The Greenwood Redevelopment Commission has given city officials the green light to seek bids for a nearly 20-acre development project. Here's what it could look like.

One person died after the collapse of a well that was under construction in the 4900 block of W. Road 225 N. in Bargersville.

A Bargersville man died in a head-on crash in Johnson County early Saturday morning. Police believe alcohol was a factor in the crash.

The Pacers didn't have their starting point guard for the last three games before the NBA went on hiatus, but he's able to play if/when it restarts.

Officers were called to East 38th Street and N. Mitthoeffer Road shortly before 10 p.m. where they found a body lying in the roadway.

Watch highlights as Pascal Wehrlein takes a lights-to-flag win in the third Formula E Race At Home Challenge at Monaco which saw a "shunt that never ends" on the opening lap.

Indiana educators are among the strongest advocates for students and deserve the utmost appreciation and respect, Jennifer McCormick writes.

Shady Habash, whose video mocked the president, "mistook hand sanitiser for water", prosecutors say.

Before it recorded a single case of the virus, Kenya witnessed a number of anti-Chinese incidents.

Formula 1 boss Chase Carey says he plans to start the season in Austria in July, after France became the latest country to call off its race.

Formula 1 almost doubles the shutdown period for teams as the coronavirus crisis continues to play havoc with the 2020 championship.

An overview of the Pacific territory, including key facts, political leaders and notes on the media

New techniques are being used to transform images from Hubble into spectacular 3D visualisations.

The Blyth Festival will not be staging any plays this year.

Clashes erupt after people complain about a perceived failure to help the poor during the pandemic.

For the first time in 50 years there won't be a San Diego Comic Con.

Former defence minister says cargo plane shot down as it tried to land in the southern town of Bardale.

A Chicago cyclist was injured by an electric scooter rider who fled the scene. Now he's gone to court to get the city's scooter companies to turn over personal information about their riders and their history.

Sony has introduced a new product called the Reon Pocket, which operates like a mobile air conditioner that fits inside clothing.

A team of researchers at the University of California at San Diego have created a soft robotic lens that responds to eye movements.

• Tesla's vehicles are and always have been crammed with great ideas.
• These range from touchscreen interfaces to innovative battery designs to staggering acceleration.
• I've driven or experienced every vehicle Tesla has ever sold or intends to sell in the future.
• Here are all my favorite features, ranked.
• Visit Business Insider's homepage for more stories.

In about two decades, Tesla has done what everyone in the auto industry thought was impossible: create an all-electric brand that could sell hundreds of thousands of vehicles.

Tesla could have done that in a boring or modest way, developing  the equivalent of an electric VW Beetle.

Instead, Tesla made fantastically compelling cars that are fast, look amazing, and are packed with features.

Here's a rundown of all my favorites, ranked from bottom to top:

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Tesla has been in business for 17 years. In that period of time, it's consistently captivated the world not just because it makes all-electric cars, but because those cars have always been packed with cool features.

"Easter eggs" — frivolous little extras that Tesla throws in whenever it does software updates. Owners enjoy finding them.

The Model X's falcon-wing doors. Dramatic, slightly impractical, and a nightmare to manufacture. But Tesla has the only SUV on the road with such an exotic feature.

Bioweapon Defense Mode uses a powerful filtration system to render the interior air quality of the Model X or Model S "hospital grade," according to Tesla.

The Model X's 5,000-pound towing capacity. Nobody ever talks about it, but the Model X can tow a goodly amount for an electric SUV. It's very competitive with gas-powered SUVs that tout their capabilities.

The large, central portrait touchscreen on the Model S and Model X. This mega-tablet interface was a revelation when Tesla first introduced it on the Model S in 2012, but it's now emulated throughout the auto industry. It's actually canted slightly toward the driver.

Aero Wheels on the Model 3. The proprietary design is standard on the vehicle, enhancing airflow, reducing drag, and improving range.

Ludicrous Mode. The acceleration feature — which followed Insane Mode, first rolled for the all-wheel-drive Model S — enables Teslas to cover the 0-60 mph sprint at supercar-like velocities.

Frunks! All Teslas currently on sale have front trunks, expanding their cargo capacities. Having no gas engine helps to free up space.

Trunks! Teslas are commendable cargo haulers because they're effectively boxes on top of battery packs, creating ample space for luggage, groceries, of gear.

Quiet. In operation, Teslas are notably quiet and smooth, thanks to the optimization of airflow, solid build quality, and mostly silent electric motors.

The Tesla smartphone app. I've actually tested a number of these from assorted manufacturers, but Tesla's is the only one that's truly useful. For the Model 3, it replaces the traditional key fob.

The glass roof of the Model 3. It creates a stunning silhouette and floods the cabin with natural light.

The space-age operators' platform in the cab of the Tesla Semi. This space — clearly anticipating a time when semi-trucks drive themselves — is the most futuristic thing Tesla has ever designed.

Roadsters in space. CEO Elon Musk's personal Tesla Roadster was launched atop the SpaceX Falcon Heavy rocket in 2017, as a test payload. Piloted by "Starman," it set a new standard for automotive marketing.

Tesla's in-house audio system. Most luxury brands partner with a big-name audio company for premium sound systems, but Tesla developed its own — and it sounds absolutely fantastic.

Charge monitoring and mapping. Charging is among the most important things Tesla has to think about, so the company has made it a priority to track it in the vehicle and via the app, as well as to plot road-trip courses that use GPS navigation to permit island-hopping from charging location to charging location.

Navigate on Autopilot combines Tesla's GPS mapping system with Autopilot's ability to execute lane changes and freeway on- and off-ramping maneuvers.

The new Roadster's staggering performance specs. The all-new machine has a claimed 0-60 mph time of 1.9 seconds, making it the fastest production vehicle in the world.

The Model 3's consolidated vehicle-management system and central landscape touchscreen. Almost every aspect of the Model 3 is controlled here, and the traditional instrument cluster has been moved to the left side of the screen, and streamlined.

The radical design of the Cybertruck. In late 2019, Tesla had fallen into a design rut. The otherworldly, stainless-steel Cybertruck changed all that. Controversial to be sure, but also thrilling.

Read about the Cybertrucks' rad design.

Manufacturing simplicity. Electric cars are less complicated to build than gas-powered ones. Tesla has designed its factory in China to optimize this aspect of production, which could support and enviable profit margin for Tesla in the 20-30% range.

The white interior. It's an extra, but a very popular one. I was initially skeptical, but I'm now a fan. After all, it survived a 700-plus-mile family road trip!

Read about the road trip.

Over-the-air software updates. Just like smartphones, Teslas can be routinely upgraded while sitting in owners' driveways. This means that an older Tesla can acquire new features quite literally overnight.

The Supercharger network. Access to DC fast-charging used to be a lifetime perk for Tesla owners, but Tesla has begun to bill for the service. Still, it enables longer road trips and is completely integrated with each Tesla vehicle's systems.

Tesla's design philosophy. Head designer Franz von Holzhausen and Elon Musk argue that it doesn't cost anymore to make Teslas beautiful. But von Holzhausen has also exercised tasteful restraint, ensuring that Tesla's vehicles have a long market life.

Read about Franz's design influence.

Performance! Tesla vehicles have always combined electric virtuosity with industry-leading performance. Owners can usually expect to be driving one of the fastest cars on the road.

Battery design. Tesla has taken a complicated, multi-cell concept — thousands are wired together in packs — and perfected it, yielding impressive range and performance. The company also manufactures its own packs, in partnership with Panasonic.

The Model 3's minimalist driving experience. With the clean dashboard, you can focus on the road ahead. It's a blissful thing and my top Tesla feature.

The bottom line is that while plenty of other automakers put cool features in their cars, Teslas are crammed with ideas, ideas, and more ideas.

The rapper was arrested earlier this month after an altercation in Stockholm.

Joe Scarborough of "Morning Joe" on MSNBC took credit for the name, which reportedly angers Senate Majority Leader Mitch McConnell.

The couple has been married for 31 years.

When growth slows, Trump doubles down on old tax cut ideas.

Maybe they need a new assignment.

Archaic computer technology is hampering the effort to combat the effects of covid-19 in the United States.

oscon: RT @andreabledsoe: So proud our #womenintech resource center is live, w/ great articles +enter to win a #OSCON scholarship...

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Teacher question: You wrote recently that it was a good idea to teach comprehension skills, but our school district says we shouldn’t, that it’s prior knowledge that matters. Do you know the baseball study? Have you read Natalie Wexler’s research? It is really difficult to trust research when everyone tells us something different. Shanahan's response:  I feel your pain.

Teacher question: You wrote recently that it was a good idea to teach comprehension skills, but our school district says we shouldn’t, that it’s prior knowledge that matters. Do you know the baseball study? Have you read Natalie Wexler’s research? It is really difficult to trust research when everyone tells us something different. Shanahan's response:  I feel your pain.

Rick Bright, head of an agency tasked with developing coronavirus drugs, has said administration ignored his warnings.

China says it supports a World Health Organisation-led review into the global response to the coronavirus outbreak, but only "after the pandemic is over".

An alleged robber, who was wounded while his two suspected accomplices were killed in a shootout with police, has been arrested.

The Amateur Traveler talks to Photographer Ralph Velasco about two of his favorite cities in Spain, Toledo and Segovia. Both Toledo and Segovia are a short distance from Madrid and both offer interesting and historically significant cities with UNESCO World Heritage sites.

Hear about hiking the Kings Trail (Kungsleden) in Swedish Lapland as the Amateur Traveler talks to Agata from nullnfull.com about this northern and rugged portion of Sweden. Agata says, “Lapland is all about nature and wilderness”.

Hear about travel to New Caledonia as the Amateur Traveler talks to CJ Johnson from the MOVIELAND ABC Radio Show and Podcast about his recent trip to this part of France in the South Pacific.

After three decades of mystery, the cold case murder of a 17-year-old Ohio teen named Barbara Blatnik has been solved thanks in part to DNA genealogical research. The last time anyone saw Blatnik alive was around midnight on Dec. 19, 1987, when a friend dropped her off in Garfield Heights, Ohio. The following day, Blatnik,…

The post 32 Years After Teen’s Body Found Strangled on Side of Road, Suspect Finally Arrested appeared first on The Western Journal.

All it took was a pandemic, but the long-running joke finally became a reality: DEF CON is canceled. 

The annual hacker conference in Las Vegas, Nevada, typically draws tens of thousands of attendees to talk shop, compare notes, and generally cause delightful mischief. One element of that mischief is the constant — and until now false — yearly claim that DEF CON is canceled. The coronavirus changed that, and the organization announced Friday that the in-person conference will not take place this year. 

"The #DEFCONiscanceled meme has crossed over into real life, courtesy of #COVID19," read the announcement. "In early March we had hopes that things would be stable by August. That is no longer realistic." Read more...

Rita J. King, co-director and EVP for business development at Science House, recently conducted a series of interviews with business leaders, exploring the challenges and hurdles companies face in evolving business landscapes. In this interview, King chats with Craig Lemasters, CEO of Global Executive Group, about what companies face when navigating the digital transformation. They […]

Technology has come a long way, and cyber protection is the most crucial factor in advancing the future of digital defense. Interestingly enough, cybersecurity was not always an issue in backup and storage; but as technology advances, so do the methods of hackers. Saying this, let’s take a walk down memory lane. The 1900s: Where […]

The post How Backup And Storage Led To Cybercrime appeared first on Dumb Little Man.

Human remains located in Mulmur Township in March have been confirmed to be those of an 88-year-old Caledon man who was reported missing seven months ago.

The following article, Breaking: Texas AG Calls for Immediate Release of Salon Owner Jailed for Opening…Texas Lt Gov Offers to Pay Salon Owner’s $7k Fine, was first published on 100PercentFedUp.com.

Salon A La Mode owner Shelley Luther opened her salon in defiance of the lockdown order for salons to stay closed during the coronavirus crisis. She boldly ripped. up the citation she was given by the police and refused to shut her doors. Luther hired a lawyer and went to court where an activist judge […]

Continue reading: Breaking: Texas AG Calls for Immediate Release of Salon Owner Jailed for Opening…Texas Lt Gov Offers to Pay Salon Owner’s $7k Fine ...

The following article, Attorney for Jailed Dallas Salon Owner Shelley Luther Slams Obama Loyalist Judge as a “Tiny Tyrant”, was first published on 100PercentFedUp.com.

Warren Norred, attorney for Shelley Luther, joined Tucker Carlson to discuss the decision by an Obama loyalist Texas judge to jail his client for reopening her salon during the COVID-19 pandemic. Norred said the judge has exposed himself for the “tiny tyrant” that he is. The judge jailed Luther and even fined her $7,000. Texas […]

Continue reading: Attorney for Jailed Dallas Salon Owner Shelley Luther Slams Obama Loyalist Judge as a “Tiny Tyrant” ...

Manitoba’s unemployment rate nearly doubled between March and April, according to the monthly report from Statistics Canada released Friday morning.

NAD+ is a central metabolite participating in core metabolic redox reactions. The prokaryotic NAD synthetase enzyme NadE catalyzes the last step of NAD+ biosynthesis, converting nicotinic acid adenine dinucleotide (NaAD) to NAD+. Some members of the NadE family use l-glutamine as a nitrogen donor and are named NadEGln. Previous gene neighborhood analysis has indicated that the bacterial nadE gene is frequently clustered with the gene encoding the regulatory signal transduction protein PII, suggesting a functional relationship between these proteins in response to the nutritional status and the carbon/nitrogen ratio of the bacterial cell. Here, using affinity chromatography, bioinformatics analyses, NAD synthetase activity, and biolayer interferometry assays, we show that PII and NadEGln physically interact in vitro, that this complex relieves NadEGln negative feedback inhibition by NAD+. This mechanism is conserved in distantly related bacteria. Of note, the PII protein allosteric effector and cellular nitrogen level indicator 2-oxoglutarate (2-OG) inhibited the formation of the PII-NadEGln complex within a physiological range. These results indicate an interplay between the levels of ATP, ADP, 2-OG, PII-sensed glutamine, and NAD+, representing a metabolic hub that may balance the levels of core nitrogen and carbon metabolites. Our findings support the notion that PII proteins act as a dissociable regulatory subunit of NadEGln, thereby enabling the control of NAD+ biosynthesis according to the nutritional status of the bacterial cell.

27 September 2017

US policymakers should give special consideration to a more open immigration policy for highly skilled professionals from the EU. This would ultimately benefit the US economy.

Alfred C. O. Vertegaal
Dec 1, 2006; 5:2298-2310
Research

17 February 2020

Numerous zinc ectoenzymes are metalated by zinc and activated in the compartments of the early secretory pathway before reaching their destination. Zn transporter (ZNT) proteins located in these compartments are essential for ectoenzyme activation. We have previously reported that ZNT proteins, specifically ZNT5–ZNT6 heterodimers and ZNT7 homodimers, play critical roles in the activation of zinc ectoenzymes, such as alkaline phosphatases (ALPs), by mobilizing cytosolic zinc into these compartments. However, this process remains incompletely understood. Here, using genetically-engineered chicken DT40 cells, we first determined that Zrt/Irt-like protein (ZIP) transporters that are localized to the compartments of the early secretory pathway play only a minor role in the ALP activation process. These transporters included ZIP7, ZIP9, and ZIP13, performing pivotal functions in maintaining cellular homeostasis by effluxing zinc out of the compartments. Next, using purified ALP proteins, we showed that zinc metalation on ALP produced in DT40 cells lacking ZNT5–ZNT6 heterodimers and ZNT7 homodimers is impaired. Finally, by genetically disrupting both ZNT5 and ZNT7 in human HAP1 cells, we directly demonstrated that the tissue-nonspecific ALP-activating functions of both ZNT complexes are conserved in human cells. Furthermore, using mutant HAP1 cells, we uncovered a previously-unrecognized and unique spatial regulation of ZNT5–ZNT6 heterodimer formation, wherein ZNT5 recruits ZNT6 to the Golgi apparatus to form the heterodimeric complex. These findings fill in major gaps in our understanding of the molecular mechanisms underlying zinc ectoenzyme activation in the compartments of the early secretory pathway.