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Spatially isolated reactions in a complex array: using magnetic beads to purify and quantify nucleic acids with digital and quantitative real-time PCR in thousands of parallel microwells

Lab Chip, 2020, Advance Article
DOI: 10.1039/D0LC00069H, Paper
W. Hampton Henley, Nathan A. Siegfried, J. Michael Ramsey
Encoded beads carrying primer pairs for nucleic acid targets are used for sample preparation and multiplexed-in-space digital PCR quantification.
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pcr

Molecular analysis of edible bird’s nest and rapid authentication of Aerodramus fuciphagus from its subspecies by PCR-RFLP based on the cytb gene

Anal. Methods, 2020, Accepted Manuscript
DOI: 10.1039/C9AY02548K, Paper
Kunfeng Liu, Maoyong Wu, Xuemei Lin, Piyanuch Lonan, Sitai Chen, Yina Wu, Xiaoping Lai, Liangwen Yu, Xiaoming Zhou, Geng Li
Edible bird's nest (EBN), for its great nutritional value, is widely used around the world, especially in China and Singapore. EBNs of different origins and types may vary in price...
The content of this RSS Feed (c) The Royal Society of Chemistry




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[ASAP] “Sample-to-Answer” Detection of Rare ctDNA Mutation from 2 mL Plasma with a Fully Integrated DNA Extraction and Digital Droplet PCR Microdevice for Liquid Biopsy

Analytical Chemistry
DOI: 10.1021/acs.analchem.0c00818




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T.N. to order 10 lakh more RT-PCR testing kits

Tamil Nadu is ordering 10 lakh more RT-PCR (reverse transcription-polymerase chain reaction) testing kits to ramp up testing across the State.Accordin




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Differential coupling of RGS3s and RGS4 to GPCR-GIRK channel signaling complexes




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Use of BOX-PCR subtyping of Escherichia coli and Enterococcus spp. to determine the source of microbial contamination at a Florida beach




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Evaluation of TaqMan real-time PCR for the detection of viable Cryptosporidium parvum oocysts in environmental water samples




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Determination of decay rates and differential survival of Escherichia coli and Enterococcus spp. under hydrodynamically active conditions using BOX-PCR typing




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The development of a human polyomavirus quantitative pcr assay to assess viral persistence, water quality, and human health risks




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Longitudinal surveillance of SARS-like coronaviruses in bats by quantitative real-time PCR




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Mylab to manufacture up to 2 lakh COVID-19 RT-PCR test per day




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CFSL-36 is ready to assist UT admin with its RT-PCR technique