David Guerit, Pauline Marie, Anne Morel, Justine Maurin, Christel Verollet, Brigitte Raynaud-Messina, Serge Urbach, and Anne Blangy

Among hematopoietic cells, osteoclasts (Oc) and immature dendritic cells (Dc) are closely related myeloid cells with distinct functions; Oc participate skeleton maintenance while Dc sample the environment for foreign antigens. Such specificities rely on profound modifications of gene and protein expression during Oc and Dc differentiation. We provide global proteomic and transcriptomic analyses of primary mouse Oc and Dc, based on original SILAC and RNAseq data. We established specific signatures for Oc and Dc including genes and proteins of unknown functions. In particular, we showed that Oc and Dc have the same α and β tubulin isotypes repertoire but that Oc express much more β tubulin isotype Tubb6. In both mouse and human Oc, we demonstrate that elevated expression of Tubb6 in Oc is necessary for correct podosomes organization and thus for the structure of the sealing zone, which sustains the bone resorption apparatus. Hence, lowering Tubb6 expression hindered Oc resorption activity. Overall, we highlight here potential new regulators of Oc and Dc biology and illustrate the functional importance of the tubulin isotype repertoire in the biology of differentiated cells.

Victor J. F. Kitano, Yoko Ohyama, Chiyomi Hayashida, Junta Ito, Mari Okayasu, Takuya Sato, Toru Ogasawara, Maki Tsujita, Akemi Kakino, Jun Shimada, Tatsuya Sawamura, and Yoshiyuki Hakeda

Osteoporosis is associated with vessel diseases attributed to hyperlipidemia, and bone resorption by multinucleated osteoclasts is related to lipid metabolism. In this study, we generated low-density lipoprotein receptor (LDLR)/lectin-like oxidized LDL receptor-1 (LOX-1) double knockout (dKO) mice. We found that, like LDLR single KO (sKO), LDLR/LOX-1 dKO impaired cell-cell fusion of osteoclast-like cells (OCLs). LDLR/LOX-1 dKO and LDLR sKO preosteoclasts exhibited decreased uptake of LDL. The cell surface cholesterol levels of both LDLR/LOX-1 dKO and LDLR sKO osteoclasts were lower than the levels of wild-type OCLs. Additionally, the amount of phosphatidylethanolamine (PE) on the cell surface was attenuated in LDLR/LOX-1 dKO and LDLR sKO pre-OCLs, while the PE distribution in wild-type OCLs was concentrated on the filopodia in contact with neighboring cells. Abrogation of the ATP binding cassette G1 (ABCG1) transporter, which transfers PE to the cell surface, caused decreased PE translocation to the cell surface and subsequent cell-cell fusion. The findings of this study indicate the involvement of a novel cascade (LDLR~ABCG1~PE translocation to cell surface~cell-cell fusion) in multinucleation of OCLs.

Great white sharks, killer whales, sea lions, even polar bears—the ocean is full of giant predators. But one of the ocean’s worst enemies is not […]

The post Beautiful plastic sculptures tell ugly story of human garbage in the ocean appeared first on Smithsonian Insider.

Odile Madden knows a lot about plastic. A materials scientist with the Smithsonian Museum Conservation Institute, she has spent the past eight years studying plastics […]

The post Microplastics in our environment: A conversation with Odile Madden, Smithsonian plastics scientist appeared first on Smithsonian Insider.

This work studies the elastic scattering behavior of electron vortices when propagating through amorphous samples. A formulation of the multislice approach in cylindrical coordinates is used to theoretically investigate the redistribution of intensity between different angular momentum components due to scattering. To corroborate and elaborate on our theoretical results, extensive numerical simulations are performed on three model systems (Si3N4, Fe0.8B0.2, Pt) for a wide variety of experimental parameters to quantify the purity of the vortices, the net angular momentum transfer, and the variability of the results with respect to the random relative position between the electron beam and the scattering atoms. These results will help scientists to further improve the creation of electron vortices and enhance applications involving them.

The use of strongly bent crystals in spectrometers for pulses of a hard X-ray free-electron laser is explored theoretically. Diffraction is calculated in both dynamical and kinematical theories. It is shown that diffraction can be treated kinematically when the bending radius is small compared with the critical radius given by the ratio of the Bragg-case extinction length for the actual reflection to the Darwin width of this reflection. As a result, the spectral resolution is limited by the crystal thickness, rather than the extinction length, and can become better than the resolution of a planar dynamically diffracting crystal. As an example, it is demonstrated that spectra of the 12 keV pulses can be resolved in the 440 reflection from a 20 µm-thick diamond crystal bent to a radius of 10 cm.

Entangled embedded periodic nets and crystal frameworks are defined, along with their dimension type, homogeneity type, adjacency depth and periodic isotopy type. Periodic isotopy classifications are obtained for various families of embedded nets with small quotient graphs. The 25 periodic isotopy classes of depth-1 embedded nets with a single-vertex quotient graph are enumerated. Additionally, a classification is given of embeddings of n-fold copies of pcu with all connected components in a parallel orientation and n vertices in a repeat unit, as well as demonstrations of their maximal symmetry periodic isotopes. The methodology of linear graph knots on the flat 3-torus [0,1)3 is introduced. These graph knots, with linear edges, are spatial embeddings of the labelled quotient graphs of an embedded net which are associated with its periodicity bases.

George Smith, a marine biologist at the Smithsonian Environmental Research Center, explains his work of finding ways to prevent invasive species from being released in Baltimore Harbor in the ballast water of large ships.

The post Preventing ballast-water invasions of alien species appeared first on Smithsonian Insider.

It takes a Tlingit artist up to 2,000 hours, or 83 days, to weave just one ceremonial robe. Not surprisingly, this art form is practiced by a dedicated few including Tlingit artists Teri Rofkar and Shelly Laws of Alaska. In their presentation for the Smithsonian Spotlight series hosted by the Arctic Studies Center at the Anchorage Museum, Rofkar and Laws discuss the methods and cultural significance of robes, spruce root baskets and more.
For more information, go to http://www.mnh.si.edu/arctic/html/alaska.htm

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William Fitzhugh - Edward Nelson's 1877-1881 Western Alaskan Expedition

The post Smithsonian anthropologist William Fitzhugh speaks about Edward Nelson’s 1877-1881 Western Alaskan Expedition appeared first on Smithsonian Insider.

The post National Zoo gorillas participate in heart disease study appeared first on Smithsonian Insider.

The post 3-D laser scanning is helping solve ancient mysteries at the Smithsonian appeared first on Smithsonian Insider.

Elastic has created some of the most intriguing title sequences in recent television history. Directed by Patrick Clair (Australian b. 1982), the opening credits for […]

The post Elastic – Opening Credits: True Detective appeared first on Smithsonian Insider.

Join Dr. Elizabeth Cottrell as she explores the volcanoes of Alaska’s Western Aleutian Islands.

The post Smithsonian volcanologist Elizabeth Cottrell explores Alaskan Volcanoes appeared first on Smithsonian Insider.

Tuvan ensemble Alash demonstrates various traditional throat singing styles that they use in their music. Smithsonian Folkways Recordings video.

The post Alash demonstrates throat singing styles appeared first on Smithsonian Insider.

Aymar Ccopacatty (Aymara), NMAI Artist Leadership Program (ALP) participant, tells his personal story of the environmental impact on Puno, Peru, of all-too-commonly discarded commercial plastic […]

The post Knitting plastic Wiphalas in Peru appeared first on Smithsonian Insider.

Police say TSA agent Gerardo Hernandez was shot and killed last Friday at the base of the escalators of LAX Terminal 3, and not at the checkpoint gates.  Paul Ciancia is accused of killing Hernandez and wounding several others.  Ciancia remains hospitalized in critical condition.

Steve Julian: Business analyst Mark Lacter, how did the airlines respond to shooting and its aftermath?

Mark Lacter: Generally pretty well, Steve, considering that the airport was effectively closed for several hours on Friday, and most of Terminal 3 was out of commission until Saturday afternoon.  You know, there's always this precarious balance in operating airlines and airports, even in the best of circumstances.  Just so many flights coming in and going out, and so many thousands people using the facility at any given time - and it really doesn't take much to upset the balance.  So, when you have something horrific take place and you see all those travelers stranded outside the terminals, the ripple effects are enormous - not just at LAX but all over the country.

Julian: More than a thousand flights were either canceled or delayed on Friday.

Lacter: And, there was a further complication because the airlines flying out of Terminal 3 are not the legacy carriers like United, American, and Delta that have all kinds of resources, but smaller operations with less flexibility.  It's not like there's an empty aircraft just sitting in a hangar waiting to take passengers wherever they want to go.  Actually, the airlines have gotten better at arranging re-bookings when there's a snowstorm or some other emergency that gives them advance warning.  But obviously, there was no advance warning last Friday, so the carriers needed to improvise in handling passengers whose flights were cancelled.

Julian: What did they do?

Lacter: One step was waiving the fees normally charged to re-book flights (and that's gotten to be a pretty penny).  Another was waiving the difference in the price of the original ticket and the re-booked ticket.  But, the policies varied according to the airline, and we heard about travelers not receiving hotel or food vouchers, or having to buy a brand new ticket on another airline if they wanted to avoid the wait - and that can be expensive.  Which raises another issue: planes tend to be completely full these days because airlines have been cutting back on the number of flights.  And that can be a problem if you're taking a route that doesn't have too many flights in the first place.  So, it gets really complicated.

Julian: Why do you think we haven't we heard more horror stories from passengers?

Lacter: Well, look at the cities that the airlines in Terminal 3 fly to - New York, San Francisco, Seattle, Dallas.  They're all served by several other carriers.  L.A. to New York, in particular, is one of the busiest routes in the world, which means that it's also one of the most competitive.  So, even if your flight was cancelled, there's a good chance you'd be able to find space by Saturday (which is normally a slower day for air travel).  This is a big reason, in general, why people like LAX.

Julian: Why don't other local airports handle more of the load?

Lacter: You might remember a few years ago local officials were promoting something called "regionalization" - the idea was that as LAX maxed out on the number of passengers it was allowed to handle each year, then other airports would make up the difference - places like Ontario, Bob Hope in Burbank, and John Wayne in Orange County.

Julian: Right, and they talked about easing traffic congestion by spreading around the flights.

Lacter: Well, regionalization never happened because, first of all, passenger levels at L.A. International didn't come close to maxing out.  But, more importantly, because the airlines decided that using LAX was more efficient for everything from handling baggage to arranging international connections.  So, through the first nine months of the year, passenger traffic at LAX is up 4.2 percent from a year earlier, while at Ontario traffic was down 9.3 percent.  And, we've seen that John Wayne, Bob Hope, and Long Beach are all struggling.  Of course, the challenge at a busy place like LAX is making it as safe as possible, and that will no doubt become a priority in the weeks ahead.

Mark Lacter writes for Los Angeles Magazine and pens the business blog at LA Observed.com.

This content is from Southern California Public Radio. View the original story at SCPR.org.

Flash App+ is a Google Chrome extension that has a description of "This extension configures your Default Search to Flash App+ (searchpowerapp.com)".

This article was published first at Remove the Flash App+ Chrome Extension

The Smithsonian Environmental Research Center in Edgewater, Md., and the Alaska Sea Grant Program of the University of Alaska, Fairbanks, were recently identified as the […]

The post Smithsonian scientists to help identify and eradicate invasive species in Alaskan waters appeared first on Smithsonian Insider.

During the course of a year, a western gorilla in the Lossi Forest of Northern Congo stuffs its face with a profusion of fruit, including figs, […]

The post Gorillas Aren’t Tricked By a Faux Sugary Fruit Thanks to a Mutation appeared first on Smithsonian Insider.

Entangled embedded periodic nets and crystal frameworks are defined, along with their dimension type, homogeneity type, adjacency depth and periodic isotopy type.

Simon L. Bullock
Apr 6, 2020; 133:jcs245134-jcs245134
Meeting Report

Full Text:

Earth's magnetic field seems steady and true -- reliable enough to navigate by. Yet, largely hidden from daily life, the field drifts, waxes and wanes. The magnetic North Pole is currently shifting toward Siberia, forcing the Global Positioning System that underlies modern navigation to update its software sooner than expected. Every several hundred thousand years, the magnetic field dramatically shifts and reverses its polarity. Magnetic north flips to the geographic South Pole and, eventually, back again. This reversal has happened countless times over Earth's history, but scientists' understanding of why and how the field reverses is limited. The researchers find that the most recent field reversal 770,000 years ago took at least 22,000 years to complete, several times longer than previously thought. The results call into question controversial findings that some reversals could occur within a human lifetime.

Image credit: Brad Singer

The environmental effects of oil and gas exploration and production on Alaska s North Slope have been accumulating for more than three decades, says a new report from the National Academies National Research Council.

The National Academy of Sciences (NAS) and Institute of Medicine (IOM) today released SCIENCE, EVOLUTION, AND CREATIONISM, a book designed to give the public a comprehensive and up-to-date picture of the current scientific understanding of evolution and its importance in the science classroom.

A new data network that integrates emerging research on the molecular makeup of diseases with clinical data on individual patients could drive the development of a more accurate classification of disease and ultimately enhance diagnosis and treatment, says a new report from the National Research Council.

The U.S. electric power delivery system is vulnerable to terrorist attacks that could cause much more damage to the system than natural disasters such as Hurricane Sandy, blacking out large regions of the country for weeks or months and costing many billions of dollars, says a newly released report by the National Research Council.

Rates of physical and sexual abuse of children have declined over the last 20 years, but for reasons not fully understood, says a new report from the Institute of Medicine. Yet, reports of psychological and emotional child abuse have risen in the same period, and data vary significantly as to whether child neglect is increasing, decreasing, or remaining constant.

Since 1930, the FBI has served as central coordinator of data on known criminal offenses, combining reports from approximately 18,000 local law enforcement agencies under the Uniform Crime Reporting (UCR) Program.

The U.S. is losing ground in a second laser revolution of highly intense, ultrafast lasers that have broad applications in manufacturing, medicine, and national security, says a new report from the National Academies of Sciences, Engineering, and Medicine.

The emergence of inexpensive small unmanned aircraft systems (sUASs) that operate without a human pilot, commonly known as drones, has led to adversarial groups threatening deployed U.S. forces, especially infantry units.

Along with participation in the International Thermonuclear Experimental Reactor (ITER) project – a large, international burning plasma experiment – the U.S. Department of Energy (DOE) should start a national program of accompanying research and technology to build a compact pilot plant that produces electricity from fusion at the lowest possible capital cost, says a new report from the National Academies of Sciences, Engineering, and Medicine.

Current evidence supports the classification of the contemporary red wolf as a distinct species of wolf, although additional genomic evidence from historic wolf specimens could change that assessment, says Evaluating the Taxonomic Status of the Mexican Gray Wolf and the Red Wolf, a new report from the National Academies of Sciences, Engineering, and Medicine.

A new report from the National Academies of Sciences, Engineering, and Medicine offers guidance to the Consumer Product Safety Commission (CPSC) on how to conduct a hazard assessment of nonpolymeric, additive organohalogen flame retardants (OFRs), which are used in some consumer products.

Revelle Lecture Explores the Problem and Proposes Solutions

In light of the present situation in the U.S., we believe that it is essential to explore a wide range of options for treating the increasing numbers of very ill patients with COVID-19 respiratory illness.

From N-95 masks that are protecting health care workers during the COVID-19 pandemic to food packaging found in every aisle of the grocery store, plastics play an essential role in our lives.

Maurice Jackson of Proven and Probable discusses the latest news from Millrock Resources with the company's CEO.

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A 2018 exhibit at the Billy Ireland Cartoon Library & Museum at Ohio State University celebrated the artistic legacy of MAD magazine.; Credit: Andrew Welsh-Huggins/AP

The funny, freckled face of Alfred E. Neuman is more or less retiring.

One of the last widely circulated print satirical magazines in America will leave newsstands after this year, according to sources at DC Comics, which publishes MAD magazine.

While the Harvard Lampoon remains in business, The Onion hasn't been in print since 2013. The once-influential Spy was a casualty of the 1990s.

At MAD's peak in the early 1970s, more than 2 million people subscribed to it, both for its pungent political humor and deeply adolescent jokes.

In 2017, that number had reportedly dropped to 140,000.

MAD isn't completely shutting down, but it will be radically downsized and changed.

Readers will only be able to find the 67-year-old humor magazine at comic book stores and through subscriptions.

After issue No. 10 this fall, there will no longer be new content, except for end-of-year specials which will be all new. Starting with issue No. 11, the magazine will feature classic, best-of and nostalgic content, repackaged with new covers.

This content is from Southern California Public Radio. View the original story at SCPR.org.

Closing Night Party at last year's 2019 TCM 10th Annual Classic Film Festival in Hollywood, California. ; Credit: Presley Ann/Getty Images for TCM

Like all public events following the start of widespread stay-at-home orders from the state and federal government, the 2020 TCM Classic Film Festival was unfortunately cancelled this year due to health concerns posed by COVID-19. But festival faithful and classic film buffs won’t be left hanging this year.

Instead of a live, in person event, TCM decided to do a Special Home Edition of the annual festival that will air on the TCM Channel. The festival kicked off Thursday evening with a screening of the 1954 version of “A Star is Born” starring Judy Garland and James Mason and will include a number of films from past years’ festival lineups as well as ones that were slated for this year’s event. It ends late Sunday night (technically early Monday morning) with a screening of the 1982 film Victor/Victoria, for which Julie Andrews was slated to be in attendance at the 2020 festival before it was cancelled.

Today on FilmWeek, Turner Classic Movies hosts Ben Mankiewicz and Dave Karger join Larry Mantle to preview this year’s Special Home Edition of the TCM Classic Film Festival, talk about having to pivot due to the pandemic, and sharing some of their favorite films that are screening at this year’s event.

For a list of films and showtimes, click here.

Guests:

Ben Mankiewicz, host for Turner Classic Movies; he tweets @BenMank77

Dave Karger, host for Turner Classic Movies and special correspondent for the Internet Movie Database (IMDb); he tweets @DaveKarger

This content is from Southern California Public Radio. View the original story at SCPR.org.

Brad Bird accepts the Best Animated Feature Award for Incredibles 2 during The National Board of Review Annual Awards Gala at Cipriani 42nd Street on January 8, 2019 in New York City. ; Credit: Dia Dipasupil/Getty Images for National Board

Brad Bird has made a name for himself as the director of new age, animated classics, like “The Iron Giant” and “The Incredibles”. Now he’s teaming up with Turner Classic Movies to share his picks for classic movie watching that will keep you busy and entertained while you’re stuck at home.

Bird has curated a list of his favorite films which will air on this season’s “The Essentials” with TCM Primetime host Ben Mankiewicz. Every Saturday from May up to January of next year, TCM will air one of twenty movies chosen by Bird. His list includes big-name classics that any movie buff will recognize, like “Casablanca” and “Dr. Strangelove”. But Bird throws in a few surprises, one being that there are no animated films on his list. Another surprising thing viewers will notice is that about a third of the films included are musicals, like “Singin’ In The Rain” and “Guys And Dolls”. With TCM’s seemingly infinite library of movie classics to choose from, Bird says narrowing it down to only twenty favorites was a challenge. According to Bird, his list comprises movies that are not only entertaining but “transcend time” and “speak in a language that is still in many ways current”.

“The Essentials” was filmed in early December, but Bird’s list is coming out during a time when movie-going is looking very different these days. The virus has put the entire film industry on pause, with studios halting production and theaters closing their doors indefinitely. In lieu of new movie releases and regular theater-going, many are taking the opportunity to revisit the classics and Bird’s list provides a starting guide for which ones should be on your watch-list.

Today on FilmWeek, Brad Bird joins us to talk about his favorite movies, what projects he has lined up, and his thoughts on how the film industry is adjusting to and getting through the pandemic.

TCM’s “The Essentials” will air on Saturdays at 8 p.m. beginning May 2. For the full list and schedule of films, click here.

Guest:

Brad Bird, animator, director and screenwriter whose directing credits include Pixar’s “Ratatouille” and “The Incredibles” films and Disney’s “The Iron Giant”; he tweets at @BradBirdA113

This content is from Southern California Public Radio. View the original story at SCPR.org.

GlassWire is a free network monitoring tool that displays and alerts you about the network traffic originating from your computer.  This allows you to quickly see what applications are communicating over the network and the Internet, how much bandwidth they are using, and what hosts they are connecting to. GlassWire also maintains a database of suspicious sites and will alert you when you attempt to visit one of them. Last, but not least, GlassWire includes an easy to use application firewall that allows you to block specific applications from communicating over a network and the Internet. [...]

Data from studies monitoring the amount of plastic eaten by seabirds suggest that levels in the North Sea are well above targets established for the North East Atlantic Ocean by OSPAR (the Oslo and Paris Convention). For the most recent monitoring period, the target amount was exceeded in well over half the birds studied.

Maurice Jackson of Proven and Probable discusses the latest news from Millrock Resources with the company's CEO.