location USING RESOURCE ELEMENT LOCATION PATTERN TO CONVEY MCS OF CONTROL CHANNEL By www.freepatentsonline.com Published On :: Thu, 29 Jun 2017 08:00:00 EDT A mechanism that allows the successful decoding of MCS information of cell edge UEs while retaining the performance for the other UEs of the cell is provided. In one aspect, a UE may determine an uplink control coding rate based on an uplink signal quality. The UE may encode uplink control data based on the uplink control coding rate. The UE may apply a pattern of unused resource element locations in uplink control resource elements based on the uplink control coding rate. The UE may transmit the uplink control resource elements with the pattern of unused resource element locations. In another aspect, an eNB may receive uplink control resource elements. The eNB may determine an uplink control coding rate based on a pattern of resource element locations in the uplink control resource elements. The eNB may decode uplink control data based on the uplink control coding rate. Full Article
location METHODS AND APPARATUS FOR MULTIPLE USER UPLINK BANDWIDTH ALLOCATION By www.freepatentsonline.com Published On :: Thu, 29 Jun 2017 08:00:00 EDT Methods and apparatus for multiple user uplink are provided. In one aspect, method for wireless communication includes receiving an assignment of a frequency bandwidth for an uplink transmission of a station. The method further includes determining whether a portion of the assigned frequency bandwidth is unavailable for the uplink transmission. The method further includes selectively transmitting the uplink transmission based on whether the portion of the assigned frequency bandwidth is unavailable. Full Article
location SLOT ALLOCATION IN TIME DIVISION DUPLEX SYSTEMS By www.freepatentsonline.com Published On :: Thu, 29 Jun 2017 08:00:00 EDT Various communication systems may benefit from managing signal interference. For example, certain wireless communication systems may benefit from a dynamic time division duplex system involving slot allocation. A method includes allocating, by an access point, in a time division duplex frame a plurality of radio resource slots, each one of the plurality of radio resource slots being allocated for a downlink or an uplink transmission, and determining that the allocation of the downlink or uplink transmission should be changed. The method also includes applying a permutation pattern to re-allocate at least one of the plurality of radio resource slots for the downlink or uplink transmission. Full Article
location MULTIPLE NETWORK ALLOCATION VECTOR OPERATION By www.freepatentsonline.com Published On :: Thu, 29 Jun 2017 08:00:00 EDT A first wireless device may determine a bandwidth for transmitting a frame, calculate two or more Spatial Reuse (SR) parameter values for the bandwidth, set, using the SR parameter values, first and second SR fields of the frame based on the bandwidth and a channel center frequency in which the bandwidth is carried, and transmit the frame to a second wireless device on the bandwidth. The first and second SR fields may be set to a first value when the bandwidth is a 40 MHz bandwidth and the channel center frequency is in a 2.4 GHz band. The first and second SR fields may be set to the first value when the bandwidth is an 80+80 MHz bandwidth and the channel center frequency is in a 5 GHz band. The first value may be a minimum of SR parameter values for first and second bandwidths in the bandwidth. Full Article
location Handle mechanism with multiple stop locations and a lawn mower including the handle mechanism By www.freepatentsonline.com Published On :: Tue, 26 May 2015 08:00:00 EDT A handle mechanism with multiple stop locations and a lawn mower including the handle mechanism includes a handle and a location shifting element with multiple stop locations, the handle being provided with a locking mechanism that is automatically clamped with one of the multiple of stop locations of the location shifting element for fixing or adjusting the handle. A lawn mower, including the handle mechanism, includes a lawn mower body, the handle rotatably connected with the lawn mower body by the handle rotating shaft. The locking mechanism and the location shifting element are equipped with stop locations can be automatically locked, therefore, the operator can adjust the stop locations of the lawn mower conveniently. Full Article
location Method and Device for Chronologically Synchronizing a Kinematic Location Network By www.freepatentsonline.com Published On :: Thu, 15 Jun 2017 08:00:00 EDT Methods and devices are presented for synchronizing positioning signals in a kinematic location network. In particular, methods and devices are presented for synchronizing a unique positioning signal generated by a positioning-unit device to a reference positioning signal generated by a reference transmitter, where the positioning-unit device and the reference transmitter are moving relative to each other. In certain embodiments the reference transmitter or the positioning-unit device, or both, self-monitor trajectory data comprising one or more of location, velocity or acceleration, e.g. using inertial navigation systems, and broadcast that data in their positioning signals. The trajectory data enables estimation of Doppler shifts and propagation delays associated with the positioning signals, allowing measurement and correction of clock drift for synchronization of the positioning signals. Full Article
location APPARATUS FOR IDENTIFYING LOCATION OF USER By www.freepatentsonline.com Published On :: Thu, 22 Jun 2017 08:00:00 EDT A terminal for identifying a location of a user includes a transmitter configured to transmit data, a receiver configured to receive the data, a sensor configured to sense at least one piece of context information, and a controller configured to determine a priority order of area information, service set identifier (SSID) information, global positioning system (GPS) signal information, and the context information corresponding to each location, identify the location based on the priority order, and determine the priority order of the SSID information and the GPS signal information in response to retrieving a number of SSIDs. Full Article
location Señor Sisig Now Has an Oakland Location By www.eastbayexpress.com Published On :: Fri, 17 Apr 2020 04:00:00 -0700 Just in time to celebrate its ten-year anniversary, popular Filipino-fusion food truck Señor Sisig has established a long-term location in Oakland. The brand-new spot is at 3331 Fruitvale Ave. in the Dimond District.… Full Article
location BATTING PAD FOR ADJUSTING LOCATION OF BAT IN BATTER'S HAND By www.freepatentsonline.com Published On :: Thu, 29 Jun 2017 08:00:00 EDT A batting pad comprising a body made from a cushioning material and having a ring portion adapted to fit over a batter's thumb and an appendage to cushion the palm area between a batter's thumb and index finger. The appendage has a thickness T at its thickest point located between the first end and the distant end, and the thickness T of the appendage is selected to optimize an angle of a bat in the batter's hand relative to a line parallel to the batter's forearm, the angle being determined by the thickness T of the appendage displacing the bat toward the tip of the batter's index finger and causing the bat to contact the palm of the batter's hand near the batter's little finger. Full Article
location Touchwood named top cycling location By thebirminghampress.com Published On :: Tue, 18 Mar 2014 12:12:57 +0000 Award recognises centre's bike-friendly credentials. Full Article Business Environment Solihull Top Cycling Location Award touchwood
location Premier location for Midlands Media Awards By thebirminghampress.com Published On :: Thu, 18 Apr 2013 11:22:36 +0000 The Midlands Media Awards is this year to take place at The Holte Suite, Aston Villa FC’s prestigious hospitality centre. Full Article Birmingham Business Uncategorized Aston Villa awards the media
location Dabur India resumes production at all manufacturing locations By retail.economictimes.indiatimes.com Published On :: 2020-05-06T08:23:06+05:30 In view of the fresh guidelines issued by the Ministry of Home Affairs on May 1 and after evaluating the ground situation, the company has resumed production at all manufacturing locations, Dabur India said in a regulatory filing. Full Article
location Access To Illinois Dentists Varies Across Locations And Incomes By www.northernpublicradio.org Published On :: Tue, 29 Jan 2019 12:00:00 +0000 For some people, going to the dentist can be a double-edged sword. On one hand, regular dental treatment keeps teeth healthy. But at the same time, the sights, sounds and smells of a dental office can be unsettling for some. But which dentists you can access, and even afford, often depends on where you live. Dionne Haney is Director of Professional Services for the Illinois State Dental Society, a professional group affiliated with the American Dental Association. Haney says membership records can provide a tally of dental specialists. "We believe there's currently about 11,000 dentists licensed in the state," she said. "Approximately 8,500 of them actually practice. But with regards to membership in ISDS, they can be practicing or retired." That may seem like a lot of dentists, but Haney says they're spread across a state that has more than 12 million residents. About two-thirds of practicing Illinois dentists are based in and around Chicago. This doesn't mean people have no access to Full Article
location Temporary Location Chosen For Rockford's Family Peace Center By www.northernpublicradio.org Published On :: Thu, 23 Jan 2020 23:05:02 +0000 Listen, believe, provide. That's the mission of Rockford's Family Peace Center. It will provide safety and services for survivors of violence, sexual assault, child abuse, elder or dependent abuse, and human trafficking. Rockford Mayor Tom McNamara announced that "Phase 1" of the center will open in the next six months. It will be located on North Main Street, across from the Coronado Theater. He said, "We are terribly excited to create a safe space that survivors can get all the services they need -- and not just the services they need, but the hope they need -- that tomorrow's going to be a better day for them and their family." Judy Moe serves on a committee that represents a variety of religious organizations who work together to help survivors. She said they have been meeting for several months so they can warmly welcome and help any person who enters the door. "We are going to work with people when they come in and do it in a way that is non-threatening." She continued, "We will Full Article
location Economic Recovery May Vary By Location: NRF Chief Economist By www.rttnews.com Published On :: Fri, 08 May 2020 10:51:41 GMT The National Retail Federation's Chief Economist Jack Kleinhenz believes the economic recovery in the United States from the Coronavirus crisis would come gradually and may vary by location depending on the severity. His remark comes as more and more stores are reopening across the country after at least 30 U.S. states began easing lockdown restrictions and decided to reopen businesses... Full Article
location Drought assistance allocation under review as council seeks to reject $1m funding By www.abc.net.au Published On :: Mon, 30 Sep 2019 15:28:00 +1000 A regional council looks set to hand back $1 million in drought funding as the Federal Government concedes it needs to review how it hands out assistance to communities battling dry weather. Full Article ABC South West Victoria southwestvic Disasters and Accidents:All:All Disasters and Accidents:Drought:All Government and Politics:All:All Government and Politics:Federal Government:All Rural:All:All Australia:All:All Australia:VIC:All Australia:VIC:Port Fairy 3284
location WA Police taser data reveals the locations where tasers are used most in the state By www.abc.net.au Published On :: Wed, 23 Oct 2019 10:50:00 +1100 One West Australian is tasered the equivalent of every weekday by police officers, new data obtained by the ABC via Freedom of Information shows. Full Article ABC Radio Perth perth Law Crime and Justice:All:All Law Crime and Justice:Police:All Australia:WA:All Australia:WA:Perth 6000
location SA irrigators receive full water allocation By www.abc.net.au Published On :: Tue, 14 May 2019 12:57:00 +1000 Full Article ABC Riverland riverland Environment:Rivers:All Environment:Rivers:Murray-Darling Basin Environment:Water Management:All Environment:Water Supply:All Australia:SA:Paringa 5340
location Edie's future location By www.abc.net.au Published On :: Fri, 09 Aug 2019 11:12:00 +1000 Full Article 639 ABC North and West northandwest Business Economics and Finance:Industry:All Business Economics and Finance:Industry:Building and Construction Business Economics and Finance:Industry:Rail Transport Community and Society:All:All Community and Society:Community Organisations:All Community and Society:History:All Rural:All:All Rural:Community Development:All Australia:SA:All Australia:SA:Burra 5417
location Chit Chat on location with The Heroes By www.abc.net.au Published On :: Fri, 20 Sep 2019 06:40:00 +1000 Full Article 1233 ABC Newcastle newcastle Arts and Entertainment:Film (Movies):Documentary Arts and Entertainment:Music:Rock Business Economics and Finance:Industry:Film Community and Society:History:All Australia:NSW:Newcastle 2300
location App links food charities to businesses ensuring deliveries to the hungry in remote locations By www.abc.net.au Published On :: Fri, 07 Jun 2019 07:39:00 +1000 Charities are turning to technology as they struggle to source enough food to feed a growing number of people going hungry in rural and remote communities. Full Article ABC Eyre Peninsula and West Coast adelaide southeastsa eyre Community and Society:All:All Community and Society:Charities:All Community and Society:Charities and Community Organisations:All Community and Society:Regional:All Australia:SA:All Australia:SA:Gawler 5118 Australia:SA:Millicent 5280 Australia:SA:Mount Gambier 5290 Australia:SA:Murray Bridge 5253 Australia:SA:Port Adelaide 5015 Australia:SA:Port Lincoln 5606 Australia:SA:Victor Harbor 5211
location Pelican lover, Liz Murphy, wanted to know why they only flock to certain inland locations By www.abc.net.au Published On :: Thu, 19 Oct 2017 06:42:00 +1100 Full Article ABC Local centralwest Education:Subjects:Environment Environment:Environmental Impact:All Environment:Environmental Management:All Human Interest:People:All Science and Technology:Animals:Birds Australia:NSW:Forbes 2871 Australia:NSW:Orange 2800
location North Queensland's oldest residential building moves locations to make way for gold mine expansion By www.abc.net.au Published On :: Fri, 01 Nov 2019 06:25:00 +1100 In the north-west Queensland town of Ravenswood, two heritage-listed buildings at a school have been moved due to an expansion of the local gold mine. Full Article ABC North Queensland northqld Business Economics and Finance:Industry:Gold Business Economics and Finance:Industry:Mining Community and Society:History:All Education:Schools:Primary Schools Education:Schools:Public Schools Rural:Mining:All Australia:QLD:Ravenswood 4816 Australia:QLD:Townsville 4810
location Fire fighters assessing the situation at the location of a housefire in Alice Springs By www.abc.net.au Published On :: Thu, 13 Jun 2019 10:48:00 +1000 Full Article 783 ABC Alice Springs alicesprings Disasters and Accidents:All:All Disasters and Accidents:Fires:All Australia:NT:Alice Springs 0870
location Grape company's gripe with NT Government over water allocation By www.abc.net.au Published On :: Tue, 16 Jul 2019 08:40:00 +1000 One of Australia's biggest table grape companies is threatening to rip up vines and scrap its million-dollar plan to expand in the Northern Territory. Full Article 783 ABC Alice Springs alicesprings Business Economics and Finance:Trade:All Environment:Water Management:All Rural:Agricultural Crops:All Rural:All:All Rural:Irrigation:All Australia:NT:Alice Springs 0870 Australia:NT:Ti Tree 0872
location Water allocations for western NSW reduced as drought tightens its grip By www.abc.net.au Published On :: Mon, 01 Jul 2019 13:43:00 +1000 Water allocations for farmers and communities in western NSW are reduced in new plans released today. Full Article ABC Central West NSW brokenhill westernplains centralwest newengland riverina Disasters and Accidents:Drought:All Environment:Water:All Environment:Water Management:All Environment:Water Supply:All Australia:NSW:Broken Hill 2880 Australia:NSW:Dubbo 2830 Australia:NSW:Orange 2800 Australia:NSW:Tamworth 2340 Australia:NSW:Wagga Wagga 2650
location Greenrock On Relocation Plan For Sea Turtles By bernews.com Published On :: Wed, 10 May 2017 12:29:36 +0000 “Greenrock was surprised by the announcement to capture and relocate sea turtles from the Great Sound for May and June, it is certainly an interesting initiative, and one that raises some questions for consideration,” Greenrock Executive Director Jonathan Starling said. Earlier this month the Government said that in “anticipation of intense boating activity in the […](Click to read the full article) Full Article All Environment News #AmericasCup #Greenrock #TurtleMoveAC #Turtles
location Additional Information On Sea Turtle Relocation By bernews.com Published On :: Wed, 10 May 2017 20:49:06 +0000 Starting this week, America’s Cup Bermuda [ACBDA], in cooperation with the Bermuda Aquarium, Museum and Zoo [BAMZ], are coordinating the collection of sea turtles in anticipation of increased marine traffic in the Great Sound over May and June. “It is well known in the sea turtle conservation community that where there are turtles and boats, […](Click to read the full article) Full Article All Environment News #AmericasCup #TurtleMoveAC #Turtles
location BEST: ‘Ethical Dilemmas’ With Turtle Relocation By bernews.com Published On :: Fri, 12 May 2017 13:02:11 +0000 [Updated] “The planned temporary relocating of turtles — certainly with the turtles’ interest at heart — from the America’s Cup main racecourse, poses a number of environmental and ethical dilemmas,” BEST said today. It was previously announced that in “anticipation of intense boating activity in the Great Sound,” the America’s Cup Bermuda is “coordinating a […](Click to read the full article) Full Article All Environment News #AmericasCup #BermudaEnvironmentalAndSustainabilityTaskforce #BEST #TurtleMoveAC #Turtles
location Covid-19: Bill To Allow Power To Close Locations By bernews.com Published On :: Mon, 16 Mar 2020 16:11:47 +0000 A Bill to provide power for the Minister of Health to prevent the threatened spread of a communicable disease by “closing schools, cinemas, churches, hotels or other places of public resort” was tabled in the House Of Assembly today. The Bill’s Explanatory Memorandum states, “This Bill seeks to amend the Public Health Act 1949 to […](Click to read the full article) Full Article All News Politics #BermudaLaws #BermudaPolitics #Covid19 #Health
location A Tour of the Twilight Filming Locations By www.jamieism.com Published On :: Mon, 23 Oct 2017 08:00:03 +0000 I’ve been to Forks and La Push, where the Twilight book series takes place, but I’ve never been to the actual filming locations from the movies! That’s because while the director wanted to film in Forks, when he came to scout the town he realized there was just not enough infrastructure to support an entire film crew– there were literally not enough places to house and feed all of the people who would be working on the movie, and there was no other large town within driving distance! (Yeah, Forks is really far out there) Enter Halloweento— I mean, St. Helens, Oregon! St. Helens stands in for most of Forks in the movies, and a few places in nearby Portland, Oregon make up most of the rest. I ended up nearby by complete accident, as it was only when I picked up a brochure at Halloweentown that I learned that Bella’s house was just on the other side of town! LUCKY!!! So, we took a short break to drive over to the locations that were still standing, and made a special detour to Portland because how could we leave the Cullen house behind?! I WANTED TO GO INSIDE SO BADLY! It is a real house with real people that live there, and although they encouraged taking photos from outside (according both to a brochure from City Hall and a sign in front of the house), the inside was off-limits. Honestly, it’s not Bella that I particularly love. I didn’t care for her portrayal by Kristen Stewart (sorry!), or her melancholy, passive attitude in the book. Actually, I didn’t care for Edward, either. It was the idea of a love so strong that forces of nature were pulling you together. It’s the idea of fitting together so perfectly that you can’t do anything without the other that I am a complete sucker for! I said it on my old Livejournal years ago, but Twilight is just a big Mary Sue anyway, so I like to imagine myself as Bella, with a gorgeous (female) Edward out there waiting for me. ???? Like I said, it’s the love, not the characters themselves. I’ll fight you on this (just kidding). ???? Here is where Edward rescued Bella from some catcallers, complete with the mural that the movie crew painted (!) on the building. And the theater that they drove past, that you can’t really see well in the movie but hey, it was on the map!! This is Jilly’s, which supplied all of the dresses in the shop when Bella went to pick out something with her friends for prom. Oddly enough, they had this sign outside but no actual merchandise inside. Go figure. ???? This place, which is a private house now, was the bookstore where Bella went to find out more information about the Quileute myths. And then, though we only had a little bit of time, we drove over to Portland and checked out Edward’s house. I have to say…. THIS HOUSE WAS GORGEOUS. Whew! I would LOVE to live there. LOVE LOVE LOVE! I would also have loved a tour, but it is a private residence, and therefore we kept our distance. Mmm, but it sure is gorgeous! Twilight brings back a lot of memories for me, and it’s special because I grew up (mostly) in Washington. It’s also not the only book series to claim that there are werewolves living amongst the local residents. The town that I spent most of my childhood in also has a series revolving around it! I’m planning to take a trip over there to see my old friends and photograph all of the wolfy places of interest, so I’ll post about it then! Love ya, and see ya tomorrow!(1,343 geeks have read this) Full Article ☆ being a dork ☆ books ☆ i heart travel ☆ i heart washington ☆ movies ☆ photos
location IBM injects analytics into data centre consolidation and relocation services By www.ibm.com Published On :: Wed, 09 Mar 2011 02:02:42 GMT IBM today announced a new analytics-based tool for its data centre consolidation and relocation services which help clients gain up to 50% operational savings from the consolidation of IT assets and data centres. Full Article Global Technology Services
location Localz wins IBM SmartCamp Australia 2015 with innovative micro-location technology By www.ibm.com Published On :: Thu, 17 Sep 2015 01:07:35 GMT IBM (NYSE: IBM) today announced Melbourne start-up Localz as the winner of IBM SmartCamp Australia 2015, a global competition that brings together entrepreneurs, investors and experienced mentors. Localz provides software that connects the digital and physical worlds for enterprise, using the latest micro-location technology to enhance customer’s in-store experience, improve asset and inventory tracking and provide frictionless mobile payments, at scale. One of the key differentiators of the solution is that it works across different technologies (iBeacons, Bluetoth Low Energy, GPS or even QR Codes). Full Article Corporate
location Location manager By fun-movie-trivia.blogspot.com Published On :: Fri, 14 Sep 2012 01:44:00 +0000 Full Article Filmmaking Terms
location RFP now open for Fire Protection Research Foundation project on “Combustible Gas Distribution in Buildings and Detector Location Analysis” By community.nfpa.org Published On :: 2019-08-27T14:26:24Z Recent experiences with combustible gas releases in residential buildings have led to a proposal for NFPA Standards Development for locating combustible gas detectors and consensus on installation location requirements. NFPA is considering a proposed Full Article building & life safety research fire protection system researchers
location Amoeba Music will begin construction on new location in 'next week or so,' says owner By www.latimes.com Published On :: Wed, 22 Apr 2020 19:20:04 -0400 Despite the launch of a GoFundMe page, Amoeba Music co-owner Jim Henderson says that construction on their new space will begin shortly, with a fall opening anticipated. Full Article
location UK holidays: The best hiking locations in the UK for post-lockdown breaks By www.express.co.uk Published On :: Sat, 09 May 2020 06:01:00 +0100 UK HOLIDAYS have become even more popular as holidaymakers ditch their summer holiday plans for future staycation breaks. Here's a list of the best hiking destinations in the UK to get you ready for that post-lockdown break. Full Article
location Two more Lou Malnati's Pizzeria locations are headed to Indy's north side By rssfeeds.indystar.com Published On :: Fri, 24 Apr 2020 15:47:00 +0000 In March, Lou Malnati's confirmed its first location opening in Indianapolis. Now one is set for Carmel and another for Broad Ripple. Full Article
location Portillo's makes its Hendricks County debut as Avon location opens By rssfeeds.indystar.com Published On :: Mon, 24 Sep 2018 12:50:25 +0000 Italian beef and Chicago-style hot dogs are now being sold near Ronald Reagan Parkway and U.S. 36. Full Article
location Indiana BMV is reopening locations. Here's what you need to know. By rssfeeds.indystar.com Published On :: Fri, 08 May 2020 21:05:04 +0000 Locations of the Indiana Bureau of Motor Vehicles are reopening to offer services that can be provided only inside a branch. Full Article
location Amazon’s autonomous robots have started delivering packages in a new location: Southern California By www.washingtonpost.com Published On :: Mon, 12 Aug 2019 19:30:22 +0000 After nearly eight months of knowledge-gathering street tests and thousands of successful deliveries, Amazon has announced that its delivery robots have begun delivering packages to customers in Irvine, Calif. Full Article
location Council votes against proposed cannabis store location in Lakeshore By windsor.ctvnews.ca Published On :: Fri, 8 May 2020 16:54:00 -0400 In Lakeshore, it may be a little while longer before a retail cannabis store opens. Full Article
location N{alpha}-Acetylation of the virulence factor EsxA is required for mycobacterial cytosolic translocation and virulence [Molecular Bases of Disease] By feedproxy.google.com Published On :: 2020-04-24T06:08:45-07:00 The Mycobacterium tuberculosis virulence factor EsxA and its chaperone EsxB are secreted as a heterodimer (EsxA:B) and are crucial for mycobacterial escape from phagosomes and cytosolic translocation. Current findings support the idea that for EsxA to interact with host membranes, EsxA must dissociate from EsxB at low pH. However, the molecular mechanism by which the EsxA:B heterodimer separates is not clear. In the present study, using liposome-leakage and cytotoxicity assays, LC-MS/MS–based proteomics, and CCF-4 FRET analysis, we obtained evidence that the Nα-acetylation of the Thr-2 residue on EsxA, a post-translational modification that is present in mycobacteria but absent in Escherichia coli, is required for the EsxA:B separation. Substitutions at Thr-2 that precluded Nα-acetylation inhibited the heterodimer separation and hence prevented EsxA from interacting with the host membrane, resulting in attenuated mycobacterial cytosolic translocation and virulence. Molecular dynamics simulations revealed that at low pH, the Nα-acetylated Thr-2 makes direct and frequent “bind-and-release” contacts with EsxB, which generates a force that pulls EsxB away from EsxA. In summary, our findings provide evidence that the Nα-acetylation at Thr-2 of EsxA facilitates dissociation of the EsxA:B heterodimer required for EsxA membrane permeabilization and mycobacterial cytosolic translocation and virulence. Full Article
location SSO and other putative inhibitors of FA transport across membranes by CD36 disrupt intracellular metabolism, but do not affect FA translocation [Research Articles] By feedproxy.google.com Published On :: 2020-05-01T00:05:28-07:00 Membrane-bound proteins have been proposed to mediate the transport of long-chain FA (LCFA) transport through the plasma membrane (PM). These proposals are based largely on reports that PM transport of LCFAs can be blocked by a number of enzymes and purported inhibitors of LCFA transport. Here, using the ratiometric pH indicator (2',7'-bis-(2-carboxyethyl)-5-(and-6-)-carboxyfluorescein and acrylodated intestinal FA-binding protein-based dual fluorescence assays, we investigated the effects of nine inhibitors of the putative FA transporter protein CD36 on the binding and transmembrane movement of LCFAs. We particularly focused on sulfosuccinimidyl oleate (SSO), reported to be a competitive inhibitor of CD36-mediated LCFA transport. Using these assays in adipocytes and inhibitor-treated protein-free lipid vesicles, we demonstrate that rapid LCFA transport across model and biological membranes remains unchanged in the presence of these purported inhibitors. We have previously shown in live cells that CD36 does not accelerate the transport of unesterified LCFAs across the PM. Our present experiments indicated disruption of LCFA metabolism inside the cell within minutes upon treatment with many of the "inhibitors" previously assumed to inhibit LCFA transport across the PM. Furthermore, using confocal microscopy and a specific anti-SSO antibody, we found that numerous intracellular and PM-bound proteins are SSO-modified in addition to CD36. Our results support the hypothesis that LCFAs diffuse rapidly across biological membranes and do not require an active protein transporter for their transmembrane movement. Full Article
location Commentary on SSO and other putative inhibitors of FA transport across membranes by CD36 disrupt intracellular metabolism, but do not affect fatty acid translocation [Commentaries] By feedproxy.google.com Published On :: 2020-05-01T00:05:27-07:00 Full Article
location P1 allocation results to be mailed By www.news.gov.hk Published On :: Thu, 23 Apr 2020 00:00:00 +0800 To reduce social contact in light of the COVID-19 epidemic, the Primary One Central Allocation results will be posted to parents. Announcing the move today, the Education Bureau said it will deliver door-to-door the Primary One Registration Form with Central Allocation results to parents from June 3 to 4 through Hongkong Post’s Local CourierPost service. If no one is present to receive the item at the time of delivery, a mail collection notification card will be left for parents to collect it from the designated post office from the afternoon of the following working day. If parents have not received the Primary One Registration Form or the notification card by June 5, they can collect the registration form at the designated Collection Centre from June 6 to 7. The Education Bureau will send letters to parents tomorrow to notify them of the arrangements. Parents can get updates on the latest arrangements for the release of Central Allocation results and registration through the bureau’s press releases and messages posted on its website. Call 2891 0088 for information on Primary One admission. For further enquiries, contact the bureau's School Places Allocation Section (Primary One Admission) on 2832 7700 or 2832 7740. Full Article
location N{alpha}-Acetylation of the virulence factor EsxA is required for mycobacterial cytosolic translocation and virulence [Molecular Bases of Disease] By www.jbc.org Published On :: 2020-04-24T06:08:45-07:00 The Mycobacterium tuberculosis virulence factor EsxA and its chaperone EsxB are secreted as a heterodimer (EsxA:B) and are crucial for mycobacterial escape from phagosomes and cytosolic translocation. Current findings support the idea that for EsxA to interact with host membranes, EsxA must dissociate from EsxB at low pH. However, the molecular mechanism by which the EsxA:B heterodimer separates is not clear. In the present study, using liposome-leakage and cytotoxicity assays, LC-MS/MS–based proteomics, and CCF-4 FRET analysis, we obtained evidence that the Nα-acetylation of the Thr-2 residue on EsxA, a post-translational modification that is present in mycobacteria but absent in Escherichia coli, is required for the EsxA:B separation. Substitutions at Thr-2 that precluded Nα-acetylation inhibited the heterodimer separation and hence prevented EsxA from interacting with the host membrane, resulting in attenuated mycobacterial cytosolic translocation and virulence. Molecular dynamics simulations revealed that at low pH, the Nα-acetylated Thr-2 makes direct and frequent “bind-and-release” contacts with EsxB, which generates a force that pulls EsxB away from EsxA. In summary, our findings provide evidence that the Nα-acetylation at Thr-2 of EsxA facilitates dissociation of the EsxA:B heterodimer required for EsxA membrane permeabilization and mycobacterial cytosolic translocation and virulence. Full Article
location First School Allocation Exercise 2020 invites applications for five kindergarten premises in public housing estates By www.info.gov.hk Published On :: Mon, 06 Jan 2020 12:09:03 Full Article
location Application deadline extended for First School Allocation Exercise 2020 for allocation of five new estate kindergarten premises By www.info.gov.hk Published On :: Thu, 30 Jan 2020 17:58:19 Full Article
location New notification arrangements on Secondary One discretionary places and distribution of school choice documents for Central Allocation By www.info.gov.hk Published On :: Tue, 24 Mar 2020 15:51:38 Full Article