French police have dismantled smuggling networks trying to take advantage of a rush to buy medical supplies including face masks to tackle the coronavirus epidemic, the interior minister said Friday.

The Bcl-2 (B cell lymphoma 2)-related protein Nr-13 plays a major role in the regulation of cell death in developing avian B cells. With over 65% sequence similarity to the chicken Nr-13, herpesvirus of turkeys (HVT) vNr-13, encoded by the HVT079 and HVT096 genes, is the first known alphaherpesvirus-encoded Bcl-2 homolog. HVT-infected cells were reported to be relatively more resistant to serum starvation, suggested that vNr-13 could be involved in protecting the cells. Here, we describe CRISPR/Cas9-based editing of exon 1 of the HVT079 and HVT096 genes from the HVT genome to generate the mutant HVT-vNr-13 to gain insights into its functional roles. Overall, wild-type HVT and HVT-vNr-13 showed similar growth kinetics; however, at early time points, HVT-vNr-13 showed 1.3- to 1.7-fold-lower growth of cell-associated virus and 3- to 6.2-fold-lower growth of cell-free virus. In transfected cells, HVT vNr-13 showed a mainly diffuse cytoplasmic distribution with faint nuclear staining. Further, vNr-13 localized to the mitochondria and endoplasmic reticulum (ER) and disrupted mitochondrial network morphology in the transfected cells. In the wild-type HVT-infected cells, vNr-13 expression appeared to be directly involved in the disruption of the mitochondrial network, as the mitochondrial network morphology was substantially restored in the HVT-vNr-13-infected cells. IncuCyte S3 real-time apoptosis monitoring demonstrated that vNr-13 is unequivocally involved in the apoptosis inhibition, and it is associated with an increase of PFU, especially under serum-free conditions in the later stages of the viral replication cycle. Furthermore, HVT blocks apoptosis in infected cells but activates apoptosis in noninfected bystander cells.

IMPORTANCE B cell lymphoma 2 (Bcl-2) family proteins play important roles in regulating apoptosis during homeostasis, tissue development, and infectious diseases. Several viruses encode homologs of cellular Bcl-2-proteins (vBcl-2) to inhibit apoptosis, which enable them to replicate and persist in the infected cells and to evade/modulate the immune response of the host. Herpesvirus of turkeys (HVT) is a nonpathogenic alphaherpesvirus of turkeys and chickens that is widely used as a live vaccine against Marek’s disease and as recombinant vaccine viral vectors for protecting against multiple avian diseases. Identical copies of the HVT genes HVT079 and HVT096 encode the Bcl-2 homolog vNr-13. While previous studies have identified the potential ability of vNr-13 in inhibiting apoptosis induced by serum deprivation, there have been no detailed investigations on the functions of vNr-13. Using CRISPR/Cas9-based ablation of the vNr-13 gene, we demonstrated the roles of HVT vNr-13 in early stages of the viral replication cycle, mitochondrial morphology disruption, and apoptosis inhibition in later stages of viral replication.

Background and objectives

The effects of active case finding (ACF) models that mobilise community networks for early identification and treatment of tuberculosis (TB) remain unknown. We investigated and compared the effect of community-based ACF using a seed-and-recruit model with one-off roving ACF and passive case finding (PCF) on the time to treatment initiation and identification of bacteriologically confirmed TB.

Impaired insulin secretion from the pancreatic β-cells is central in the pathogenesis of type 2 diabetes (T2D), and microRNAs (miRNAs) are fundamental regulatory factors in this process. Differential expression of miRNAs contributes to β-cell adaptation to compensate for increased insulin resistance, but deregulation of miRNA expression can also directly cause β-cell impairment during the development of T2D. miRNAs are small noncoding RNAs that posttranscriptionally reduce gene expression through translational inhibition or mRNA destabilization. The nature of miRNA targeting implies the presence of complex and large miRNA–mRNA regulatory networks in every cell, including the insulin-secreting β-cell. Here we exemplify one such network using our own data on differential miRNA expression in the islets of T2D Goto-Kakizaki rat model. Several biological processes are influenced by multiple miRNAs in the β-cell, but so far most studies have focused on dissecting the mechanism of action of individual miRNAs. In this Perspective we present key islet miRNA families involved in T2D pathogenesis including miR-200, miR-7, miR-184, miR-212/miR-132, and miR-130a/b/miR-152. Finally, we highlight four challenges and opportunities within islet miRNA research, ending with a discussion on how miRNAs can be utilized as therapeutic targets contributing to personalized T2D treatment strategies.

Expect expansion plans by the graphics card giant

Trump's decision to air the selectively edited video clips led major US television networks to quickly cut away from his daily coronavirus briefing. 

Neither of the major football codes seem likely to emerge from the coronavirus outlook unscathed and nor do the free-to-air television networks that broadcast their matches.

Albert Gonzalez, 28, of Miami, Fla., was indicted today for conspiring to hack into computer networks supporting major American retail and financial organizations, and stealing data relating to more than 130 million credit and debit cards. Among the corporate victims named in the indictment are Heartland Payment Systems, a New Jersey-based card payment processor; 7-Eleven Inc., a Texas-based nationwide convenience store chain; and Hannaford Brothers Co. Inc., a Maine-based supermarket chain.

An international computer hacker pleaded guilty today to multiple charges relating to hacking activity and credit card fraud. More than 40 million credit and debit card numbers were stolen from major U.S. retailers as a result of the hacking activity. Albert Gonzalez, 28, of Miami, pleaded guilty today to 19 counts of conspiracy, computer fraud, wire fraud, access device fraud and aggravated identity theft relating to hacks into numerous major U.S. retailers.

Albert Gonzalez pleaded guilty to conspiracy to gain unauthorized access to the payment card networks operated by, among others, Heartland Payment Systems, 7-Eleven and Hannaford Brothers Co. Inc.

Albert Gonzalez, of Miami, the leader of the largest hacking and identity theft ring ever prosecuted by the U.S. government, has been sentenced to 20 years and one day in prison for his role in a series of hacks into a major payment processor and several retail networks.

A Pennsylvania man pleaded guilty today to charges stemming from his participation in a scheme to hack into computer networks and sell access to those networks.

A Pennsylvania man was sentenced to serve 18 months in prison for his role in a scheme to hack into computer networks and sell access to those networks.

CSED Working Paper No. 33: Physician Social Networks and Geographic Variation in Medical Care

Media networks have paid billions for live sports that they aren't going to broadcast this year. But nobody's pressing the issue yet, as the cable industry is focused on survival.

Tamil Nadu government announced that it had directed all blood banks in the state to use the blood donation feature on Facebook to reach out to the voluntary donors.

World Trade Center is a global network of office complexes that has so far connected 330 cities in 100 countries across the world. It is managed and regulated by renowned World Trade Center Association...

This book introduces the concept of knowledge networks and markets (KNMs) in the life sciences, the emerging organisations and mechanisms to share and trade an increasing variety of knowledge assets.

London Underground and other locally run services were excluded from previous coronavirus aid packages

At a time when more transactions than ever are happening online, payments behemoth Stripe is announcing three new features to continue expanding its reach. The company today announced that it will now offer card issuing services directly to businesses to let them in turn make credit cards for customers tailored to specific purposes. Alongside that, it’s going […]

Nvidia today announced its plans to acquire Cumulus Networks, an open-source-centric company that specializes in helping enterprises optimize their data center networking stack. Cumulus offers both its own Linux distribution for network switches, as well as tools for managing network operations. With Cumulus Express, the company also offers a hardware solution in the form of […]

She's brought Hollywood prestige to The Sunday Project with a series of exclusive interviews.

Interference hunting in TDD networks

5G will help in easing out on the network congestion thereby improving user experience while streaming videos across platforms. It will also provide a smart data pipe for content; monetize broadcast spectrum and provide live streaming on-the-go sans data caps.

New York : Apress, [2019]

Singapore : Springer, [2019]

Cham : Springer, 2019.

Cham : Springer, 2019.

Cham : Springer, 2019.

Singapore : Springer, c2019.

Cham, Switzerland : Springer, 2019.

Cham : Springer, c2019.

Cham, Switzerland : Springer, [2019]

Cham : Springer, 2019.

Cham, Switzerland : Springer, [2019]

IL&FS Transportation Networks

Amarís, Hortensia, author

Shih, Clara Chung-wai

Displaying your social media network activity on your website can have tremendous advantages. It shows your readers that you’re an active participant in your niche and helps you establish yourself as a thought leader. The best part is that displaying your activity is fast and easy in WordPress.

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Displaying your social media network activity on your website can have tremendous advantages. It shows your readers that you’re an active participant in your niche and helps you establish yourself as a thought leader. The best part is that displaying your activity is fast and easy in WordPress.

complete article

Despite the huge popularity of social networking among teens, more respondents reported using Gmail than Snapchat or Facebook, June 2016 research uncovered. The only site used more than Gmail was YouTube, which is not surprising given the plethora of content and influencers available on the video-sharing site.

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Registering to vote just got a whole lot easier for tens of million social media users in the U.S. At least six major social media apps are launching voter registration drives to meet young voters exactly where they spend much of their time: on their phones.

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