaper.  

Despite the circumstances, the couple married, for Élisabeth was deeply in love with Félix.  They were unable to have children, a fact that made their marriage all the more difficult.  His attack on her religious devotion prompted an even more serious fidelity to the faith. She bore the brunt of his hatred of the Church with patient love.  At thirty-two years of age, Élisabeth experienced the grace to a deeper form of prayer.  She was convinced that her task now was to love her husband and pray for his conversion while remaining steadfast during his taunts against religion, and the Church in particular.

Homebound and Bed-Ridden

Élisabeth’s deteriorating health forced her to lead a sedentary life.  She received visitors and was able to conduct a vibrant apostolate from the confines of her home.  She became a devotee of St. Francis de Sales who wrote for the layperson in the seventeenth century. His Introduction to the Devout Life, perhaps the most famous spiritual guide of all time, is an offshoot of the Ignatian Exercises. During this period, Élisabeth kept a secret spiritual diary.  

When, at the age of forty-five, Élisabeth underwent surgery and radiation for the removal of a malignant tumor, she recovered and continued to receive visitors to her home. Three years later, she succumbed to cancer.  Her life has been recommended for sainthood. Why?  We turn the page to continue the narrative of her husband.

Dr. Félix Leseur

After Élisabeth’s death, Félix found a note addressed to him.  Not only did it predict his conversion, but he would also become a Dominican priest.  His hatred of the Church prompted him to expose her note as a fake, and he decided to do so at Lourdes, the famous Marian shrine in France.  There, something prevented him from carrying out his intended project—call it God’s intervening grace. As Élisabeth had predicted, he experienced a conversion and published her spiritual journal.  In 1919, Félix entered the Dominican Order, was ordained a priest four years later, and spent his remaining years speaking about his wife’s difficult yet remarkable life with him.  

In 1924, the future Archibishop Fulton J. Sheen made a retreat under Fr. Leseur’s direction.  It was at this time that he learned of Élisabeth’s life and her husband’s conversion.  In 1934, Fr. Leseur, O.P. worked to begin the cause for her canonization, and the Archbishop shared the story of this remarkable married couple in many presentations.  Élisabeth is currently a Servant of God, the first step in the cause for sainthood.

Élisabeth Leseur’s suffering was not wasted. On the contrary, her lifelong devotion to Félix was central to his conversion.  She became the yeast that permeated the lifeless soul of her husband.  It forever transformed his life so that he could affect change in the lives of others. Love begets love.

*The Ignatian “Prayer for Finding God in All Things” by Joan L. Roccasalvo, C.S.J. can help the busy person find God throughout the day.  Copies are available from the Institute of Jesuit Sources, Boston, MA.

Title: Drug for Yeast Infections May Raise Miscarriage Risk, FDA Warns
Category: Health News
Created: 4/26/2016 12:00:00 AM
Last Editorial Review: 4/27/2016 12:00:00 AM

Lactoferrin (LF) is a multifunctional milk protein with antimicrobial activity against a range of pathogens. While numerous studies report that LF is active against fungi, there are considerable differences in the level of antifungal activity and the capacity of LF to interact with other drugs. Here we undertook a comprehensive evaluation of the antifungal spectrum of activity of three defined sources of LF across 22 yeast and 24 mold species and assessed its interactions with six widely used antifungal drugs. LF was broadly and consistently active against all yeast species tested (MICs, 8 to 64 μg/ml), with the extent of activity being strongly affected by iron saturation. LF was synergistic with amphotericin B (AMB) against 19 out of 22 yeast species tested, and synergy was unaffected by iron saturation but was affected by the extent of LF digestion. LF-AMB combination therapy significantly prolonged the survival of Galleria mellonella wax moth larvae infected with Candida albicans or Cryptococcus neoformans and decreased the fungal burden 12- to 25-fold. Evidence that LF directly interacts with the fungal cell surface was seen via scanning electron microscopy, which showed pore formation, hyphal thinning, and major cell collapse in response to LF-AMB synergy. Important virulence mechanisms were disrupted by LF-AMB treatment, which significantly prevented biofilms in C. albicans and C. glabrata, inhibited hyphal development in C. albicans, and reduced cell and capsule size and phenotypic diversity in Cryptococcus. Our results demonstrate the potential of LF-AMB as an antifungal treatment that is broadly synergistic against important yeast pathogens, with the synergy being attributed to the presence of one or more LF peptides.

If both can make a dough rise, why does your dough recipe call for both?

A breakthrough in controlling yeast with computers gives a glimpse at where science is headed with controlling lifeforms.