The now former Archbishop of Canterbury Justin Welby should have taken additional steps to ensure that a church leader who left the UK suddenly could not continue his abuse in South Africa, a report suggests.

A California teenager accused of being a "serial swatter" faces up to 20 years in prison for making interstate threats after pleading guilty in federal court on Wednesday.

Iran’s state media say authorities have executed in public a man convicted of raping dozens of women over two decades

A silent crisis of ‘serial returns’ is eroding retail profit margins as uncovered in the Annual Returns Benchmark Report 2024 conducted by returns specialists ZigZag, in partnership with Retail Economics.

The company behind the hit true-crime podcast Serial is exploring a sale, according to a person familiar with the matter, putting one of digital audios biggest brands on the market as the medium becomes increasingly popular.

complete article

New York Times Co. said it would acquire Serial Productions, the maker of the hit podcast Serial, a deal that aims to further the newspapers podcasting ambitions.

Terms of the deals were not disclosed, but the transaction could be worth as much as $50 million depending on milestones and performance metrics, a person familiar with the matter said.

Not every serial killer should necessarily be either a social outcast or a sociopath. Some of them are good at disguise and may at times have a reputation of exemplary society members. Biographies of many famous serial killers of the USSR era testify to this. Chikatilo helped to catch himself Andrey Chikatilo, a serial killer from Rostov, committed his first crime in 1973, the last in 1990. Chikatilo killed 53 women and children in all that time. All the crimes were sexually motivated; he would stab and dismember his victims' bodies.

The Kemerovo regional court ruled to send Alexander Spesivtsev, a resident of Novokuznetsk, to compulsory treatment, the regional Office of the Public Prosecutor said. The defendant, Alexander Spesivtsev, is a serial killer, who killed at least 34 victims and ate them during the 1990s. Spesivtsev will undergo treatment at a special psychiatric hospital.

Snyder law enforcement officials last week arrested Daniel Allen Jr.

A silent crisis of ‘serial returns’ is eroding retail profit margins as uncovered in the Annual Returns Benchmark Report 2024* conducted by returns specialist ZigZag, in partnership with Retail Economics, accounting for 1 in 10 (11% of) online shoppers that make returns, are generating a quarter (24%) of all online non-food returns.

Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is the enzyme responsible for the first step of carbon dioxide (CO2) fixation in plants, which proceeds via the carboxylation of ribulose 1,5-biphosphate. Because of the enormous importance of this reaction in agriculture and the environment, there is considerable interest in the mechanism of fixation of CO2 by RuBisCO. Here, a serial synchrotron crystallography structure of spinach RuBisCO is reported at 2.3 Å resolution. This structure is consistent with earlier single-crystal X-ray structures of this enzyme and the results are a good starting point for a further push towards time-resolved serial synchrotron crystallography in order to better understand the mechanism of the reaction.

Fixed targets (`chips') offer efficient, high-throughput microcrystal delivery for serial crystallography at synchrotrons and X-ray free-electron lasers (XFELs). Within this family, sheet-on-sheet (SOS) chips offer noteworthy advantages in cost, adaptability, universality and ease of crystal loading. We describe our latest generation of SOS devices, which are now in active use at both synchrotrons and XFELs.

Serial crystallography (SX) efficiently distributes over many crystals the radiation dose absorbed during diffraction data acquisition, enabling structure determination of samples at ambient temperature. SX relies on the rapid and reliable replacement of X-ray-exposed crystals with fresh crystals at a rate commensurate with the data acquisition rate. `Solid supports', also known as `fixed targets' or `chips', offer one approach. These are microscopically thin solid panes into or onto which crystals are deposited to be individually interrogated by an X-ray beam. Solid supports are generally patterned using photolithography methods to produce a regular array of features that trap single crystals. A simpler and less expensive alternative is to merely sandwich the microcrystals between two unpatterned X-ray-transparent polymer sheets. Known as sheet-on-sheet (SOS) chips, these offer significantly more versatility. SOS chips place no constraint on the size or size distribution of the microcrystals or their growth conditions. Crystals ranging from true nanocrystals up to microcrystals can be investigated, as can crystals grown in media ranging from low viscosity (aqueous solution) up to high viscosity (such as lipidic cubic phase). Here, we describe our two SOS devices. The first is a compact and lightweight version designed specifically for synchrotron use. It incorporates a standard SPINE-type magnetic base for mounting on a conventional macromolecular crystallography goniometer. The second and larger chip is intended for both X-ray free-electron laser and synchrotron use and is fully compatible with the fast-scanning XY-raster stages developed for data collection with patterned chips.

Serial crystallography, born from groundbreaking experiments at the Linac Coherent Light Source in 2009, has evolved into a pivotal technique in structural biology. Initially pioneered at X-ray free-electron laser facilities, it has now expanded to synchrotron-radiation facilities globally, with dedicated experimental stations enhancing its accessibility. This review gives an overview of current developments in serial crystallography, emphasizing recent results in time-resolved crystallography, and discussing challenges and shortcomings.

Serial electron diffraction (SerialED), which applies a snapshot data acquisition strategy for each crystal, was introduced to tackle the problem of radiation damage in the structure determination of beam-sensitive materials by three-dimensional electron diffraction (3DED). The snapshot data acquisition in SerialED can be realized using both transmission and scanning transmission electron microscopes (TEM/STEM). However, the current SerialED workflow based on STEM setups requires special external devices and software, which limits broader adoption. Here, we present a simplified experimental implementation of STEM-based SerialED on Thermo Fisher Scientific STEMs using common proprietary software interfaced through Python scripts to automate data collection. Specifically, we utilize TEM Imaging and Analysis (TIA) scripting and TEM scripting to access the STEM functionalities of the microscope, and DigitalMicrograph scripting to control the camera for snapshot data acquisition. Data analysis adapts the existing workflow using the software CrystFEL, which was developed for serial X-ray crystallography. Our workflow for STEM SerialED can be used on any Gatan or Thermo Fisher Scientific camera. We apply this workflow to collect high-resolution STEM SerialED data from two aluminosilicate zeolites, zeolite Y and ZSM-25. We demonstrate, for the first time, ab initio structure determination through direct methods using STEM SerialED data. Zeolite Y is relatively stable under the electron beam, and STEM SerialED data extend to 0.60 Å. We show that the structural model obtained using STEM SerialED data merged from 358 crystals is nearly identical to that using continuous rotation electron diffraction data from one crystal. This demonstrates that accurate structures can be obtained from STEM SerialED. Zeolite ZSM-25 is very beam-sensitive and has a complex structure. We show that STEM SerialED greatly improves the data resolution of ZSM-25, compared with serial rotation electron diffraction (SerialRED), from 1.50 to 0.90 Å. This allows, for the first time, the use of standard phasing methods, such as direct methods, for the ab initio structure determination of ZSM-25.

Serial crystallography (SX) has become an established technique for protein structure determination, especially when dealing with small or radiation-sensitive crystals and investigating fast or irreversible protein dynamics. The advent of newly developed multi-megapixel X-ray area detectors, capable of capturing over 1000 images per second, has brought about substantial benefits. However, this advancement also entails a notable increase in the volume of collected data. Today, up to 2 PB of data per experiment could be easily obtained under efficient operating conditions. The combined costs associated with storing data from multiple experiments provide a compelling incentive to develop strategies that effectively reduce the amount of data stored on disk while maintaining the quality of scientific outcomes. Lossless data-compression methods are designed to preserve the information content of the data but often struggle to achieve a high compression ratio when applied to experimental data that contain noise. Conversely, lossy compression methods offer the potential to greatly reduce the data volume. Nonetheless, it is vital to thoroughly assess the impact of data quality and scientific outcomes when employing lossy compression, as it inherently involves discarding information. The evaluation of lossy compression effects on data requires proper data quality metrics. In our research, we assess various approaches for both lossless and lossy compression techniques applied to SX data, and equally importantly, we describe metrics suitable for evaluating SX data quality.

Serial crystallography requires large numbers of microcrystals and robust strategies to rapidly apply substrates to initiate reactions in time-resolved studies. Here, we report the use of droplet miniaturization for the controlled production of uniform crystals, providing an avenue for controlled substrate addition and synchronous reaction initiation. The approach was evaluated using two enzymatic systems, yielding 3 µm crystals of lysozyme and 2 µm crystals of Pdx1, an Arabidopsis enzyme involved in vitamin B6 biosynthesis. A seeding strategy was used to overcome the improbability of Pdx1 nucleation occurring with diminishing droplet volumes. Convection within droplets was exploited for rapid crystal mixing with ligands. Mixing times of <2 ms were achieved. Droplet microfluidics for crystal size engineering and rapid micromixing can be utilized to advance time-resolved serial crystallography.

Light–oxygen–voltage (LOV) domains are small photosensory flavoprotein modules that allow the conversion of external stimuli (sunlight) into intra­cellular signals responsible for various cell behaviors (e.g. phototropism and chloro­plast relocation). This ability relies on the light-induced formation of a covalent thio­ether adduct between a flavin chromophore and a reactive cysteine from the protein environment, which triggers a cascade of structural changes that result in the activation of a serine/threonine (Ser/Thr) kinase. Recent developments in time-resolved crystallography may allow the activation cascade of the LOV domain to be observed in real time, which has been elusive. In this study, we report a robust protocol for the production and stable delivery of microcrystals of the LOV domain of phototropin Phot-1 from Chlamydomonas reinhardtii (CrPhotLOV1) with a high-viscosity injector for time-resolved serial synchrotron crystallography (TR-SSX). The detailed process covers all aspects, from sample optimization to data collection, which may serve as a guide for soluble protein preparation for TR-SSX. In addition, we show that the crystals obtained preserve the photoreactivity using infrared spectroscopy. Furthermore, the results of the TR-SSX experiment provide high-resolution insights into structural alterations of CrPhotLOV1 from Δt = 2.5 ms up to Δt = 95 ms post-photoactivation, including resolving the geometry of the thio­ether adduct and the C-terminal region implicated in the signal transduction process.

The advent of serial crystallography has rejuvenated and popularized room-temperature X-ray crystal structure determination. Structures determined at physiological temperature reveal protein flexibility and dynamics. In addition, challenging samples (e.g. large complexes, membrane proteins and viruses) form fragile crystals that are often difficult to harvest for cryo-crystallography. Moreover, a typical serial crystallography experiment requires a large number of microcrystals, mainly achievable through batch crystallization. Many medically relevant samples are expressed in mammalian cell lines, producing a meager quantity of protein that is incompatible with batch crystallization. This can limit the scope of serial crystallography approaches. Direct in situ data collection from a 96-well crystallization plate enables not only the identification of the best diffracting crystallization condition but also the possibility for structure determination under ambient conditions. Here, we describe an in situ serial crystallography (iSX) approach, facilitating direct measurement from crystallization plates mounted on a rapidly exchangeable universal plate holder deployed at a microfocus beamline, ID23-2, at the European Synchrotron Radiation Facility. We applied our iSX approach on a challenging project, autotaxin, a therapeutic target expressed in a stable human cell line, to determine the structure in the lowest-symmetry P1 space group at 3.0 Å resolution. Our in situ data collection strategy provided a complete dataset for structure determination while screening various crystallization conditions. Our data analysis reveals that the iSX approach is highly efficient at a microfocus beamline, improving throughput and demonstrating how crystallization plates can be routinely used as an alternative method of presenting samples for serial crystallography experiments at synchrotrons.

Structure-based drug design is highly dependent on the availability of structures of the protein of interest in complex with lead compounds. Ideally, this information can be used to guide the chemical optimization of a compound into a pharmaceutical drug candidate. A limitation of the main structural method used today – conventional X-ray crystallography – is that it only provides structural information about the protein complex in its frozen state. Serial crystallography is a relatively new approach that offers the possibility to study protein structures at room temperature (RT). Here, we explore the use of serial crystallography to determine the structures of the pharmaceutical target, soluble epoxide hydro­lase. We introduce a new method to screen for optimal microcrystallization conditions suitable for use in serial crystallography and present a number of RT ligand-bound structures of our target protein. From a comparison between the RT structural data and previously published cryo-temperature structures, we describe an example of a temperature-dependent difference in the ligand-binding mode and observe that flexible loops are better resolved at RT. Finally, we discuss the current limitations and potential future advances of serial crystallography for use within pharmaceutical drug discovery.

OaPAC is a recently discovered blue-light-using flavin adenosine dinucleotide (BLUF) photoactivated adenylate cyclase from the cyanobacterium Oscillatoria acuminata that uses adenosine triphosphate and translates the light signal into the production of cyclic adenosine monophosphate. Here, we report crystal structures of the enzyme in the absence of its natural substrate determined from room-temperature serial crystallography data collected at both an X-ray free-electron laser and a synchrotron, and we compare these structures with cryo-macromolecular crystallography structures obtained at a synchrotron by us and others. These results reveal slight differences in the structure of the enzyme due to data collection at different temperatures and X-ray sources. We further investigate the effect of the Y6W mutation in the BLUF domain, a mutation which results in a rearrangement of the hydrogen-bond network around the flavin and a notable rotation of the side chain of the critical Gln48 residue. These studies pave the way for picosecond–millisecond time-resolved serial crystallography experiments at X-ray free-electron lasers and synchrotrons in order to determine the early structural intermediates and correlate them with the well studied pico­second–millisecond spectroscopic intermediates.

Serial crystallography experiments at synchrotron and X-ray free-electron laser (XFEL) sources are producing crystallographic data sets of ever-increasing volume. While these experiments have large data sets and high-frame-rate detectors (around 3520 frames per second), only a small percentage of the data are useful for downstream analysis. Thus, an efficient and real-time data classification pipeline is essential to differentiate reliably between useful and non-useful images, typically known as `hit' and `miss', respectively, and keep only hit images on disk for further analysis such as peak finding and indexing. While feature-point extraction is a key component of modern approaches to image classification, existing approaches require computationally expensive patch preprocessing to handle perspective distortion. This paper proposes a pipeline to categorize the data, consisting of a real-time feature extraction algorithm called modified and parallelized FAST (MP-FAST), an image descriptor and a machine learning classifier. For parallelizing the primary operations of the proposed pipeline, central processing units, graphics processing units and field-programmable gate arrays are implemented and their performances compared. Finally, MP-FAST-based image classification is evaluated using a multi-layer perceptron on various data sets, including both synthetic and experimental data. This approach demonstrates superior performance compared with other feature extractors and classifiers.

Serial crystallography (SX) involves combining observations from a very large number of diffraction patterns coming from crystals in random orientations. To compile a complete data set, these patterns must be indexed (i.e. their orientation determined), integrated and merged. Introduced here is TORO (Torch-powered robust optimization) Indexer, a robust and adaptable indexing algorithm developed using the PyTorch framework. TORO is capable of operating on graphics processing units (GPUs), central processing units (CPUs) and other hardware accelerators supported by PyTorch, ensuring compatibility with a wide variety of computational setups. In tests, TORO outpaces existing solutions, indexing thousands of frames per second when running on GPUs, which positions it as an attractive candidate to produce real-time indexing and user feedback. The algorithm streamlines some of the ideas introduced by previous indexers like DIALS real-space grid search [Gildea, Waterman, Parkhurst, Axford, Sutton, Stuart, Sauter, Evans & Winter (2014). Acta Cryst. D70, 2652–2666] and XGandalf [Gevorkov, Yefanov, Barty, White, Mariani, Brehm, Tolstikova, Grigat & Chapman (2019). Acta Cryst. A75, 694–704] and refines them using faster and principled robust optimization techniques which result in a concise code base consisting of less than 500 lines. On the basis of evaluations across four proteins, TORO consistently matches, and in certain instances outperforms, established algorithms such as XGandalf and MOSFLM [Powell (1999). Acta Cryst. D55, 1690–1695], occasionally amplifying the quality of the consolidated data while achieving superior indexing speed. The inherent modularity of TORO and the versatility of PyTorch code bases facilitate its deployment into a wide array of architectures, software platforms and bespoke applications, highlighting its prospective significance in SX.

Compared with batch and vapor diffusion methods, counter diffusion can generate larger and higher-quality protein crystals yielding improved diffraction data and higher-resolution structures. Typically, counter-diffusion experiments are conducted in elongated chambers, such as glass capillaries, and the crystals are either directly measured in the capillary or extracted and mounted at the X-ray beamline. Despite the advantages of counter-diffusion protein crystallization, there are few fixed-target devices that utilize counter diffusion for crystallization. In this article, different designs of user-friendly counter-diffusion chambers are presented which can be used to grow large protein crystals in a 2D polymer microfluidic fixed-target chip. Methods for rapid chip fabrication using commercially available thin-film materials such as Mylar, propyl­ene and Kapton are also detailed. Rules of thumb are provided to tune the nucleation and crystal growth to meet users' needs while minimizing sample consumption. These designs provide a reliable approach to forming large crystals and maintaining their hydration for weeks and even months. This allows ample time to grow, select and preserve the best crystal batches before X-ray beam time. Importantly, the fixed-target microfluidic chip has a low background scatter and can be directly used at beamlines without any crystal handling, enabling crystal quality to be preserved. The approach is demonstrated with serial diffraction of photoactive yellow protein, yielding 1.32 Å resolution at room temperature. Fabrication of this standard microfluidic chip with commercially available thin films greatly simplifies fabrication and provides enhanced stability under vacuum. These advances will further broaden microfluidic fixed-target utilization by crystallographers.

Picture the Pacific Ocean of the 16th century. Spanish Galleons sail the wide open seas, carrying precious cargo like silver, porcelain, and textiles. The waters are dangerous; ship logs show concerns over pirates. But pirates are not to blame for a mysterious event that keeps happening.

For, you see, one in five of the ships leaving from the port of Manila didn't make it to Acapulco. It's a shipwrecking rate much higher than rates for other routes of the time. And the mystery of the serial shipwrecking Spanish ships remains unsolved, until today.

Everyone involved with these Spanish ships were aligned in a goal: Don't wreck the Spanish ships. And yet, wreck they did. Three economists took a look at the incentives for profit and risk at the time, and found the key to unlocking this ancient booty (of knowledge).

Our show today was produced by James Sneed, edited by Jess Jiang, fact-checked by Sierra Juarez, and engineered by Cena Loffredo. Alex Goldmark is Planet Money's executive producer.

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METTLER TOLEDO's PCE T2620 is a compact system for the track & trace labeling­ of cartons in accordance with the Falsified Medicines Directive in Europe, the Drug Supply Chain Security Act in the U.S., and other global regulations. 

Covectra designed the AuthentiTrack solution to ensure product integrity. It is intended to combat counterfeiting and diversion across a wide range of industries including food & beverage, electronics, fashion, cosmetics, luxury goods and more.

Antares Vision Group has implemented a traceability solution for cosmeceutical skin care provider Episciences, Inc., addressing supply chain visibility and gray market diversion challenges. The system was developed by ACSIS, a business unit of Antares Vision Group.

The packaging industry supports pharmaceutical and health sectors, with serialization playing a transformative role in ensuring compliance with regulations.

Serial asynchronous automatic dialling and control

Serial asynchronous automatic dialling and control

Asynchronous serial command interface for assistive and multi-functional communication devices

Oregon OSHA fined JAM Construction $103,438 for failing to protect workers from fall hazards, marking the company's third violation since April 2022.  

A silent crisis of ‘serial returns’ is eroding retail profit margins as uncovered in the Annual Returns Benchmark Report 2024* conducted by returns specialist ZigZag, in partnership with Retail Economics, accounting for 1 in 10 (11% of) online shoppers that make returns, are generating a quarter (24%) of all online non-food returns.

Domino Printing Sciences (Domino) has launched the new U510 – a state-of-the-art UV laser coder for permanent codes on white and coloured plastics, including high- and low-density polyethylene (HDPE and LDPE) pharmaceutical bottles. 

He sat motionless as the judge gave him his sentence.

Prosecutors have convicted Kwesi Hudson on 15 charges, including multiple felonies. Hudson, 50, was indicted in 2018 for a string of robberies, kidnappings, and sexual assault against three victims in February and March of 2017. “These heinous crimes victimized three women and terrorized an entire community for months,” said Attorney General Kathy Jennings. “I’m grateful […]

A Kent County couple has been indicted on 646 charges, including more than 80 felonies, for abusing their two children. Over a period of 20 months, Mary Vinson, 45, and Charles Vinson, 36, are alleged to have abused their children, including making them stand for long periods of time; withholding food; force feeding them; and […]

A New Castle man has been sentenced to life in prison following convictions in two domestic violence cases. On September 22, David Jewell, 47, was declared a habitual offender and sentenced to life in prison for convictions of felony Stalking, Harassment, and 24 counts of Terroristic Threatening, following an investigation into hundreds of violent phone calls and messages targeting two victims, one of whom was a […]

The Delaware Department of Justice has secured multiple felony convictions against a Kent County couple charged with the serial abuse and torture of their children, Attorney General Kathy Jennings announced Wednesday. “These are the cases that keep us up at night,” said Attorney General Jennings. “The pain that these children endured — and that it […]

A Kent County couple will effectively spend their lives in prison for the serial abuse and torture of their children, Attorney General Kathy Jennings announced today. “These children went through hell,” said Attorney General Jennings. “Now they never have to worry about their abusers again. Even when these cases are strong, they are not easy. […]

Autolus Therapeutics’ Aucatzyl is now FDA approved for treating advanced cases of B-cell precursor acute lymphoblastic leukemia. While it goes after the same target as Gilead Sciences’ Tecartus, Autolus engineered its CAR T-therapy with properties that could improve safety, efficacy, and durability.

The post ‘Serial Killing’ Cell Therapy From Autolus Lands FDA Approval in Blood Cancer appeared first on MedCity News.

Inflammation in men may be strongly related to years lived alone and/or serial break-ups, thereby indicating the heightened risk of ill health and death

TV Serial : অহনার চরিত্র থেকে সরে এলেন অভিনেত্রী! এই চরিত্রে রোশনিকে না দেখাটা দর্শকদের কাছে হতাশাজনক।  ধারাবাহিক থেকে হঠাৎ সরে আসার কারণ হিসেবে রোশনি জানিয়েছেন...

ಜೀ ಕನ್ನಡದಲ್ಲಿ ಬರುತ್ತಿರುವ 'ಅಣ್ಣಯ್ಯ' ಧಾರಾವಾಹಿ ಈಗಂತೂ ಎಲ್ಲರ ಮೆಚ್ಚಿನ ಧಾರಾವಾಹಿಯಾಗಿದೆ. ಪಾರು-ಶಿವಣ್ಣ ಒಂದಾಗಬೇಕು ಎಂದೇ ತಂಗಿಯಂದಿರು ಬಯಸುತ್ತಿದ್ದರು. ಶಿವು ಕೂಡ ಪಾರುಳನ್ನು ಬೆಟ್ಟಕ್ಕಿಂತ ಹೆಚ್ಚು ಪ್ರೀತಿಸುತ್ತಿದ್ದ. ಆದರೆ ಪಾರು ಆಸೆ, ಪ್ರೀತಿ ಬೇರೆಯೇ ಆಗಿತ್ತು. ಶಿವು ಬಿಟ್ಟು ಸಿದ್ಧಾರ್ಥ್ ನ ಲವ್ ಮಾಡ್ತಾ ಇದ್ಲು. ಇದು ಪಾರು ಆಸೆಯಾದರೆ ವಿಧಿಯ ಆಸೆ ಬೇರೆಯೇ ಆಗಿತ್ತು. ಶಿವು

Houndmills, Basingstoke ; New York, NY : Palgrave Macmillan, 2014.