“Lord, I believe; help my unbelief!” The father of the young man in today’s gospel lesson cried out these words with tears in response to the Lord’s statement that “all things are possible to him who believes.” The father in this passage provides a good example of how we should respond to the spiritual challenges posed by our current public health crisis.

A representational image of an alligator. — AFP/file

A large alligator wandering among the planes on the tarmac at a Florida airport was captured on video by a passenger on a moving plane.

The animal, estimated by witnesses to be about 10-feet long, was seen making its way...

Washington — The Bureau of Safety and Environmental Enforcement has issued a proposed rule that would codify existing agency practice concerning the review and approval of projects proposing to use “new or unusual technology.”

Washington — A new Bureau of Safety and Environmental Enforcement final rule codifies existing agency practice related to the review and approval of projects that will feature “new or unusual technology” – including equipment used in high-pressure or high-temperature environments.

[Science] :
Korea is experiencing an unusually hot September, with thermometers reaching up to 38 degrees Celsius in Gokseong, South Jeolla Province, and Jinju, South Gyeongsang Province, on Tuesday.  Temperatures in most regions in the south and inland areas of the Chungcheong provinces, including Gwangju; Namwon, ...

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Grey reflects on his recent Greycation while grappling with chaos. Myke is keen to explore a major update to Grey's mailing list. Additionally, they discuss how to determine if a change in business is critical.

Looking through the mind of an unconventional genius, the user delves into the fascinating hidden worlds of numbers, connective logic, belief, and possibility

An Arizona man who once struggled with math in high school, has solved one of the most notorious problems in history, (The Collatz Conjecture). Thought by many to be unsolvable for decades, his accomplishment has profound implications.

The Wyoming Supreme Court ruled that a man's heart attack on his third day of work was not due to clearly unusual or abnormal employment stress for those in his…

Inability to belch syndrome is real, and doctors have an effective way to treat it.

Inability to belch syndrome is real, and doctors have an effective way to treat it.

Inability to belch syndrome is real, and doctors have an effective way to treat it.

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The Florida Roofing Contractors Association urged state regulators to freeze workers' comp rates, citing post-hurricane labor shortages and risks from inexperienced workers despite proposed cuts.

[Updated] Friday’s sitting of the House of Assembly saw a number of unusual “what if” questions posed, including queries pertaining to being kicked out of a church for stealing while committing adultery, being beaten up after sleeping with a neighbour’s wife and buying marijuana. This exchange followed a motion brought by Independent MP Terry Lister […]

The post Unusual Residents of Saint-Petersburg appeared first on English Russia.

Hurricanes Helene and Milton have bookended a particularly stormy period. What's behind it?

The main protease (3CL Mpro) from SARS–CoV-2, the etiological agent of COVID-19, is an essential enzyme for viral replication. 3CL Mpro possesses an unusual catalytic dyad composed of Cys145 and His41 residues. A critical question in the field has been what the protonation states of the ionizable residues in the substrate-binding active-site cavity are; resolving this point would help understand the catalytic details of the enzyme and inform rational drug development against this pernicious virus. Here, we present the room-temperature neutron structure of 3CL Mpro, which allowed direct determination of hydrogen atom positions and, hence, protonation states in the protease. We observe that the catalytic site natively adopts a zwitterionic reactive form in which Cys145 is in the negatively charged thiolate state and His41 is doubly protonated and positively charged, instead of the neutral unreactive state usually envisaged. The neutron structure also identified the protonation states, and thus electrical charges, of all other amino acid residues and revealed intricate hydrogen-bonding networks in the active-site cavity and at the dimer interface. The fine atomic details present in this structure were made possible by the unique scattering properties of the neutron, which is an ideal probe for locating hydrogen positions and experimentally determining protonation states at near-physiological temperature. Our observations provide critical information for structure-assisted and computational drug design, allowing precise tailoring of inhibitors to the enzyme's electrostatic environment.

Junhwan Kim
Dec 1, 2020; 61:1707-1719
Research Articles

Phospholipids, including ether phospholipids, are composed of numerous isomeric and isobaric species that have the same backbone and acyl chains. This structural resemblance results in similar fragmentation patterns by collision-induced dissociation of phospholipids regardless of class, yielding complicated MS/MS spectra when isobaric species are analyzed together. Furthermore, the presence of isobaric species can lead to misassignment of species when made solely based on their molecular weights. In this study, we used normal-phase HPLC for ESI-MS/MS analysis of phospholipids from bovine heart mitochondria. Class separation by HPLC eliminates chances for misidentification of isobaric species from different classes of phospholipids. Chromatography yields simple MS/MS spectra without interference from isobaric species, allowing clear identification of peaks corresponding to fragmented ions containing monoacylglycerol backbone derived from losing one acyl chain. Using these fragmented ions, we characterized individual and isomeric species in each class of mitochondrial phospholipids, including unusual species, such as PS, containing an ether linkage and species containing odd-numbered acyl chains in cardiolipin, PS, PI, and PG. We also characterized monolysocardiolipin and dilysocardiolipin, the least abundant but nevertheless important mitochondrial phospholipids. The results clearly show the power of HPLC-MS/MS for identification and characterization of phospholipids, including minor species.

The annual award ceremony featured costumes, songs and paper airplanes as scientists recognized comedic research across ten disciplines

Chris Martin has superstitious belief about his deathColdplay frontman, Chris Martin seems to have a superstitious mindset as he fears that he might die in a plane crash one day just like American singer and songwriter, Buddy Holly. Due to this anxiety, Martin has adopted a very unique habit of...

Voyager 2 flew by Uranus in 1986, giving us our only up-close look at the planet – but unusual space weather just before the craft arrived has given us a misleading idea about the planet’s magnetic field

During B-cell development, cells progress through multiple developmental stages, with the pro-B-cell stage defining commitment to the B-cell lineage. YY1 is a ubiquitous transcription factor that is capable of both activation and repression functions. We found here that knockout of YY1 at the pro-B-cell stage eliminates B lineage commitment. YY1 knockout pro-B cells can generate T lineage cells in vitro using the OP9-DL4 feeder system and in vivo after injection into sublethally irradiated Rag1^–/– mice. These T lineage-like cells lose their B lineage transcript profile and gain a T-cell lineage profile. Single-cell RNA-seq experiments showed that as YY1 knockout pro-B cells transition into T lineage cells in vitro, various cell clusters adopt transcript profiles representing a multiplicity of hematopoietic lineages, indicating unusual lineage plasticity. In addition, YY1 KO pro-B cells in vivo can give rise to other hematopoietic lineages in vivo. Evaluation of RNA-seq, scRNA-seq, ChIP-seq, and scATAC-seq data indicates that YY1 controls numerous chromatin-modifying proteins leading to increased accessibility of alternative lineage genes in YY1 knockout pro-B cells. Given the ubiquitous nature of YY1 and its dual activation and repression functions, YY1 may regulate commitment in multiple cell lineages.

Voyager 2 flew by Uranus in 1986, giving us our only up-close look at the planet – but unusual space weather just before the craft arrived has given us a misleading idea about the planet’s magnetic field

There are not many usable precedents for what J. D. Vance can do with a one-term vice presidency.

ISLAMABAD — The International Monetary Fund's Pakistan mission chief Nathan Porter on Tuesday (Nov 12) opened unusual talks with Pakistan over a US$7 billion (S$9.4 billion) bailout approved by its board in September, the finance ministry and sources said. The unscheduled visit of the IMF mission and talks beginning with meeting the country's finance team are too early for first review of the IMF's Extended Fund Facility (EFF), which is due in the first quarter of 2025. The chiefs of Pakistan's central bank and federal board of revenue also attended the meeting besides other officials from both the sides, the statement said. The ministry and the IMF have not officially released details of the visit. Sources in the finance ministry said the Nov 11-15 visit will discuss recent developments and programme performance to date, adding the mission was not part of the first review. The sources declined to be identified as they were not authorised to speak with the media. Pakistan has been struggling with boom-and-bust economic cycles for decades, leading to 23 IMF bailouts since 1958.

Voyager 2 flew by Uranus in 1986, giving us our only up-close look at the planet – but unusual space weather just before the craft arrived has given us a misleading idea about the planet’s magnetic field

Some 44 years ago, people found a cabbage plant with a natural mutation that destroyed the plant’s ability to make pollen.

At home in the tropical forests of Central and South America, vampire bats feed on various animals, including tapirs, mountain lions, penguins and livestock.

Watch out! Anatolian Tigers on the prowl.

As the masses have adopted RSS as a more mainstream communication medium, Web 2.0 businesses and publishers have adopted unique and unusual tools to manipulate RSS content. Here are just some of the various RSS tools that are currently available...

Unusual RSS Tools

Out-of-the-box ideas to spruce up your home

Dear Experts Pranam!

My dad has been puchased a land with a small old house (its very old, so may not required consider as a house - or in a demolishing stage) in Kottayam, Kerala, 10+ years back from a person, named as Mr.SELLER and has been registered at the concerned Sub Reg. Office (SRO). And till the last year there is no issue on this and since its a land we will do frequent visit over there to check the fencing etc. are ok and whether it is secured/safe. But last year when we tried to make an attempt to sell and tried find a buyer for the same through some local persons BUT got a news that it is in a despute and some legal issues are there in the court which is surprised us. And after an enquiry we met a person named as Mr.SON who is filed the case on this land. The case was against his Mrs.MOTHER stating that she does not have any legal rights to sell the land to the so called Mr.SELLER (as above) since that land originaaly owned by her husband named as Mr. HUSBAND (he is already expired) and his two siblings/heirs - one sister and a brother. What we understand and told by Mr.SON is, he filed the case in 2014 and now in 2019 mid, as per the court order the case has been won by him and the property will be attached to him and the above siblings. Please be noted that the case was only between him and his mother. Unfortunately his mother also expired prior to the case finals, i,e. his winning.

We have consulted with a legal adviser and got a temperory stay/injection (saying that Mr.SON and siblings been interuupting/disturbing us from staying/residing at our property, so please do the needful to stop their torturing) for the immediate Attachment and so the court has been sent notices to Mr.SON and his siblings/heirs to let the hon.court know about their statements or sayings about this issues by producing their valid documents. THere are 6 persons from the siblings side in this case other than Mr.SON. 2 of them not interested to receive the notice and so become ex.pat. Other three persons addresses are not reachable and so repeated notices two times sent again. Mr. SON was escaping each time by not receving the Notice from Ameen and in the third time he informed the Ameen that he will not receive the notice and Ameen has been noted the info and communicatd to the Hon,Court on the same. THe most important part of this is other than Mr. SON, no one including the above said siblings are not interested in this case but it is by the compulsion of Mr. SON only did involve. by the meantime very unfortunately Mr.SON is expired, recently. He is having wife and two kids.

In this scenario now we are not much interested to drag this case further and wish to make a out of court settlement if possible without any legal issues. is it possible otherwise what else are the BEST and immediate Resulting solutions in this case. I am asking this to know about your opinions in this case please.

Thanking you  in advance, Regards

Krishnan 

The former Bulgaria international recalled a League Cup game against Leeds where he played in defence for Sir Alex Ferguson's team

B.C. Provincial Health Officer Dr. Bonnie Henry announced 15 new coronavirus cases in the province on Saturday, bringing the current total to 2,330.

While there is some a general uniformity and standardisation when it comes to roadside instructions, some European countries have signs that are unique from any other nation.

Paningning, a shih tzu, sleeps flat on her back like a human and often drifts off in unusual places. Owner Janess Cua from the Philippines shared photos of the puppy which have since gone viral.

More than 40 artists from four cities -- Delhi, Mumbai, Navi Mumbai, Pune -- gathered in Mumbra, a township near Mumbai.The artists joined women in Mumbra protesting the Citizens (Amendment) Act.

The title compound [systematic name: (1R*, 8S)-2-acetamidoocta­hydro­pyrrol­izin-4-ium chloride–N-[(1R, 8S)-hexa­hydro-1H-pyrrolizin-2-yl)acetamide (1/1)], 2(C9H16N2O)·HCl or C9H17N2O+·Cl−·C9H16N2O, arose as an unexpected product when 1-exo-acetamido­pyrrolizidine (AcAP; C9H16N2O) was dissolved in CHCl3. Within the AcAP pyrrolizidine group, the unsubstituted five-membered ring is disordered over two orientations in a 0.897 (5):0.103 (5) ratio. Two AcAP mol­ecules related by a crystallographic twofold axis link to H+ and Cl− ions lying on the rotation axis, thereby forming N—H⋯N and N—H⋯Cl⋯H—N hydrogen bonds. The first of these has an unusually short N⋯N separation of 2.616 (2) Å: refinement of different models against the present data set could not distinguish between a symmetrical hydrogen bond (H atom lying on the twofold axis and equidistant from the N atoms) or static or dynamic disorder models (i.e. N—H⋯N + N⋯H—N). Computational studies suggest that the disorder model is slightly more stable, but the energy difference is very small.

One of the most challenging aspects of this research is simply studying these insects as they are falling, says Yanoviak, a tropical arthropod ecologist at the University of Arkansas, Little Rock. Small body size, rapid descent, and the long distances that they can fall, make accurate data taking a challenge.

The post Lofty experiments with gliding ants reveals secrets of their unusual flight appeared first on Smithsonian Insider.

NYT and Google teamed up to find the most distinctive Thanksgiving dishes by state. Some of the more unusual ones have whipped topping in common.

The NOAA suspects the infectious morbillivirus is responsible for the unusually high number of dolphin strandings and deaths this month.