ng Innovative Financing for Humanitarian Energy Interventions By www.chathamhouse.org Published On :: Thu, 28 Feb 2019 03:42:23 +0000 Innovative Financing for Humanitarian Energy Interventions Research paper sysadmin 28 February 2019 This paper explores the increase in resources and funding needed to improve the access of displaced people to modern and sustainable energy services. — SOLARKIOSK stall in Talek, Kenya on 17 May 2017. Photo: Getty Images Summary In settings that host displaced and refugee communities, energy can act as an enabler for improved healthcare, education and access to clean water. More efficient sources of energy can also save money that can be reinvested in life-saving interventions. A range of challenges exist that inhibit the uptake and effective management of cleaner energy solutions in displacement settings. These are magnified by a lack of available and appropriate funding. The current funding gap is significant. In many cases, involving the private sector (both enterprises and investors) is viewed as a way to accelerate delivery of sustainable energy solutions, leverage additional capital, efficiency and expertise, and adopt more sustainable and market-based approaches. Displacement settings are an extreme example of complex and unpredictable operating environments. Traditional approaches to the financing of energy access will not be supported by the risk/return characteristics of this market opportunity, so alternative structures are needed. Such structures can include mechanisms such as grants, guarantees, ‘results-based financing’ and ‘impact bonds’. These blended financial instruments should aim to leverage first losses – whereby, in the case of default, the first loss is taken by the ‘impact-first’ investors, or guarantors, thereby fully or partially protecting ‘finance-first’ investors. Given the specific constraints of displacement settings, any financing mechanisms at present are likely to fall between the categories of providing ‘more efficient aid’ and ‘more efficient aid through markets’. They are likely to constitute a transitional step from grant-making towards the use of commercial investment vehicles. While a number of financial mechanisms could be applied to attract private-sector engagement, most remain theoretical, with few being implemented extensively or at scale. Where such financial mechanisms have already been used, access to relevant data is poor, especially in circumstances where the desired outcomes were not achieved. The Moving Energy Initiative (MEI) completed feasibility work into the concept of an energy humanitarian fund and found that, while a need for this type of facility has emerged, it sits in a difficult position between energy access, climate and humanitarian funding sources. Key donors are needed to drive forward innovative financing vehicles and further testing of these mechanisms, in order to generate market data and evidence for further iterations and additional investments. 2019-02-2019-InnovativeFinancingforHumanitarianEnergy (PDF) Full Article
ng Adopting a Market-based Approach to Boost Energy Access in Displaced Contexts By www.chathamhouse.org Published On :: Mon, 25 Mar 2019 10:18:12 +0000 Adopting a Market-based Approach to Boost Energy Access in Displaced Contexts Research paper sysadmin 25 March 2019 This paper evaluates the market-based approaches adopted in the MEI projects in Kenya and Burkina Faso. It articulates how such commercial strategies can be applied to the delivery of energy in displacement settings and compares this to real world examples. — A shop selling fabric and electronics inside the Dagahaley Camp, one of five camps that make up Dadaab, the world’s largest and oldest camp for refugees. Photo: Michelle Shephard/Toronto Star via Getty Images. Development of long-term energy solutions in displacement settings tends to be perceived as investment that falls outside the remit of emergency responses. In addition, when emergency energy supply measures are implemented they often result in expensive, unreliable and unhealthy energy provision for those in protracted or recurrent crises. There is widespread agreement among humanitarian and development experts that an effective refugee response should include long-term development solutions as well as emergency relief. The energy access imperative is more pronounced when considering the need for effective energy distribution in practically all camp activities and basic necessities: pumping and treatment of clean water; heating and cooling for food storage and cooking; energy for livelihood activities; and provision of light for schooling, hospitals and the prevention of violence against women and children. Minor shifts in household energy use to basic solar lighting options and non-wood fuels would save $303 million annually on refugee fuel costs. Within refugee contexts in Kenya and Burkina Faso, the MEI sought to examine opportunities to use market interventions, rather than in-kind distributions, to improve clean energy access over the long-term and test the delivery of market-based approaches. 2019-03-25-MEIWhitehouse (PDF) Full Article
ng Moving Energy Initiative Learning Briefs By www.chathamhouse.org Published On :: Fri, 29 Mar 2019 15:22:51 +0000 Moving Energy Initiative Learning Briefs Research paper sysadmin 29 March 2019 Drawing on experiences from Phase II of the MEI in Burkina Faso, Kenya and Jordan, these learning briefs highlight MEI’s approach to innovation, engagement with the private-sector and host communities, and gender-sensitive energy projects. The four learning papers are intended for practitioners and policymakers working in the humanitarian sector and host-country governments. — A shelf of energy appliances in a shop in Kakuma Town, Kenya. Photo credit: Gabriela Flores Findings from Phase I of the Moving Energy Initiative (MEI) in 2015, published in the Chatham House research paper Heat, Light and Power for Refugees: Saving Lives, Reducing Costs, highlight the negative impacts of limited sustainable energy provision on the security of displaced populations. The paper also identified some of the challenges for energy programmes in this sector, such as the lack of robust data on energy access and the priorities of refugee populations. In Phase II of the MEI, Practical Action led detailed research into the energy needs of refugees in Burkina Faso and Kenya. Chatham House analysed data on global refugee energy use in displacement contexts and produced an interactive map. Energy 4 Impact explored sustainable funding options, private-sector contract models and non-wood cooking concessions. The market development and low-carbon energy initiatives in Burkina Faso, Jordan and Kenya were managed by Practical Action and Energy 4 Impact, with the support of local partners. These partners represented the MEI at multiple conferences and events to share findings and advocate for the inclusion of displaced people in the sustainable energy agenda. Drawing on experiences from Phase II of the MEI in Burkina Faso, Kenya and Jordan, these learning briefs highlight MEI’s approach to innovation, engagement with the private-sector and host communities, and gender-sensitive energy projects. The four learning papers are intended for practitioners and policymakers working in the humanitarian sector and host-country governments. 2019-03-29-PrivateSectorEnergy (PDF) 2019-03-2019-EffectiveEngagement (PDF) 2019-03-29-PoweringInnovation (PDF) 2019-03-29-EnergyProgrammeVAWG (PDF) Full Article
ng Infrastructure Management Contracts: Improving Energy Asset Management in Displacement Settings By www.chathamhouse.org Published On :: Wed, 17 Apr 2019 15:09:12 +0000 Infrastructure Management Contracts: Improving Energy Asset Management in Displacement Settings Research paper sysadmin 17 April 2019 This paper highlights a number of options for managing electricity infrastructure in refugee camps and outlines the challenges, opportunities and operational implications associated with them. It takes the Kalobeyei settlement in Kenya as a case study. — A solar array installed for International Rescue Committee health clinics in Kakuma refugee camp, Kenya. Photo: Kube Energy Building and maintaining electricity infrastructure to power offices, businesses, households and other operations in displacement settings is difficult. It is especially challenging for the Office of the United Nations High Commissioner for Refugees (UNHCR) and its partner agencies, because supplying electricity is not their core business. Private-sector companies exist that are willing and able to develop infrastructure management contracts to provide energy as a service in displacement settings. However, institutional barriers within humanitarian agencies persist, with short budgeting cycles in particular preventing humanitarian agencies from entering into the sorts of long-term service agreements required by the private sector. A number of options exist to leverage the expertise of the private sector through ‘public–private partnership’ (P3) structures. Such mechanisms can promote more efficient management of infrastructure by drawing on private-sector experience and expertise, incentivizing appropriate risk-sharing and providing options to leverage private capital in project development. Field work from the Kalobeyei settlement in Kenya suggests that a solar/diesel hybrid mini-grid solution was the most economical option to power camp services and infrastructure there. Compared to distributed diesel generation, the annual savings in operating costs were estimated at $49,880, with the additional investment paid back within 3.6 years. Humanitarian agencies need to be willing to change their policies to enable long-term service agreements. Alternatively (or, more likely, in conjunction with this option), financial mechanisms such as partial risk guarantees need to be developed to offset some of the risks. This change will need high-level support from donors and humanitarian agencies. Once the first infrastructure management contracts can be signed and tested in displacement locations (through the use of donor funding or otherwise) and associated data collected, it will ease the way for future investments in these types of projects. 2019-04-17-MEI-Infrastructure Management Contracts (PDF) Full Article
ng Delivering Sustainable Food and Land Use Systems: The Role of International Trade By www.chathamhouse.org Published On :: Fri, 20 Sep 2019 09:03:21 +0000 Delivering Sustainable Food and Land Use Systems: The Role of International Trade Research paper sysadmin 20 September 2019 This paper explores a set of core trade-related issues affecting the food and land use system, and proposes constructive ways forward in reconfiguring the global trading system towards delivering a more sustainable and healthy diet for all. — Aerial view of containers sitting stacked at Qingdao Port in the Shandong province of China. Photo by Han Jiajun/Visual China Group via Getty Images. Meeting future global food security requirements is not just about quantity; it is also about meeting growing needs in a way that safeguards human as well as planetary health. But national priorities and policies often remain out of sync with aspirations for more sustainable and healthy food systems. International trade and trade policies play an ambiguous role in the current food system. With 80 per cent of the world’s population depending on imports to meet at least part of their food and nutritional requirements, trade has a unique function in offsetting imbalances between supply and demand. However, in the absence of effective regulatory frameworks or pricing frameworks that internalize environmental, social or health costs, trade can exacerbate and globalize challenges associated with food production and land use trends such as deforestation, land degradation, greenhouse gas emissions, biodiversity loss and the shift to unhealthy diets. Over the last two decades, trade in agricultural products (excluding intra-EU flows) has more than tripled in value, to reach $1.33 trillion. The geography of global food trade flows has also shifted, primarily towards South–South trade, which now accounts for roughly a quarter of total agricultural trade flows. The nature of global trade has changed drastically, with traditional exports such as wheat and coffee growing slowly at around 2 per cent per year, while products such as palm oil, fruit juice, soft drinks and other processed products have grown at 8 per cent or more annually. This overall increase in trade in agricultural products raises questions about the growing utilization of resources, such as water or soil nutrients, that are embedded in those products through production and processing. Trade itself also causes negative environmental impacts, starting with greenhouse gas emissions associated with transport and storage. If the environmental cost associated with production and trade is not reflected in the final price of goods, trade may accelerate the depletion of resources or their unsustainable use. It is critical to ensure that trade policy options pursued by producing and consuming countries alike will support a transition to more sustainable and healthier food and land use systems. The first step in addressing trade-related food systems challenges must involve rebuilding trust among policy actors. There is a need for new spaces for informal dialogue among actors, and ‘soft’ governance mechanisms that can help rebuild consensus on the best ways forward. Meeting these challenges also requires an appreciation of the complex interactions between sectoral policies (e.g. on water, land, food, etc.) and their multiple interfaces with trade policies. Conditioning the use of subsidies on their sustainability and/or health impacts encourages the delivery of essential public goods in ways that are consistent with sustainability and health goals. A first step therefore is the removal of perverse incentives (e.g. subsidies encouraging the overuse of fertilizers or pesticides or the overproduction of certain commodities, as well as certain biofuels subsidies) and replacing them with market-correcting subsidies. Trade facilitation measures for fruits and vegetables that are aimed at easing transit at the border, by cutting unnecessary bureaucracy and reducing waiting times, can improve their availability, reduce costs and improve food quality and safety for consumers. Similarly, measures aimed at improving sustainable cold storage and upgrading value chains can support better diets and consumption by increasing the availability of fresh produce on markets, especially in developing countries. A global food stamps programme developed through the G20 and facilitated by the UN’s food agencies could address purchasing power imbalances and tackle malnutrition in developing countries. If carefully designed, such ‘safety net’ schemes can not only contribute to improving calorific intakes but also help deliver more balanced and healthier diets. Careful attention must be given to how such a scheme would work in practice, building on experience to date with similar initiatives. Integrating the notion of sustainable food and inputs trade in the post-2020 global biodiversity framework can help to deliver more sustainable and healthier food and land use systems. This could be achieved by likeminded countries introducing a set of goals or targets aimed at mitigating the role of trade in placing indirect pressure on biodiversity, and to encourage trade in biodiversity-based products including natural ingredients produced ethically and following sustainability principles and criteria. An SDG-oriented agenda for agricultural trade is needed. It could be formed by countries seeking to remove perverse incentives, guaranteeing a safe harbour for market-correcting measures, clarifying existing rules and establishing plurilateral negotiations among subsets of the WTO membership, or sectoral approaches, to address specific challenges. Greenhouse gas emissions resulting from trade need to be addressed. Governments could seek to achieve this through ensuring the carbon neutrality of existing and new trade deals, either by connecting carbon markets among contracting parties or by developing joint initiatives to tax international maritime and air transport emissions. 2019-10-14-HoffmanCentreTradeandFoodSystems (PDF) Full Article
ng Online Disinformation and Political Discourse: Applying a Human Rights Framework By www.chathamhouse.org Published On :: Tue, 05 Nov 2019 11:03:02 +0000 Online Disinformation and Political Discourse: Applying a Human Rights Framework Research paper sysadmin 5 November 2019 Although some digital platforms now have an impact on more people’s lives than does any one state authority, the international community has been slow to hold to account these platforms’ activities by reference to human rights law. — A man votes in Manhattan, New York City, during the US elections on 8 November 2016. Photo: Getty Images. This paper examines how human rights frameworks should guide digital technology. Summary Online political campaigning techniques are distorting our democratic political processes. These techniques include the creation of disinformation and divisive content; exploiting digital platforms’ algorithms, and using bots, cyborgs and fake accounts to distribute this content; maximizing influence through harnessing emotional responses such as anger and disgust; and micro-targeting on the basis of collated personal data and sophisticated psychological profiling techniques. Some state authorities distort political debate by restricting, filtering, shutting down or censoring online networks. Such techniques have outpaced regulatory initiatives and, save in egregious cases such as shutdown of networks, there is no international consensus on how they should be tackled. Digital platforms, driven by their commercial impetus to encourage users to spend as long as possible on them and to attract advertisers, may provide an environment conducive to manipulative techniques. International human rights law, with its careful calibrations designed to protect individuals from abuse of power by authority, provides a normative framework that should underpin responses to online disinformation and distortion of political debate. Contrary to popular view, it does not entail that there should be no control of the online environment; rather, controls should balance the interests at stake appropriately. The rights to freedom of thought and opinion are critical to delimiting the appropriate boundary between legitimate influence and illegitimate manipulation. When digital platforms exploit decision-making biases in prioritizing bad news and divisive, emotion-arousing information, they may be breaching these rights. States and digital platforms should consider structural changes to digital platforms to ensure that methods of online political discourse respect personal agency and prevent the use of sophisticated manipulative techniques. The right to privacy includes a right to choose not to divulge your personal information, and a right to opt out of trading in and profiling on the basis of your personal data. Current practices in collecting, trading and using extensive personal data to ‘micro-target’ voters without their knowledge are not consistent with this right. Significant changes are needed. Data protection laws should be implemented robustly, and should not legitimate extensive harvesting of personal data on the basis of either notional ‘consent’ or the data handler’s commercial interests. The right to privacy should be embedded in technological design (such as by allowing the user to access all information held on them at the click of a button); and political parties should be transparent in their collection and use of personal data, and in their targeting of messages. Arguably, the value of personal data should be shared with the individuals from whom it derives. The rules on the boundaries of permissible content online should be set by states, and should be consistent with the right to freedom of expression. Digital platforms have had to rapidly develop policies on retention or removal of content, but those policies do not necessarily reflect the right to freedom of expression, and platforms are currently not well placed to take account of the public interest. Platforms should be far more transparent in their content regulation policies and decision-making, and should develop frameworks enabling efficient, fair, consistent internal complaints and content monitoring processes. Expertise on international human rights law should be integral to their systems. The right to participate in public affairs and to vote includes the right to engage in public debate. States and digital platforms should ensure an environment in which all can participate in debate online and are not discouraged from standing for election, from participating or from voting by online threats or abuse. 2019-11-05-Online-Disinformation-Human-Rights (PDF) Full Article
ng Subsidies and Sustainable Agriculture: Mapping the Policy Landscape By www.chathamhouse.org Published On :: Tue, 10 Dec 2019 18:36:21 +0000 Subsidies and Sustainable Agriculture: Mapping the Policy Landscape Research paper sysadmin 10 December 2019 Agricultural subsidies shape production and consumption patterns, with potentially significant effects on poverty, nutrition and other sustainability concerns. This paper maps the different types of support provided by governments to the agricultural sector, and highlights some of the complex political economy dynamics that underpin the relevant policies. — Aerial view of a wheat field on 24 May 2019 in Linyi, Shandong Province of China. Photo: Getty Images. Summary Agricultural subsidies, a mainstay of government policy, have a large part in shaping production and consumption patterns, with potentially significant effects as regards poverty, food security, nutrition, and other sustainability concerns such as climate change, land use practices and biodiversity. There are multiple types of direct and indirect support provided by governments to various actors in the agricultural sector; and in terms of political economy, there are complex dynamics underpinning the policies that sustain these subsidies. Overall, subsidies targeting producers have the most significant effect on production, and the greater trade-distorting effect. These subsidies promote domestic production and discourage imports, leading to overproduction that is largely disposed of on the international market, with the help of export subsidies. This can tend to intensify negative environmental agricultural practices, such as cultivating marginal land, unsustainable types of intensification, or incentivizing excessive pesticide and fertilizer use. On the other hand, producer subsidies that are not tied to output of a specific commodity (i.e. delinked) have far fewer distorting impacts and could help to deliver sustainable outcomes. For example, this type of subsidies can require crop diversification or be linked to conservation of permanent grassland. Subsidies that enable transfers to consumers, for example through food stamp programmes, also serve to delink production from consumption, can foster healthier diets, can play an important role in delivering food accessibility and security among low-income groups, and can represent one of the less trade-distorting subsidies. If subsidies are to be reformed to help promote healthier diets and encourage more sustainable production, it is essential to understand not only the type and amount of support that key countries provide, but also the domestic dynamics that can shape such policies. While price support, input subsidies or investment aids remain the central pillars of programmes in large developing countries such as Brazil, China or India, other economies – notably including the EU and Japan – focus on direct payments, support for general services and set-aside schemes, as well as significant border protection. The US, for its part, has tended to focus on subsidized insurance schemes and food programmes for poorer consumers. If subsidies are to deliver policy objectives, their design and implementation should delink production from consumption. For example, consumer subsidies designed to deliver nutrition and food security, or payments for environmental services to enable more environmentally friendly production systems, could prove to be the most effective, least trade-distorting means of achieving more sustainable and equitable agricultural production. The political economy of food means that the removal of subsidies is often highly sensitive, and tends to be met with significant resistance. However, reform that delinks support from production through a gradual transition process could ultimately prove successful in delivering effective subsidy schemes. Effective subsidy schemes must by design be truly result- and performance-based, supported by robust and objective indicators. At the same time, engaging multiple actors along key commodity value chains – including leading importing and exporting countries, traders and transporters – could lead to the development of international, commodity-specific arrangements that are able to deliver effective nutrition and sustainability goals. Subsidies and Sustainable Ag - Mapping the Policy Landscape FINAL-compressed (PDF) Full Article
ng Centenary Series: Exploring the International Affairs Archive By www.chathamhouse.org Published On :: Mon, 14 Sep 2020 09:18:37 +0000 Centenary Series: Exploring the International Affairs Archive dora.popova 14 September 2020 International Affairs has been a central part of the institute’s history, both as a record of speeches made by dignitaries such as Mahatma Gandhi and Henry Kissinger, and as a forum for policy-relevant academic research. Delving into the International Affairs archive brings out stories behind some of the most significant players of the last century. Full Article
ng Diagnosing social behavioural dynamics of corruption By www.chathamhouse.org Published On :: Wed, 08 Dec 2021 16:52:08 +0000 Diagnosing social behavioural dynamics of corruption Other resource dora.popova 8 December 2021 This interactive toolkit identifies the types of social expectations which sustain selected corrupt practices and provides behaviourally-informed recommendations for changing them. When tackling a problem as complex as corruption, it is important to understand why and how people behave in different situations where corruption occurs. In contexts where it is easier to engage in corruption than avoid it, identifying the social expectations and informal rules which sustain corrupt practices can advance corruption prevention and deepen collective action. Behavioural approaches to corruption offer a better understanding of diverse social settings, group dynamics, power distribution, social motivations, and expectations that contribute to a more tolerant environment for certain forms of the phenomenon. They are also highly complementary to traditional corruption measures, which tend to focus on the enforcement of legal sanctions and deterrents. Behavioural approaches, especially those inspired by social norms theory, highlight complex social characteristics and informal rules of specific corrupt practices, and effectively support implementation of more dynamic context-specific anti-corruption interventions. Since 2016, the Chatham House Africa programme’s Social Norms and Accountable Governance (SNAG) project has adopted a behavioural approach based on social norms methodology to investigate the social beliefs which motivate different forms of corruption. Drawing on the project’s extensive evidence-gathering and analysis, this toolkit offers users navigable behavioural mapping of contextual factors, beliefs, and expectations surrounding common corrupt practices. It aims to support anti-corruption actors in diagnosing informal rules and social expectations which sustain corruption in some societies. It also proposes behavioural-informed guidance for developing or adapting anti-corruption interventions and activities, so they account for informal rules of behaviour such as social norms. The toolkit supports users to: Identify whether and how widespread corrupt practices are motivated by social beliefs and expectations. Understand how society influences the types of corrupt activity individuals engage in, or avoid, and the factors informing these choices Integrate empirical evidence and behavioural insights into anti-corruption strategies from diagnostics to design, and eventual implementation and evaluation The toolkit presents evidence from SNAG’s research into three key corrupt practices – bribery, embezzlement, and electoral fraud. Each was examined in the context of typical situations in which they occur, such as law enforcement, healthcare, the power sector, voting, and education while critical factors such as religion, gender, and ethnicity were considered. The toolkit presents an overview of specific contexts and behavioural features of the practices and provides behavioural-informed recommendations. It also contains pop-up features with definitions and explanations of key concepts. The toolkit is designed to be expandable, allowing further content and behavioural dynamics to be added. Full Article
ng To be there when the picture is being painted [Protein Structure and Folding] By www.jbc.org Published On :: 2020-11-20T00:05:59-08:00 There is nothing quite like the excitement of discovery in science—of finding something no one else knew and seeing a story unfold. One has to be part of an emerging picture to feel the elation. These moments in a lifetime are few and far between, but they fuel enthusiasm and keep one going. They are embedded in struggles and joys of everyday life, years of establishing what Louis Pasteur called “the prepared mind,” working with mentors, trainees, and colleagues, failures and successes. This article recalls 1) how I got to be a biochemist; 2) my contributions as an educator and researcher, especially regarding meprin metalloproteases; and 3) my participation in communities of science. Perhaps my reflections will help an aspiring scientist see how fulfilling a career in science can be. Full Article
ng Mycobacteria excise DNA damage in 12- or 13-nucleotide-long oligomers by prokaryotic-type dual incisions and performs transcription-coupled repair [Genomics and Proteomics] By www.jbc.org Published On :: 2020-12-11T00:06:21-08:00 In nucleotide excision repair, bulky DNA lesions such as UV-induced cyclobutane pyrimidine dimers are removed from the genome by concerted dual incisions bracketing the lesion, followed by gap filling and ligation. So far, two dual-incision patterns have been discovered: the prokaryotic type, which removes the damage in 11–13-nucleotide-long oligomers, and the eukaryotic type, which removes the damage in 24–32-nucleotide-long oligomers. However, a recent study reported that the UvrC protein of Mycobacterium tuberculosis removes damage in a manner analogous to yeast and humans in a 25-mer oligonucleotide arising from incisions at 15 nt from the 3´ end and 9 nt from the 5´ end flanking the damage. To test this model, we used the in vivo excision assay and the excision repair sequencing genome-wide repair mapping method developed in our laboratory to determine the repair pattern and genome-wide repair map of Mycobacterium smegmatis. We find that M. smegmatis, which possesses homologs of the Escherichia coli uvrA, uvrB, and uvrC genes, removes cyclobutane pyrimidine dimers from the genome in a manner identical to the prokaryotic pattern by incising 7 nt 5´ and 3 or 4 nt 3´ to the photoproduct, and performs transcription-coupled repair in a manner similar to E. coli. Full Article
ng Nonspecific DNA binding by P1 ParA determines the distribution of plasmid partition and repressor activities [Microbiology] By www.jbc.org Published On :: 2020-12-11T00:06:21-08:00 The faithful segregation, or “partition,” of many low-copy number bacterial plasmids is driven by plasmid-encoded ATPases that are represented by the P1 plasmid ParA protein. ParA binds to the bacterial nucleoid via an ATP-dependent nonspecific DNA (nsDNA)-binding activity, which is essential for partition. ParA also has a site-specific DNA-binding activity to the par operator (parOP), which requires either ATP or ADP, and which is essential for it to act as a transcriptional repressor but is dispensable for partition. Here we examine how DNA binding by ParA contributes to the relative distribution of its plasmid partition and repressor activities, using a ParA with an alanine substitution at Arg351, a residue previously predicted to participate in site-specific DNA binding. In vivo, the parAR351A allele is compromised for partition, but its repressor activity is dramatically improved so that it behaves as a “super-repressor.” In vitro, ParAR351A binds and hydrolyzes ATP, and undergoes a specific conformational change required for nsDNA binding, but its nsDNA-binding activity is significantly damaged. This defect in turn significantly reduces the assembly and stability of partition complexes formed by the interaction of ParA with ParB, the centromere-binding protein, and DNA. In contrast, the R351A change shows only a mild defect in site-specific DNA binding. We conclude that the partition defect is due to altered nsDNA binding kinetics and affinity for the bacterial chromosome. Furthermore, the super-repressor phenotype is explained by an increased pool of non-nucleoid bound ParA that is competent to bind parOP and repress transcription. Full Article
ng Optimized incorporation of an unnatural fluorescent amino acid affords measurement of conformational dynamics governing high-fidelity DNA replication [DNA and Chromosomes] By www.jbc.org Published On :: 2020-12-11T00:06:21-08:00 DNA polymerase from bacteriophage T7 undergoes large, substrate-induced conformational changes that are thought to account for high replication fidelity, but prior studies were adversely affected by mutations required to construct a Cys-lite variant needed for site-specific fluorescence labeling. Here we have optimized the direct incorporation of a fluorescent un-natural amino acid, (7-hydroxy-4-coumarin-yl)-ethylglycine, using orthogonal amber suppression machinery in Escherichia coli. MS methods verify that the unnatural amino acid is only incorporated at one position with minimal background. We show that the single fluorophore provides a signal to detect nucleotide-induced conformational changes through equilibrium and stopped-flow kinetic measurements of correct nucleotide binding and incorporation. Pre-steady-state chemical quench methods show that the kinetics and fidelity of DNA replication catalyzed by the labeled enzyme are largely unaffected by the unnatural amino acid. These advances enable rigorous analysis to establish the kinetic and mechanistic basis for high-fidelity DNA replication. Full Article
ng Predicting the Phillies' Opening Day roster By mlb.mlb.com Published On :: Sun, 10 Feb 2019 16:19:58 EDT Phillies pitchers and catchers have their first workout Wednesday morning at Carpenter Complex. Almost anything can happen between Wednesday and Opening Day. That said, here is a very early prediction of the Phillies' Opening Day roster, knowing the Phillies remain in the hunt to sign Bryce Harper or Manny Machado. Full Article
ng Franco, Kingery vie for ABs; Arrieta recovering By mlb.mlb.com Published On :: Wed, 13 Feb 2019 16:18:59 EDT A slimmed down Maikel Franco and a bulked up Scott Kingery will compete for playing time at third base this season. Full Article
ng Manny? Harper? Klentak satisfied but monitoring By mlb.mlb.com Published On :: Thu, 14 Feb 2019 15:13:21 EDT General Manager Matt Klentak discussed the Phillies' offseason in a press conference on Thursday in Clearwater, Fla. The Phillies remain in contact with the agents for Bryce Harper and Manny Machado. The belief is that the front office still prefers Machado over Harper because of Machado's combination of offense and defense. Full Article
ng Segura moving forward with contending Phillies By mlb.mlb.com Published On :: Sat, 16 Feb 2019 18:17:05 EDT Jean Segura became one of the Phillies' most notable offseason acquisitions, a two-time All-Star expected to give the organization its best overall production at shortstop since Jimmy Rollins left town, in part because of a little brawl he had late last season with Mariners teammate Dee Gordon. Full Article
ng Franco ignoring rumors, ready for 3B battle By mlb.mlb.com Published On :: Sun, 17 Feb 2019 17:58:44 EDT Maikel Franco followed the endless and often mind-numbing speculation the past few months from the Dominican Republic. Franco knows that Manny Machado could show up at Spectrum Field at any moment. The Phillies entertained him at Citizens Bank Park just before Christmas. They have made him at least one contract offer. If the Phillies sign him, Franco also knows he could be traded. Full Article
ng The Phillies' Spring Training battle to watch By mlb.mlb.com Published On :: Mon, 18 Feb 2019 18:20:16 EDT The next five weeks will see lots of shuffling on Major League rosters. Here are the most intriguing positional battles on each of the 30 MLB clubs. Full Article
ng Talks 'intensifying' between Harper, Phillies By mlb.mlb.com Published On :: Mon, 18 Feb 2019 11:26:49 EDT The Phillies have been the favorites in the Bryce Harper sweepstakes for months, and that speculation heated up over the weekend. Full Article
ng Hoskins would love to play alongside Harper By mlb.mlb.com Published On :: Tue, 19 Feb 2019 17:35:23 EDT Will Rhys Hoskins be bummed if Bryce Harper signs elsewhere? Full Article
ng Angels prospect to watch in camp By mlb.mlb.com Published On :: Mon, 4 Feb 2019 21:10:42 EDT The countdown to pitchers and catchers reporting is down to single digits for all 30 MLB clubs, but as exciting as it is to see the return of Major League stars, it's also a time to dream about the next wave of baseball talent. Full Article
ng Around the Horn: Angels eye improved bullpen By mlb.mlb.com Published On :: Mon, 4 Feb 2019 14:50:41 EDT Leading up to the start of Spring Training, the Around the Horn series will examine each of the Angels' positional groupings heading into the 2019 season. Here's a look at the bullpen. Full Article
ng Here's your guide to Angels Spring Training By mlb.mlb.com Published On :: Wed, 6 Feb 2019 13:55:04 EDT It's almost time for Spring Training yet again, as Angels pitchers and catchers report to their Spring Training complex in Tempe, Ariz. on Feb. 12. Full Article
ng Inbox: Which Angels will surpass expectations? By mlb.mlb.com Published On :: Thu, 7 Feb 2019 10:00:00 EDT With less than a week left until Angels pitchers and catchers report to Spring Training, it's time for the final Angels Inbox of the offseason. Full Article
ng Previewing the AL West's biggest questions By mlb.mlb.com Published On :: Fri, 8 Feb 2019 14:57:02 EDT Our weekly series previewing each of baseball's six divisions begins with the American League West. Let's take a team-by-team look at the biggest questions this season. Full Article
ng Angels enter '19 optimistic for present, future By mlb.mlb.com Published On :: Fri, 8 Feb 2019 15:41:44 EDT Since debuting in 2011, center fielder Mike Trout has been far and away the best player in the Majors, but the Angels have reached the postseason just once in his eight-year career. Full Article
ng Angels' hope: Ohtani returns as DH in May By mlb.mlb.com Published On :: Tue, 12 Feb 2019 16:21:13 EDT Brad Ausmus held his first media session of the spring on the day pitchers and catchers officially reported on Tuesday, giving updates on rehabbing players such as Shohei Ohtani, Albert Pujols and Zack Cozart. Full Article
ng Simmons ranks among game's best By mlb.mlb.com Published On :: Sat, 9 Feb 2019 20:00:00 EDT MLB Network's countdown of baseball's best players at each position continued with the third installment of the "Top 10 Right Now!" series, featuring the game's top left and center fielders. Full Article
ng Predicting the Angels' Opening Day roster By mlb.mlb.com Published On :: Mon, 11 Feb 2019 11:19:00 EDT The Angels head to Spring Training with most of their roster set, but there will be competition for a few spots. Here's a look at the projected roster for the Angels, as they begin their first campaign under manager Brad Ausmus. Full Article
ng Halos announce Minor League coaching staffs By mlb.mlb.com Published On :: Sat, 16 Feb 2019 15:14:58 EDT The Angels announced their Minor League coaching staffs on Saturday, including former Major League catcher Lou Marson being named the new manager of Triple-A Salt Lake and former big leaguer David Newhan as the new skipper at Double-A Birmingham. Full Article
ng Angels ink veteran Jennings to Minors deal By mlb.mlb.com Published On :: Sat, 16 Feb 2019 13:45:00 EDT The Angels added a veteran lefty reliever, as Dan Jennings officially signed a Minor League deal on Saturday and joined the club for workouts at the club's Spring Training complex. Jennings will earn $1 million, plus incentives, if he makes the club. Full Article
ng Angels' Top 30 prospects list By m.mlb.com Published On :: Wed, 28 Feb 2018 15:07:56 EDT Who do the Angels have in the pipeline? Get scouting reports, video, stats, projected ETAs and more for the Halos' Top 30 Prospects on MLB Pipeline's Prospect Watch. Full Article
ng Ohtani focused on next step of hitting off tee By mlb.mlb.com Published On :: Sun, 17 Feb 2019 14:55:01 EDT Shohei Ohtani is continuing to make progress in his rehab from Tommy John surgery. On Sunday, he said he is hopeful he'll be able to start hitting off a tee in the next week. Full Article
ng Pujols healthy, aims to be ready Opening Day By mlb.mlb.com Published On :: Sun, 17 Feb 2019 14:35:59 EDT Angels first baseman Albert Pujols met with the media for the first time this spring on Sunday and said he's fully healthy after undergoing arthroscopic surgery on his left knee in late August. Full Article
ng The Angels' Spring Training battle to watch By mlb.mlb.com Published On :: Mon, 18 Feb 2019 18:20:16 EDT The next five weeks will see lots of shuffling on Major League rosters. Here are the most intriguing positional battles on each of the 30 MLB clubs. Full Article
ng Angels, Lucroy ready to make adjustments By mlb.mlb.com Published On :: Tue, 19 Feb 2019 17:17:39 EDT The Angels believe they can help newly acquired catcher Jonathan Lucroy improve both offensively and defensively. Full Article
ng Whooping cough: Health officials urge pregnant women to get vaccinated as another infant dies By www.bmj.com Published On :: Friday, August 9, 2024 - 12:56 Full Article
ng Whooping cough: Why have vaccination rates plummeted in pregnant women? By www.bmj.com Published On :: Thursday, August 29, 2024 - 14:36 Full Article
ng Why are doctors being warned about the Oropouche virus? By www.bmj.com Published On :: Thursday, September 5, 2024 - 14:21 Full Article
ng Covid inquiry: UKHSA chief is challenged on view that evidence for FFP3 masks is “weak” By www.bmj.com Published On :: Monday, September 23, 2024 - 08:51 Full Article
ng Whooping cough: Fivefold rise in US cases spells return to pre-pandemic levels By www.bmj.com Published On :: Thursday, September 26, 2024 - 10:56 Full Article
ng Politicians are failing to prepare for next pandemic, warns head of European health agency By www.bmj.com Published On :: Thursday, September 26, 2024 - 11:21 Full Article
ng Mpox is accelerating antimicrobial resistance in Africa, officials warn By www.bmj.com Published On :: Friday, September 27, 2024 - 10:57 Full Article
ng Pandemics are no longer “rare” and now pose constant threat, global preparedness board warns By www.bmj.com Published On :: Tuesday, October 15, 2024 - 14:51 Full Article
ng Targeted TB screening could help halt rise in cases, says Public Health Scotland By www.bmj.com Published On :: Thursday, October 24, 2024 - 15:26 Full Article
ng Preserving fertility in girls and young women with cancer By www.bmj.com Published On :: Wednesday, November 30, 2016 - 23:30 Full Article
ng Constitutional symptoms in a young person By www.bmj.com Published On :: Wednesday, December 7, 2016 - 10:05 Full Article
ng Anti-bullying programme is launched by orthopaedic trainees By www.bmj.com Published On :: Monday, January 16, 2017 - 08:00 Full Article
ng Choosing a core surgical training interview skills course By www.bmj.com Published On :: Tuesday, August 8, 2017 - 07:00 Full Article