The computer program can identify canine versus human feces based on DNA sequences in samples

Items recovered include fossils, paintings, ancient coins, ceramics and jewelry

The app features a database of some two million artworks housed at more than 120 venues

Graffiti artists and muralists are sending messages of hope and despair with coronavirus public art

The projects are helping Australia's drought-stricken rural towns find new life as outdoor art galleries

The first major exhibition of its kind, "Hearts of Our People," boasts 82 pieces from 115 Native women across North America

Highway 8, otherwise known as the Dempster Highway, will be closed to all traffic at 5 p.m. on Friday, according to the government of the Northwest Territories.

The following is a guest post by Katherine Blood, Curator of Fine Prints, Prints & Photographs Division. As the Library of Congress marks its 220th year of serving the nation, the publication of a new guide tells two stories: how staff have for decades worked with art professionals to build the collections and how by […]

Successful modification of the bacterial artificial chromosome (BAC) after two-step BAC engineering is confirmed in two separate polymerase chain reactions (PCRs). The first reaction (5' co-integrate PCR) uses a forward 5' co-integrate primer (a sequence located upstream of the 5' end of the A-box) and a reverse 3' primer on the vector (175PA+50AT) or within the reporter sequence or mutated region as appropriate. The second reaction (3' co-integrate PCR) uses a forward 5' primer on the recA gene (RecA1300S) and a reverse 3' co-integrate primer (a sequence located downstream from the 3' end of the B-box). Those colonies shown to be positive in PCR analysis are further tested for sensitivity to UV light. After the resolution, colonies that have lost the excised recombination vector including sacB and recA genes become UV light sensitive.

Bacterial artificial chromosome (BAC) clones are rendered electrocompetent and transformed with the recombinant shuttle vector, pLD53SCAB/AB-box. Cointegrates are selected by growth on chloramphenicol and ampicillin to ensure recombination of the shuttle vector into the BAC.

Plasmid DNA is prepared from the recombinant shuttle vector pLD53.SCAB/A-B created by cloning of the A and B homology arms for two-step bacterial artificial chromosome (BAC) engineering. To confirm that the A-box and B-box arms have been successfully incorporated into pLD53.SCAB, the pattern of enzyme digestion of the modified plasmid is compared with that of the unmodified pLD53.SCAB. Once the shuttle vector is shown to carry the proper sequences, it is ready for transfer into the BAC host.

This protocol describes the preparation of the shuttle vector before its introduction into bacterial artificial chromosome (BAC) host cells for BAC two-step engineering. The homology arm sequences, prepared previously, are introduced by ligation into the digested shuttle vector DNA to provide sites for recombination within the BAC clone. Crude lysates of individual bacterial transformants serve as templates in polymerase chain reaction (PCR) analysis to confirm the presence of the homology arms in the recombinant shuttle vector.

The 700-bp A homology arm (A-box) and the 700-bp B homology arm (B-box) are amplified by polymerase chain reaction (PCR) using purified bacterial artificial chromosome (BAC) DNA as template for two-step BAC engineering. The resulting A-box PCR product contains an AscI site at its 5' end (the 5' primer incorporates an AscI site, and the 3' primer does not incorporate any restriction sites). The B-box PCR product contains an XmaI site at its 3' end (the 5' primer does not incorporate any restriction sites, and the 3' primer incorporates an XmaI site). The amplification products are then digested with the appropriate restriction endonucleases to render them suitable for cloning into the shuttle vector.

In two-step bacterial artificial chromosome (BAC) engineering, a single plasmid is introduced into the BAC-carrying cell lines. The shuttle vector pLD53.SCAB (or pLD53.SCAEB) carries the recA gene and the R6K origin, which requires the protein to replicate. PIR2 cells, expressing , are typically used for the amplification of the vector and maintain about 15 copies/cell of the donor vector, which is relatively stable in this host.

Last month, the National Arts Centre launched #CanadaPerforms, an initiative that pays musicians and writers to perform from their living rooms. Poet Zoey Roy gave an intimate performance from her living room in Kingston. 

Toronto officials reported 180 new cases of COVID-19 on Thursday, a figure that represents a dip when compared to the more than 200 cases announced daily this week. 

As truckers move vital supplies such as food, hand sanitizer and ventilators during the COVID-19 pandemic, they're pleading with governments to allow more truck stops to reopen so they can get meals, showers and rest. Social media groups and individual businesses are doing their part to thank truckers.

Team Homan, Canada’s 2018 Olympic women’s curling team and the 2017 World Women’s Curling Champions have announced that the team has parted ways with lead Lisa Weagle. 

Hamilton Conservation Authority is re-opening the Hamilton-to-Branford Rail Trail, while the city announced the reopening of the Hamilton Waterfront Trail, between Confederation Park and the Burlington Lift Bridge.

CNA Staff, May 5, 2020 / 10:00 am (CNA).- Poland’s bishops have intervened in a debate about whether presidential elections scheduled for May 10 should go ahead despite a nationwide lockdown.

A statement from the permanent council of the Polish bishops’ conference April 27 urged politicians to work together to ensure that the election would be regarded as legitimate by all sides. 

It said: “We appeal to the consciences of those responsible for the common good of our homeland, both those in power and the opposition, to work out a common position on the presidential elections in this extraordinary situation.” 

Poland’s ruling coalition, led by the Law and Justice (PiS) party, has rejected calls to postpone the election, due to take place this Sunday. 

The state began introducing lockdown measures March 10, which it is now starting to lift. Poland, which has a population of almost 38 million, had 14,242 documented coronavirus cases and 700 deaths as of May 5, according to Johns Hopkins University Coronavirus Resource Center.

The Polish Senate began debating legislation May 5 that would allow the election to be held by postal vote, rather than at polling stations, due to the pandemic. 

The Sejm, the lower chamber of the Polish parliament, will have the final say on the legislation. 

The bishops called on lawmakers to resolve the issue while upholding the principles of Poland’s constitution. They emphasized that they were not seeking to engage in “purely political disputes over the form or timing of election, let alone to advocate this or that solution.”

The bishops’ permanent council said: “We encourage dialogue between the parties to seek solutions that would not raise legal doubts and suspicion, not only of a violation of the current constitutional order but also of the principles of free and fair elections adopted in a democratic society.”

“We ask that, guided by the best will, they would seek in their actions the common good, which today is expressed both by the life, health and social existence of Poles, as well as broad social trust in the electoral procedures of a democratic state jointly developed over the years.”

The bishops continued: “In this difficult situation that we are experiencing, we should take care to cultivate a mature democracy, protect the nation of laws, building -- despite differences -- a culture of solidarity, also in the political sphere.”

If parliament approves the postal vote, the government could delay the vote to either May 17 or May 23 to allow more preparation time, according to Reuters. 

Opinion polls suggest the incumbent President Andrzej Duda, a PiS ally, would be re-elected by a significant margin if the vote were held soon. 

Bishops’ conference president Archbishop Stanisław Gądecki entrusted Poland to the Most Sacred Heart of Jesus and to Our Lady, Queen of Poland, at Jasna Góra Monastery in Częstochowa May 3.

OM Ireland's biggest outreach of the year teaches participants to commit their ways to Him and trust Him to act.

Thirteen participants from all over the world spent a week getting to know and supporting the refugee work in Linz. Six share about their experiences.

Shetland paella puts a Scottish spin on this seafood favourite. "This was the last dish I cooked on the trip and it really summed up the amazing produce they have in this part of the world, featuring both local fish and shellfish," says chef James Martin, recalling the adventures he had making his Islands To Highlands series.

Dear Editor,Rumours have more potential to cause chaos now more than ever before. Previously, misconceptions and mischaracterisations took longer to infect the minds of a society. Maybe that left us unprepared.

Prudence Wade chats to self-confessed 'skincare junkie' Dominic Skinner, one of MAC's top make-up artists.

Big Zuu is headed back out on tour - but not as we know it.

WITH the usual proviso that nothing, but nothing, in sport is in any way important when human lives are at stake due to coronavirus, nevertheless I do think it is time for some realism to surface in rugby – and other sports I could name.

The great North-South divide in rugby was never more in evidence than when the votes were counted for the chairmanship of World Rugby last week. Sir Bill Beaumont stayed in the job, beating Agustin Pichot by 28 votes to 23, but wow, what an outcome in terms of who actually supported the former England and British Lions captain.

PLANS for league reconstruction have been scrapped after Ladbrokes Premiership clubs indicated there isn't enough support for the proposed changes at this time.

Music

For three decades now, the artist and playwright John Byrne has been sitting regularly for photographer David Eustace, the Glasgow-born photographer who left school at 16 and joined first the navy and then the prison service before settling on a career behind a camera.

By John-Paul Holden

OM Japan and an outreach team encourage and help people who suffered from the tsunami in Ishinomaki and the area around Minami Sanriku.

A pastor and OM couple start a new church in Tonami, Japan, a rural town of 50,000.

Estimates of youth participation in strangulation activity, commonly referred to as the "choking game," range from 5% to 11%. Previous studies have documented correlations between youth choking game participation and health risks such as substance use and mental health issues.

Among Oregon eighth-graders surveyed, >6% had ever participated in the choking game. Participation was linked to poor nutrition and gambling among females, exposure to violence among males, and sexual activity and substance use among both genders. (Read the full article)

Pediatric osteoarticular infections can be treated with successful microbiologic and clinical outcomes with a transition from parenteral to oral therapy. The best way to determine the timing of this transition is neither well studied nor standardized.

A total of 193 (99.5%) of 194 pediatric patients with acute bacterial osteoarticular infections were successfully transitioned to oral therapy, determined by using a combination of clinical findings and C-reactive protein levels, representing the largest single-center data set analyzed. (Read the full article)

Understanding human disease genomics requires large population-based studies. There is lack of standardization, as well as social and ethical concerns surrounding the consent process for pediatric participation in a biorepository.

The study identifies specific barriers to pediatric participation in biorepositories relative to adults, and proposes strategies to improve ethical and responsible participation of pediatric-aged patients in large-scale genomics and biorepository-driven research without significantly increasing research burden for affected families. (Read the full article)

There is evidence that Kingella kingae, the major bacterial cause of osteoarticular infection in children <4 years of age, first colonizes the oropharynx before penetrating the bloodstream and invading distant organs. Diagnosis remains challenging because clinical findings at admission may be normal.

Our study demonstrated for the first time that a simple technique of detecting of K kingae DNA in the oropharynx can provide strong evidence that this microorganism is responsible for the OAI, or even stronger evidence that it is not. (Read the full article)

The Supplemental Nutrition Assistance Program (SNAP) is the largest federal nutrition assistance program. Studies among adults suggest that SNAP participation may be associated with suboptimal diets. Few studies have extensively examined these associations among children.

SNAP participation was not associated with childhood obesity. SNAP children consumed diets poorer in some aspects than nonparticipants, but intake of some micronutrients was higher. The diets of both groups of low-income children were far from meeting dietary guidelines. (Read the full article)

Forgoing artificial nutrition and hydration in children at the end of life is an acceptable practice under some circumstances. However, there is a paucity of pediatric evidence to guide health care providers’ and parents’ decision-making around this practice.

This study describes experiences of parents whose children died after forgoing artificial nutrition and hydration. All parents were satisfied with their decision and believed their child’s death was peaceful. This study adds to the limited evidence to guide clinical practice. (Read the full article)

Recent studies have shown that participating in the Supplemental Nutrition Assistance Program (SNAP) is associated with improved household food security. With the exception of 1 descriptive analysis, studies have not examined how SNAP affects children’s food security.

This article estimates the association between SNAP and children’s food security using the largest, most rigorous national study of food security to date. Given current proposals to reduce program size, this study underscores SNAP’s importance in affecting children’s well-being. (Read the full article)

In-person peer counseling to pregnant and new mothers has been shown to improve breastfeeding modestly in three US RCTs. But this level of support for WIC is unlikely to be scaled up nationally in the current fiscal environment.

We randomly assigned WIC clients to a telephone peer counseling program relative to standard WIC support for breastfeeding. Nonexclusive breastfeeding among Spanish-speakers increased at 1, 3, and 6 months, but the program had much less of an effect on English-speaking clients. (Read the full article)

In 2003, the US Food and Drug Administration issued warnings about hyperglycemia and diabetes with second-generation antipsychotics (SGAs). Since 2004, hyperglycemic and diabetes risk with SGAs has been stated in product labels, and published guidelines have recommended baseline metabolic screening.

Between 2006 and 2011, 11% of children 2 to 18 years starting an SGA had baseline glucose assessed. Youth at risk for diabetes may not be identified. Further, lack of screening impedes determining the contribution of SGAs to hyperglycemia. (Read the full article)

Preparticipation physical evaluations (PPEs) are considered necessary for a high standard of care for US scholastic athletes. However, important questions remain regarding consistency of implementation and content of cardiovascular screening practices among states.

Our results show that PPE policies are variable among US states, and adoption of current PPE-4 best practices is slow, demonstrating the need for nationwide PPE standardization. (Read the full article)

Head Start, a federally funded preschool program for low-income US children, has been reported to have beneficial effects on developmental outcomes. The association of Head Start participation with changes in children’s BMI has not been examined.

Preschool-aged children with an unhealthy weight status who participated in Head Start had a significantly healthier BMI by kindergarten entry age than comparison children in a primary care health system (both those receiving and those not receiving Medicaid). (Read the full article)

This article, published ahead of print on 28 July 2008, has been withdrawn by the authors. Although moderate synergy between P2-RANTES and C peptides can be observed with high statistical significance in cell fusion assays, this synergy was not able to be verified in HIV viral assays. The authors regret the overstatement of synergy and will revise the paper for publication at a later date.