https://www.cpa.com/system/files/cpa/infographics/top-challenges-facing-firms-performing-pcr-services-onpoint-cpacom_0.pdf

[Africa CDC] Addis Ababa, Ethiopia -- Africa Centres for Disease Control and Prevention through its Diagnostic Advisory Committee (DAC) has recommended the first locally manufactured Real-Time PCR test for mpox from Morocco. Africa CDC's approval underscores the test's reliability and efficacy, potentially boosting Morocco's role in global health initiatives.

APCR is pleased to announce the acquittal of Nazmuddin alias Bhola and Mohd Ajeej, who were arrested in February 2020 in connection with the North-East Delhi riots. They faced charges under several sections of the Indian Penal Code (IPC)

Thermo Fisher Scientific has simplified the workflow for the SureTect PCR System, by introducing streamlined handling and fail-safe color-coded plates and reagents, to smart, intuitive software and instrumentation.

Amcor unveiled its latest innovation: a 1-liter PET bottle for carbonated soft drink (CSD) use that is made from 100% post-consumer recycled (PCR) content.

The Pennsylvania Compensation Rating Bureau announced that eligible carriers can request refunds of 2009 work comp security fund assessments through Feb. 24. Gov. Josh Shapiro in July signed HB 2310, a…

In this report, we highlight that direct PCR, an extraction-free workflow: Can serve as an alternative to an extraction-based workflow for simpler, streaml

The 250ml Domino bottle includes a 75-mm-wide front face and customizable side panels.

The update emphasizes collaboration among cross-functional stakeholders—such as procurement, R&D, and corporate sustainability—who often have competing priorities like sustainability commitments and cost control. 

Guittard Couverture showcases the brand's commitment to providing customers with premium chocolate in enhanced, sustainable packaging.

After a tantalizing but all too brief teaser, a full trailer has been released for ITV's upcoming miniseries version of Len Deighton's famous first spy novel, THE IPCRESS FILE. THE IPCRESS FILE was first filmed in 1965 starring Michael Caine, and the film is an absolute classic. But as Deighton readers know, it necessarily omitted much of the novel. While director Sidney Fury wisely focused on the London portions of the book, it's clear from this trailer that the miniseries will include Harry Palmer's memorable sojourns to Beirut and a Pacific atoll, as well as a snowy landscape that was filmed in Finland, a location not found in the book, but featured in Deighton's later novel about the same protagonist (unnamed on the page) BILLION DOLLAR BRAIN. Perhaps the miniseries will already lay the groundwork for things to come. I'm already hoping it's a smash hit and gets multiple seasons (largely because I desperately want to see the second novel in the series, HORSE UNDER WATER, adapted for the screen; producer Harry Saltzman skipped it in the Sixties). But I'm getting ahead of myself. For now, we've still got THE IPCRESS FILE to look forward to! And based on this trailer, I can hardly wait! (For the moment I can't embed it due to privacy settings, but you can follow the link to watch it on Vimeo.)

Location: Electronic Resource- 

Modern, intuitive, and reliable thermal cyclers excel at optimizing sequencing, cloning, and genotyping throughput.

G protein–coupled receptors (GPCRs) initiate signaling cascades via G-proteins and beta-arrestins (βarr). βarr-dependent actions begin with recruitment of βarr to the phosphorylated receptor tail and are followed by engagement with the receptor core. βarrs are known to act as adaptor proteins binding receptors and various effectors, but it is unclear whether in addition to the scaffolding role βarrs can allosterically activate their downstream targets. Here we demonstrate the direct allosteric activation of proto-oncogene kinase Src by GPCR–βarr complexes in vitro and establish the conformational basis of the activation. Whereas free βarr1 had no effect on Src activity, βarr1 in complex with M2 muscarinic or β2-adrenergic receptors reconstituted in lipid nanodiscs activate Src by reducing the lag phase in Src autophosphorylation. Interestingly, receptor–βarr1 complexes formed with a βarr1 mutant, in which the finger-loop, required to interact with the receptor core, has been deleted, fully retain the ability to activate Src. Similarly, βarr1 in complex with only a phosphorylated C-terminal tail of the vasopressin 2 receptor activates Src as efficiently as GPCR–βarr complexes. In contrast, βarr1 and chimeric M2 receptor with nonphosphorylated C-terminal tail failed to activate Src. Taken together, these data demonstrate that the phosphorylated GPCR tail interaction with βarr1 is necessary and sufficient to empower it to allosterically activate Src. Our findings may have implications for understanding more broadly the mechanisms of allosteric activation of downstream targets by βarrs.

Rhodopsin is a canonical class A photosensitive G protein–coupled receptor (GPCR), yet relatively few pharmaceutical agents targeting this visual receptor have been identified, in part due to the unique characteristics of its light-sensitive, covalently bound retinal ligands. Rhodopsin becomes activated when light isomerizes 11-cis-retinal into an agonist, all-trans-retinal (ATR), which enables the receptor to activate its G protein. We have previously demonstrated that, despite being covalently bound, ATR can display properties of equilibrium binding, yet how this is accomplished is unknown. Here, we describe a new approach for both identifying compounds that can activate and attenuate rhodopsin and testing the hypothesis that opsin binds retinal in equilibrium. Our method uses opsin-based fluorescent sensors, which directly report the formation of active receptor conformations by detecting the binding of G protein or arrestin fragments that have been fused onto the receptor's C terminus. We show that these biosensors can be used to monitor equilibrium binding of the agonist, ATR, as well as the noncovalent binding of β-ionone, an antagonist for G protein activation. Finally, we use these novel biosensors to observe ATR release from an activated, unlabeled receptor and its subsequent transfer to the sensor in real time. Taken together, these data support the retinal equilibrium binding hypothesis. The approach we describe should prove directly translatable to other GPCRs, providing a new tool for ligand discovery and mutant characterization.

1. Ms. Shumayla Zafri and Ms. Lubhna Jahan, learned counsel for the petitioner.

2. Mr. Kuldeep Singh Rawal, learned AGA assisted by Mrs. Meenakshi Sharma, learned Brief Holder for the State/respondents no.1.

3. Heard.

4. By means of this writ petition, the petitioner has challenged the First Information Report No.0587 of 2023 dated 15.12.2023 for the offence punishable under Sections 420, 467, 468 & 471 of IPC registered with Police Station Kotwali, District Dehradun.

Gets the password out of encrypted ZIP files

The Union Health Ministry said on Saturday the COVID-19 patients who were severely ill will have to test negative through RT-PCR test before being discharged from a hospital. This decision is part of the revised discharge policy issued by the government. The ministry said, "The revised discharge policy is aligned with the guidelines on the 3-tier COVID facilities and the categorisation of the patients based on clinical severity." Patients having mild, very mild and pre-symptomatic and also moderate cases of COVID-19 do not require the RT-PCR test before discharge.

'Punjab paying price'
Punjab Chief Minister Captain Amarinder Singh lashed out at the Maharashtra government, alleging that it had lied when stating that migrants working in Nanded had undergone a COVID-19 test. On reaching Punjab, 969 of them tested positive, which Singh blames on the Maha Agadhi-led Maharashtra government in which the Congress is an ally of the Shiv Sena.

When mentioned that initially Punjab contained the virus well but of late, there has been a spurt in the COVID-19 tally, he said, "Yes, there has been a spurt in the cases because of the large number of migrants who came back from Nanded and Rajasthan. Suddenly, we saw around 7,000 people entering Punjab from these states on a single day."

The CM continued, "Even though we were assured by the Maharashtra government that all the pilgrims being sent back from Nanded had been tested thrice, it turned out that they had only been screened and no testing was done. We are paying the price for their negligence."

13 CISF men test positive
In a big scare for the Central Industrial Security Force (CISF), at least 13 more personnel of the force have tested positive, out of which 10 were deployed with the Delhi Metro Rail Corporation (DMRC). Till date, 543 Central Armed Police Force (CAPF) troops have tested positive across the country.

JNU to return to classes
With restrictions easing out and shops opening, the Jawaharlal Nehru University (JNU) too is all set to restart. The students are expected to return to their classrooms between June 25 and June 30.

The new academic calendar was announced keeping in view of the pandemic and the UGC guidelines. "This academic calendar has been unanimously approved by all the Deans of Schools and Chairpersons of Special Centres," read a statement issued by JNU vice Chancellor Jagadesh Kumar.

Chat portal to help migrants
To help the migrant workers stranded in several states, the Congress, on Saturday, launched a web portal in UP, even as the political slugfest continued over rail fares of migrants being ferried by Shramik Express trains. The Congress launched the portal to help UP workers stranded in other states as well as those stuck in the state. The portal has been developed by Valuefirst free of cost.

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RT-PCR test not must to discharge mild cases

Developer and SEO/marketing teams can proactively test web pages for SEO impact

This disclosure relates to combination therapies comprising anti-Pseudomonas Psl and PcrV bispecific binding molecules and related compositions, for use in prevention and treatment of Pseudomonas infection.

The Government is offering free PCR and antibody testing to the general public beginning Saturday, May 9th between 2pm  – 7pm at the Southside...

There is evidence that Kingella kingae, the major bacterial cause of osteoarticular infection in children <4 years of age, first colonizes the oropharynx before penetrating the bloodstream and invading distant organs. Diagnosis remains challenging because clinical findings at admission may be normal.

Our study demonstrated for the first time that a simple technique of detecting of K kingae DNA in the oropharynx can provide strong evidence that this microorganism is responsible for the OAI, or even stronger evidence that it is not. (Read the full article)

Quantitative real-time polymerase chain reaction allows sensitive detection of respiratory viruses. The clinical significance of detection of specific viruses is not fully understood, however, and several viruses have been detected in the respiratory tract of asymptomatic children.

Our results indicate that quantitative real-time polymerase chain reaction is limited at distinguishing acute infection from detection in asymptomatic children for rhinovirus, bocavirus, adenovirus, enterovirus, and coronavirus. (Read the full article)

Herpes encephalitis outside the neonatal period is typically severe and recognizable to clinicians. Excessive testing for herpes encephalitis is associated with increased medical costs and hospital length of stay, and risks patient harm.

Herpes testing and empirical acyclovir treatment in older and less unwell patients has been increasing in US pediatric hospitals over the past decade, which may reflect a more fundamental problem in current approaches to clinical decision-making. (Read the full article)

In a sign of the times and things to come, in-person cardiovascular society meetings pivot to virtual events.

Routine identification of fungal pathogens from positive blood cultures by culture-based methods can be time-consuming, delaying treatment with appropriate antifungal agents. The GenMark Dx ePlex investigational use only blood culture identification fungal pathogen panel (BCID-FP) rapidly detects 15 fungal targets simultaneously in blood culture samples positive for fungi by Gram staining. We aimed to determine the performance of the BCID-FP in a multicenter clinical study. Blood culture samples collected at 10 United States sites and tested with BCID-FP at 4 sites were compared to the standard-of-care microbiological and biochemical techniques, fluorescence in situ hybridization using peptide nucleic acid probes (PNA-FISH) and matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS). Discrepant results were analyzed by bi-directional PCR/sequencing of residual blood culture samples. A total of 866 clinical samples, 120 retrospectively and 21 prospectively collected, along with 725 contrived samples were evaluated. Sensitivity and specificity of detection of Candida species (C. albicans, C. auris, C. dubliniensis, C. famata, C. glabrata, C. guilliermondii, C. kefyr, C. krusei, C. lusitaniae, C. parapsilosis, and C. tropicalis) ranged from 97.1 to 100% and 99.8 to 100%, respectively. For the other organism targets, sensitivity and specificity were as follows: 100% each for Cryptococcus neoformans and C. gattii, 98.6% and 100% for Fusarium spp., and 96.2% and 99.9% for Rhodotorula spp., respectively. In 4 of the 141 clinical samples, the BCID-FP panel correctly identified an additional Candida species, undetected by standard-of-care methods. The BCID-FP panel offers a faster turnaround time for identification of fungal pathogens in positive blood cultures that may allow for earlier antifungal interventions and includes C. auris, a highly multidrug-resistant fungus.

Infections due to methicillin-resistant Staphylococcus aureus (MRSA) are present worldwide and represent a major public health concern. The capability of PCR followed by high-resolution melt (HRM) curve analysis for the detection of community-associated and livestock-associated MRSA strains and the identification of staphylococcal protein A (spa) locus was evaluated in 74 MRSA samples which were isolated from the environment, humans, and pigs on a single piggery. PCR-HRM curve analysis identified four spa types among MRSA samples and differentiated MRSA strains accordingly. A nonsubjective differentiation model was developed according to genetic confidence percentage values produced by tested samples, which did not require visual interpretation of HRM curve results. The test was carried out at different settings, and result data were reanalyzed and confirmed with DNA sequencing. PCR-HRM curve analysis proved to be a robust and reliable test for spa typing and can be used as a tool in epidemiological studies.

Quantitative bacterial culture of bronchoalveolar lavage fluids (BALF) is labor-intensive, and the delay involved in performing culture, definitive identification, and susceptibility testing often results in prolonged use of broad-spectrum antibiotics. The Unyvero lower respiratory tract (LRT) panel (Curetis, Holzgerlingen, Germany) allows the multiplexed rapid detection and identification of 20 potential etiologic agents of pneumonia within 5 h of collection. In addition, the assay includes detection of gene sequences that confer antimicrobial resistance. We retrospectively compared the performance of the molecular panel to routine quantitative bacterial culture methods on remnant BALF. Upon testing 175 BALF, we were able to analyze positive agreement of 181 targets from 129 samples, and 46 samples were negative. The positive percent agreement (PPA) among the microbial targets was 96.5%, and the negative percent agreement (NPA) was 99.6%. The targets with a PPA of <100% were Staphylococcus aureus (34/37 [91.9%]), Streptococcus pneumoniae (10/11 [90.9%]), and Enterobacter cloacae complex (2/4 [50%]). For the analyzable resistance targets, concordance with phenotypic susceptibility testing was 79% (14/18). This study found the Unyvero LRT panel largely concordant with culture results; however, no outcome or clinical impact studies were performed.

On 31 December 2019, the World Health Organization was informed of a cluster of cases of pneumonia of unknown etiology in Wuhan, China. Subsequent investigations identified a novel coronavirus, now named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), from the affected patients. Highly sensitive and specific laboratory diagnostics are important for controlling the rapidly evolving SARS-CoV-2-associated coronavirus disease 2019 (COVID-19) epidemic. In this study, we developed and compared the performance of three novel real-time reverse transcription-PCR (RT-PCR) assays targeting the RNA-dependent RNA polymerase (RdRp)/helicase (Hel), spike (S), and nucleocapsid (N) genes of SARS-CoV-2 with that of the reported RdRp-P2 assay, which is used in >30 European laboratories. Among the three novel assays, the COVID-19-RdRp/Hel assay had the lowest limit of detection in vitro (1.8 50% tissue culture infective doses [TCID₅₀]/ml with genomic RNA and 11.2 RNA copies/reaction with in vitro RNA transcripts). Among 273 specimens from 15 patients with laboratory-confirmed COVID-19 in Hong Kong, 77 (28.2%) were positive by both the COVID-19-RdRp/Hel and RdRp-P2 assays. The COVID-19-RdRp/Hel assay was positive for an additional 42 RdRp-P2-negative specimens (119/273 [43.6%] versus 77/273 [28.2%]; P < 0.001), including 29/120 (24.2%) respiratory tract specimens and 13/153 (8.5%) non-respiratory tract specimens. The mean viral load of these specimens was 3.21 x 10⁴ RNA copies/ml (range, 2.21 x 10² to 4.71 x 10⁵ RNA copies/ml). The COVID-19-RdRp/Hel assay did not cross-react with other human-pathogenic coronaviruses and respiratory pathogens in cell culture and clinical specimens, whereas the RdRp-P2 assay cross-reacted with SARS-CoV in cell culture. The highly sensitive and specific COVID-19-RdRp/Hel assay may help to improve the laboratory diagnosis of COVID-19.

Adeno-associated virus (AAV) recombinants are currently the vector of choice for many gene therapy applications. As experimental therapies progress to clinical trials, the need to characterize recombinant adeno-associated viruses (rAAVs) accurately and reproducibly increases. Accurate determination of rAAV infectious titer is important for determining the activity of each vector lot and for ensuring lot-to-lot consistency. The following protocol developed in our laboratory uses a 96-well TCID₅₀ format and quantitative polymerase chain reaction (qPCR) detection for the determination of rAAV infectious titer.

This protocol is used to determine the concentration of DNase-resistant vector genomes (i.e., packaged in the capsid) in purified recombinant adeno-associated virus (rAAV) preparations. The protocol begins with treatment of the vector stock with DNase I to eliminate unencapsidated AAV DNA or contaminating plasmid DNA. This is followed by a heat treatment to heat-inactivate DNase I, to disrupt the viral capsid, and to release the packaged vector genomes for quantification by real-time polymerase chain reaction (PCR) using a set of standards (linearized plasmid used for vector production) containing known copy numbers. To accomplish high-throughput titration, the primer and probe sets used in real-time PCR are usually designed to target common elements present in most rAAV genomes, such as promoters and poly(A) signals. This strategy significantly reduces the number of PCRs, controls, and turnaround time. Several important controls should be included in the assay as follows: The first two controls should have a known copy number of the rAAV genome plasmid treated or not treated with DNase I. This control tests the effectiveness of DNase treatment. To control for potential cross-contamination between samples during the preparation process, a blank control containing nuclease-free water only should be processed and tested in parallel. A validation vector sample with a known titer should be included in every assay to monitor interassay variability. Finally, for the PCR run, a no-template control (NTC) is included to indicate cross-contamination during PCR setup.

Hemp isn't just for food, textile fiber, and fuel, but can also be a renewable and sustainable component of green buildings, as this crowdfunded project attempts to show.

New Delhi, May 3 (ICMR) Million RT-PCR tests for novel coronavirus have been conducted so far in India. In a press statement the apex medical research

A new helpline has been launched by the Delhi Commission for Protection of Child Rights (DCPCR) to provide counseling support to children and parents

Diagnostics subsidiary Aster Clinical Lab LLP has set up its pathology reference laboratory at Bengaluru.

This population epidemiology study characterizes trends in polymerase chain reaction (PCR) test positivity for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Washington State and the Seattle area between March 1 and April 16, 2020, before and after statewide physical distancing guidelines and stay-at-home orders.

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