hp An Inflection Point in High-Throughput Proteomics with Orbitrap Astral: Analysis of Biofluids, Cells, and Tissues - ACS Publications By news.google.com Published On :: Tue, 20 Aug 2024 07:00:00 GMT An Inflection Point in High-Throughput Proteomics with Orbitrap Astral: Analysis of Biofluids, Cells, and Tissues ACS Publications Full Article
hp Elevator at Southport Temporarily Out-of-Service (Elevator Status) By www.transitchicago.com Published On :: (Wed, Oct 30 2024 11:01 AM to TBD) The Loop- bound platform elevator at Southport (Brown Line) is temporarily out-of-service due to elevator upgrades. Full Article
hp Elevator at Southport Temporarily Out-of-Service (Elevator Status) By Published On :: Wed, 30 Oct 2024 11:01:33 GMT (Wed, Oct 30 2024 11:01 AM to TBD) The Loop- bound platform elevator at Southport (Brown Line) is temporarily out-of-service due to elevator upgrades. Full Article
hp Nationwide 350 hp Boiler Tests at Ultra-Low NOx By www.achrnews.com Published On :: Mon, 18 Sep 2023 08:30:00 -0400 The third-party test was conducted at the Superior Boiler factory in Hutchison, Kansas, by Montrose Air Quality Services. Full Article
hp Nationwide 250 hp Boiler Achieves 5 ppm NOx Performance By www.achrnews.com Published On :: Wed, 26 Jul 2023 12:30:00 -0400 Equipped with an Oilon LN30 ultra-low NOx burner and Nationwide’s Eagle PLC-based Control System, the 250 hp package boiler achieved average emissions performance of 5 ppm NOx and 0 ppm CO (corrected to 3% O2) during third-party testing. Full Article
hp Schools Save Energy with CHP By www.achrnews.com Published On :: Mon, 03 Aug 2015 07:31:00 -0400 While cogeneration is still largely an industrial process, the market has significant potential for educational institutions coast to coast. Full Article
hp Episode 42: Interview Gregor Hohpe By feedproxy.google.com Published On :: Sun, 07 Jan 2007 10:13:11 +0000 In this episode, Gregor Hohpe gives us a great introduction to enterprise messaging based on his EAI Patterns book. Before we started discusssing the patterns in his book, we characterized messaging and talked about the various interaction styles. We also contrasted the messaging architectural style with an RPC based approach. We then took a look at the relationship to SOA, the role of contracts and the orchestration-vs-choreography discussion. We briefly discussed the nature of pattern languages before we then went through the different section in the book. There are six main sections: channel, message, routing, transfomation, endpoint as well as management and monitoring. We discussed the core patterns for each of these sections. This should give listeners a good high-level view of message-based systems. We concluded the discussion by looking at the critical importance of systems management and monitoring. Full Article
hp SE Radio Episode 308: Gregor Hohpe on It Architecture and IT Transformation By traffic.libsyn.com Published On :: Wed, 08 Nov 2017 17:44:12 +0000 Bryan Reinero talks with Gregor Hohpe about IT Transformation, the process by which organizations adapt and reorganize themselves in response to evolution and how the Enterprise Architect leads that transformation. Full Article
hp How to Read an RSS Feed with PHP Using SimplePie By www.rssboard.org Published On :: Mon, 26 Jun 2023 15:08:40 -0400 If you need to load an RSS feed with the PHP programming language, the open source library SimplePie greatly simplifies the process of pulling in items from a feed to present on a website, store in a database or do something else coooool with the data. There's a full installation guide for SimplePie but you can skip it with just three steps:Download SimplePie 1.5.Copy the file autoloader.php and the folder library to a folder that's accessible from your PHP code.Make note of this folder; you'll be using require_once() to load autoloader.php from that location.SimplePie has been designed to work the same regardless a feed's format. It supports RSS 2.0, RSS 1.0, Atom and the earlier versions of RSS. Additionally it can read feed elements from nine namespaces.Here's PHP code that loads feed items from the news site Techdirt and displays them in HTML:// load the SimplePie libraryrequire_once('/var/www/libraries/simplepie-1.5/autoloader.php');// load the feed$feed = new SimplePie();$feed->set_feed_url('https://www.techdirt.com/feed/');$feed->init();$feed->handle_content_type();// create the output$html_output = '';foreach ($feed->get_items() as $item) {ÃÂ ÃÂ $html_output .= '<p><a href="' . $item->get_link() . '">' . $item->get_title() . '</a></p>';ÃÂ ÃÂ $html_output .= $item->get_description();ÃÂ ÃÂ $html_output .= '<p>By ' . $item->get_author(0)->get_name() . ', ' . $item->get_date();}// display the outputECHO <<<END$html_outputEND;The API documentation for SimplePie_Item lists the functions that can extract data from each feed item. The versatility of the library is demonstrated by get_authors(), which can retrieve an item's authorship information whether it was in the RSS author element, Dublin Core creator, iTunes author, or Atom author.SimplePie supports caching so that a feed isn't downloaded every time code is executed. The addition of these lines turns on caching, specifies the location of a cache folder and sets the time to use a cached version to four hours (14,400 seconds):$feed->set_cache_location('/var/www/cache/');$feed->set_cache_duration(14400);$feed->enable_cache();SimplePie was created by RSS Advisory Board member Ryan Parman, Geoffrey Sneddon and Ryan McCue. The project is currently maintained on GitHub by Malcom Blaney. Full Article announcements
hp How HPP offers that added layer of food safety By www.packagingstrategies.com Published On :: Thu, 11 Sep 2014 00:00:00 -0400 Despite the nation’s economic turmoil and the ongoing food recalls and contamination problems, the world of high-pressure processing (HPP) has remained robust. Full Article
hp Securikett Unveils Security Label with Cut Indicator, to Showcase at FACHPACK By www.packagingstrategies.com Published On :: Mon, 23 Sep 2024 10:36:18 -0400 Securikett's new security labels offer an effective solution for detecting tamper attempts early, providing vital protection along the entire distribution chain and boosting customer trust in the process. Full Article
hp Shurtape HP 535 Hot Melt Packaging Tape By www.packagingstrategies.com Published On :: Tue, 09 Feb 2021 15:29:33 -0500 The heavy-duty packaging tape is designed for sealing high-value packages, 100% recycled corrugated cardboard. Full Article
hp WeighPack Announces New High Speed Continuous Motion Bagger By www.packagingstrategies.com Published On :: Wed, 30 Nov 2022 13:04:47 -0500 WeighPack has launched its newest vertical form, fill and seal machine for high-speed applications, the VF 1200 continuous motion bagger. Full Article
hp WeighPack Releases High Pressure Washdown Vertical Bagging Machine By www.packagingstrategies.com Published On :: Fri, 17 Feb 2023 14:27:04 -0500 The updated VF-1200 is more sanitary, allowing for high pressure washdown needs to be met. Full Article
hp CERVARIX human papillomavirus vaccine types 16 and 18 [recombinant, AS04 adjuvanted] suspension for injection pre-filled syringe (HPV Type 18 L1 Protein) By apps.tga.gov.au Published On :: Commercial Changes / Commercial viability Full Article
hp CERVARIX human papillomavirus vaccine types 16 and 18 [recombinant, AS04 adjuvanted] suspension for injection pre-filled syringe (HPV Type 16 L1 Protein) By apps.tga.gov.au Published On :: Commercial Changes / Commercial viability Full Article
hp HPR-40 DEMS by Hiden Analytical By nanotechweb.org Published On :: 2018-02-05T11:14:30Z Solutions for Dissolved Gas Analysis and Off-gas Analysis in Electrochemistry Full Article
hp HPR-20 TMS by Hiden Analytical By nanotechweb.org Published On :: 2018-02-05T11:51:32Z A specialist gas analysis system for fast event transient analysis Full Article
hp HPR-20 EGA by Hiden Analytical By nanotechweb.org Published On :: 2018-02-05T12:08:05Z A compact bench-top gas analysis system for evolved gas analysis in thermogravimetric mass spectrometry, TGA-MS Full Article
hp 5 Ways to Manage Multiple Versions of PHP By www.hongkiat.com Published On :: Mon, 28 Oct 2024 13:00:38 +0000 Managing multiple PHP versions is a common challenge when developing PHP applications, where applications often require different versions due to varying framework dependencies and compatibility requirements. While switching between PHP versions can be daunting, especially at the system level, several tools can streamline this process. In this article, we’ll explore effective solutions for managing multiple… The post 5 Ways to Manage Multiple Versions of PHP appeared first on Hongkiat. Full Article Coding PHP version control
hp What I hope to see at HP Amplify 2024 By www.hardwarezone.com.sg Published On :: Mon, 4 Mar 2024 15:28:27 +0800 This is my Wishlist of tech I'd like to see at HP Amplify later this week. #hp #hpamplify #ai #ar #vr Full Article From the Rumor Mill
hp HP Canada names Biase next president By channelbuzz.ca Published On :: Thu, 07 Nov 2024 14:40:40 +0000 Michelle Biase will return to HP Canada as its new president next month, while US-based executive Peter DiMarco takes over her role as interim general manager of distributor D&H Canada. Full Article Uncategorized
hp מתכנת /ת PHP מומחה לוורדפרס ואלמנטור By www.xplace.com Published On :: Tue, 12 Nov 2024 11:04:41 GMT אנחנו מחפשים מתכנת /ת. מתכנת /ת של קוד, של קסטום, של PHP ו CSS , מתכנת מעולה. אז אחרי תיאום הציפיות: מחפשים מתכנת /ת PHP תותח /ית לפרויקטים שוטפים של תחזוקה ופיתוח אתרי וורדפרס מתקדמים, מבוססי אלמנטור. אנחנו מחפשים מישהו /י עם ניסיון עשיר בעולמות האלו, עם עין לפרטים, זמינות גבוהה, ויכולת לתת פתרונות ברמה הגבוהה ביותר.דרישות שנשמח:לפחות 5 שנות ניסיון בפיתוח PHP, כולל ניסיון מעמיק בעבודה עם וורדפרס ואלמנטורשליטה מלאה בבניית קסטומיזציות ופתרונות ייחודיים באלמנטורניסיון בעבודה עם WooCommerce – ית... Full Article
hp CRUD Operations with Multiple Images in PHP By www.codexworld.com Published On :: Thu, 12 Oct 2023 06:43:33 +0000 CRUD operations are the most used functionality in the web application managed dynamically. The add, edit, and delete functionality helps to manage data with the database on the website. You can integrate CRUD operations with PHP to perform create (insert), read (select), update, and delete operations. If your web application works with images, CRUD functionality can be used to manage the images. In this tutorial, we will show you how to integrate CRUD operations with multiple images in PHP. In this example script, we will create product management system with images using PHP. The user can add, edit, update, and The post CRUD Operations with Multiple Images in PHP appeared first on CodexWorld. Full Article PHP CRUD Database MySQL
hp Generate QR Code Dynamically using PHP By www.codexworld.com Published On :: Mon, 06 Nov 2023 05:04:48 +0000 QR Code or Quick-Response code is machine-readable code consisting of squares arranged in square grid format. QR code is typically used to store information that can be readable by an imaging device (such as camera). QR codes may be used for various purposes, like displaying info to users, opening web page URLs, downloading mobile apps, adding contacts, etc. The user needs to scan QR code by device camera to view the information associated with the code. QR code or 2-dimensional barcode can be created dynamically. You can generate QR code using PHP. In this tutorial, we will show you how The post Generate QR Code Dynamically using PHP appeared first on CodexWorld. Full Article PHP Library QR QR Code
hp PayPal Advanced Checkout Card Payments Integration in PHP By www.codexworld.com Published On :: Fri, 12 Jan 2024 06:11:14 +0000 PayPal Advanced Checkout lets you accept credit and debit card payments directly from the website. You can set up custom credit card fields and add card payment form without any PayPal branding. The card input fields and payment form style can be customized as per the website design along with your business branding. The PayPal JavaScript SDK and REST API provide an easy way to integrate advanced checkout and credit card payment system in the web application. PayPal has 2 types of checkout systems, Standard and Advanced. If you want to embed PayPal branded payment component, integrate PayPal Standard Checkout The post PayPal Advanced Checkout Card Payments Integration in PHP appeared first on CodexWorld. Full Article PHP JavaScript PayPal
hp DataTables Column Filtering with Server-side Processing using PHP By www.codexworld.com Published On :: Thu, 07 Mar 2024 06:05:59 +0000 DataTables is a JavaScript library used to enhance the functionality and add advanced features to HTML tables. DataTables plugin converts a basic HTML table to an advanced feature-rich table instantly. There are various advanced features are available with DataTables, and server-side processing is one of them. DataTables server-side processing enables you to load a large number of data sets from the database. By setting the server-side option the search, pagination, and column sorting operations will be handled with the server-side script and database. Column filtering is a very useful option to help the user find specific records in a large The post DataTables Column Filtering with Server-side Processing using PHP appeared first on CodexWorld. Full Article PHP DataTables Filter MySQL Search
hp Upload Multiple Files with JavaScript using PHP By www.codexworld.com Published On :: Mon, 22 Apr 2024 05:59:05 +0000 Mostly the server-side scripting language such as PHP is used to upload file to the server. With this method, an HTML form is submitted to upload the selected file to the server. To provide a better user interface, we can use client-side technology such as jQuery Ajax to upload files without page refresh. Moreover, you can use simple JavaScript to upload files to the server. The JavaScript FormData interface provides an easy way to construct key/value pairs with form fields and send them to the server side using the XMLHttpRequest method. This technique can be used to upload files to The post Upload Multiple Files with JavaScript using PHP appeared first on CodexWorld. Full Article JavaScript File Upload PHP
hp Create Web Form with Drag and Drop File Upload using JavaScript and PHP By www.codexworld.com Published On :: Wed, 08 May 2024 11:59:02 +0000 The web form is a commonly used element in each website. In some cases, the user is allowed to select files with the input data so that the files are attached to the form data. Mostly the default HTML file input interface is used to select and upload files. But if you want to make web form UI more effective, drag and drop file upload feature can be used. The drag-and-drop feature provides an advanced file upload interface on the web page. The user can drag the files from the local drive and drop them into the DOM element. In The post Create Web Form with Drag and Drop File Upload using JavaScript and PHP appeared first on CodexWorld. Full Article JavaScript Drag&Drop File Upload Form PHP
hp PHP CRUD Operations with PostgreSQL Server By www.codexworld.com Published On :: Mon, 29 Jul 2024 08:19:17 +0000 CRUD (Create, Read, Update, and Delete) operations are used in the web application for the data manipulation in the database. There are four basic operations involved in the CRUD functionality that help to manage data with the database. We have already shared the tutorial to perform create (insert), read (select), update, and delete operations in PHP CRUD Operations with MySQL. In this tutorial, we will build PHP CRUD application with PostgreSQL server. PostgreSQL also known as Postgres is a relational database management system (RDBMS). The PostgreSQL database is open-source and free to use. We will connect with the PostgreSQL Server The post PHP CRUD Operations with PostgreSQL Server appeared first on CodexWorld. Full Article PHP CRUD PostgreSQL
hp Shopping Cart with PayPal Payment Gateway in PHP By www.codexworld.com Published On :: Wed, 16 Oct 2024 06:54:13 +0000 Shopping cart with payment gateway is a must-have functionality for an e-commerce website. The payment gateway allows the buyers to make payment online at the time of checkout. PayPal standard checkout is one of the easiest option to integrate payment gateway in the web application. A shopping cart helps users to select multiple products and PayPal allows users to checkout with credit/debit card payment. PHP shopping cart with PayPal makes e-commerce web applications more user-friendly and feature reach. In this tutorial, we will show you how to build shopping cart with payment gateway in PHP. This example shopping cart system The post Shopping Cart with PayPal Payment Gateway in PHP appeared first on CodexWorld. Full Article PHP Cart PayPal Shopping
hp Introducing iQue®'s 2nd Edition High-Throughput Cytometry Handbook: Fast. Simple. Discover the Future of Cell Analysis! By www.the-scientist.com Published On :: Tue, 29 Oct 2024 19:19:58 GMT This handbook is designed to empower both new and seasoned flow cytometry users who are curious about the unique capabilities of HTS cytometry. Full Article The Scientist The Marketplace
hp Curtain rises at Shakespeare’s Birthplace By thebirminghampress.com Published On :: Tue, 25 Jul 2023 22:20:09 +0000 Live actors enter the stage for the summer. Full Article Attractions Books Education Exhibitions Family Local history All the World's a Stage Events in History First Folio Shakespeare Shakespeare’s Birthplace
hp UNIX BSD Linux SunOS HP-UX & Perl Man Pages: man Page By manpage.me Published On :: 2024-11-13T08:48:21+00:00 man pages for UNIX, BSD, Linux, SunOS, HP-UX, AIX, Minux, Ultrix, Plan9, Darwin, XFree86, & Perl Man & Info Pages, plus Application manuals Full Article
hp SHPAMEE Project Updated By www.cybertopcops.com Published On :: Tue, 12 Aug 2008 16:38:29 GMT The SHPAMEE Project has been updated with new examples. For those who haven't noticed yet, we have a separate feed for updates to this project: http://feeds.feedburner.com/shpamee Full Article
hp Problems With SHPAMEE Feed By www.cybertopcops.com Published On :: Thu, 11 Oct 2012 19:24:39 GMT It seems like the SHPAMEE feed was not updating correctly. This issue has now been resolved. Please let us know if you are still experiencing problems with the SHPAMEE feed. Full Article
hp HPWC: Hot Peppers Netball To Return By bernews.com Published On :: Thu, 03 Oct 2024 19:29:42 +0000 Hamilton Parish Workmen’s Club announced the return of the Hot Peppers Netball Team, with registration open as of October 2, 2024, and over 20 players already expressing interest. A spokesperson said, “Hamilton Parish Workmen’s Club [HPWC or the Club] is thrilled to announce the return of the Hot Peppers Netball Team. Registration was opened on […] Full Article All Sports #Netball
hp Netball: Storm Secure Victory Against HPWC By bernews.com Published On :: Thu, 07 Nov 2024 18:00:52 +0000 The Storm secured a decisive victory over HPWC in a netball game, with a final score of 78-5. The Storm took an early lead and maintained control throughout the match. Nabilah Nasir led the scoring for the Storm with 37 goals, followed by Rihanna Reddicks with 21 and Zukiyah Durham with 20. Kiarra Francis and […] Full Article All Sports #GoodNews #Netball
hp Equestrian: BHPA Winter Jumper Festival Results By bernews.com Published On :: Tue, 13 Feb 2024 11:49:00 +0000 The BHPA Winter Jumper Festival was held at the National Equestrian Center on Vesey Street. Class 1 – 0.65 m 1st Moxie & Paige Lindo 2nd Mr. Wizard & Charlette Pantry 3rd Wild Things Island Adventure & Logan Flood 4th Kool Blu & Tonae Tavares 5th Puff the Magic Dragon & Bella Rodrigues Class 2 […] Full Article All Sports #Equestrian
hp HPT Treasures: Practical Situational Awareness By www.bill-wilson.net Published On :: Fri, 24 Sep 2021 00:50:23 +0000 I posted about Situational Awareness at HPT Treasures today. What I didn't say in that post was that I've experimented with a few different methods... Full Article Human Performance human performance tools situational awareness
hp THPS Rules! By radar.spacebar.org Published On :: Sat, 30 Mar 2024 18:05:25 -0400 Oof, so busy! I finished up my paper(s) for SIGBOVIK and submitted them. Phew. I'll post 'em here after the embargo ends. I think the papers may be the canonical form of this particular project, but I'm starting on a visual version, which will probably become a video some time this month. (First up: I need to prep a live version for Unnecessary Detail, as mentioned in the previous post.) But right now I'm also on the West coast (in a car traveling from Los Angeles to San Diego) for a short family trip which was cut even shorter by the Spirit of Bad Aircraft Management of Spirit Airlines. Immediately upon arriving at the hotel I looked out our window and saw a little park and thought, "it would be fun to skateboard in that park," (I am not a skateboarder) and then, "did I already skateboard in that park 20 years ago in Tony Hawk's Pro Skater?" and I looked it up and yes, the Los Angeles level in THPS3 is based on that very park. I thought I wasn't going to be able to make the SIGBOVIK live event at all because of this trip, but it looks like I will be back and might try to make it, depending partly on whether I can get any material together for a talk in time. I spent most of my free time in March on hacking and writing for this silly paper, but sometimes the brain needs a break, and I continued with Teardown. I think this game is great. It is impressive technically and graphically. The sandbox is fun; I had a great time painstakingly disassembling an enormous blast furnace until the framerate became intolerable. (The way the physics works, the entire blast furnace can be held up by the connection of a single voxel. This is obviously totally unrealistic but it is pretty fun to try to blowtorch around an entire building and then try to hunt down why it is still standing.) But I was also impressed with how they managed to make the missions compelling too. There are a couple of ergonomic annoyances (like: There are limitations on what keys can be rebound to what, so I had to play with a controller. And the quick-save is great, but given that you might spend an hour setting up a heist in a level, it would be nice if you could make an in-level save that was a little less quick, just in case you accidentally stayed up to 2am). Getting close to the end of that one. I also played through Gunlocked, which was a good small Roguelike shoot-em-up. The powerups were really well done; it just could've used a bit more variety in the bad guys. OK, getting a bit carsick here so I'm going to put the laptop away. See you soon. Full Article
hp Webinar: nieuw HPV-screeningsbeleid en -vaccinatie - 19 november 2024 - Medi-Sfeer By news.google.com Published On :: Wed, 13 Nov 2024 08:08:57 GMT Webinar: nieuw HPV-screeningsbeleid en -vaccinatie - 19 november 2024 Medi-Sfeer Full Article
hp An all-new 650 hp Cadillac, Hertz is selling off more EVs, and something weird at Tesla By biztoc.com Published On :: Wed, 13 Nov 2024 07:52:52 GMT On today’s exciting episode of Quick Charge, we’ve got the Vistiq! It’s all-new, three row SUV from Cadillac that packs 650 hp and can go from 0-60 mph in under 3.5 seconds, a serious word from Rivian, and something fishy at Tesla. We’ve also got word that Hertz is selling off even more of its… Full Article
hp HPE Issues Critical Security Patches for Aruba Access Point Vulnerabilities By thehackernews.com Published On :: Mon, 11 Nov 2024 15:27:00 +0530 Hewlett Packard Enterprise (HPE) has released security updates to address multiple vulnerabilities impacting Aruba Networking Access Point products, including two critical bugs that could result in unauthenticated command execution. The flaws affect Access Points running Instant AOS-8 and AOS-10 - AOS-10.4.x.x: 10.4.1.4 and below Instant AOS-8.12.x.x: 8.12.0.2 and below Instant AOS-8.10.x.x: Full Article
hp SHPE General Meetings By www.princeton.edu Published On :: Wed, 27 Nov 2024 18:00:00 -0500 The Society of Hispanic Professional Engineers meets weekly on Wednesdays in the Yeh PDR to come together, socialize, and envision the future of the club together. Full Article
hp SHPE General Meetings By www.princeton.edu Published On :: Wed, 20 Nov 2024 18:00:00 -0500 The Society of Hispanic Professional Engineers meets weekly on Wednesdays in the Yeh PDR to come together, socialize, and envision the future of the club together. Full Article
hp The perfect ski holiday in St Moritz, the birthplace of winter sports By www.telegraph.co.uk Published On :: Mon, 14 Oct 2024 16:00:00 GMT Full Article structure:travel/ski structure:travel structure:travel/ski/resort-guides topics:places/st-moritz topics:places/switzerland storytype:standard structure:eg-general structure:better-life/better-life-evergreen structure:better-life/better-life-guide topics:places/europe structure:travel/destination-guides-100
hp Add a WordPress Admin User Account via PHP By www.tipsandtricks-hq.com Published On :: Sun, 18 Oct 2020 06:09:52 +0000 Sometimes our WordPress plugin users need to create an Admin user account for their sites. In this tutorial I will share a small PHP code with you that can be used to create a WordPress Administrator user to your site. Alternatively, you can also create a WordPress admin user via MySQL. You will need to […] The post Add a WordPress Admin User Account via PHP appeared first on Tips and Tricks HQ. Full Article Web Development Wordpress creating WordPress plugin Site admin tips Tech Tips Troubleshooting
hp Development and validation of a high-throughput whole cell assay to investigate Staphylococcus aureus adhesion to host ligands [Microbiology] By www.jbc.org Published On :: 2020-12-04T00:06:05-08:00 Staphylococcus aureus adhesion to the host's skin and mucosae enables asymptomatic colonization and the establishment of infection. This process is facilitated by cell wall-anchored adhesins that bind to host ligands. Therapeutics targeting this process could provide significant clinical benefits; however, the development of anti-adhesives requires an in-depth knowledge of adhesion-associated factors and an assay amenable to high-throughput applications. Here, we describe the development of a sensitive and robust whole cell assay to enable the large-scale profiling of S. aureus adhesion to host ligands. To validate the assay, and to gain insight into cellular factors contributing to adhesion, we profiled a sequence-defined S. aureus transposon mutant library, identifying mutants with attenuated adhesion to human-derived fibronectin, keratin, and fibrinogen. Our screening approach was validated by the identification of known adhesion-related proteins, such as the housekeeping sortase responsible for covalently linking adhesins to the cell wall. In addition, we also identified genetic loci that could represent undescribed anti-adhesive targets. To compare and contrast the genetic requirements of adhesion to each host ligand, we generated a S. aureus Genetic Adhesion Network, which identified a core gene set involved in adhesion to all three host ligands, and unique genetic signatures. In summary, this assay will enable high-throughput chemical screens to identify anti-adhesives and our findings provide insight into the target space of such an approach. Full Article
hp Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics By www.jlr.org Published On :: 2008-05-01 Vitali MatyashMay 1, 2008; 49:1137-1146Methods Full Article